Biochemistry

Teacher

Dr. Soumya Jal

Category

Course Attendees

Still no participant

Course Reviews

Still no reviews

Course Name : Biochemistry

Code(Credit) : CUTM1732 (3-1-0)

Course Objectives

  • To understand the concept of metabolism of carbohydrates
  • To understand the significance of amino acids, proteins
  • Use of enzymes in enhancing metabolic reactions
  • Role of lipids

Learning Outcomes

  • After completion of the course the student will be developed a very good understanding of various biomolecules which are required for development and functioning of cells.
  • Would have understood the significance of carbohydrates in energy generation and as storage food molecules for cells.
  • They would have understood the significance of proteins and enzymes in accelerating various metabolic activities.
  • The conceptual understanding of the subject provides opportunities for skill enhancement and scopes for higher education.

Course Syllabus

Unit-1

Structure of enzyme: Apoenzyme and cofactors, prosthetic group-TPP, coenzyme NAD, metal cofactors, Classification of enzymes.

Mechanism of action of enzymes: active site, transition state complex and activation energy. Lock and key hypothesis, and Induced Fit hypothesis.

Enzyme inhibition, enzyme kinetics.

Diagnostic value of serum enzymes: Creatinine kinase, Alkaline phosphatase, Acid phosphatase, LDH, SGOT, SGPT, Amylase, Lipase, Carbonic anhydrase etc.

Practice:

Study of effect of temperature on enzyme activity

Study of effect of pH on enzyme activity

 

Unit- 2

Carbohydrates: Biomedical importance & properties of Carbohydrates, Classification

Families of monosaccharides: aldoses and ketoses, trioses, tetroses, pentoses, and hexoses. Stereo isomerism of monosaccharides, epimers, Haworth projection formulae for glucose; chair and boat forms of glucose.

Metabolism: Glycogenesis & glycogenolysis, Glycolysis, citric acid cycle & its significance, Components of respiratory chain, energy relationships during cell respiration, types of respiration. HMP shunt & Gluconeogenesis, regulation of blood glucose level.

Practice:

Estimation of Glucose in urine

Estimation of Glucose in blood

 

Unit- 3

Amino acids: Classification, essential & non-essential amino acids. Chemistry of Proteins & their related metabolism, Classification, biomedical importance.

Metabolism: Ammonia formation & transport, Transamination, Decarboxylation, Urea cycle, metabolic disorders in urea cycle, catabolism of amino acids.

Practice:

Estimation of Protein in urine

Estimation of Protein in blood

 

Unit- 4

Chemistry of Lipids & their related metabolism: Classification, biomedical importance, essential fatty acids. Brief out line of metabolism: Beta oxidation of fatty acids, fatty liver, Ketogenesis, Cholesterol & it's clinical significance, Lipoproteins in the blood composition & their functions in brief, Atherosclerosis.

Diabetes mellitus: its types, features, gestation diabetes mellitus, glucose tolerance test, glycosuria, Hypoglycaemia & its causes.

Practice:

Estimation of Bile pigment in urine

Estimation of Bile salts in urine

 

Suggested Readings:

  1. Victor W. Rodwell, David A. Bender, Kathleen M. Botham, Peter J. Kennelly, P. Anthony Weil (2018) Harper’s Illustrated Biochemistry. Mc Graw Hill.

(e-Book link: https://www.pdfdrive.com/harpers-illustrated-biochemistry-d176838999.html )

  1. Nelson DL and Cox MM. (2008). Lehninger Principles of Biochemistry, 5th Ed., W.H. Freeman and Company.

(e-Book link: https://www.pdfdrive.com/lehninger-principles-of-biochemistry-5th-edition-d164892141.html )

  1. Donald Voet, Judith G. Voet (2011) Biochemistry 4th Wiley Publishers.

(e-Book link: https://www.pdfdrive.com/biochemistry-4th-edition-e165192126.html )

  1. Jeremy M. Berg, John L. Tymoczko, Lubert Stryer. Biochemistry 7th W.H. Freeman and Company, New York.

(e-Book link: https://www.pdfdrive.com/biochemistry-seventh-edition-e167675390.html )

 

Simulation links for labs:

  1. Lecturio

(e-source link: https://app.lecturio.com/#/course/s/8014 )

  1. Labs for Life

(e-source link: http://labsforlife.in/InstructionalVideo.aspx )

 

Practice components can be performed by simulation as well as virtual labs.

Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).

Online Source: Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia

Session Plan

Session 1

Topic: Structure of enzyme: Apoenzyme and cofactors, prosthetic group-TPP, coenzyme NAD, metal cofactors

Video on Introduction on Enzymes

https://app.lecturio.com/#/lecture/s/8184/36358

https://www.youtube.com/watch?v=qgVFkRn8f10

https://www.youtube.com/watch?v=yk14dOOvwMk

Slideshare link to Introduction to Enzymes

https://www.slideshare.net/RichardBader/enzymes-14106495

https://www.slideshare.net/Ramzanken/enzymes-biochemistry

Session 2

Topic: Classification of enzymes

Video on Classification of enzymes

https://www.youtube.com/watch?v=0Nr9Pc00qO

Slideshare link to Classification of enzymes

https://www.slideshare.net/rakhiadarsh/classification-of-enzymes

https://www.slideshare.net/ArunViswanathan3/enzyme-199841983

Session 3

Topic: Mechanism of action of enzymes: active site, transition state complex and activation energy

Video on Mechanism of action of enzymes

https://app.lecturio.com/#/course/s/8242

https://www.youtube.com/watch?v=bTvG0UTJt9E

https://www.youtube.com/watch?v=ie_7Y7HJlps

https://www.youtube.com/watch?v=1BoZfsARSjk

Slideshare link on Mechanism of action of enzymes

https://www.slideshare.net/karthi131087/final-44690151

Session 4

Topic: Lock and key hypothesis, and Induced Fit hypothesis

Video on Lock and key hypothesis, and Induced Fit hypothesis

https://www.youtube.com/watch?v=MHN9FLuJtXY

https://www.youtube.com/watch?v=hXeMb-CBTW0

https://www.youtube.com/watch?v=EiMBsgNZh-M

https://www.youtube.com/watch?v=V8TOWTp71tE

https://www.youtube.com/watch?v=pVoytz_3H_s

Slideshare link to Lock and key hypothesis, and Induced Fit hypothesis

https://www.slideshare.net/DeepakKumarGupta2/enzyme-50519282

 

Session 5

Session 6

Session 7

Topic: Diagnostic value of serum enzymes - Creatinine kinase, Alkaline phosphatase, Acid phosphatase, LDH, SGOT, SGPT, Amylase, Lipase, Carbonic anhydrase

Video on Diagnostic value of serum enzymes

https://www.youtube.com/watch?v=c4CvXTYimck

https://www.youtube.com/watch?v=6UBcX8FE4YE

https://www.youtube.com/watch?v=aZni6VaXa8w

Session 8

Practice session

Topic: Study of effect of temperature on enzyme activity

Video on Study of effect of temperature on enzyme activity

https://www.youtube.com/watch?v=4vCZHLTSK1Q

https://www.youtube.com/watch?v=lXF6yjdyIgY

https://www.youtube.com/watch?v=1Fa2sSIt4_I

Procedure to perform Study of effect of temperature on enzyme activity

http://vlab.amrita.edu/?sub=3&brch=64&sim=1342&cnt=2

http://btc-bti.com/science101/pdf_files/Teacher's%20Guide%20Lab%203.pdf

Session 9

Session 10

Topic: Biomedical importance, properties, classification of Carbohydrates

Video on Introduction to Carbohydrates

https://www.youtube.com/watch?v=Do2Txci95n8

https://www.youtube.com/watch?v=3KJMYWvH034

Slideshare link to Introduction to Carbohydrates

https://www.slideshare.net/syed_ismail/carbohydrates-28872311

Session 11

Topic: Families of monosaccharides: aldoses and ketoses, trioses, tetroses, pentoses, and hexoses

Video on Monosaccharides

https://www.youtube.com/watch?v=p0soyMv8-Xo

Slideshare link to Monosaccharides

https://www.slideshare.net/SaifulIslam750/carbohydrates-and-its-classification

Session 12

Topic: Stereo isomerism of monosaccharides, epimers

Video on Stereo isomerism of monosaccharides, epimers

https://www.youtube.com/watch?v=o2CBC-ylqUs

https://www.youtube.com/watch?v=3KJMYWvH034

Session 13

Topic: Haworth projection formulae for glucose; chair and boat forms of glucose

Video on Haworth projection formulae for glucose; chair and boat forms of glucose

https://www.youtube.com/watch?v=IOv_I4HGsAE

Session 14

Session 15

Session 16

Session 17

Session 18

Session 19

Session 20

Topic: Types of respiration

Video on Types of respiration

https://www.youtube.com/watch?v=39HTpUG1MwQ

Slideshare link to Types of respiration

https://www.slideshare.net/FIRDOUS88/respiration-12818683

Session 21

Session 22

Session 23

Session 24

Practice session

Topic:Estimation of Glucose in urine

Video on Estimation of Glucose in urine

https://www.youtube.com/watch?v=-atHARq0JbQ

https://www.youtube.com/watch?v=WmBHnmQvXKE

Procedure to perform Estimation of Glucose in urine

http://www.gmcsurat.edu.in/lib/exe/fetch.php?media=biochemistry:method_of_glucose_analysis_2018.pdf

Session 25

Practice session

Topic: Estimation of Glucose in blood

Video on Estimation of Glucose in blood

https://www.youtube.com/watch?v=DyGIvs9zrVA

Procedure to perform Estimation of Glucose in blood

https://www.healthline.com/health/blood-sugar-tests#sources

Session 26

Session 27

Topic: Classification, essential & non-essential amino acids

Video on Classification, essential & non-essential amino acids

https://www.youtube.com/watch?v=PmbcA1Sav7s

https://www.youtube.com/watch?v=Mfo8jItCnBI

Slideshare link to Classification, essential & non-essential amino acids

https://www.slideshare.net/gangadharchatterjee/chemistry-of-amino-acids-and-protein

https://www.slideshare.net/ArjunKumar314/classification-of-amino-acids-81980287

https://www.slideshare.net/madhula/amino-acids-55336946

Session 28

Topic: Chemistry of Proteins & their related metabolism

Video on Chemistry of Proteins & their related metabolism

https://www.youtube.com/watch?v=HSCUAjZQhXI

https://www.youtube.com/watch?v=d8PxCiW3j24

https://www.youtube.com/watch?v=wvTv8TqWC48

https://www.youtube.com/watch?v=HL7-fYBtJ1Y

Slideshare link to Chemistry of Proteins

Session 29

Topic: Classification of Proteins

Video on Classification of Proteins

https://www.youtube.com/watch?v=cXBubSuR-2w

Session 30

Topic: Biomedical importance of Proteins

Video on Biomedical importance

https://www.youtube.com/watch?v=KSKPgaSGSYA

Session 31

Topic: Ammonia formation & transport

Video on Ammonia formation & transport

https://www.youtube.com/watch?v=8Ml4swq5LyA

Slideshare link to Ammonia formation & transport

https://www.slideshare.net/rohinisane/metabolism-of-ammonia

Session 32

Topic: Transamination, Decarboxylation

Video on Transamination, Decarboxylation

https://www.youtube.com/watch?v=Sao0NaY4IL0

Slideshare link to Transamination

https://www.slideshare.net/rohinisane/transamination-deamination-69296197

Session 33

Topic: Urea cycle, metabolic disorders in urea cycle

Video on Urea cycle, metabolic disorders in urea cycle

https://www.youtube.com/watch?v=Zrh4rvq_z4g

https://www.youtube.com/watch?v=a9sX-0YvIuk&t=93s

https://www.youtube.com/watch?v=Gt-hNq9ZHSk

Slideshare link to Urea cycle, metabolic disorders in urea cycle

https://www.slideshare.net/maulikpatel180/urea-cycle-120188130

Session 34

Session 35

Practice session

Topic: Estimation of Protein in urine

Video on Estimation of Protein in urine

https://www.youtube.com/watch?v=cRb1qtitT1M

Procedure to perform Estimation of Protein in urine

https://www.jbc.org/content/176/3/1421.full.pdf

https://www.portea.com/labs/diagnostic-tests/urine-protein-24-hour-urinary-protein-4/

Session 36

Practice session

Topic: Estimation of Protein in blood

Video on Estimation of Protein in blood

https://www.youtube.com/watch?v=3id3MJlpFKk

Procedure to perform Protein in blood

http://www.lfp.cuni.cz/biochemie/en/pages/vyuka/materialy/PRO_I.pdf

Session 37

Session 38

Topic: Classification of Lipids

Video on Classification of Lipids

https://www.youtube.com/watch?v=wxjwAD6WXP8

Session 39

Topic: Biomedical importance of Lipids

Video on Biomedical importance of Lipids

https://www.youtube.com/watch?v=PkKH8lTxvzA

Slideshare link on Biomedical importance of Lipids

https://www.slideshare.net/namarta28/lipid-chemistry

Session 40

Topic: Essential fatty acids

Video on Essential fatty acids

https://www.youtube.com/watch?v=Sh3eoIzdrdI

Slideshare link to Essential fatty acids

https://www.slideshare.net/DrLouay/essential-fatty-acids-58194571

Session 41

Topic: Beta oxidation of fatty acids

Video on Beta oxidation of fatty acids

https://www.youtube.com/watch?v=__jS-pjzb5k

https://www.youtube.com/watch?v=ZufZvbhPpws

https://www.youtube.com/watch?v=slCmrtFHFQQ

Slideshare link to Beta oxidation of fatty acids

https://www.slideshare.net/YESANNA/betaoxidation-of-fatty-acids

Session 42

Session 43

Session 44

Topic: Cholesterol & it's clinical significance

Video on Cholesterol & it's clinical significance

https://www.youtube.com/watch?v=0U7YHRW5dyc

https://www.youtube.com/watch?v=PkKH8lTxvzA&t=4s

https://www.youtube.com/watch?v=0U7YHRW5dyc

Slideshare link to Cholesterol & it's clinical significance

https://www.slideshare.net/melbiashine/cholestrol-amp-its-significance

Session 45

Topic: Lipoproteins in the blood composition & their functions

Video on Lipoproteins in the blood composition & their functions

https://www.youtube.com/watch?v=PkKH8lTxvzA

Slideshare link to Lipoproteins in the blood composition & their functions

https://www.slideshare.net/NikhilManoharan1/lipoprotein-95913913

Session 46

Session 47

Session 48

Session 49

Session 50

Session 51

Practice session

Topic:Estimation of Bile pigment in urine

Video on Estimation of Bile pigment in urine

https://www.youtube.com/watch?v=vHpTwihAx5U

Procedure to perform Estimation of Bile pigment in urine

https://www.myupchar.com/en/test/bile-pigment

Session 52

Practice session

Topic:Estimation of Bile pigment in urine

Video on Estimation of Bile pigment in urine

https://www.youtube.com/watch?v=xcJ31KS8U6M

https://www.youtube.com/watch?v=ac8gXAvHSPo

https://amrita.olabs.edu.in/?sub=79&brch=17&sim=211&cnt=1

Case Studies

Case Studies

Recent Comments

    Our Main Teachers

    Dr. Soumya Jal

    Associate Professor
    VIEW PROFILE

    Dr. Soumya Jal is working at Centurion University of Technology and Management since 2018, prior to which she was associated with Rama Devi Women’s University as a visiting faculty.  She has completed her Ph. D. in Medical Biotechnology from VIT University, Vellore (2016). Her research area includes fields such as, Applied and Clinical Microbiology, Molecular […]