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Module-I Chromatography: General Principles of chromatography, adsorption and partition. Techniques: Paper chromatography, Ascending, descending and circular, 2D chromatography, Rf values, column chromatography.
Module-II Principle of gel filtration, ion-exchange chromatography, affinity chromatography.
Thin layer chromatography (TLC) – Principle, procedure and applications.
Module-III Electrophoresis, Paper and gel electrophoresis, SDS-PAGE, 2D electrophoresis.
Module-IV Centrifugation: Principle of differential centrifugation. Types of Rotors, Ultra centrifuge –construction and applications in sub-cellular fractionation.
Module-V Radiochemistry: Natural and artificial radioactivity, characteristics of radioactive elements, units of radioactivity, disintegration constant, half-life, α, β and γ radiation. Detection of radioactivity by GM counter. Applications of radioisotopes – 3H, 14C, 131I, 60Co and 32P. Biological effects of radiations. Safety measure in handling radio isotopes.
Module-VI Types of ELISA, principle and application of ELISA,
Module-VII Spectrometry–Beer and Lamberts law, Principals of colorimeter, spectrophotometer. Nephelometry and turbidimetry and its applications
1. Identification of amino acids by paper chromatography.
2. Ascending paper chromatography of amino acids.
3. Separation of plant pigments using paper chromatography.
4. Separation of plant pigments by column chromatography using silica gel-G.
5. Polyacrylamide gel electrophoresis of proteins.
7. Demonstration of separation of lipids by TLC.
8. Demonstration of two dimensional chromatography of amino acids
Practical Biochemistry – Principles and Techniques, Wilson and Walker, Cambridge, University Press, Cambridge
Biophysical Chemistry, Principles & Techniques – Upadhyay, Upadhyay and Nath Himalaya Publ. House.
Practical Biochemistry – Principles and Techniques, Wilson and Walker, Cambridge University Press, Cambridge
Boyer, R.F., Biochemistry Laboratory: Modern Theory and Techniques, 6th Ed., Boston, Mass: Prentice Hall, 2012.
Plummer D. T., An Introduction to Practical Biochemistry 3rd ed., Tata McGraw
Hill Education Pvt. Ltd. (New Delhi), 1998.
Dr Sitaram Swain has received PhD degree from Utkal University in the year 2017. He is also trained and experienced from TATA Memorial Hospital, Mumbai, India. He has experienced with cell line culture, clinical biochemistry, immuno-fixation, immunoassay, cancer cytogenetic, serum protein electrophoresis and tumour marker analysis. He has qualified NET(ICAR-ASRB) in Animal biotechnology. He has […]