Biochemistry and Enzyme Technology
Teacher
Dr. Shantanu Bhattacharyya
Category
Core Courses
Course Attendees
Still no participant
Course Reviews
Still no reviews
Course Name : Biochemistry and Enzyme Technology
Code(Credit) : CUTM1433(3-1-0)
Course Objectives
This course provides
- The theory and knowledge relevant to the plant Biochemistry and enzymology principles
- Fundamental properties of enzymes, enzyme catalytic mechanisms and enzyme kinetics.
- Techniques employed in enzymes purification and characterization are also emphasized in this course.
- Students will also be introduced to the theory as well as applications of enzyme technology in food, medical, and household industries.
- this course serves to provide an awareness of the current and possible future applications of enzyme technologies
Course Outcomes
| COs | Course outcomes | Mapping COs with POs (High-3, Medium-2, Low-1) |
| CO1 | Gain comprehensive theoretical knowledge on the kingdoms of biomolecules, bioenergetics principals that are the prerequisites and consequences of physiological phenomenon for further manipulations. | PO1-3, PO2-2,PO4-3,PO5-2 |
| CO2 | Learn the purification, characterization and estimation of enzymes. Apply biochemical calculation for enzyme kinetics and can plot graphs based on kinetics data | PO1-3, PO2-3,PO4-3,PO5-3 |
Course Syllabus
Module-I Essential Biochemistry and Bioenergetics
1.1 Structure of atoms and molecules, chemical bonds.
1.2 Stabilizing Interactions (thermodynamics of folding, conformational entropy, charge charge interaction, Vander wall force, hydrophobic effect, disulfide bonds, prosthetic group, ion binding protein stability),
1.3 pH, buffer, Acid Base Equilibria, Water.
1.4 Biological Thermodynamics, Enthalpy and Entropy, Standard Free Energy Concept and Calculation, Biological Energy transducer Cellular energy currency, Energy rich compounds
Practice: Demonstration of weak interaction on stability of protein
Module-II Biochemistry of Protein
2.1 Building Block of Proteins: Chemical Properties of Proteins, common plant Protein Sources, Protein Databases.
2.2 Amino acids (genetically coded, Rare genetically coded, modified), Dissociation constant, Isoelectric point, Assay of Amino acids, protein denaturation and renaturation; Prions
2.3 Structural Organization: Structural organization of Protein (different models), Dynamics of Protein Structure (globular, Fibrous), chaperon concept
2.4 Ramachandran Plot. Specialized Secondary Structure protein structure (TMV, Hemoglobin, Myoglobin, collagen, Carboxypeptidase, RuBisCo)
2.5 Domain and Motifs: Motifs, domains, Models, Functional relationship between domains and function of proteins, super secondary structures of proteins Classification of proteins based on the structures like Zn finger, lucine zipper proteins etc
Practice:
Quantitative estimation of proteins Biuret, Lowry, BCA and Bradford methods
Quantitative estimation of amino acids
Module-III Biochemistry of Nucleic Acid, Carbohydrates,
3.1 Nucleic Acid: Structure, Models and Stability of Nucleic Acids (DNA/RNA), Nucleotide Databases,
3.2 Primary and Secondary Structure, Alternate Secondary Structure: Hairpin, Cruciform, triple Helix, G-quadrates etc. Denaturation, Tm value, Protein DNA interaction
3.3 Carbohydrate: Structure types and Nomenclature, Structure Function relationship Carbohydrate Databases.
Practice:
Quantitative estimation of Carbohydrates
Module-IV Biochemistry of Lipid, Vitamins, and Antioxidants
4.1 Lipid: classification, structure, properties and functions of fatty acid, essential fatty acids, fats, phospholipids, sphingolipids, cerebrosides, steroids, lipoproteins, membrane protein. Plant Biofuel
4.2 Vitamins and Antioxidants: Structure and functions of vitamins, Source of phytovitamins, Natural Antioxidants
Practice:
Determination of Unknown Concentration of Vitamin C
Module-V Enzymology
5.1 Overview of enzyme: Chemical Nature, Characteristics, Classification (IUB), Nomenclature,
5.2 Enzyme conformation, Active Sites, Ribozyme, isozymes, multi enzyme complex.
5.3. Enzyme kinetics: Michelis Menten equation, Treatment of Data (Briggs-Haldane, lineweaver-burk, Eadie-hofstee, cornish-bowden, Van Slyke-Cullen behavior) Enzyme inhibitiion, Significance of Km. Kcat,Vm
5.4 Single substrate, bi substrate, multi substrate reaction, Significance and Evaluation of activation energy.
5.5 Mechanism of enzyme action: Enzyme catalytic reaction mechanism, chymotrypsin, lysozyme, Serine protease, Alcohol dehydrogenase, carboxypeptidase,
5.6 Co enzymes and its role in Enzyme action (NAD+/NADP+, FAD, lipoic acid, thiamine pyrophosphate, tetrahydrofolate, biotin, pyridoxal phosphate, B12 etc.)
5.7 Allosteric enzyme and its mechanism
5.8 Enzyme catalysis: Acid-base catalysis, covalent catalysis, metal ion catalysis, proximity, orientation effect, site directed mutagenesis of enzyme.
Practice:
Effects of pH, Temperature, and Substrate Concentration on enzyme activity
Detection of Active site of enzyme using computational tool
Module-VI Enzyme Technology
6.1 Enzyme Purification, Assay, Large Scale production of Enzyme
6.2 Enzyme Immobilization (kinetics), enzyme reactor,
6.3 Biotransformation, Nobel Enzyme,
6.4 Scope of enzyme technology in medicine, Detergents, Food and breverage industry, Leather Industry, Textile industry etc).
Practice:
Isolation and purification of enzyme/proteins
Module-VII Industrial Biochemistry
7.1 Industrial Enzymes – production & applications,
7.2 Biofuel: production of biomethane and bioethanol from agro-food wastes/Microalgae,
7.3 Nutritional profiling of agricultural products,
7.4 Biopolymer, Microbial polysaccharides. Dextrans. Polyhydroxy alkanoates. Polyhydroxybutyrate (PHB). Biodegradable plastics
Practice:
Bio-diesel production from Biomass and its Characterization
Text Book
-
Donald Voet, Judith G. Voet,Biochemistry, Wiley
-
David L. Nelson; Michael M. Cox,Lehninger Principles of Biochemistry, W.H freeman and Company
-
Satyanarayana, U. and Chakrapani, U,Biochemistry, Elsevier
-
Murray, Darryl K. Granner, Peter A. Mayes,Harper's Illustrated Biochemistry, Tata Mc Graw hill
Reference Book
-
Trevor Palmer, Philip L. Bonner - Enzymes_ Biochemistry, Biotechnology, Clinical Chemistry-Woodhead Publishing
-
Leskovacs V. Comprehensive enzyme kinetics, Kluwer
-
Lemuel Wingard, Applied Biochemistry and Bioengineering. Enzyme Technology, Elsevier Science
-
Robert_Rastall_Novel_Enzyme_Technology_for_Food, Woodhead Publishing
Session Plan
Session 1
Structure of atoms and molecules, chemical bonds.
https://www.youtube.com/watch?v=cV4jJZCIMPo
https://www.youtube.com/watch?v=OTgpN62ou24
Session 2
Stabilizing Interactions (thermodynamics of folding, conformational entropy, charge charge interaction, Vander wall force, hydrophobic effect, disulfide bonds, prosthetic group, ion binding protein stability),
Hydrogen Bond PDF ; Stabilizing interaction PDF
https://www.youtube.com/watch?v=ErQORXtdpEg
https://www.youtube.com/watch?v=AD7CoDwns7o
https://www.youtube.com/watch?v=HGc9RFD7iSE
https://lab.concord.org/embeddable.html#interactives/samples/3-100-atoms.json
Session 3: (Practice-1: 1hr)
Demonstration of weak interaction on stability of protein
https://lab.concord.org/embeddable.html#interactives/samples/3-100-atoms.json
Session 5
Biological Thermodynamics, Enthalpy and Entropy, Standard Free Energy Concept and Calculation, Biological Energy transducer Cellular energy currency, Energy rich compounds
energy currency PDF; Thermodynamic principle PDF
https://www.youtube.com/watch?v=P2WD9jFePwo
https://www.youtube.com/watch?v=DPjMPeU5OeM
https://www.youtube.com/watch?v=t6AwXiWyybk
Session 6
Building Block of Proteins: Chemical Properties of Proteins, common plant Protein Sources, Protein Databases.
peptides_proteinsPDF; Study material PDF; PPT
Session 7: (Practice-2: 2hr)
Quantitative estimation of proteins (Biuret/Lowry/ BCA/Bradford methods)
https://www.labome.com/method/Protein-Quantitation.html
Session 8
Amino acids (genetically coded, Rare genetically coded, modified), Dissociation constant, Isoelectric point, Assay of Amino acids, protein denaturation and renaturation; Prions
http://courseware.cutm.ac.in/wp-content/uploads/2020/06/amino-acid-ppt-1.pdf
Session 9: (Practice 3: 2hr)
Quantitative estimation of amino acids
Session 10
Structural Organization: Structural organization of Protein (different models), Dynamics of Protein Structure (globular, Fibrous), chaperon concept
https://www.youtube.com/watch?v=Pjt1Q2ZZVjA
https://www.youtube.com/watch?v=yZ2aY5lxEGE
https://www.youtube.com/watch?v=nEHe3Aie9Ek
Session 11
Ramachandran Plot, Specialized Secondary Structure protein structure (TMV, Hemoglobin, Myoglobin, collagen, Carboxypeptidase, RuBisCo)
https://www.youtube.com/watch?v=aO0l1PReGo0&t=5s
https://www.youtube.com/watch?v=Q1ftYq13XKk
Session 12
Domain and Motifs: Motifs, domains, Models, Functional relationship between domains and function of proteins, super secondary structures of proteins Classification of proteins based on the structures like Zn finger, lucine zipper proteins etc
https://www.youtube.com/watch?v=2-qFLfVymnw
Session 13
Nucleic Acid: Structure, Models and Stability of Nucleic Acids (DNA/RNA), Nucleotide Databases
Discovery of DNA Double Helix_ Watson and Crick _ Learn Science at Scitable
https://www.youtube.com/watch?v=V6bKn34nSbk&feature=youtu.be
Session 14
Primary and Secondary Structure of Nucleic Acid, Alternate Secondary Structure: Hairpin, Cruciform, triple Helix, G-quadrates etc. Denaturation, Tm value, Protein DNA interaction
Session 15
Carbohydrate: Structure types and Nomenclature, Structure Function relationship Carbohydrate Databases.
Session 16: (Practice-4 :2hr)
Quantitative estimation of Carbohydrates
Session 17
Lipid: classification, structure, properties and functions of fatty acid, essential fatty acids, fats, phospholipids, sphingolipids, cerebrosides, steroids, lipoproteins, membrane protein. Plant Biofuel
Session 18
Vitamins and Antioxidants: Structure and functions of vitamins, Source of phytovitamins, Natural Antioxidants
https://www.slideshare.net/ISFCPISFCollegeofPha/antioxidants-102691615
Session 19:(Practice-5 :2hr)
Determination of Unknown Concentration of Vitamin C
Session 20
Overview of enzyme: Chemical Nature, Characteristics, Classification (IUB), Nomenclature,
https://www.slideshare.net/PRITHWISHBARIK/enzyme
https://www.qmul.ac.uk/sbcs/iubmb/enzyme/index.html
https://www.qmul.ac.uk/sbcs/iubmb/enzyme/rules.html
https://www.youtube.com/watch?v=PNyvtcu5-EU
Session 21
Enzyme conformation, Active Sites, Ribozyme, isozymes, multi enzyme complex.
https://www.tuscany-diet.net/2019/08/16/multienzyme-complexes/
https://www.youtube.com/watch?v=wp_yyDEEC3k
Session 22 (Practice 6: 2hr)
Effects of pH, Temperature, and Substrate Concentration on enzyme activity
Session 23
Enzyme kinetics: Michelis Menten equation, Treatment of Data (Briggs-Haldane, lineweaver-burk, Eadie-hofstee, cornish-bowden, Van Slyke-Cullen behavior) Enzyme inhibitiion, Significance of Km. Kcat,Vm
https://www.youtube.com/watch?v=TTr2ADsjU10
https://www.youtube.com/watch?v=3VCZWPcaxJU
Session 24
Single substarte, bi substrate, multi substrate reaction, Significance and Evaluation of activation energy.
Session 25
Mechanism of enzyme action: Enzyme catalytic reaction mechanism, chymotrypsin, lysozyme, Serine protease, Alcohol dehydrogenase, carboxypeptidase,
https://www.youtube.com/watch?v=yk14dOOvwMk
Session 26
Co enzymes and its role in Enzyme action (NAD+/NADP+, FAD, lipoic acid, thiamine pyrophosphate, tetrahydrofolate, biotin, pyridoxal phosphate, B12 etc.)
https://www.slideshare.net/polly0007/co-enzymes
Session 28
Enzyme catalysis: Acid-base catalysis, covalent catalysis, metal ion catalysis, proximity, orientation effect, site directed mutagenesis of enzyme.
Session 29
Enzyme Purification, Assay, Large Scale production of Enzyme
https://www.slideshare.net/extraction-purification-and-production-of-enzymes
Session 30:(Practice-7: 2hr)
Isolation and purification of enzyme/proteins
Session 31
Enzyme Immobilization (kinetics), enzyme reactor,
https://www.slideshare.net/enzyme-immobization-and-applications
https://www.slideshare.net/immobilization-of-enzymes
Session 33
Scope of enzyme technology in medicine, Detergents, Food and breverage industry, Leather Industry, Textile industry etc).
https://www.slideshare.net/industrial-applications-of-enzymes
https://www.slideshare.net/enzymes-in-dairy-industry
https://www.youtube.com/watch?v=waqhbC0dLKU
Session 34
Industrial Enzymes – production & applications,
Session 35
Biofuel: production of biomethane and bioethanol from agro-food wastes/Microalgae,
https://www.slideshare.net/green-algae-as-a-biofuel
https://www.youtube.com/watch?v=QP_HbQ5cWSk
Session 36: (Practice-8: 2hr)
Biodiesel production from Biomass, and its characterization
https://www.jove.com/video/54189/experimental-protocol-for-biodiesel-production-with-isolation
Session 37
Biopolymer, Microbial polysaccharides. Dextrans. Polyhydroxy alkanoates. Polyhydroxybutyrate (PHB). Biodegradable plastics
https://www.slideshare.net/microbial-polysaccharide
https://www.slideshare.net/bioplastic
https://www.youtube.com/watch?v=hvbs_NzgfhI
https://www.youtube.com/watch?v=v2V4zMx33Mc&list=PL98qL1N8ZU3765EDxE77j4cCyDw_MN3Sm
Case Studies
Case Studies
Our Main Teachers
Dr. Shantanu Bhattacharyya
Assistant Professor, Department of Botany, School of Applied Science ( Bolangir Campus)
VIEW PROFILEDr. Shantanu Bhattacharyya works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha, India. He has received his M.Sc. (Life Science- Botany) and M.Phil (Life Sciences) from Sambalpur University, Orissa, India and B.Ed. from Singhania University, Rajasthan India. He qualified GATE-2007, CSIR-UGC NET LS -2008. He […]