Biochemistry and Metabolic Processes

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Sunita Satapathy

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Core Courses

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BSZO2303

 

Course Name : Biochemistry & Metabolic Processes

Code(Credit) : CUTM1506(3-2-1)

Course Objectives

  • To know the functioning of various organs and their inter relationship.
  •  To make students to know about the various metabolic processes

Learning Outcomes

  • Students will acquire the knowledge of functioning of different body parts.
  • The students can apply the acquired knowledge for higher study.

Course Syllabus

MODULE- I     

Overview of Metabolism :

 1.1- Stages of Metabolism (Catabolism vs Anabolism)

 1.2-  Compartmentalization of metabolic pathways,

 1.3-ATP as "Energy Currency of cell" &  Use of reducing equivalents and cofactors

 1.4-Intermediary metabolism and regulatory mechanisms

Practice-1

To demonstrate the effect of temperature on enzyme activity.

Assignment-1-a) Intermediary metabolism and regulatory mechanisms

 

MODULE- II

Carbohydrate Metabolism :

 2.1-Sequence of reactions and regulation of glycolysis & Gluconeogenesis

 2.2-Citric acid cycle and significance

 2.3-Phosphate Pentose Pathway (Role and significance)

2.4-Glycogenesis

 2.5-Glycogenolysis

Practice-2

To estimate the carbohydrate content of supplied tissue by Spectrophotometry method

Practice-3

To estimate the glycogen content of supplied tissue by colorimeter method

Assignment-2 -a) Significance of Glycolysis, b) Role of Phosphate Pentose Pathway, c) Glycogenesis-role of UDP-Glucose-PPT

MODULE- III 

3. Lipid Metabolism:

3.1-β-oxidation of saturated fatty acids with even & odd number of carbon atoms

3.2- Omega -oxidation of even & odd number of saturated fatty acids

3.3-Biosynthesis of palmitic acid

3.4-Metaboliosm of unsaturated fatty acid

3.5-Ketogensis

3.6-Metabolic Fuel Utilization & Regulation of fatty acid

 

Practice-4

To estimate the lipid content from the given sample

Practice-5

Dry Lab: To trace the labelled C atoms of Acetyl-CoA till in the TCA cycle

Assignment-3  -a) Beta -Oxidation, b)Metaboliosm of unsaturated fatty acid

 

MODULE- IV 

Protein Metabolism:

4.1-Catabolism of amino acids

4.2-Transamination

4.3-Deamination

4.4-Protein Metabolidsm

Practice-5

To estimate the protein content of supplied tissue by colorimeter method

Practice-6

Estimation of total protein content in the given sample by Lowry’s /Barfoed's Method.

Practice-7

To estimate  amino acids from a mixture by using chromatography (paper/Thin layer)

Assignment-4- a) Role of aminotransferease, b)Protein Metabolism-PPT

 

MODULE- V 

Fate of carbon skeleton

5.1-Urea cycle

5.2-Fate of C-skeleton of Glucogenic & Ketogenic amino acids

5.3-Regulation of Urea cycle

5.4-Role of mitochondria

Practice-8

Detection of SGOT in given sample using available kit. (Chick/Goat Serum

Practice-9

Determination of SGPT in given sample by using available kit.(Chick/Goat Serum)

Assignment-5  a) Role of mitochondria in urea cycle, b) Role of enzymes of urea cycle

 

MODULE- VI 

Oxidative Phosphorylation 

6.1-Oxidative Phosphorylation

6.2-Redox systems & Coupled reactions

6.3-Review of mitochondrial respiratory chain

6.4-Shuttle systems & Membrane transporters

 

Practice-10

Estimation of known and unknown protein

Practice-11

Preparation of Standard Curve of Bovine serum Albumin

Assignment-6 a) Role of ETS ,b) Shuttle system-PPT/e-assigned note

MODULE- VII 

 Inhibitors and Uncouplers

7.1-Inhibitors  of Electron Transport System

7.2-Uncouplers  of Electron Transport System

7.3-Activity of Enzyme

 

Practice-12

Study the enzymatic activity of Trypsin and Lipase

Session Plan

Session-1

1.1- Stages of Metabolism (Catabolism vs Anabolism)

Overview on metabolism

Overview on metabolism-1

Session 2

 1.2-Compartmentalization of metabolic pathways

compartmentalization

compartmentalization of cell

compartmentalization-p

Session 3

 1.3-ATP as "Energy  Currency of cell" and Use of reducing equivalents and cofactors,Couple reaction

ATP- currency of energy-1

ATP-currency of energy-2

Reducing equivalent

Couple reaction-Inhibitor

Session 4

 1.4-Intermediary metabolism and regulatory mechanisms

Intermediary metabolism

Regulation of metabolism

Overview on metabolism

Session 5

Practice-1 -To demonstrate the effect of temperature on enzyme activity

Estimation of enzyme activity-1

Session 6

Assignment-1- a)Types of molecules involve in metabolism, b)Notes on ATP, c)Intermediary reactions

Session 7

Flip class 1- Role of ATP on metabolic reaction

ATP- currency of energy

Session-8

2.1 -Sequence of reactions and regulation of Glycolysis & Gluconeogenesis

Glycolysis-1

Glycolysis-2

Glycolysis-3

Gluconeogenesis-1

Gluconeogenesis-2

Gluconeogenesis-3

Session 10

2.3-Phosphate pentose pathway

HMP-shunt-1

HMP-shunt-2

Session 11

2.4-Glycogenesis

Glycogenesis-1

Glycogenesis-2

Session 12

2.5-Glycogenolysis

Glycogenolysis-1

Glycogenolysis-2

Session 13

Practice-2

To estimate the carbohydrate from content of supplied tissue

estimation of carbohydrate 

Session 14

Practice-3

Qualitative  estimation of  carbohydrate from supplied sample.

Estimation of carbohydrate

Session 15

Assignment-2 -a) Significance of Glycolysis, b) Role of Phosphate Pentose Pathway, c) Glycogenesis-role of UDP-Glucose-PPT

Session 16

Session 17

3.1-β-oxidation of saturated fatty acids with even number of carbon atoms

Beta-oxidation-1

Beta-oxidation-2

Session 18

3.2-Omega -oxidation of saturated fatty acids with  even and odd number of carbon atoms

omega-oxidation-1

omega-oxidation-2

 

Session 19

Session 20

3.4-Metabolism of Unsaturated fatty acid

unsaturated fattyacied-yt

unsaturated fattyacied-

turated

Session 21

3.5-Ketogenesis

ketogenesis-yt

ketogenesis-yt

Session 22

3.6--Metabolic Fuel Utilization & Regulation of fatty acid

Metabolic Fuel Utilization

Metabolic Fuel Utilization

Session 23

Practice-4

To estimate the lipid content from the given sample

Estimation of  lipid

Lipid estimation-1

Lipid estimation-2

Session 24

Assignment-3-Dry run of Beta Oxidation of Fatty Acid-PPT/Programming

Session 25

Flip Class :3-Beta oxidation

Beta-oxidation

Session 27

Session 28

4.3-Deamination & it's regulation

Deamination-1

Deamination-2

Deamination-3

Session 29

4.4--Protein Metabolism

protein estimation

Session 30

Practice-5

To estimate the protein content of supplied tissue by colorimeter method

Estimation of protein

Session 31

Practice-6

Estimation of total protein content in the given sample by Lowry’s /Barfoed's Method.

PV-6-Estimation of protein

Session 32

Practice-7

To estimate  amino acids from a mixture by using chromatography (paper/Thin layer)

Paper-chromatography-1

Paper-chromatography=2

Session 33

Assignment-4- a) Role of aminotransferease, b)Protein Metabolism-PPT

Session 34

Flip Class -4-Protein metabolism

Paper-chromatography

Session 35

5.1-Urea cycle & it's importance

Urea cycle-1

urea-cycle-2

Urea-cycle3

Urea cycle-4

Session 36

5.2-Fate of C-skeleton of Glucogenic & Ketogenic amino acids

U-cycle-2

Session 40

Practice-9

Estimation of albumin in urea

Urea-cycle-Albumin in urea

Urea-albumin test

Session 41

Assignment-5  a) Role of mitochondria in urea cycle, b) Role of enzymes of urea cycle

Session 42

Session 43

6.2-Redox system & coupled reactions

Rdox-coupled raction-1

Redox-coupler-reaction-2

Session 44

6.3-Review of mitochondrial respiratory chain

Respiratory chain

Respiratory chain-1

Respiratorychain-2

Respiratorychain-3

Session 45

Session 46

Practice-10

Estimation of known and unknown protein

Protein estimation-10

Session 47

Practice-11

Preparation of Standard Curve of Bovine serum Albumin

Protein Estimation-11

Session 48

Assignment-6 a) Role of ETS ,b) Shuttle system-PPT/e-assigned note

Session 49

Topic-7.1-Inhibitors of Electron Transport System

Inhibitor& coupler-1

Inhibitor- coupler-2

UncouplerETS

Session -50

Topic-7.2-Enzyme activation

Enzyme activation-1

Enzyme activation-2

Session -51

7.3- Activity of Enzyme

Enzyme

Ewnzyme-work

Enzyme catalys

Session 52

Practice-12

Study the enzymatic activity of Trypsin and Lipase

Enzym Estimation

Enzyme estimation

Enzyme catalyst-test

  1. Reference

 

E-content:

 

Text Books:

  1. Cox, M.M and NelsoEXT n, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition, W.H. Freeman and Co., New York.

Reference Books:

  1. Cox, M.M and NelsoEXT n, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition,
  2. W.H. Freeman and Co., New York.
  3. Berg, J.M., Tymoczko, J.L. and Stryer, L. (2007). Biochemistry, VI Edition, W.H. Freeman and Co., New York.rs
  4. Sources of Environmental hazards, hazard identification and accounting, fate of toxic and persistent substances in the environment, dose Response Evaluation, exposure Assessment.

 

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    Sunita Satapathy

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    Experienced with teaching profession in the field of Biochemistry,Animal Physiology, Cell biology. Presently working as Assistant Professor in Department of Zoology,School of Applied Science,Centurion University of Technology & Management, Odisha, India. Pursuing P.hD in Vermitechnology (recycling Biodegradable Wastes ) in CUTM. Received M.Phil in Biotechnology on Activity of Enzyme and M.Sc. in BIOCHEMISTRY as Specialisation […]