Cell Biology

Teacher

Sujit Kumar Mishra

Category

Core Courses

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Course Name : Cell Biology

Code (Credit) : CUTM1500 (3-2-1)

Course Objectives

  • To make the student understand all type of cells and cellular components, and how cell works in healthy and diseased states

Learning Outcomes

Students will understand

  • the structures and purposes of basic components of prokaryotic and eukaryotic cells.

  • how the cellular components are organized and perform specific functions.

  • the cellular components underlying mitotic and meiotic cell division.

  • the cellular communication and signal transduction takes place in cells

Course Syllabus

Module- 1 (11 Hrs)

 

Overview of Cells; Cell theory; Prokaryotic and Eukaryotic cells; Virus; Viroids; Mycoplasma; Prions

 

Assignment 1: Prions and associated diseases

 

Practice 1: Cell Organization and Sub Cellular Structure Studies

Practice 2: Gram’s staining technique for visualization of prokaryotic cells

Practice 3: Counting of cells using Hemocytometer

 

Module- 2 (10 Hrs)

 

Plasma Membrane: Various models of plasma membrane structure; Transport across membranes: Active and Passive transport, Facilitated Transport; Cell junctions: Tight junctions, Gap junctions, Desmosomes, Hemidesmosomes

 

Assignemnt 2: Understanding various models of plasma membrane structures

Assignment 3: Different type of transportation carried out in plasma membranes

 

Practice 4: Study of cell attachment

Practice 5: Study of cell proliferation

 

Module- 3 (6 Hrs)

 

Endomembrane System: Structure and Functions of Endoplasmic Reticulum, Golgi Apparatus, Lysosomes

 

Assignment 4: Understanding of Endomembrane system

 

Practice 6: Isolation of Endoplasmic Reticulum

 

 

Module- 4 (8 Hrs)

 

Mitochondria: Structure and function, Semi-autonomous nature, Endosymbiotic hypothesis, Chemi-osmotic hypothesis, Mitochondrial electron transport chain; Peroxisomes: structure and function.

 

Assignemnt 5: Semiautonomous nature of mitochondria

Assignment 6: Mitochondria: Endosymbiotic and chemiosmotic hypothesis

 

Practice 7: Isolation and microscopic study of mitochondria

 

Module- 5 (10 Hrs)

 

Cytoskeleton: Structure and Functions:  Microtubules, Microfilaments and Intermediate filaments; Nucleus: Structure of Nucleus: Nuclear envelope, Nuclear pore complex, Nucleolus, Chromatin: Euchromatin and Hetrochromatin and packaging (nucleosome)

 

Assignment 7: Structure and function of cytoskeleton

Assignment 8: Structure and function of Euchromatin and Heterochromatin

 

Practice 8: Study the presence of Barr body in human female blood cells/cheek cell

Practice 9: Study of Actin Assembly

 

Module- 6 (10 Hrs)

 

Cell Division and Cell cycle: Mitosis, Meiosis; Regulation of cell cycle

 

Practice 10: Study various stages of mitosis using permanent slides

Practice 11: Study various stages of meiosis using permanent slides

 

Module- 7 (10 Hrs)

 

Cell Signaling: Overview of cell signaling, signaling molecules and receptors, GPCR, Second messengers, Role of second messenger (cAMP) in cell signaling, Activation of gene transcription by GPCR

 

Assignment 9: Role of different signaling molecules in cell signaling

Assignment 10: Different signal receptors

 

Practice 12: Maintenance of mammalian cell lines

 

Text Books:

 

  1. Karp, G. (2010). Cell and Molecular Biology: Concepts and Experiments. VI John Wiley and Sons. Inc.

  2. De Robertis, E.D.P. and De Robertis, E.M.F. (2006). Cell and Molecular Biology. VIII Edition. Lippincott Williams and Wilkins

 

Reference Books:

 

  1. Cooper, G.M. and Hausman, R.E. (2009). The Cell: A Molecular Approach. V ASM Press and Sunderland, Washington, D.C.; Sinauer Associates, MA.

  2. Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009). The World of the Cell. VII Edition. Pearson Benjamin Cummings Publishing, San

  3. Bruce Albert, Bray Dennis, Levis Julian, Raff Martin, Roberts Keith and Watson James (2008). Molecular Biology of the Cell, V Edition, Garland publishing Inc., New York and London

Session Plan

Session 1

Session 8 (Assignment 1/Flip class)

Session 9 (Practice 1: 2 Hrs)

Gram’s staining technique for visualization of prokaryotic cells

http://vlab.amrita.edu/?sub=3&brch=73&sim=208&cnt=1

Session 10 (Practice 2: 2 Hrs)

Session 11 (Practice 3: 2 Hrs)

Counting of cells using Hemocytometer

http://cbii-au.vlabs.ac.in/cell-biology-2/Hemocytometer/

Session 12 (Assignment 2/Flip class)

Plasma Membrane: Various models of plasma membrane structure

Video: https://www.youtube.com/watch?v=Mp3yoHcC63E

PPT: https://www.slideshare.net/rajpalchoudharyjat/plasma-membrane-71915632

Session 13 (Assignment 3/Flip class)

Transport across plasma membrane: Active and Passive transport, Facilitated transport

Video: https://www.youtube.com/watch?v=J5pWH1r3pgU

PPT: https://www.slideshare.net/nileshkate79/transport-across-cell-membrane-54062942

Session 14 (Practice 4: 2 Hrs)

Session 15 (Practice 5: 2 Hrs)

Session 16

Session 17

Session 20

Session 22 (Practice 6: 2 Hrs)

Session 24 (Assignment 5/Flip class)

Mitochondria: Semi-autonomous nature

PPT: https://www.slideshare.net/Dilippandya/mitochondria-46636401

Session 25 (Assignment 6/Flip class)

Session 28 (Practice 7: 2 Hrs)

Isolation and microscopic study of mitochondria

http://cbi-au.vlabs.ac.in/cell-biology-1/Isolation_of_Mitochondria/

Session 29 (Assignment 7/Flip class)

Session 32

Session 33

Nucleus: Structure of Nucleus: Nuclear envelope, Nuclear pore complex, Nucleolus

Video: https://www.youtube.com/watch?v=nD2Fd0KyICA

PPT: https://www.slideshare.net/Salman437/nucleus-structure

Session 34 (Assignment 8/Flip class)

Session 35

Chromatin organization and packaging (nucleosome)

Video: https://www.youtube.com/watch?v=F1opjc3PsC4

PPT: https://www.slideshare.net/sheetalnarkar01/nucleosomes

Session 36 (Practice 8: 2 Hrs)

Study the presence of Barr body in human female blood cells/cheek cells.

https://www.youtube.com/watch?v=NqDdh6sLnus

Session 37 (Practice 9: 2 Hrs)

Session 43 (Practice 10: 2 Hrs)

Study various stages of mitosis using permanent slides

http://cbii-au.vlabs.ac.in/cell-biology-2/Mitosis_in_Onion_Root_Tips/

Session 44 (Practice 11: 2 Hrs)

Study various stages of meiosis using permanent slides

https://www.youtube.com/watch?v=hJttpMnSqiU

Session 50

Activation of gene transcription by GPCR

Video: https://www.youtube.com/watch?v=0OUn9188fM8&t=23s

Session 52 (Practice 12: 2 Hrs)

Our Main Teachers

Sujit Kumar Mishra is working as Assistant Professor in the School of Applied Sciences, Centurion University of Technology and Management, Bolangir Campus, Odisha, India. He has completed his M. Sc. from School of Life Sciences, Sambalpur University, submitted his Ph. D. thesis in Sambalpur University, and awaiting award of the doctoral degree. He has awarded […]