Biochemistry

Teacher

Dr. Soumya Jal

Category

Core Courses

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Course Name : Biochemistry

Code(Credit) : CUTM1732 (3-1-0)

Course Objectives

  • To understand the concept of metabolism of carbohydrates
  • To understand the significance of amino acids, proteins
  • Use of enzymes in enhancing metabolic reactions
  • Role of lipids

Learning Outcomes

  • After completion of the course the student will be developed a very good understanding of various biomolecules which are required for development and functioning of cells.
  • Would have understood the significance of carbohydrates in energy generation and as storage food molecules for cells.
  • They would have understood the significance of proteins and enzymes in accelerating various metabolic activities.
  • The conceptual understanding of the subject provides opportunities for skill enhancement and scopes for higher education.

Course Syllabus

Unit-1

Structure of enzyme: Apoenzyme and cofactors, prosthetic group-TPP, coenzyme NAD, metal cofactors, Classification of enzymes.

Mechanism of action of enzymes: active site, transition state complex and activation energy. Lock and key hypothesis, and Induced Fit hypothesis.

Enzyme inhibition, enzyme kinetics.

Diagnostic value of serum enzymes: Creatinine kinase, Alkaline phosphatase, Acid phosphatase, LDH, SGOT, SGPT, Amylase, Lipase, Carbonic anhydrase etc.

Practice:

Study of effect of temperature on enzyme activity

Study of effect of pH on enzyme activity

 

Unit- 2

Carbohydrates: Biomedical importance & properties of Carbohydrates, Classification

Families of monosaccharides: aldoses and ketoses, trioses, tetroses, pentoses, and hexoses. Stereo isomerism of monosaccharides, epimers, Haworth projection formulae for glucose; chair and boat forms of glucose.

Metabolism: Glycogenesis & glycogenolysis, Glycolysis, citric acid cycle & its significance, Components of respiratory chain, energy relationships during cell respiration, types of respiration. HMP shunt & Gluconeogenesis, regulation of blood glucose level.

Practice:

Estimation of Glucose in urine

Estimation of Glucose in blood

 

Unit- 3

Amino acids: Classification, essential & non-essential amino acids. Chemistry of Proteins & their related metabolism, Classification, biomedical importance.

Metabolism: Ammonia formation & transport, Transamination, Decarboxylation, Urea cycle, metabolic disorders in urea cycle, catabolism of amino acids.

Practice:

Estimation of Protein in urine

Estimation of Protein in blood

 

Unit- 4

Chemistry of Lipids & their related metabolism: Classification, biomedical importance, essential fatty acids. Brief out line of metabolism: Beta oxidation of fatty acids, fatty liver, Ketogenesis, Cholesterol & it's clinical significance, Lipoproteins in the blood composition & their functions in brief, Atherosclerosis.

Diabetes mellitus: its types, features, gestation diabetes mellitus, glucose tolerance test, glycosuria, Hypoglycaemia & its causes.

Practice:

Estimation of Bile pigment in urine

Estimation of Bile salts in urine

 

Suggested Readings:

  1. Victor W. Rodwell, David A. Bender, Kathleen M. Botham, Peter J. Kennelly, P. Anthony Weil (2018) Harper’s Illustrated Biochemistry. Mc Graw Hill.

(e-Book link: https://www.pdfdrive.com/harpers-illustrated-biochemistry-d176838999.html )

  1. Nelson DL and Cox MM. (2008). Lehninger Principles of Biochemistry, 5th Ed., W.H. Freeman and Company.

(e-Book link: https://www.pdfdrive.com/lehninger-principles-of-biochemistry-5th-edition-d164892141.html )

  1. Donald Voet, Judith G. Voet (2011) Biochemistry 4th Wiley Publishers.

(e-Book link: https://www.pdfdrive.com/biochemistry-4th-edition-e165192126.html )

  1. Jeremy M. Berg, John L. Tymoczko, Lubert Stryer. Biochemistry 7th W.H. Freeman and Company, New York.

(e-Book link: https://www.pdfdrive.com/biochemistry-seventh-edition-e167675390.html )

 

Simulation links for labs:

  1. Lecturio

(e-source link: https://app.lecturio.com/#/course/s/8014 )

  1. Labs for Life

(e-source link: http://labsforlife.in/InstructionalVideo.aspx )

 

Practice components can be performed by simulation as well as virtual labs.

Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).

Online Source: Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia

Session Plan

Session 1

Topic: Structure of enzyme: Apoenzyme and cofactors, prosthetic group-TPP, coenzyme NAD, metal cofactors

Video on Introduction on Enzymes

https://app.lecturio.com/#/lecture/s/8184/36358

https://www.youtube.com/watch?v=qgVFkRn8f10

https://www.youtube.com/watch?v=yk14dOOvwMk

Slideshare link to Introduction to Enzymes

https://www.slideshare.net/RichardBader/enzymes-14106495

https://www.slideshare.net/Ramzanken/enzymes-biochemistry

Session 2

Topic: Classification of enzymes

Video on Classification of enzymes

https://www.youtube.com/watch?v=0Nr9Pc00qO

Slideshare link to Classification of enzymes

https://www.slideshare.net/rakhiadarsh/classification-of-enzymes

https://www.slideshare.net/ArunViswanathan3/enzyme-199841983

Session 3

Topic: Mechanism of action of enzymes: active site, transition state complex and activation energy

Video on Mechanism of action of enzymes

https://app.lecturio.com/#/course/s/8242

https://www.youtube.com/watch?v=bTvG0UTJt9E

https://www.youtube.com/watch?v=ie_7Y7HJlps

https://www.youtube.com/watch?v=1BoZfsARSjk

Slideshare link on Mechanism of action of enzymes

https://www.slideshare.net/karthi131087/final-44690151

Session 4

Topic: Lock and key hypothesis, and Induced Fit hypothesis

Video on Lock and key hypothesis, and Induced Fit hypothesis

https://www.youtube.com/watch?v=MHN9FLuJtXY

https://www.youtube.com/watch?v=hXeMb-CBTW0

https://www.youtube.com/watch?v=EiMBsgNZh-M

https://www.youtube.com/watch?v=V8TOWTp71tE

https://www.youtube.com/watch?v=pVoytz_3H_s

Slideshare link to Lock and key hypothesis, and Induced Fit hypothesis

https://www.slideshare.net/DeepakKumarGupta2/enzyme-50519282

 

Session 5

Session 6

Session 7

Topic: Diagnostic value of serum enzymes - Creatinine kinase, Alkaline phosphatase, Acid phosphatase, LDH, SGOT, SGPT, Amylase, Lipase, Carbonic anhydrase

Video on Diagnostic value of serum enzymes

https://www.youtube.com/watch?v=c4CvXTYimck

https://www.youtube.com/watch?v=6UBcX8FE4YE

https://www.youtube.com/watch?v=aZni6VaXa8w

Session 8

Practice session

Topic: Study of effect of temperature on enzyme activity

Video on Study of effect of temperature on enzyme activity

https://www.youtube.com/watch?v=4vCZHLTSK1Q

https://www.youtube.com/watch?v=lXF6yjdyIgY

https://www.youtube.com/watch?v=1Fa2sSIt4_I

Procedure to perform Study of effect of temperature on enzyme activity

http://vlab.amrita.edu/?sub=3&brch=64&sim=1342&cnt=2

http://btc-bti.com/science101/pdf_files/Teacher's%20Guide%20Lab%203.pdf

Session 9

Session 10

Topic: Biomedical importance, properties, classification of Carbohydrates

Video on Introduction to Carbohydrates

https://www.youtube.com/watch?v=Do2Txci95n8

https://www.youtube.com/watch?v=3KJMYWvH034

Slideshare link to Introduction to Carbohydrates

https://www.slideshare.net/syed_ismail/carbohydrates-28872311

Session 11

Topic: Families of monosaccharides: aldoses and ketoses, trioses, tetroses, pentoses, and hexoses

Video on Monosaccharides

https://www.youtube.com/watch?v=p0soyMv8-Xo

Slideshare link to Monosaccharides

https://www.slideshare.net/SaifulIslam750/carbohydrates-and-its-classification

Session 12

Topic: Stereo isomerism of monosaccharides, epimers

Video on Stereo isomerism of monosaccharides, epimers

https://www.youtube.com/watch?v=o2CBC-ylqUs

https://www.youtube.com/watch?v=3KJMYWvH034

Session 13

Topic: Haworth projection formulae for glucose; chair and boat forms of glucose

Video on Haworth projection formulae for glucose; chair and boat forms of glucose

https://www.youtube.com/watch?v=IOv_I4HGsAE

Session 14

Session 15

Session 16

Session 17

Session 18

Session 19

Session 20

Topic: Types of respiration

Video on Types of respiration

https://www.youtube.com/watch?v=39HTpUG1MwQ

Slideshare link to Types of respiration

https://www.slideshare.net/FIRDOUS88/respiration-12818683

Session 21

Session 22

Session 23

Session 24

Practice session

Topic:Estimation of Glucose in urine

Video on Estimation of Glucose in urine

https://www.youtube.com/watch?v=-atHARq0JbQ

https://www.youtube.com/watch?v=WmBHnmQvXKE

Procedure to perform Estimation of Glucose in urine

http://www.gmcsurat.edu.in/lib/exe/fetch.php?media=biochemistry:method_of_glucose_analysis_2018.pdf

Session 25

Practice session

Topic: Estimation of Glucose in blood

Video on Estimation of Glucose in blood

https://www.youtube.com/watch?v=DyGIvs9zrVA

Procedure to perform Estimation of Glucose in blood

https://www.healthline.com/health/blood-sugar-tests#sources

Session 26

Session 27

Topic: Classification, essential & non-essential amino acids

Video on Classification, essential & non-essential amino acids

https://www.youtube.com/watch?v=PmbcA1Sav7s

https://www.youtube.com/watch?v=Mfo8jItCnBI

Slideshare link to Classification, essential & non-essential amino acids

https://www.slideshare.net/gangadharchatterjee/chemistry-of-amino-acids-and-protein

https://www.slideshare.net/ArjunKumar314/classification-of-amino-acids-81980287

https://www.slideshare.net/madhula/amino-acids-55336946

Session 28

Topic: Chemistry of Proteins & their related metabolism

Video on Chemistry of Proteins & their related metabolism

https://www.youtube.com/watch?v=HSCUAjZQhXI

https://www.youtube.com/watch?v=d8PxCiW3j24

https://www.youtube.com/watch?v=wvTv8TqWC48

https://www.youtube.com/watch?v=HL7-fYBtJ1Y

Slideshare link to Chemistry of Proteins

Session 29

Topic: Classification of Proteins

Video on Classification of Proteins

https://www.youtube.com/watch?v=cXBubSuR-2w

Session 30

Topic: Biomedical importance of Proteins

Video on Biomedical importance

https://www.youtube.com/watch?v=KSKPgaSGSYA

Session 31

Topic: Ammonia formation & transport

Video on Ammonia formation & transport

https://www.youtube.com/watch?v=8Ml4swq5LyA

Slideshare link to Ammonia formation & transport

https://www.slideshare.net/rohinisane/metabolism-of-ammonia

Session 32

Topic: Transamination, Decarboxylation

Video on Transamination, Decarboxylation

https://www.youtube.com/watch?v=Sao0NaY4IL0

Slideshare link to Transamination

https://www.slideshare.net/rohinisane/transamination-deamination-69296197

Session 33

Topic: Urea cycle, metabolic disorders in urea cycle

Video on Urea cycle, metabolic disorders in urea cycle

https://www.youtube.com/watch?v=Zrh4rvq_z4g

https://www.youtube.com/watch?v=a9sX-0YvIuk&t=93s

https://www.youtube.com/watch?v=Gt-hNq9ZHSk

Slideshare link to Urea cycle, metabolic disorders in urea cycle

https://www.slideshare.net/maulikpatel180/urea-cycle-120188130

Session 34

Session 35

Practice session

Topic: Estimation of Protein in urine

Video on Estimation of Protein in urine

https://www.youtube.com/watch?v=cRb1qtitT1M

Procedure to perform Estimation of Protein in urine

https://www.jbc.org/content/176/3/1421.full.pdf

https://www.portea.com/labs/diagnostic-tests/urine-protein-24-hour-urinary-protein-4/

Session 36

Practice session

Topic: Estimation of Protein in blood

Video on Estimation of Protein in blood

https://www.youtube.com/watch?v=3id3MJlpFKk

Procedure to perform Protein in blood

http://www.lfp.cuni.cz/biochemie/en/pages/vyuka/materialy/PRO_I.pdf

Session 37

Session 38

Topic: Classification of Lipids

Video on Classification of Lipids

https://www.youtube.com/watch?v=wxjwAD6WXP8

Session 39

Topic: Biomedical importance of Lipids

Video on Biomedical importance of Lipids

https://www.youtube.com/watch?v=PkKH8lTxvzA

Slideshare link on Biomedical importance of Lipids

https://www.slideshare.net/namarta28/lipid-chemistry

Session 40

Topic: Essential fatty acids

Video on Essential fatty acids

https://www.youtube.com/watch?v=Sh3eoIzdrdI

Slideshare link to Essential fatty acids

https://www.slideshare.net/DrLouay/essential-fatty-acids-58194571

Session 41

Topic: Beta oxidation of fatty acids

Video on Beta oxidation of fatty acids

https://www.youtube.com/watch?v=__jS-pjzb5k

https://www.youtube.com/watch?v=ZufZvbhPpws

https://www.youtube.com/watch?v=slCmrtFHFQQ

Slideshare link to Beta oxidation of fatty acids

https://www.slideshare.net/YESANNA/betaoxidation-of-fatty-acids

Session 42

Session 43

Session 44

Topic: Cholesterol & it's clinical significance

Video on Cholesterol & it's clinical significance

https://www.youtube.com/watch?v=0U7YHRW5dyc

https://www.youtube.com/watch?v=PkKH8lTxvzA&t=4s

https://www.youtube.com/watch?v=0U7YHRW5dyc

Slideshare link to Cholesterol & it's clinical significance

https://www.slideshare.net/melbiashine/cholestrol-amp-its-significance

Session 45

Topic: Lipoproteins in the blood composition & their functions

Video on Lipoproteins in the blood composition & their functions

https://www.youtube.com/watch?v=PkKH8lTxvzA

Slideshare link to Lipoproteins in the blood composition & their functions

https://www.slideshare.net/NikhilManoharan1/lipoprotein-95913913

Session 46

Session 47

Session 48

Session 49

Session 50

Session 51

Practice session

Topic:Estimation of Bile pigment in urine

Video on Estimation of Bile pigment in urine

https://www.youtube.com/watch?v=vHpTwihAx5U

Procedure to perform Estimation of Bile pigment in urine

https://www.myupchar.com/en/test/bile-pigment

Session 52

Practice session

Topic:Estimation of Bile pigment in urine

Video on Estimation of Bile pigment in urine

https://www.youtube.com/watch?v=xcJ31KS8U6M

https://www.youtube.com/watch?v=ac8gXAvHSPo

https://amrita.olabs.edu.in/?sub=79&brch=17&sim=211&cnt=1

Case Studies

Case Studies

Our Main Teachers

Dr. Soumya Jal

Associate Professor
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Dr. Soumya Jal is working at Centurion University of Technology and Management since 2018, prior to which she was associated with Rama Devi Women’s University as a visiting faculty.  She has completed her Ph. D. in Medical Biotechnology from VIT University, Vellore (2016). Her research area includes fields such as, Applied and Clinical Microbiology, Molecular […]