BIOCHEMISTRY (Practical)
Teacher
Satyanarayan Tripathy
Category
Core Courses
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Course Name : Biochemistry(Practical)
Code(Credit) : BPHL1203(0-2-0)
Course Objectives
- Biochemistry deals with complete understanding of the molecular levels of the chemical process associated with living cells. The scope of the subject is providing biochemical facts and the principles to understand metabolism of nutrient molecules in physiological and pathological conditions. It is also emphasizing on genetic organization of mammalian genome and hetero & autocatalytic functions of DNA.
Course Outcomes
| CO | Statements | Mapping With POs |
| CO1 | describe the cell organization and can differentiate between the different transport mechanisms present across the cell membrane. | PO 1,PO 3 |
| CO2 | define and distinguish between different terms of carbohydrate metabolism and memorise the pathways or cycles and gather the different pathways knowledge about role of citric acid cycle | PO 1 |
| CO3 | describe various lipid oxidation processes and biosynthesis of fatty acids, and they acquire knowledge about disorder related to lipid metabolism and also explain the amino acid metabolism and disorder. | PO 1 |
| CO4 | describe nucleic acid and biosynthesis of nucleic acids, and they acquire knowledge about genetic information transfer. | PO 1 |
| CO5 | define enzymes, vitamins, co-enzymes and moreover, they can describe the factors affecting the enzyme action, classify different enzymes and explain the kinetics and MOA of enzymes. | PO 1,PO 3 |
Course Syllabus
- Qualitative analysis of carbohydrates (Glucose, Fructose, Lactose, Maltose, Sucrose and starch)
2. Identification tests for Proteins (albumin and Casein)
3. Quantitative analysis of reducing sugars (DNSA method) and Proteins (Biuret method)
4. Qualitative analysis of urine for abnormal constituents
5. Determination of blood creatinine
6. Determination of blood sugar
7. Determination of serum total cholesterol
8. Preparation of buffer solution and measurement of pH
9. Study of enzymatic hydrolysis of starch
10. Determination of Salivary amylase activity
11. Study the effect of Temperature on Salivary amylase activity.
12. Study the effect of substrate concentration on salivary amylase activity.
Session Plan
Session 1
Qualitative analysis of carbohydrates
Session 3
Quantitative Analysis of reducing sugars(DNSA method)
Session 4
Quantitative Analysis of protein(Biuret Method)
Session 5
Qualitative analysis of urine for abnormal constituents
Session 9
Preparation of buffer solution and management of PH
Session 12
Study the effect of Tempreture on Salivary amylase activity
Session 13
Study the effect of substrate concentration on salivary amylase activity
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