Applied Microbiology

Teacher

Dr. Monali Priyadarsini Mishra

Category

Core Courses

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Course Name : CUTM1717-Applied microbiology

Code(Credit) : (2-1-0)

Course Objectives

  • To impart knowledge of the basic principles of bacteriology, virology, including the nature of pathogenic microorganisms, pathogenesis, laboratory diagnosis, transmission, prevention and control of diseases common in the country

Learning Outcomes

  • To know the applications of microbiology in diagnostics, hospitals and community

Course Syllabus

Module-1

The normal flora, collection and transport of clinical specimens, Collection and preliminary processing of specimens.

Practice: Isolation of Streptococcus mutans from oral cavity

                Procedure for sample collection from skin, ear.

Module -2 

Diagnostic microbiology- an approach to laboratory diagnosis, Rapid and automation methods in diagnostic microbiology, Molecular techniques in microbiology, Serological and skin tests

Practice: Demonstration of antigen-antibody reaction

                 Pus culture and sensitivity

Module -3

Microbiology in the service of human being, Community microbiology, Emerging and re-emerging Microbial disease, Nosocomial infections

Practice: Urine culture and sensitivity

                 Isolation of microorganism from spoiled fruit juice

Module -4 

Hospital and laboratory waste, Diagnostic virology, Emergency microbiology Bacteriology of Milk, Air and Water

Practice: Isolation of microorganism from curd

                  Isolation of microorganism from Air

Suggested Reading

  1. Medical Parasitology by RL Ichhpujani and Rajesh Bhatia, jaypeepublisher
  2. Short text book of medical microbiology by Satish gupt, PublisherJaypee

Session Plan

Session 1

The normal flora

Video: https://www.youtube.com/watch?v=JYRkXhT1XEs

Source: The short text book of medical microbiology Pg-409

Session 2

Session 3

collection and transport of  clinical specimens

Video: https://www.youtube.com/watch?v=cIddzUYzUI4

Video: https://www.youtube.com/watch?v=C7W1WdXq-fI

Source: The short text book of medical microbiology Pg-411

https://www.cdc.gov/meningitis/lab-manual/chpt05-collect-transport-specimens.pdf

Session 4

collection and transport of  clinical specimens

Source: https://www.palomarhealth.org/media/file/Lab/SpecimenCollectionHandling%20Manual.pdf

Session 5

Collection and preliminary processing of specimens

Video: https://www.youtube.com/watch?v=XpnqWdzxyBc

Presentation: https://www.slideshare.net/jit12/collection-and-transport

Session 6

Collection and preliminary processing of specimens

Source: The short text book of medical microbiology Pg-413; https://www.palomarhealth.org/media/file/Lab/SpecimenCollectionHandling%20Manual.pdf

Practice:

Isolation of Streptococcus mutans from oral cavity

Video: https://www.youtube.com/watch?v=3zL4Hou1P-c

Video: https://www.youtube.com/watch?v=yC4EJeGcyVI

Practice:

Procedure for sample collection from skin, ear

Video: https://www.youtube.com/watch?v=Zd9IvCY3DY8

Video:https://www.youtube.com/watch?v=GV840R4Pyi4

Session 7

Diagnostic microbiology- an approach to laboratory diagnosis

Video: https://www.youtube.com/watch?v=0_GuT_jqoxo

Session 8

Diagnostic microbiology- an approach to laboratory diagnosis

Source: The short text book of medical microbiology Pg-415

Presentation: https://www.slideshare.net/DCROWN/diagnostic-microbiology-35036899

Session 9

Rapid and automation methods in diagnostic microbiology

Source: The short text book of medical microbiology Pg-427

Video: https://www.youtube.com/watch?v=cMVyrrdgaYk

Session 10

Session 11

Session 12

Molecular techniques in microbiology

Source: The short text book of medical microbiology Pg-429

Video: https://www.youtube.com/watch?v=cMVyrrdgaYk

Session 13

Serological and skin tests

Source: The short text book of medical microbiology Pg-431

Video: https://www.youtube.com/watch?v=wGZU1-HfsR4

Session 14

Session 15

Practice:

Demonstration of antigen-antibody reaction

Video: https://www.youtube.com/watch?v=pC9EtQ8fWbE

Practice:

Pus culture and sensitivity

Video: https://www.youtube.com/watch?v=qYWnfJi1f0c

Session 16

Microbiology in the service of human being

Source: The short text book of medical microbiology Pg-434

https://www.slideshare.net/doctortvrao/diagnostic-value-of-medical-microbiology

https://www.slideshare.net/SamiptaSingh/microbiology-ppt

Session 17

Community microbiology

Source: The short text book of medical microbiology Pg-436

Session 18

Session 19

Practice:

Urine culture and sensitivity

Video: https://www.youtube.com/watch?v=JkxKvvSkZ_k

Practice:

Isolation of microorganism from spoiled fruit juice

Video: https://www.youtube.com/watch?v=pvCuPPsyb4E

Session 20

Session 21

Session 22

Emergency microbiology

Source:The short text book of medical microbiology Pg-449

Session 23

Session 24

Session 25

Practice:

Isolation of microorganism from curd

Video: https://www.youtube.com/watch?v=0yX2D7nfa7M

Practice:

Isolation of microorganism from Air

Video: https://www.youtube.com/watch?v=Na3fJoz2Ytg

Case Studies

Case Studies

Our Main Teachers

Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]