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Diploma Track Title : Nutraceuticals

Diploma Track Title : Nutraceuticals


Preetha Bhadra


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Diploma Track Title : Nutraceuticals

Track Total Credits ( T-P-P): 3-12-9 (24 Credits)


Type of course: Diploma Course Track and Practice

Eligibility: 10th/12th/ITI

Pre-requisites: Participants would have some idea regarding the basics of biology and interest in the field of nutrition.

Duration of the course:

Class: 12o hours of classes

Practical: 90 hours (Practice to be done on campus and outside campus)

Project: 120 hours of project work

Key Highlights

  • Participants will get a certificate from the university after successful completion.
  • Participants can work on different medicinal plant extracts
  • Participants can work on different drying mechanism of the food
  • Participants can have an industrial experience of the CO2 extraction plant
  • Participants can also have experience working in both plant and animal models.
  • Imparts knowledge for development as an entrepreneur 

Course Objectives

  • To study the advantages of functional foods over conventional Medicine to avoid potential side-effects
  • To Study dietary supplements
  • To Study about food gene interface
  • To distinguish between food, functional food, and supplements
  • To learn about Drug Designing,
  • To learn about detailed Protein structure, pharmacophore, basics of Docking and Discovery studio and application in Agriculture, Fisheries and others Science.

Learning Outcomes

  • To understand Functional foods and their effects on human health
  • To understand the role of antioxidants, polyphenols, omega-3 fatty acids, to prevent different physiological disorders.
  • To Understand the importance of personalized food with respect to nutrigenetics.
  • To be able to design a drug molecule and identify its applications
  • To be able to analyze nanomaterials and use it as Bio-Pesticides and Biofertilizers,
  • To be able to design a drug molecule and identify its applications.
  • To be able to illustrate the structure of Protein, Pharmacophores

Course Division

Track courses (3-6-0)

  1. Introduction to Nutraceutical (1+2)
  2. Functional Food (1+2)
  3. Nutrigenetics (1+2)

Project Courses (0-6-9)

  1. Development of Personalized Food  and Medicine (0-2-3)
  2. Development of Biopesticides and Biofertilizers  (0-2-3)
  3. Development of Immune Boosters (0-2-3)

Course Syllabus:

Track Course 1: Introduction to Nutraceutical (1+2)

Organizational elements, classification of nutraceuticals, dietary supplements, fortified foods, functional foods, and Phyto-nutraceuticals. The scope involved in the industry, Indian, and global scenario, Food Chemistry, Preservatives, food colors.


  1. Milk Analysis: Determination of fat, acidity, lactose, protein, adulterants
  2. Analysis of water: Collection of the sample, preliminary examination, Total soluble solids, Determination of total hardness, chlorides, dissolved oxygen
  3. Analysis of fats: acid value, saponification value, iodine number, antioxidants
  4. Analysis of foods: Determination of reducing and non reducing sugar, protein, determination of ash/total protein/moisture in dietary fibers
  5. Anti-nutritional Factors: Trypsin inhibitor, Phytic acid, Antifungal activity of cystatin
  6. Vitamins: Ascorbic acid by colorimetric method, estimation of Vit A/E
  7. Pigments: Curcumin, chlorophyll, carotene
Track Course 2: Functional Food (1+2)
  • Prebiotics-   Definition, chemistry, sources, metabolism and bioavailability, the effect of processing, physiological effects, effects on human health, and potential applications in risk reduction of diseases. perspective for food applications for the – Non-digestible carbohydrates/oligosaccharides, Dietary fiber, Resistant starch, Gums.
  • Probiotics- Taxonomy and important features of probiotic microorganisms. Health effects of probiotics including mechanism of action. Probiotics in various foods: fermented milk products, non-milk products, etc. Quality Assurance of probiotics and safety.
  • Drug Discovery - Issues, Target and Lead Identification, Drug And Databases, Drug Properties, Drug Solubility/permeability, ADME, Molecular Modelling, Quantitative Structure-Activity Relationship, Target-based drug design, Docking. Pharmacokinetics/ pharmacodynamics, Discussion on Molecular Drug Designing, Targeted drug designing, Application


  1. Extraction Techniques: Soxhlet Extraction, Cold Press Extraction, Super Critical Fluid Extraction, CO2 Extraction
  2. Isolation and Purification of Phytochemicals: TLC, Paper Chrom, Column Chrom, HPLC, Analysis of Aflatoxin
  3. Validation of Phytochemicals: Using different microorganisms and mammalian cell lines

Track Course 3: Nutrigenetics (1+2)


Introduction to Nutrigenetics, Nutrigenomics, Concept of Personalized food with special references to athletes. Introduction to different types of public domain databases, data mining strategies, primer designing.


  1. m-RNA extraction from treated mammalian cell line
  2. Preparation of c-DNA
  3. Study of Gene expression in Real-Time PCR
  4. Electrophoresis

Project Course 1: Development of Personalized Food  and Medicine

  • ANTIOXIDANTS: Concept of free radicals and antioxidants; antioxidants role as nutraceuticals and functional foods.
    FOOD AS REMEDIES: Nutraceuticals bridging the gap between food and drug; nutraceuticals for specific situations such as cancer, heart disease, diabetes, stress, osteoarthritis, hypertension; nutraceutical remedies for common disorders like arthritis, bronchitis, circulatory problems, hypoglycemia, liver disorders, osteoporosis, psoriasis, and ulcers, etc.

Project Course 2: Development of Biopesticides and Biofertilizers

  • Biofertilizers: Introduction and types and importance of biofertilizers, Biopesticides, and bioagents in agriculture and organic farming system, History of biofertilizer production Classification of biofertilizers microorganisms used in biofertilizers production. A study of growth characteristics of various microbes used in biofertilizer production.
  • The nitrogen cycle in Nature. Process of nodule formation, Role of Nif and Nod gene in Biological Nitrogen fixation, Enzyme nitrogenase, and its component, Biochemistry of nitrogen fixation, Cross inoculation groups amongst Rhizobium, Methods used for the studying selection of efficient strain of Rhizobium.
  • Quality standard for biofertilizers different methods of application of biofertilizers, the role of microorganisms in the decomposition of organic farm wastes, methods of quality control assessment in respect of biofertilizers, Strategies of Mass multiplication, and packing Registration of biofertilizers. Strategies of marking and Registration with CIB of bioagents and biopesticides Importance of Trichoderma spp., Pseudomonas spp. and Bacillus spp. as biocontrol agents, Mechanism of disease control by these organisms bioagents.
  • Types of diseases controlled bioagents formulations, Effectiveness of bioagents against seed-borne and soil-borne plant pathogens, Mass multiplication and packing, Strategies of marking, and Registration with CIB and organic farming institute Importance of Trichogramma, Cryptolaemus, Chrysoperla, NPV, and entomofungal pathogens. Establishing insectary for host insects and natural enemies, Mass production of Verticillium/Beauveria/Metarhzium/Nomuraea/Paecilomyces/Hirsutella thompsoni/Trichoderma, / Pseudomonas/Bacillus/Potash Mobilizers/Sulphur oxidizers /organic matter decomposers

Project Course 3: Development of Immune Boosters

  • From early in evolution, organisms had to protect themselves from pathogens. Mechanisms for discriminating self from non-self evolved to accomplish this task, which launched a long history of host-pathogen co-evolution. The evolution of mechanisms for immune defense has resulted in a variety of strategies. Prokaryotes use restriction endonucleases, antimicrobial peptides, and RNA interference for self-protection.
  • In multicellular organisms, specialized immune cells have evolved, capable of the phagocytosis of foreign cells as well as of self cells changed by infection or cancer. Defensive mechanisms based upon germline-encoded receptors constitute a system of innate immunity.
  • In jawed vertebrates, this system is supplemented with a second system, known as adaptive immunity, which involves a specialized network of immune cells and organs. Adaptive immunity, in contrast to innate immunity, is based on the diversification of immune receptors and immunological memory in each individual. In this course, we will analyze evolutionary pathways that have led to the development of innate and adaptive immunity, trace both the conserved and unique features of the immune response from bacteria to higher vertebrates, and identify factors, such as adaptive changes in pathogens that have shaped the evolution of the immune system.

Text Books

Introduction to Nutraceuticals:

  • Giuseppe Mazza; Functional Foods: Biochemical and Processing Aspects, Volume 1; CRC Press
  • Robert E.C. Wildman; Handbook of Nutraceuticals and Functional Foods, Second Edition; CRC Press
  • Massimo Maffei; Dietary Supplements of Plant Origin; CRC Press
  • Fereidoon Sahidi, Deepthi K. Weerasinghe; Nutraceutical Beverages, Chemistry, Nutrition and Health Effects; American Chemical Society

Functional Foods:

  • Ronald R. Watson; Vegetables, Fruits, and Herbs in Health Promotion; CRC Press
  • Fruit and Cereal Bioactives: Sources, Chemistry and Applications; ÖzlemTokusoglu; Clifford Hall III; CRC Press
  • Susan Sungsoo Cho, Mark L. Dreher; Marcel; Dekker Handbook of Dietary Fibre


  • Principles of Nutrigenetics and Nutrigenomics - 1st Edition. Print Book & E-Book. ISBN 9780128045725, 9780128045879.
  • Nutrigenetics: Applying the Science of Personal Nutrition. Academic Press; Reprint edition (19 August 2016).
  • Nutrigenetics and Nutrigenomics. World Review of Nutrition and Dietetics, Vol. 93

Development of Personalized Food and Medicine

Textbook of Personalized Medicine. Authors: Jain, K. K. Springer Publication

Development of Biopesticides and Biofertilizers

Biofertilizers and Biopesticides Paperback – 29 June 2019 by Krishnendu Acharya (Author), Surjit Sen (Author), Manjula Rai (Author)

Biofertilizer and Biopesticide Hardcover – 1 March 2011, by Shalini Suri (Author)

Development of Immune Boosters

Immune System: How To Boost The Immune System Naturally by Elizabeth Grace

Session Plan for Track courses

Session Plan:  1.1.1

Classification of nutraceuticals

classification of nutraceuticals

Session Plan:  1.1.2

Organizational elements

Organization of Elements

Session Plan:  1.1.4



Session Plan:  1.1.5

The scope involved in the industry

Nutraceuticals Market_Global Forecast

Session Plan:  1.1.6

Food Chemistry

Food chemistry

Session Plan:  1.1.7

Food Colors

Food colours

Session Plan:  1.1.8



Session Plan:  1.1.9

Regulation of Dietary Supplements

Regulation of Dietary Supplements

Session Plan:  1.2.1

Milk Analysis: Determination of fat, acidity, lactose, protein, adulterants

Session Plan:  1.2.2

Analysis of water: Collection of the sample, preliminary examination, Total soluble solids, Determination of total hardness, chlorides, dissolved oxygen

Session Plan:  1.2.3

Analysis of fats: acid value, saponification value, iodine number, antioxidants

Session Plan:  1.2.4

Analysis of foods: Determination of reducing and non-reducing sugar, protein, determination of ash/total protein/moisture in dietary fibers

Session Plan:  1.2.5

Anti-nutritional Factors: Trypsin inhibitor, Phytic acid, Antifungal activity of cystatin

Session Plan:  1.2.6

Vitamins: Ascorbic acid by colorimetric method, estimation of Vit A/E

Session plan for Project Courses: (Any two)

2.1. To develop personalized food and beverages rich in Antioxidants and Polyphenols

This project includes the extraction of phytochemicals, identification of bioactive compound(s), isolation of this compound, and incorporation in food/beverages. This food/beverages will be specific for people suffering from hyperglycemia, obesity, or may target athletes to provide instant energy.

2.2. To develop low-cost omega-3 capsules from fish oil/ walnuts

PUFAs like omega-3 fatty acids found in fish oil, walnuts can reduce inflammation and improve hypertriglyceridemia. Formulation of this type of capsule involves drug designing (using Discovery Studio, Biovia) and clinical trials.

2.3. To develop immune boosters

Immune boosters are most suitable for children below 10 years and the aged person above 60 years. This will strengthen the internal immunity of the body and will help to combat the common cold viruses, cases of flu, etc.

2.4. To develop protein bars

Protein bars are popular among the young generation as they are healthy and tasty. Low-calorie protein bars, rich with fruits and nuts may be a popular product with high market value. The level of carbohydrate, fats, proteins, vitamins need to be standardized and hence basic food biochemistry analysis will be involved.

2.5. To develop the extraction of antioxidants and polyphenols from different sources

SCFE (using carbon dioxide) of antioxidants and polyphenols from different sources (eg: Ramie, Dragon Fruit, Berries) can be performed in Gram Tarang Foods Pvt. Ltd. Besides, soxhlet, cold press methods can be explored for finding a commercial way of extraction and supplying it to different food/beverage companies. This project could be beneficial for getting the monetary benefits as well as getting publication by studying the biological efficacy of the plant extracts.

2.6. In-silico Validation of the Phytochemicals on Fish cell line and Plant Microbes
The bioactive molecules from phytochemicals can be used against different types of plant pathogens as well as can be used as fish feed to protect from bacterial or fungal action. This will include molecular docking, pharmacophore modelling. In- silico models will be validated using wet lab tests. Quality publications are expected.

2.7. Drug Designing
Known bioactive molecules from natural resources will be used for drug designing against different human physiological disorders, such as, Hypertension, Hyperglycemia, Parkinson’s disease and certain metabolic disorders. Validation of the drugs warrants clinical trial and may lead to patent and publications.

2.8. In-silico Validation of Nanoparticle dosages on Fish Cell Line and Plant Microbes
Studies on nanoparticles and its applications in plant pathology and fishery sciences are gaining immense popularity. In-silico models will can provide proper experimental design to many research works related to fisheries and plant pathology that would save time, money and man power. Validation of in-silico data in wet lab may lead to quality publications.

Case Studies

Case Studies

Our Main Teachers

Preetha Bhadra

Assistant Professor

PhD in Biophysics, currently working as an Assistant Professor in the Department of Biotechnology. My current working field is the uses of different Medicinal plants and spices and their application in Agriculture and Lifesciences, Drug designing. I love to take challenges and work hard to complete that. New domains and areas of my venture are […]

Present Position Assistant Professor, Dept. of Biochemistry and Plant Physiology, MSSSoA, Centurion University of Technology & Management, Odisha, India Mobile: 9330492088 , E-mail: Education Bachelor of Science (Biotechnology), PES College, Bangalore University, Karnataka, India Master of Science (Biochemistry), Vidya Sagar University, West Bengal, India Ph.D. (Soil Sc. & Agri. Chemistry), Visva Bharati University, Birbhum, […]