Analytical Biochemistry

Teacher

Susmita Chakrabarty

Category

Core Courses

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Course Name : Analytical Biochemistry

Code(Credit) : CUTM1738 (3-2-0)

Course Objectives

  • Understanding the concept of Biochemical analyzing instruments both automated and semi automated.
  • To learn about how to Care & Maintenance of Equipment & Chemicals.
  • To learn normal ranges of biochemical components in our body.
  • Clinically relevant biochemical analysis for deeper understanding of all biochemical components i.e., Proteins, Electrolytes, Hormones etc.

Learning Outcomes

  • Understanding of instrumentation technique & principle of spectrophotometry, colometry, photometry and electrolyte analyzer.
  • To learn about Various tests carried out for biochemical analysis & Hormone investigations.
  • To learn about safety precautions and handling the equipment in biochemical laboratory.

Course Syllabus

Module- I ( 12 Hrs)

 

Chromatography: Paper, Thin layer, Column, Ion exchange, Affinity chromatography, Gel filtration, Gas Chromatography, HPLC, FPLC

Practice: Handling the Equipments and chemicals used in biochemical laboratory.

 

Module-III ( 12 Hrs)

 

Electrophoresis: Moving boundary, Zone (Paper Gel) electrophoresis, Immuno electrophoresis, Isoelectric focusing, 2-D electrophoresis. Principle, Instrumentation, Specimen preparation and Application of: X-ray diffraction, NMR, ESR

Practice: Estemate Erythrocyte sedimentation rate

 

Module- III ( 26 Hrs)

 

Principle and Application of: Fully Automated Biochemistry Analyser, Semi- automated Biochemistry Analyser, Coagulometer. Method of estimation and assessment for: a. Glucose tolerance test. Clearance test for renal function. Gastric analysis, LFT, KFT, Lipid profile, Qualitative test for Urobilinogens, Renal calculi, Barbiturates, T3, T4 and TSH, 17 Ketosteroids. Principles, clinical significance and procedures for estimation, of Acid phosphatase, Alkaline phosphatase, Lactate dehydrogenase, Aspartate transaminase, Alanine, transaminase and Creatine phosphokinase.

 

Practice: Glucose Tolerance Test, Clearance Test, Gastric juice collection, Gastric Analysis, Kidney Function Test, Liver Function Test, Lipid Profile,Renal calculi, Hormone Test 

 

 

Suggested readings

  1. Handbook of Christen Medical Association, India (CMAI) Medical Laboratory Technology-Robert H.Carman. 2nd Edn. CMAI, New Delhi.
  2. Text book of Medical Laboratory Technology, P.B. Godkar 2nd Edn. Bhalani Publication.
  3. Handbook of Biochemistry by M. A. Siddique 8th Edn. Vijay Bhagat Scientific Book
  4. Principle of Biochemistry by Lehninger
  5. Biochemistry by Voet&Voet
  6. Biochemistry by Stryer
  7. Biochemistry of Metabolic process by Asim Kumar Roy, Kalyani Publication
  8. Ebook link-https://www.cartercenter.org/resources/pdfs/health/ephti/library/lecture_notes/health_science_students/medicalbiochemistry.pdf
  9. Ebook link-https://books.google.co.in/books?id=Je_pJfb2r0cC&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
  10. Ebook link-https://books.google.co.in/books?id=csPcDAAAQBAJ&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
  11. Ebook link-https://books.google.co.in/books?id=2FkXAwAAQBAJ&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false

 

 

All the practice components can be performed by simulation as well as virtual labs.

Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).

Online Source: Udemy, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia,Slideshare.net

Session 5

Topic: Ion exchange chromatography

Study Materials:

Video- https://www.youtube.com/watch?v=VOSkyj1dtbc

 Notes-https://microbenotes.com/ion-exchange-chromatography/

Session 7

Topic: Gel filtration chromatography

Study Materials: 

Notes-https://microbenotes.com/gel-filtration-chromatography/

Book Reference- Asim Kumar Roy pg:71&72

Session 13

Session 17

Topic: Principle, Instrumentation ,Specimen preparation and Application of: X-ray diffraction

Study Materials:

Notes- https://serc.carleton.edu/research_education/geochemsheets/techniques/XRD.html

Session 20

Topic: Principle and Application of: Fully Automated Biochemistry Analyse

Study Materials:

Video- https://www.youtube.com/watch?v=XD1W4Ak01b4

Session 21

Topic: Principle and Application of: Semi- automated Biochemistry Analyser 

Study Materials:

Video- https://www.youtube.com/watch?v=b69HryqioM0

Session 27

Topic: Gastric analysis

Study Materials: 

Notes- https://jpma.org.pk/article-details/6346

Session 28

Topic: Qualitative test for Urobilinogens

Study Materials: 

Notes- https://medlineplus.gov/lab-tests/urobilinogen-in-urine/

Session 29

Topic: Qualitative test for Barbiturates

Study Materials:

Slide share- https://www.slideshare.net/22suresh/analysis-of-barbiturates

Session 30

Topic: Coagulometer

Video- https://www.youtube.com/watch?v=Mbu6vpcDb84

Session 33

Topic: Principles, clinical significance and procedures for estimation of Acid phosphatase

Study Materials: 

Notes- https://www.encyclopedia.com/medicine/encyclopedias-almanacs-transcripts-and-maps/acid-phosphatase-test

Session 34

Topic: Principles, clinical significance and procedures for estimation of Alkaline phosphatase

Study Materials: 

Notes- https://labtestsonline.org/tests/alkaline-phosphatase-alp

Session 35

Topic: Principles, clinical significance and procedures for estimation of Lactate dehydrogenase

Study Materials: 

Notes- https://kidshealth.org/en/parents/test-ldh.html

Session 36

Topic: Principles, clinical significance and procedures for estimation of Aspartate transaminase

Study Materials: 

Notes- https://www.healthline.com/health/ast

Session 37

Topic: Principles, clinical significance and procedures for estimation of Alanine transaminase

Study Materials: 

Notes- https://www.healthline.com/health/alt

Session 38

Topic: Principles, clinical significance and procedures for estimation of Creatine phosphokinase

Study Materials:

Notes- https://medlineplus.gov/ency/article/003503.htm

Session 41

Practice session:

Topic: Urine (microscopic)

Study Materials: 

Video-https://www.youtube.com/watch?v=DVh9xBuqbSY

Session 42

Practice session:

Topic: Renal calculi

Study Materials: 

Video-https://www.youtube.com/watch?v=-bbv62XKK0E

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      Miss Susmita Chakrabarty is currently working as a Lecturer in School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes field in Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique & Clinical Biochemistry etc. In her research, […]