Animal Biotechnology

Teacher

Sujit Kumar Mishra

Category

Core Courses

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Course Name : Animal Biotechnology

Code(Credit) : CUTM1452 (3-1-0)

Course Objectives

  • To make the student understand the tools and techniques required for the animal cell cultures, assisted reproductive technology, development of transgenic animals, and development of animal models.

Learning Outcomes

The students will learn

  • the methods of culturing animal cells

  • various techniques involve in making of  transgenic animals

  • to integrate assisted reproductive biotechnology techniques in livestock improvement.

  • to utilize animal production technologies for sustainable agriculture and food security

Course Syllabus

Module-I (Animal Cell and tissue culture technology-I): 8 Hrs

 

Overview of animal cell and tissue culture technology; Cell culture laboratory design and basic laboratory equipments; Media preparation, Role of important components of culture media; Common laboratory hazards and safety issues to consider in cell culture laboratory

 

Practice 1: Sterilization techniques used in animal cell culture

Practice 2: Preparation of media for animal cell culture

 

Module-II (Animal Cell and tissue culture technology-II): 8 Hrs

 

Types of animal cell culture; Characterization and preservation of animal cells; Advances in cell culture technology; Opportunities and challenges in animal cell and tissue culture technology

 

Practice 3: Study of primary cell culture technique using chick embryo

Practice 4: Animal cell batch culture technique

 

Module-III (Transgenesis for livestock improvement-I): 4 Hrs

 

Overview of transgenic technology; Biopharming through animal transgenesis; Methods of producing transgenic farm animals; Identification and transfer of gene influencing better production and disease resistance

 

 

Module-IV (Transgenesis for livestock improvement-II): 5 Hrs

 

Gene transfer methods in animals: Microinjection, Retrovirus mediated gene delivery, Embryonic stem cell mediated gene transfer; Knockout model systems & their utility; Animal as bioreactor

 

Module-V (Assisted reproduction biotechnology for livestock improvement): 4 Hrs

 

Reproduction biotechnologies and their use in livestock; Somatic cell nuclear transfer cloning; In Vitro Fertilization, Embryo production, preservation and transfer; Sperm and embryo sexing; Intracytoplasmic sperm injection (ICSI); Cryopreservation and gamete banking

 

Module-VI (Animal Production technology for sustainable agriculture and food security): 7 Hrs

 

Polyculture of fish for high yield; Edible oyster production; Pearl oyster production; Vermi-culture and vermicomposting for alternative and sustainable agriculture; Fish culture in flow through system and recirculation technology

 

Practice 5: Vermicompost preparation from plant debris, cattle dung and paper waste

 

Module-VII (Animal Biotechnology & human health): 6 Hrs

 

Recombinant therapeutics and production of pharmaceuticals; Production of tissues and organs for humans and xenotransplantation; Process of gene therapy, Pros and cons in gene therapy; Retrovirus and adenovirus mediated gene therapy

 

Practice 6: Preparation of competent cell (Calcium chloride treatment method)

 

Text Books:

  1. Freshney RI (1992) Animal cell culture: a practical approach, Oxford University Press

  2. Singh B, Gautam SK (2013) Text Book of Animal Biotechnology, TERI

 

Reference Books:

  1. Singh B, Mal G, Gautam SK, Mukesh M (2019) Advances in Animal Biotechnology, Springer

  2. Butler M (2003) Animal Cell Culture and Technology, Taylor & Francis

Session Plan

Session 1

Overview of animal cell and tissue culture technology

Video link: https://www.youtube.com/watch?v=RpDke-Sadzo

PPT link: https://www.slideshare.net/nagendragowda143/animal-cell-culture-techniques

Session 2

Cell culture laboratory design and basic laboratory equipments

Video link: https://www.youtube.com/watch?v=CA5fVLK5zAE

PPT link: https://www.slideshare.net/SubhalaxmiSwain1/equipments-used-in-animal-cell-culture

Session 3

Media preparation, Role of important components of culture media

Video: https://www.youtube.com/watch?v=cneascR3OEc

PPT: https://www.slideshare.net/RIZWANABBAS3/animal-cell-tissue-culture

Session 5 (Practice 1: 2 Hrs)

Sterilization techniques used in animal cell culture

https://www.youtube.com/watch?v=ugcGo42VNqI

Session 6 (Practice 2: 2 Hrs)

Preparation of media for animal cell culture

https://www.youtube.com/watch?v=RuRgCRASzpo&t=67s

Session 10

Opportunities and challenges in animal cell and tissue culture technology

Video: https://www.youtube.com/watch?v=w936wbss-5A

 

Session 11 (Practice 3: 2 Hrs)

Study of primary cell culture technique using chick embryo

https://www.youtube.com/watch?v=jA9DKdx04Xg

Session 12 (Practice 4: 2 Hrs)

Animal cell batch culture technique

https://www.youtube.com/watch?v=7d_kDu-P964

Session 16

Identification and transfer of gene influencing better production and disease resistance

Video: https://www.youtube.com/watch?v=ONs9FCY74p0

Session 17

Gene transfer methods in animals: Microinjection

Video: https://www.youtube.com/watch?v=R2OVe1qx444

Session 18

Gene transfer methods in animals: Retrovirus mediated gene transfer

Video: https://www.youtube.com/watch?v=G_NU3yXmmyI

PPT: https://www.slideshare.net/maryamshakeel792/retro-48281175

Session 19

Gene transfer methods in animals: Embryonic stem cell mediated gene transfer

Video: https://www.youtube.com/watch?v=CSEToZEhpo8

PPT: https://www.slideshare.net/DhavalChaudhary7/transgenesis-in-animals

Session 23

In vitro Fertilization, Embryo production, preservation and transfer

Video: https://www.youtube.com/watch?v=uXsCngh89fI&t=150s

PPT: https://www.slideshare.net/onlyuforu3/in-vitro-fertilization-37307905

Session 26

Polyculture of fish for high yield

Web link: http://www.fao.org/3/AC375E/AC375E03.htm

Session 29

Vermi-culture and vermicomposting for alternative sustainable agriculture

Video: https://www.youtube.com/watch?v=Ujy7CKNsWzk

PPT: https://www.slideshare.net/krishnaSethi1/vermicomposting-118274903

Session 30

Fish culture in flow through system and recirculation technology

Video: https://www.youtube.com/watch?v=W6lDgj5YwZg&t=43s

PPT: https://www.slideshare.net/sush_p/recirculation-in-fish

Session 31 (Practice 5: 2 Hrs)

Vermicompost preparation from plant debris, vegetable garbage, cattle dung and paper waste

Video: https://www.youtube.com/watch?v=HySDWOdWFoQ

Session 32

Recombinant therapeutics and production of pharmaceuticals

Video: https://www.youtube.com/watch?v=CJR33bu5zFE

PPT: https://www.slideshare.net/berran/recombinant-therapeutic-proteins

Session 33

Production of tissues and organs for humans and xenotransplantation

Video: https://www.youtube.com/watch?v=rq5k3da_UWk

PPT: https://www.slideshare.net/khehkesha/xenotransplantation-16315599

Session 34

Process of gene therapy, Pros and cons in gene therapy

Video: https://www.youtube.com/watch?v=xOQFJJOBGM0

PPT: https://www.slideshare.net/anniemirza14/gene-therapy-58257727

Session 36 (Practice 6: 2 Hrs)

Preparation of competent cell (Calcium chloride treatment method)

Vlab: http://vlab.amrita.edu/?sub=3&brch=77&sim=702&cnt=1

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    Sujit Kumar Mishra is working as Assistant Professor in the School of Applied Sciences, Centurion University of Technology and Management, Bolangir Campus, Odisha, India. He has completed his M. Sc. from School of Life Sciences, Sambalpur University, submitted his Ph. D. thesis in Sambalpur University, and awaiting award of the doctoral degree. He has awarded […]