Cell and Molecular Biology

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Dr. Soumya Jal

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Course Name : Cell and Molecular Biology

Code(Credit) : CUTM1714 (2-0-1)

Course Objectives

  • Understanding the central dogma of life
  • To understand the concept of gene regulation and its impact
  • The use of several molecular diagnostic techniques for disease interpretation

Learning Outcomes

  • After completion of the course the student will be gain knowledge of the significance of genes and proteins.
  • They will understand the mechanism of gene expression and protein synthesis,
  • The significance of gene expression regulation will become clear.
  • The students will understand the use of several molecular techniques in disease diagnosis.

Course Syllabus

Module-1

DNA structure. Salient features of double helix, Types of DNA. DNA topology - linking number, topoisomerases. Bidirectional and unidirectional replication, semi- conservative, semi- discontinuous replication.

Mechanism of DNA replication. Enzymes and proteins involved in DNA replication.

 

Module-2

RNA Structure. Transcription: Promoter, Polymerase and the transcription unit. Transcription in Eukaryotes: RNA polymerases, general Transcription factors. Split genes, concept of introns and exons, RNA splicing, spliceosome machinery, concept of alternative splicing, Polyadenylation and capping, Processing of rRNA, RNA interference: si RNA, miRNA and its significance.

Translational machinery: Charging of tRNA, aminoacyl tRNA synthetases. Mechanisms of initiation, elongation and termination of polypeptides in both prokaryotes and eukaryotes.

Malignant transformation of cells and role of oncogenes, Tumor virus, Proto- oncogenes, Tumor suppressor genes; Apoptosis, cell regeneration.

 

Module- 3

Molecular diagnostics: Recombinant DNA Technology and its applications, Polymerase chain reaction and its application in diagnosis of pathogens, Site directed mutagenesis, DNA finger printing, DNase Foot Printing, antisense RNA technology, inherited genetic disorders in man and gene therapy.

 

Suggested Readings:

  1. Watson JD, Baker TA, Bell SP, Gann A, Levine M and Losick R (2008) Molecular Biology of the Gene, 7th edition, Cold Spring Harbour Lab. Press, Pearson Publication.

(e-Book link: https://www.pdfdrive.com/molecular-biology-of-the-gene-e158278674.html )

  1. Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter (2015) Molecular Biology of the cell, 6th edition, Taylor and Francis Group.

(e-Book link: https://www.pdfdrive.com/molecular-biology-of-the-cell-d184612905.html )

  1. Principles and Practice of Medicine- by Davidson, S. S., J. MacLeod and C.R.W. Edwards,1991 Publisher Churchill Livingstone.

(e-Book link: https://www.pdfdrive.com/davidsons-principles-and-practice-of-medicine-d186204495.html )

  1. Sambrook J and Russell DW. (2001). Molecular Cloning: A Laboratory Manual. 4th Edition, Cold Spring Harbour Laboratory press.

(e-Book link: https://www.pdfdrive.com/search?q=Sambrook+J+and+Russell+DW.+%282001%29.+Molecular+Cloning%3A+A+Laboratory+Manual.+4th+Edition&pagecount=&pubyear=&searchin=&more=true )

  1. Geoffrey M. Cooper, Robert E. Hausman (2007). The Cell, A molecular approach. 4th ASM Press, Washington, D.C.

(e-Book link: https://www.pdfdrive.com/the-cell-a-molecular-approach-e186369576.html )

  1. B. Primrose and R.M. Twyman (2006) Principles of Gene Manipulation and Genomics 7th Edition. Blackwell Publishing.

(e-Book link: https://www.pdfdrive.com/principles-of-gene-manipulation-and-genomics-e25845509.html )

 

Online Tutorial links:

  1. DNA Decoded

(coursera link: https://www.coursera.org/learn/dna-decoded )

  1. DNA Replication

(Lecturio link: https://app.lecturio.com/#/course/s/8020 )

  1. Transcription

(Lecturio link: https://app.lecturio.com/#/lecture/s/5990/35832 )

 

Simulation links for labs:

All the practice components can be performed by simulation as well as virtual labs.

Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).

Online Source: Labs for life, Lecturio, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia

Session 2

Topic: Types of DNA

Video on Types of DNA

https://www.youtube.com/watch?v=lJZhzGNipJc

Slideshare link to Types of DNA

https://www.slideshare.net/vruddhidesai/dna-strcture-and-function

Session 3

Topic: DNA topology - linking number, topoisomerases

Video on DNA topology - linking number, topoisomerases

https://app.lecturio.com/#/lecture/s/5990/35830

https://www.youtube.com/watch?v=o_-6JXLYS-k

Slideshare link to DNA topology

https://www.slideshare.net/lalvarezmex/dna-topology

Session 4

Topic: Bidirectional and unidirectional replication

Video on Bidirectional and unidirectional replication

https://app.lecturio.com/#/lecture/s/8020/35160

https://www.youtube.com/watch?v=QMX7IpME7X8

Session 5

Topic: Semi- conservative, semi- discontinuous replication

Video on Semi- conservative, semi- discontinuous replication

https://app.lecturio.com/#/lecture/s/5990/35830

https://app.lecturio.com/#/lecture/s/8020/35160

Slideshare link to  Semi- conservative replication

https://www.slideshare.net/manglam91/dna-replication-41555427?next_slideshow=1

https://www.slideshare.net/ashokktt/dna-replication-140786838

Session 6

Session 7

Topic: Enzymes and proteins involved in DNA replication

Video on Enzymes and proteins involved in DNA replication

https://app.lecturio.com/#/lecture/s/8020/35156

https://www.youtube.com/watch?v=QMX7IpME7X8

Session 8

Session 9

Topic: Promoter, Polymerase and the transcription unit

Video on Promoter, Polymerase and the transcription unit

https://www.youtube.com/watch?v=cXlv21NCGxQ

https://www.youtube.com/watch?v=XzVXhemtwmA

Session 10

Session 11

Topic: Split genes, concept of introns and exons

Video on Split genes, concept of introns and exons

https://www.youtube.com/watch?v=rPnfrXDHJKc

Session 12

Topic: RNA splicing, spliceosome machinery, concept of alternative splicing

Video on RNA splicing, spliceosome machinery, concept of alternative splicing

https://www.youtube.com/watch?v=rPnfrXDHJKc

https://www.youtube.com/watch?v=nUhChbEBC3c

https://www.youtube.com/watch?v=YgmoHtLGb5c

Slideshare link to RNA splicing

https://www.slideshare.net/msaltyy/rna-splicing-65637051

https://www.slideshare.net/askhambhati/rna-splicing

Session 13

Topic: Polyadenylation and capping

Video on Polyadenylation and capping

https://www.youtube.com/watch?v=YgmoHtLGb5c

Session 14

Topic: Processing of rRNA

Video on Processing of rRNA

https://www.youtube.com/watch?v=YjWuVrzvZYA

Session 15

Session 16

Session 17

Topic: Aminoacyl tRNA synthetases

Video: Aminoacyl tRNA synthetases

https://www.youtube.com/watch?v=5bLEDd-PSTQ

Session 18

Topic: Mechanisms of initiation, elongation and termination in Prokaryotes

Video on Mechanisms of initiation, elongation and termination in Prokaryotes

https://www.youtube.com/watch?v=6YqPLgNjR4Q

https://www.youtube.com/watch?v=G2yovIdpTVk

https://www.youtube.com/watch?v=MpO9ivZD-os

Slideshare link to Translation in Prokaryotes

https://www.slideshare.net/IllllIkrAmKhanIllll/translation-in-prokaryotes-and-eukaryotes-75358755

Session 19

Topic: Mechanisms of initiation, elongation and termination in Eukaryotes

Video on Mechanisms of initiation, elongation and termination in Eukaryotes

https://www.youtube.com/watch?v=G2yovIdpTVk

https://www.youtube.com/watch?v=MpO9ivZD-os

Slideshare link to Translation in Eukaryotes

https://www.slideshare.net/UmerFarooq160/translation-in-eukaryotes

Session 20

Topic: Malignant transformation of cells and role of oncogenes

Video on Malignant transformation of cells and role of oncogenes

https://www.youtube.com/watch?v=jjfYQMW_nek

https://www.youtube.com/watch?v=VTRAkVfEbbU

https://www.youtube.com/watch?v=XWfqBKeC0g8

https://www.youtube.com/watch?v=8uWWpdyf6_c

Slideshare link to Cancer development

https://www.slideshare.net/mprasadnaidu/oncogenes

Session 21

Session 22

Session 23

Session 24

Session 25

Topic: Cell regeneration

Video on Cell regeneration

https://www.youtube.com/watch?v=729eJkKZ84s&t=1049s

Slideshare link to Cell regeneration

https://www.slideshare.net/ImranaTanvir/cell-and-tissue-regeneration-71908744

Session 26

Session 27

Topic: Polymerase chain reaction and its application in diagnosis of pathogens

Video on Polymerase chain reaction and its application in diagnosis of pathogens

https://www.youtube.com/watch?v=0gIfj0jewWY

https://www.youtube.com/watch?v=MyLrs_h1OlE

https://www.youtube.com/watch?v=gubLAtn2o4s

Slideshare link to Polymerase chain reaction

https://www.slideshare.net/sara_abudahab/polymerase-chain-reaction-85874296

Session 28

Topic: Site directed mutagenesis

Video on Site directed mutagenesis

https://www.youtube.com/watch?v=7xFvaI7coyg

Slideshare link to Site directed mutagenesis

https://www.slideshare.net/ArunimaSur/site-directed-mutagenesis

Session 29

Session 30

Topic: DNase Foot Printing

Video on DNase Foot Printing

https://www.youtube.com/watch?v=gHpKL0edPLg

Session 31

Session 32

Case Studies

Case Studies

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    Dr. Soumya Jal

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    Dr. Soumya Jal is working at Centurion University of Technology and Management since 2018, prior to which she was associated with Rama Devi Women’s University as a visiting faculty.  She has completed her Ph. D. in Medical Biotechnology from VIT University, Vellore (2016). Her research area includes fields such as, Applied and Clinical Microbiology, Molecular […]