Applied Microbiology
Teacher
Dr. Monali Priyadarsini Mishra
Category
Core Courses
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Course Name : CUTM1717-Applied microbiology
Code(Credit) : (2-1-0)
Course Objectives
- To impart knowledge of the basic principles of bacteriology, virology, including the nature of pathogenic microorganisms, pathogenesis, laboratory diagnosis, transmission, prevention and control of diseases common in the country
Course Outcomes
| COs | Course Outcomes | Mapping COs with POs (High-3, Medium-2, Low- 1) |
| CO1 | Know the applications of microbiology in diagnostics, hospitals and community | PO1(3), PO5(2) |
| CO2 | Learn principles underlying diagnostic tests and handle kits for diagnosis of diseases | PO2(2), PO3(3) |
| CO3 | Understand the prognosis of diseases and become aware of the role of medical microbiology in public health. | PO5(1) |
Course Syllabus
Module-1
The normal flora, collection and transport of clinical specimens, Collection and preliminary processing of specimens.
Practice: Isolation of Streptococcus mutans from oral cavity
Procedure for sample collection from skin, ear.
Module -2
Diagnostic microbiology- an approach to laboratory diagnosis, Rapid and automation methods in diagnostic microbiology, Molecular techniques in microbiology, Serological and skin tests
Practice: Demonstration of antigen-antibody reaction
Pus culture and sensitivity
Module -3
Microbiology in the service of human being, Community microbiology, Emerging and re-emerging Microbial disease, Nosocomial infections
Practice: Urine culture and sensitivity
Isolation of microorganism from spoiled fruit juice
Module -4
Hospital and laboratory waste, Diagnostic virology, Emergency microbiology Bacteriology of Milk, Air and Water
Practice: Isolation of microorganism from curd
Isolation of microorganism from Air
Suggested References
- Medical Parasitology by RL Ichhpujani and Rajesh Bhatia, jaypeepublisher
- Short text book of medical microbiology by Satish gupt, PublisherJaypee
Session Plan
Session 1
The normal flora
Video: https://www.youtube.com/watch?v=JYRkXhT1XEs
Source: The short text book of medical microbiology Pg-409
Session 2
The normal flora
Presentation: https://www.slideshare.net/FtoonMatuni/normal-flora-73259320
Session 3
collection and transport of clinical specimens
Video: https://www.youtube.com/watch?v=cIddzUYzUI4
Video: https://www.youtube.com/watch?v=C7W1WdXq-fI
Source: The short text book of medical microbiology Pg-411
https://www.cdc.gov/meningitis/lab-manual/chpt05-collect-transport-specimens.pdf
Session 4
collection and transport of clinical specimens
Source: https://www.palomarhealth.org/media/file/Lab/SpecimenCollectionHandling%20Manual.pdf
Session 5
Collection and preliminary processing of specimens
Video: https://www.youtube.com/watch?v=XpnqWdzxyBc
Presentation: https://www.slideshare.net/jit12/collection-and-transport
Session 6
Collection and preliminary processing of specimens
Source: The short text book of medical microbiology Pg-413; https://www.palomarhealth.org/media/file/Lab/SpecimenCollectionHandling%20Manual.pdf
Practice:
Isolation of Streptococcus mutans from oral cavity
Practice:
Procedure for sample collection from skin, ear
Session 7
Diagnostic microbiology- an approach to laboratory diagnosis
Session 8
Diagnostic microbiology- an approach to laboratory diagnosis
Source: The short text book of medical microbiology Pg-415
Presentation: https://www.slideshare.net/DCROWN/diagnostic-microbiology-35036899
Session 9
Rapid and automation methods in diagnostic microbiology
Source: The short text book of medical microbiology Pg-427
Session 10
Rapid and automation methods in diagnostic microbiology
Video: https://www.youtube.com/watch?v=io3gsbXTwIQ
Video: https://www.youtube.com/watch?v=DImEboaPNOE
Presentation: https://www.slideshare.net/farisk2/automated-methods-in-the-microbiology-lab-55370936
Session 11
Molecular techniques in microbiology
Video: https://www.youtube.com/watch?v=bRphJJO3Vy4
Presentation: https://www.slideshare.net/doctorrao/molecular-methods-in-the-diagnostic-microbiology
Session 12
Molecular techniques in microbiology
Source: The short text book of medical microbiology Pg-429
Session 13
Serological and skin tests
Source: The short text book of medical microbiology Pg-431
Session 14
Serological and skin tests
Presentation: https://www.slideshare.net/dream10f/serological-tests
Presentation: https://www.slideshare.net/GbolahanOlajuyigbe/allergy-skin-test
Session 15
Serological and skin tests
Video: https://www.youtube.com/watch?v=Oy5uixdzJ_c
Video: https://www.youtube.com/watch?v=LtqKB-qpJrs
Video: https://www.youtube.com/watch?v=nSjbNN-v-yA
Practice:
Demonstration of antigen-antibody reaction
Session 16
Microbiology in the service of human being
Source: The short text book of medical microbiology Pg-434
https://www.slideshare.net/doctortvrao/diagnostic-value-of-medical-microbiology
Session 17
Community microbiology
Source: The short text book of medical microbiology Pg-436
Session 18
Emerging and re-emerging Microbial disease
video: https://www.youtube.com/watch?v=D6bktJL8LCA
Video: https://www.youtube.com/watch?v=zJcSv8iOugk
Presentation: https://www.slideshare.net/arijitkundu88/emerging-and-reemerging-infectious-diseases-86061602
Session 19
Practice:
Isolation of microorganism from spoiled fruit juice
Session 20
Hospital and laboratory waste
Video: https://www.youtube.com/watch?v=iaDBE4-OlJs
Video: https://www.youtube.com/watch?v=irzeUovTmBc
Presentation: https://www.slideshare.net/sharrygajuryalnepal/hospital-waste-management-30528327
Session 21
Diagnostic virology
Video: https://www.youtube.com/watch?v=lVNbgDXBIM0
Video: https://www.youtube.com/watch?v=6us_DkrzzpQ
Presentation: https://www.slideshare.net/doctorrao/diagnosis-of-viral-infections-basics
Session 22
Emergency microbiology
Source:The short text book of medical microbiology Pg-449
Session 23
Bacteriology of Milk
Video: https://www.youtube.com/watch?v=1qXGrCs21rk
Presentation: https://www.slideshare.net/YNarayudu/analysis-of-milk
Video: https://www.youtube.com/watch?v=4hi6GYxhPNQ
Presentation: https://www.slideshare.net/gladysjanefranken/milk-microbiology
Session 24
Bacteriology of Air
Video: https://www.youtube.com/watch?v=Na3fJoz2Ytg&t=36s
https://www.youtube.com/watch?v=s1sKkwWA7zM
Presentation: http://www.brainkart.com/article/Bacteriology-of-Air_18628/
Video: https://www.youtube.com/watch?v=Na3fJoz2Ytg&t=36s
https://www.youtube.com/watch?v=s1sKkwWA7zM
Presentation: http://www.brainkart.com/article/Bacteriology-of-Air_18628/
Session 25
Bacteriology of Water
Video: https://www.youtube.com/watch?v=lzaAmeXKlqA
Video: https://www.youtube.com/watch?v=qoSvbbId18o
Presentation: https://www.slideshare.net/mirzafarullah/water-sampling-procedure-purpose-techniques-and-equipments
Video: https://www.youtube.com/watch?v=i0433lTS908
Presentation: https://www.slideshare.net/prakashtu/bacteriological-analysis-of-drinking-water-by-mpn-method
Video: https://www.youtube.com/watch?v=B1AVxzccS5Q
Video: https://www.youtube.com/watch?v=LpDjGCsmxZI
Presentation: https://www.slideshare.net/MariyaRaju/bacteriological-analysis-of-drinking-water
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC543678&blobtype=pdf
Case Studies
Case Studies
Our Main Teachers
Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]