Analytical Techniques

Teacher

Dr. Soumya Jal

Category

Core Courses

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Course Name : Analytical Techniques

Code(Credit) : CUTM1709 (3-1-0)

Course Objectives

  • To learn the principle, instrumentation & application of Microscopy
  • Principle, instrumentation & application of Centrifugation
  • Chromatographic techniques
  • Electrophoretic techniques
  • Principle of Spectroscopy

Learning Outcomes

  • After completion of the course the student will be efficient in handling the microscopy equipment’s.
  • They will also be able to have idea about handling instruments like centrifuge, spectrophotometer, chromatography, flow cytometer, Automated and semi- automated Biochemistry analyzer.
  • They will gain knowledge on the principle behind and the application of NMR, X-ray diffraction, ESR.
  • The conceptual understanding of the subject provides opportunities for skill enhancement and scopes for higher education.

Course Syllabus

Module–1

Microscopic techniques: Principle, Instrumentation, Specimen preparation and Application: Phase–contrast microscopy, fluorescene microscopy, polarization microscopy, electron microscopy (Scanning and Transmission);

Bacterial Colony Counter (Principle and working).

Microtomy: Sectioning, Staining. Application.

Cytometry: Types, Flow cytometry and its applications.

Practice:

Demonstration of different Microscopes with their operation.

Preparing specimens for observing under microscopes.

Demonstration of Microtome.

 

Module-2

Centrifugation: Principle; Preparative, Analytical, Density gradient centrifugation.

Chromatography: Principles and Applications: Paper, Thin layer, Column, Ion exchange, Affinity chromatography, Gel filtration, Gas Chromatography, HPLC, FPLC.

Electrophoresis: Immuno electrophoresis, Isoelectric focusing, 2-D gel electrophoresis.

Practice:

Demonstration of Centrifuge machine

Demonstration of Chromatography techniques

Demonstration of Electrophoresis

 

Module-3

Colorimeter: Principle and Instrumentation; Spectrophotometry: Ultraviolet, Mass spectrometry; Flame photometry;

Principle, Instrumentation, Specimen preparation and Application of: X-ray diffraction, NMR, EPR. Principle and Application of: Fully Automated Biochemistry Analyser, Semi- automated Biochemistry Analyser, Coagulometer.

Practice:

Demonstration of Semi automated Analyzer

Demonstration of Fully automated Analyzer

 

Suggested Readings:

  1. Wilson K and Walker J. (2010). Principles and Techniques of Biochemistry and Molecular Biology. 7th Ed., Cambridge University Press.

(e-Book link: https://www.pdfdrive.com/principles-and-techniques-of-biochemistry-and-molecular-biology-e174866056.html )

  1. Nelson DL and Cox MM. (2008). Lehninger Principles of Biochemistry, 5th Ed., W.H. Freeman and Company.

(e-Book link: https://www.pdfdrive.com/lehninger-principles-of-biochemistry-5th-edition-d164892141.html )

  1. Willey MJ, Sherwood LM & Woolverton C J. (2013). Prescott, Harley and Klein’s Microbiology. 7thEd., McGraw Hill.

(e-Book link: https://www.pdfdrive.com/prescott-harley-and-kleins-microbiology-7th-ed-e188166539.html )

  1. Labs for Life

(e-source link: http://labsforlife.in/InstructionalVideo.aspx )

 

Simulation links for labs:

Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).

Online Source: Labs for life, Lecturio, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia

Session Plan

Session 1

Topic: Microscopy

Video on Microscopy

https://assetstore.unity.com/packages/templates/packs/cky-microscope-162720

Presentation on Microscopy

Microscopy

Session 2

Topic: Phase–contrast microscopy

Video on Phase–contrast microscopy

https://www.youtube.com/watch?v=fCZw4X7V5Pw

Slideshare link to Phase–contrast microscopy

https://www.slideshare.net/rajpalchoudharyjat/phase-microscopy

Session 3

Session 4

Topic: Polarization microscopy

Link to Polarization microscopy

https://www.microscopyu.com/techniques/polarized-light/polarized-light-microscopy

Session 5

Session 6

Session 7

Practice session

Topic: Demonstration of different Microscopes with their operation and maintain technique

Video on Demonstration of different Microscopes with their operation and maintain technique

https://www.youtube.com/watch?v=RKA8_mif6-E

https://www.youtube.com/watch?v=4x-2GHBel0A

Session 8

Topic: Bacterial Colony Counter

Video on Bacterial Colony Counter

https://www.youtube.com/watch?v=wUIZefiMXxI

https://www.youtube.com/watch?v=cnRGm010KP4

Session 9

Practice session

Topic: Preparing specimens for observing under microscopes

Video on Preparing specimens for observing under microscope

https://www.youtube.com/watch?v=aZzRygip5XY

Procedure on Preparing specimens for observing under microscope

https://bio.libretexts.org/Courses/Portland_Community_College/Cascade_Microbiology/02%3A_How_We_See_the_Invisible_World/2.4%3A_Staining_Microscopic_Specimens

http://www.microbehunter.com/how-can-specimens-be-prepared-for-observation/

Session 10

Topic: Microtomy: Sectioning, Staining, Application

Video on Microtome

http://labsforlife.in/InstructionalVideo.aspx

Session 11

Practice session

Topic: Demonstration of Microtome

Video on Preparing specimens for observing under microscope

https://www.youtube.com/watch?v=KfVizbsuu3k

https://www.youtube.com/watch?v=M1RPnY-pbLg

Session 12

Topic: Cytometry: Types, Flow cytometry and its applications.

Video on Cytometry: Types, Flow cytometry and its applications.

http://labsforlife.in/InstructionalVideo.aspx

Session 13

Session 14

Topic: Preparative centrifuge

Video on Preparative centrifuge

https://www.youtube.com/watch?v=nX0NNfV8nWw

Session 15

Topic: Analytical centrifuge

Video on Analytical centrifuge

https://www.youtube.com/watch?v=A9wmCsMiy70

Session 16

Topic: Density gradient centrifuge

Video on Density gradient centrifuge

https://www.youtube.com/watch?v=_-YT44KP3do

Session 17

Practice session

Topic: Demonstration of Centrifuge machine

Video on Demonstration of Centrifuge machine

https://www.youtube.com/watch?v=NqVaMiTI8Uw

https://www.youtube.com/watch?v=IhJNFGfsUus&t=53s

Session 18

Session 19

Session 20

Practice session

Topic: Demonstration on Paper Chromatography

Video on Demonstration on Paper Chromatography

https://www.youtube.com/watch?v=ZCzgQXGz9Tg&t=64s

Session 21

Session 22

Practice session

Topic: Demonstration on TLC

Video on Demonstration on TLC

https://www.youtube.com/watch?v=lj5OWzhZSac&t=7s

Session 23

Session 24

Practice session

Topic: Demonstration on Column Chromatography

Video on Demonstration on Column Chromatography

https://www.youtube.com/watch?v=UmWMlKJAdSk&t=1s

Session 25

Topic: Ion exchange chromatography

Video on Ion exchange chromatography

https://www.youtube.com/watch?v=wFpLgDceBhkhttps://www.youtube.com/watch?v=q3fMqgT1do8

Slideshare link to Ion exchange chromatography

https://www.slideshare.net/shiva14123/ion-chromatography-55691372

Session 26

Session 27

Session 28

Session 29

Session 30

Session 31

Practice session

Topic: Demonstration of Agarose Gel Electrophoresis

Video on Demonstration of Agarose Gel Electrophoresis

https://www.youtube.com/watch?v=6mQGNDnOyH8&t=11s

https://www.youtube.com/watch?v=KKmiKKMDDhY

https://www.youtube.com/watch?v=vq759wKCCUQ&t=22s

Session 32

Topic: Immuno electrophoresis

Video on Immuno electrophoresis

https://www.youtube.com/watch?v=CzfSUn0U2oQ

https://www.youtube.com/watch?v=52opF5Cc1-k


Session 33

Session 34

Session 35

Session 36

Topic: Colorimeter: Principle and Instrumentation

Video on Colorimeter: Principle and Instrumentation

https://www.youtube.com/watch?v=z78PBaAldRQ

https://www.youtube.com/watch?v=QdufRwbkeKo

https://www.youtube.com/watch?v=yTabfxvMdCM

Slideshare link to Coloroimeter

https://www.slideshare.net/gangadharchatterjee/colorimeter-66390783

Session 37

Session 38

Session 39

Session 40

Session 41

Session 42

Session 43

Topic: Principle and Application of Fully Automated Biochemistry Analyser

Video on Fully Automated Biochemistry Analyser

https://www.youtube.com/watch?v=LHweSWdoQ78

https://www.youtube.com/watch?v=M6HKW4AN4Ug

https://www.youtube.com/watch?v=FFKRNgxvrT0

Session 44

Practice session

Topic: Demonstration of Fully automated Analyzer

Video on Demonstration of Fully automated Analyzer

http://labsforlife.in/InstructionalVideo.aspx

https://www.youtube.com/watch?v=ksCjG_4MXUE

https://www.youtube.com/watch?v=JX_0kPTbzq4

https://www.youtube.com/watch?v=XD1W4Ak01b4

Session 45

Topic: Semi- automated Biochemistry Analyser

Video on Semi- automated Biochemistry Analyser

https://www.youtube.com/watch?v=J9yoeVLxEvA

Session 46

Session 47

Case Studies

Case Studies

Our Main Teachers

Dr. Soumya Jal

Associate Professor
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Dr. Soumya Jal is working at Centurion University of Technology and Management since 2018, prior to which she was associated with Rama Devi Women’s University as a visiting faculty.  She has completed her Ph. D. in Medical Biotechnology from VIT University, Vellore (2016). Her research area includes fields such as, Applied and Clinical Microbiology, Molecular […]