Clinical Bacteriology
Teacher
Dr. Monali Priyadarsini Mishra
Category
Core Courses
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Course Name : CUTM1722-Clinical Bacteriology
Code(Credit) : (3-1-0)
Course Objectives
- To confirm the suspicion of infectious bacterial disease.
- To identify the etiologic agent by isolating the causative bacterial pathogen.
Learning Outcomes
- To learn the diagnosis of bacteria from infective regions of the body
- Study of their sensitivity
- Examination of bacteria from common samples.
- Study of control measures for nosocomial infection.
- Student can safeguard himself & society and can work diagnostics and hospitals
Course Syllabus
Module-1
Laboratory strategy in the diagnosis of various Infective syndromes: Samples of choice, Collection, transportation and processing of samples for laboratory diagnosis of the following complications:
a) Septicemia and bacteraemia, b) Upper Respiratory tract infections, c) Lower Respiratory tract infections d) Wound, skin, and deep sepsis, e) Urinary tract infections, f) Genital Tract infections, g) Meningitis, h) Gastro intestinal infections, i) Enteric fever, j) Tuberculosis (Pulmonary and Extra- pulmonary), k) Pyrexia of unknown origin
Practice: Collection of nasal swab and its culture
Isolation of microorganism from wound
Module-2
Antibiotic susceptibility testing in bacteriology- a. Definition of antibiotics, b. Culture medium used for Antibiotic susceptibility testing, c. Preparation and standardization of inoculum, d. Control bacterial strains, e. Choice of antibiotics, f. MIC and MBC, g. Various methods of Antibiotic susceptibility testing with special reference to Stokes method and Kirby-Bauer method, h. Tests for production of β-lactamase
Practice: Preparation and use of different media in bacteriology laboratory
Antimicrobial susceptibility testing
Module-3
Bacteriological examination of water, milk, food and air –
- Examination of water - Collection and transportation of water sample, Presumptive coliform count, Eijkman test, Introduction and importance of other bacteria considered as indicators of faecal contamination
- Examination of Milk and milk products - Basic Concepts regarding gradation of milk, Various tests for Bacteriological examination
- Examination of food articles -Basic Concepts regarding classification of food like frozen food, canned food, raw food, cooked food etc. Various tests for Bacteriological examination with special reference to food poisoning bacteria
- Examination of Air -Significance of air bacteriology in healthcare facilities, Collection processing and reporting of an airsample.
Practice: Isolation of microorganisms from frozen food
Isolation of microorganisms from water
Suggested References:
- Practical Medical Microbiology by Mackie & MacCartney Volume 1 and2
- Text book of Microbiology by Ananthanereyan
- Medical Microbiology by Paniker & SatishGupte
- Medical Microbiology-by Fritz H. Kayser et al
- Bailey and Scott's Diagnostic Microbiology(12th) Ed
- Medical laboratory Technology vol.I, II, III by Mukherjee
- Medical Laboratory manual for tropical countries Vol II Microbiology by MoniaCheesbrough
Session Plan
Session 1
Laboratory strategy in the diagnosis of various Infective syndromes: Samples of choice, Collection
Video: https://www.youtube.com/watch?v=cIddzUYzUI4
Video: https://www.youtube.com/watch?v=C7W1WdXq-fI
Presentation: https://www.slideshare.net/YESANNA/saple-collection
Session 2
Transportation and processing of samples for laboratory diagnosis
Video: https://www.youtube.com/watch?v=XpnqWdzxyBc
Presentation: https://www.slideshare.net/jit12/collection-and-transport
Session 3
Septicemia and bacteraemia
Video: https://www.youtube.com/watch?v=VWm971N6u1c
Presentation:https://www.slideshare.net/AshishSaxena12/septicemia-11536070
Video:https://www.youtube.com/watch?v=HZ4aSoAxaaM
Presentation: https://www.slideshare.net/OmarAnsari14/bacteremia-121256365
Session 4
Upper Respiratory tract infections
Video:https://www.youtube.com/watch?v=AmmOJ3MtKHg
Presentation: https://www.slideshare.net/E_neutron/upper-respiratory-tract-infection-urti
Session 5
Lower Respiratory tract infections
Video: https://www.youtube.com/watch?v=knwJOxuf9B8
Presentation : https://www.slideshare.net/manalihsolanki/lower-respiratory-tract-infections-ppt
Session 6
Wound, skin, and deep sepsis
Video: https://www.youtube.com/watch?v=eYAZvhZzyS4
Video: https://www.youtube.com/watch?v=4q4Okx980wI
Presentation: https://www.slideshare.net/rajeshrs927/wound-infections
Presentation: https://www.slideshare.net/sa3edbajafar/skin-and-wound-infection
Session 7
Urinary tract infections
Video: https://www.youtube.com/watch?v=lY2bZjggc08
Presentation: https://www.slideshare.net/bindupulugurtha/urinary-tract-infections-11817974
Session 8
Genital Tract infections
Video: https://www.youtube.com/watch?v=66zR_FypVFQ
Presentation: https://www.slideshare.net/SharonAntony8/genital-tract-infections
Session 9
Meningitis
Video: https://www.youtube.com/watch?v=gIHUJs2eTHA
Presentation: https://www.slideshare.net/Maheshkumar1029/meningitis-71600507
Session 10
Gastrointestinal infections
Video: https://www.youtube.com/watch?v=QZhvJc7PCVI
Video: https://www.youtube.com/watch?v=hscFXP-aQN8
Presentation: https://www.slideshare.net/pathologybasics/gastrointestinal-infections-bacteriology
Session 11
Enteric fever
Video 1: https://www.youtube.com/watch?v=XkZTS8ep5wQ
Video 2: https://www.youtube.com/watch?v=xOamez79hLA&t=198s
Presentation:https://www.slideshare.net/yuyuricci/enteric-fever-typhoid-fever
Session 12
Tuberculosis (Pulmonary and Extra- pulmonary)
Video: https://www.youtube.com/watch?v=9v_LakTGQnA
Presentation: https://www.slideshare.net/ikramdr01/tuberculosis-173278862
Session 13
Pyrexia of unknown origin
Video: https://www.youtube.com/watch?v=NcV8Q5s2AnI
Presentation: https://www.slideshare.net/yuyuricci/pyrexia-of-unknown-origin-puo-93465734
Practice:
Collection of nasal swab and its culture
Session 14
Antibiotic susceptibility testing in bacteriology- a. Definition of antibiotics
Video: https://www.youtube.com/watch?v=OqQxtFcXNGk
Presentation: https://www.slideshare.net/InNoCeNtLiErPk/antibiotics-57427881
Session 15
Culture medium used for Antibiotic susceptibility testing
Video: https://www.youtube.com/watch?v=XhyMUHVbrlI&t=125s
Presentation: https://www.slideshare.net/doctorrao/antibiotic-sensitivity-testing-presentation
Session 16
Preparation and standardization of inoculum
Video: https://www.youtube.com/watch?v=M6KpdQjsgdI
Presentation: https://www.slideshare.net/arshgull/act-antimicrobial-suceptibility-testing-inocuulation-and-drug-sensitivity
Session 17
Control bacterial strains, Choice of antibiotics, MIC and MBC
Video: https://www.coursera.org/learn/antimicrobial-resistance
Presentation: https://www.slideshare.net/doctorrao/minimum-inhibitory-concentration
Session 18
Various methods of Antibiotic susceptibility testing with special reference to Stokes method and Kirby-Bauer method
Video: https://www.youtube.com/watch?v=BXr_kcki4Ag
Video: https://www.coursera.org/learn/antimicrobial-resistance
Presentation: https://www.slideshare.net/doctorrao/antibiotic-sensitivity-testing-presentation
Session 19
Tests for production of β-lactamase
Video: https://www.youtube.com/watch?v=qBdYnRhdWcQ
Presentation: https://www.slideshare.net/TaoufikDjerboua/beta-lactamases-structure-classification-and-investigations
Practice:
Preparation and use of different media in bacteriology laboratory
Practice:
Antimicrobial susceptibility testing
Session 20
Bacteriological examination of water, milk, food and air
Video: https://www.youtube.com/watch?v=WFUhI5X_kJw
Presentation: https://www.slideshare.net/drakmane/bacteriology-of-water-milk-and-air
Session 21
Examination of water - Collection and transportation of water sample
Video: https://www.youtube.com/watch?v=lzaAmeXKlqA
Video: https://www.youtube.com/watch?v=qoSvbbId18o
Presentation: https://www.slideshare.net/mirzafarullah/water-sampling-procedure-purpose-techniques-and-equipments
Session 22
Presumptive coliform count
Video: https://www.youtube.com/watch?v=i0433lTS908
Presentation: https://www.slideshare.net/prakashtu/bacteriological-analysis-of-drinking-water-by-mpn-method
Session 23
Eijkman test, Introduction and importance of other bacteria considered as indicators of faecal contamination
Video: https://www.youtube.com/watch?v=B1AVxzccS5Q
Video: https://www.youtube.com/watch?v=LpDjGCsmxZI
Presentation: https://www.slideshare.net/MariyaRaju/bacteriological-analysis-of-drinking-water
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC543678&blobtype=pdf
Session 24
Examination of Milk and milk products - Basic Concepts regarding gradation of milk
Video: https://www.youtube.com/watch?v=1qXGrCs21rk
Presentation: https://www.slideshare.net/YNarayudu/analysis-of-milk
Session 25
Various tests for Bacteriological examination of Milk
Video: https://www.youtube.com/watch?v=4hi6GYxhPNQ
Presentation: https://www.slideshare.net/gladysjanefranken/milk-microbiology
Session 26
Examination of food articles -Basic Concepts regarding classification of food like frozen food, canned food, raw food, cooked food etc
Video: https://www.youtube.com/watch?v=WLaijPw50W8
Presentation: https://www.slideshare.net/HiwrHastear/microbiological-examination-of-food
Session 27
Various tests for Bacteriological examination with special reference to food poisoning bacteria
Video: https://www.youtube.com/watch?v=as_kBbcW3iE
Presentation: https://www.slideshare.net/anchalawana/rapid-methods-of-detection-of-food-borne-pathogens
https://www.slideshare.net/nagaa/microbiologicalexaminationmethodsoffoodandwateralaboratorymanual
Session 28
Examination of Air -Significance of air bacteriology in healthcare facilities
Video: https://www.youtube.com/watch?v=Na3fJoz2Ytg&t=36s
https://www.youtube.com/watch?v=s1sKkwWA7zM
Presentation: http://www.brainkart.com/article/Bacteriology-of-Air_18628/
Session 29
Collection processing and reporting of an airsample.
Video: https://www.youtube.com/watch?v=Na3fJoz2Ytg&t=36s
https://www.youtube.com/watch?v=s1sKkwWA7zM
Presentation: http://www.brainkart.com/article/Bacteriology-of-Air_18628/
Practice:
Isolation of microorganisms from frozen food
Case Studies
Case Studies
Our Main Teachers
Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]