Biotechnology in Pharmaceutical Sciences
Teacher
TAMOSA MUKHERJEE
Category
Core Courses
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Course Objective
The course will cover in depth knowledge of Forensic Biotechnology in Pharmaceutical Science, it's disciplines and their importance when working in FSL.
Course Outcomes
- The significance of forensic biotechnology in pharmaceutical sciences
- The fundamental principles, functions and application of industrial products in forensic science
- The uses of industrial microbiology in forensic science laboratory
- The working of the regulatory affairs in forensic establishments in India
Course Syllabus
Unit-I
Introduction to Recombinant DNA Technology
DNA Modifying enzymes. Cloning strategies: Genomic libraries, cDNA libraries, single gene cloning. RAPD, RFLP and AFLP. Vectors: Types of vectors and choice of vectors : Plasmids, Cosmids, lambda phage, shuttle vectors, bacs and yacs. Transformation and Transfection. Expression system in Eukaryotic cells, Yeast, Bacteria, Insect cell lines, Gene screening. Biosafety guidelines and containment startegies
Unit-II (Bioprocess and Bio-chemical engineering)
Bioreactor design: Body construction, aeration and agitation, operation and applications. Microbial growth: measurement, batch and continuous culture and its kinetics. Downstream processing: recovery and purification of products. Strain improvement. Fermentation economics
Unit-III (Animal and Plant Biotechnology)
Cell lines: definition, development, maintenance and management , established cell lines and their characteristic features. Transgenic animals : creating transgenic animals, examples of transgenic animals - Dolly, insects, primates, mice. Somaclonal and gameto clonal variation: applications and limitations. Transgenic Plants: Herbicide resistant, insect Resistant, drought/stress resistant, delayed ripening, Ti Plasmid and T-DNA transfer. Gene therapy: potential approach to gene therapy. Southern blotting, Northern blotting, Western blotting, Iso-electric focusing. Overview of Stem cells and its applications
Unit-IV (Biotherapeutics and Industrial Microbiology)
Introduction to industry important recombinant proteins, recombinant protein stability. Improvised recombinant protein secretion. Introduction to industrially important primary and secondary metabolites from plants and microbes. Microbial production: Antibiotics, acetic acid, lactic acid, wine, beer, SCP
Unit-V (Regulatory affairs and IPR)
Basic principles of quality control (QC) and quality assurance (QA). Guidelines for QA and QC: raw materials, products and validation. Introduction to pharmacopoeia. Intellectual Property Rights. Importance of protecting scientific discoveries. IPR policy of Government of India. Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.
Reference Books
- Pharmaceutical Biotechnology : Concepts & Applications – Gary Walsh (Wiley)
- Modern Industrial Microbiology and Biotechnology – Nduka Okafor (Sciences Publisher)
- Biotechnology and Biopharmaceuticals – Rodney J.Y.H.O and Milo Gibaldi (Wiley)
- Biotechnology in Healthcare – Gavin Brooks (PLP)
- Gene Therapy: Protocols - Joseph M. Le Doux (Humana Press)
- Biotehnology – Applying the genetic revolution – David P. Clark and Nanette J. Pazdarnik, Academic Press, ISBN: 978-0121755522
- Molecular Biotechnology: Principles and Applications of Recombinant DNA, 4th Edition –
Bernard G. Glick, Jack J. Pasternak and Cheryl L. Patten, ASM Press, ISBN: 978-1555814984
Session Plan
Session-1
DNA Modifying enzymes mod2 DNADNA Modifying Enzymes
Session-2
Cloning strategies: Genomic Libraries04_M109nys091016_19_23
Session-3
cDNA libraries, single gene cloning Send Genomic and cDNA libraries04_M109nys091016_19_23
Session-4
RAPD, RFLP and AFLP aflp2aflpRandom_amplified_polymorphic_DNA_RAPD_markers_and_18591_Restriction Fragment Length PolymorphismInTech-Restriction_fragment_length_polymorphism_analysis_of_pcr_amplified_fragments_pcr_rflp_and_gel_electrophoresis_valuable_tool_for_genotyping_and_genetic_fingerprintingrflp
Session-5
Vectors: Types of vectorsMSc-Biotech-02Sem-ProfBhuminathTripathi
Session-6
Choice of vectors: Plasmids, Cosmid, Lambda Phage vectors119_Cloning vectors
Session-7
Shuttle vectors, BACS, YACSSend Cloning Vectors-1
Session-8
Transformation and Transfection transfection-slideshowpeerj-111653 Transformation
Session-9
Expression system in Eukaryotic cells, Yeast, Bacteriaexpression_systems
Session-10
Insect cell lines, Gene screeningexpression_systemsIntroduction_to_Genetic_Screening
Session-11
Biosafety guidelines and containment strategies
Session-12
Bioreactor body construction. Aeration, agitation. Operation and Principals30042020085526-BIOREACTOR__BASICS-converted_(1)
Session-13
Microbial growth: Measurement 04_Chapter_33__Microbial_Nutrition_and_Growth_07Chap06Growth1834_indirectmethodsofmeasurementofgrowth
Session-14
Batch and continuous culture and its kinetics
https://www.youtube.com/watch?v=mUDXupn2Dhk
Session-15
Downstream processing: Recovery and purification of productsSend Downstream Processing-1
Session-16
Strain ImprovementSend Strain Improvement
Session-17
Fermentation Economics
Session-18
Cell lines: Definition, development, maintenance and management, established cell lines and their characteristic features.
- Cell lines: Definition, development, maintenance and management, established cell lines andtheir characteristic features.Send Animal Cell LinesSend Animal Cell Lines
Session-19
Transgenic animals- Creating transgenic animals, Example of transgenic animals-DollySend Transgenic Animals
Session-20
Insects, Primates, miceSend Transgenic Animals
Session-21
Somaclonal and gameto clonal variationSend UDAY.SOMACLONAL.
Session-22
applications and limitations
Session-23
Transgenic Plants: Herbicide resistant, insect ResistantSend Transgenic Plants
Session-24
drought/stress resistant, delayed ripeningSend ayaskanta ppt
Session-25
Ti Plasmid and T-DNA transfer.
Session-26
Gene therapy: potential approach to gene therapyGene Therapy-1
Session-28
Western blotting, Iso-electric focusingSend blotting
Session-29
Overview of Stem cells and its applicationsstem cells
Session-30
Introduction to industry important recombinant proteins, recombinant protein stabilitymicrobial recombinant protein
Session-31
Improvised recombinant protein secretion
Session-32
Introduction to industrially important primary and secondary metabolites from plants and microbes
Session-33
Microbial production: Antibiotics, acetic acid, lactic acid Production of Lactic AcidProduction of Acetic Acid
Session-35
Basic principles of quality control (QC) and quality assurance (QA)_QA-QC
Session-36
Guidelines for QA and QC: raw materials, products and validation
Session-37
Introduction to pharmacopoeia000214416
Session-38
Intellectual Property RightsINTELLECTUAL-PROPERTY-RIGHTS_20.04.2020
Session-39
Importance of protecting scientific discoveries
IPR policy of Government of IndiaIntellectual Property Right (IPR) and Protection(IPP
Session-40
Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.
Syllabus for Practical :
Session-40
Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.
Session-40
Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.
Session-40
Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.
Session-40
Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.
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