ANIMAL BIOTECHNOLOGY (MSc Zoology Core Subject)

Teacher

Dr Bikash Ranjan Sahu

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Subject Code = CUTM 1452

Type of Course - Theory + Practice

Credit - 3+1+0

Course Objectives

  1. To understand the tools and techniques required for the animal cell culture.
  2. To integrate assisted reproductive biotechnology techniques in livestock development.
  3. To utilize animal production technologies for sustainable agriculture and food security.

Course Outcome

COs Course Outcomes Mapping COs with POs

(High-3, Medium-2, Low-1)

CO1 To define methods of culturing animal cells. PO1-2, PO12-2
CO2 To explain the techniques involved in making of transgenic animals. PO12-2, PO3-3
CO3 To implement assisted reproductive biotechnology techniques in livestock improvement. PO3-3, PO10-1
CO4 To utilize animal production technologies for sustainable agriculture and food security. PO1-3, PO9-2
CO5 To formulate technology for integrated farming such as polyculture of fish, edible oyster culture. PO1-3, PO9-3

Course Outline

Module-I (Animal Cell and tissue culture technology-I).

THEORY - Overview of animal cell and tissue culture technology; Cell culture laboratory design and basic laboratory equipment; Media preparation, Role of important components of culture media; Common laboratory hazards and safety issues to consider in cell culture laboratory.

PRACTICE 1 -  Sterilization techniques used in animal cell culture

PRACTICE 2  - Preparation of media for animal cell culture

 

Module-II (Animal Cell and tissue culture technology-II)

THEORY - Types of animal cell culture; Characterization and preservation of animal cells; Advances in cell culture technology; Opportunities and challenges in animal cell and tissue culture technology.

PRACTICE 1 - Study of primary cell culture technique using chick embryo.

PRACTICE 2 - Animal cell batch culture technique.

 

Module-III (Transgenesis for livestock improvement-I)

THEORY - Overview of transgenic technology; Biopharming through animal transgenesis; Methods of producing transgenic farm animals; Identification and transfer of gene influencing better production and disease resistance.

 

Module-IV (Transgenesis for livestock improvement-II)

THEORY - Gene transfer methods in animals: Microinjection, Retrovirus mediated gene delivery, Embryonic stem cell mediated gene transfer; Knockout model systems & their utility; Animal as bioreactor.

 

Module-V (Assisted reproduction biotechnology for livestock improvement).

THEORY -Reproduction biotechnologies and their use in livestock; Somatic cell nuclear transfer cloning; In Vitro Fertilization, Embryo production, preservation and transfer; Sperm and embryo sexing; Intracytoplasmic sperm injection (ICSI); Cryopreservation and gamete banking.

 

Module-VI (Animal Production technology for sustainable agriculture and food security).

THEORY -Polyculture of fish for high yield; Edible oyster production; Pearl oyster production; Vermi-culture and vermicomposting for alternative and sustainable agriculture; Fish culture in flow through system and recirculation technology.

PRACTICE 1 - Vermicompost preparation from plant debris, cattle dung and paper waste.

 

Module-VII (Animal Biotechnology & human health).

THEORY - Recombinant therapeutics and production of pharmaceuticals; Production of tissues and organs for humans and xenotransplantation; Process of gene therapy, Pros and cons in gene therapy; Retrovirus and adenovirus mediated gene therapy.

PRACTICE 1 - Preparation of competent cell (Calcium chloride treatment method).

 

Text Books:

  • Freshney RI (1992) Animal cell culture: a practical approach, Oxford University Press.
  • Singh B, Gautam SK (2013) Text Book of Animal Biotechnology, TERI.

 

Reference Books:

  • SinghB, Mal G, Gautam SK, Mukesh M (2019) Advances in Animal Biotechnology, Springer
  • Butler M (2003) Animal Cell Culture and Technology, Taylor & Francis

 

 

Session Plan

 

Session Plan 1

Overview of animal cell and tissue culture technology

Slide Share_Overview of animal Biotech

 

Session Plan 2

Cell culture laboratory design and basic laboratory equipment

Slide Share_Basic Lab Equipment

Cell Culture Basics

 

Session Plan 3

Common laboratory hazards and Safety issues to consider in cell culture laboratory.

Slide Share_Common Lab Hazards

Lab safety and precautions

 

Session Plan 4

Summarization of Module-I (Doubt Clearance)

 

Session Plan 5 - Practice 1

Sterilization techniques used in animal cell culture

 

Session Plan 6 - Practice 2

Preparation of media for animal cell culture

 

Session Plan 7

Types of animal cell culture

Animal Cell Culture_Mod I and II

Slide Share_Types of animal cell culture

 

Session Plan 8

Characterization and preservation of animal cells

Slide Share_Preservation of cells

 

Session Plan 9

Opportunities and challenges in animal cell and tissue culture technology

Challenges during animal cell culture

 

Session Plan 10

Study of primary cell culture technique using chick embryo

PPT Materials_Chick embryo liver cell culture

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