Animal Biotechnology-CUTM2365, Credits (3-0-1)

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• To provide the fundamentals of animal cell culture, details of disease and therapy

  COs	Course outcomes	Mapping COs with POs (High-3, Medium-2, Low-1)
  CO1	Knowledge of animal cells, basics of animal tissue culture technique, knowledge of media, and their fundamentals, preservations etc Understand the detailed functions of the animal cells and their intricate functions	PO1 (3)
  CO4, CO7	Individual and team work: Students will be able to know the animal cell culture and its use for developing valuable products	PO9 (3):
  CO5	Use of practical approach for animal cell culture and its methodology for experiments, analysis, and interpretation of data, in molecular biology	PO2 (3)

   Session Plans Module I Animal Cell Culture Introduction to basic tissue culture techniques; chemically defined and serum free media; animal cell cultures, their maintenance and preservation; various types of cultures; suspension cultures, continuous flow cultures, immobilized cultures;
•   www.youtube.com/watch?v=OBagQf8_lZMModule II Somatic cell fusion                                                                                              Somatic cell fusion; cell cultures as a source of valuable products
• www.youtube.com/watch?v=2XYuwgBnRbg
•   Module III Organ cultures                                                             Information about organs; culture techniques and preservation
• www.youtube.com/watch?v=FNEeX3TJhZI Module IV Animal diseases and their Diagnosis                                                                                                       Bacterial and viral diseases in animals; monoclonal antibodies and their use in diagnosis; molecular diagnostic techniques like PCR, in-situ hybridization; northern and southern blotting; RFLP. Module V Therapy of Animal Diseases                                                                                                       Recombinant cytokines and their use in the treatment of animal infections; monoclonal antibodies in therapy; vaccines and their application in animal infections; gene therapy for animal diseases.
•   www.youtube.com/watch?v=SNm0GtvFYDs
•   www.youtube.com/watch?v=RpGDU5808CAMicromanipulation of Embryo’s          Module VI What is micromanipulation technology; equipment’s used in micromanipulation; enrichment of x and y bearing sperms from semen samples of animals; artificial insemination and germ cell manipulation; invitro fertilization and embryo transfer; micromanipulation technology and breeding of farm animals.
• www.youtube.com/watch?v=r1-J1PaIZfs
•   Module VII Transgenic Animals Concepts of transgenic animal technology;strategies for the production of transgenic animals and their importance in biotechnology; stem cell cultures in the production of transgenic animals
• www.youtube.com/watch?v=RzYhcXjksKcReference
•   Text Books:
  1. Animal biotechnology by P.Ramadas
  2. Embryonic stem cells by Kursad and Turksen. 2002. Humana Press.
   Reference Books
  1. Louis-Marie Houdebine, Transgenic animals: Generation and Use 7^th Edition, CRC  Press

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