Clinical Bacteriology

Teacher

Dr. Monali Priyadarsini Mishra

Category

Core Courses

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Course Name : Clinical Bacteriology

Code(Credit) : (3-1-0)

Course Objectives

  • To confirm the suspicion of infectious bacterial disease.
  • To identify the etiologic agent by isolating the causative bacterial pathogen.

Learning Outcomes

  • To learn the diagnosis of bacteria from infective regions of the body
  • Study of their sensitivity
  • Examination of bacteria from common samples.
  • Study of control measures for nosocomial infection.
  • Student can safeguard himself & society and can work diagnostics and hospitals

Course Syllabus

Module-1

Laboratory strategy in the diagnosis of various Infective syndromes: Samples of choice, Collection, transportation and processing of samples for laboratory diagnosis of the following complications:

a) Septicemia and bacteraemia, b) Upper Respiratory tract infections, c) Lower Respiratory tract infections d) Wound, skin, and deep sepsis, e) Urinary tract infections, f) Genital Tract infections, g) Meningitis, h) Gastro intestinal infections, i) Enteric fever, j) Tuberculosis (Pulmonary and Extra- pulmonary), k) Pyrexia of unknown origin

Practice: Collection of nasal swab and its culture

                 Isolation of microorganism from wound

Module-2 

Antibiotic susceptibility testing in bacteriology- a. Definition of antibiotics, b. Culture medium used for Antibiotic susceptibility testing, c. Preparation and standardization of inoculum, d. Control bacterial strains, e. Choice of antibiotics, f. MIC and MBC, g. Various methods of Antibiotic susceptibility testing with special reference to Stokes method and Kirby-Bauer method, h. Tests for production of β-lactamase

Practice: Preparation and use of different media in bacteriology laboratory

                 Antimicrobial susceptibility testing

Module-3 

Bacteriological examination of water, milk, food and air –

  1. Examination of water - Collection and transportation of water sample, Presumptive coliform count, Eijkman test, Introduction and importance of other bacteria considered as indicators of faecal contamination
  2. Examination of Milk and milk products - Basic Concepts regarding gradation of milk, Various tests for Bacteriological examination
  3. Examination of food articles -Basic Concepts regarding classification of food like frozen food, canned food, raw food, cooked food etc. Various tests for Bacteriological examination with special reference to food poisoning bacteria
  4. Examination of Air -Significance of air bacteriology in healthcare facilities, Collection processing and reporting of an airsample.

Practice: Isolation of microorganisms from frozen food

                Isolation of microorganisms from water

Suggested Readings:

  • Practical Medical Microbiology by Mackie & MacCartney Volume 1 and2
  • Text book of Microbiology by Ananthanereyan
  • Medical Microbiology by Paniker & SatishGupte
  • Medical Microbiology-by Fritz H. Kayser et al
  • Bailey and Scott's Diagnostic Microbiology(12th) Ed
  • Medical laboratory Technology vol.I, II, III by Mukherjee
  • Medical Laboratory manual for tropical countries Vol II Microbiology by MoniaCheesbrough

Session Plan

Session 1

Laboratory strategy in the diagnosis of various Infective syndromes: Samples of choice, Collection

Video: https://www.youtube.com/watch?v=cIddzUYzUI4

Video: https://www.youtube.com/watch?v=C7W1WdXq-fI

Presentation: https://www.slideshare.net/YESANNA/saple-collection

Session 2

Transportation and processing of samples for laboratory diagnosis

Video: https://www.youtube.com/watch?v=XpnqWdzxyBc

Presentation: https://www.slideshare.net/jit12/collection-and-transport

Session 3

Session 4

Session 5

Session 6

Session 7

Session 8

Session 9

Session 10

Session 11

Session 12

Tuberculosis (Pulmonary and Extra- pulmonary)

Video: https://www.youtube.com/watch?v=9v_LakTGQnA

Presentation: https://www.slideshare.net/ikramdr01/tuberculosis-173278862

Session 13

Practice:

Collection of nasal swab and its culture

Video: https://www.youtube.com/watch?v=1H759HE7y50

Practice:

Isolation of microorganism from wound

Video: https://www.youtube.com/watch?v=A42aaHz9wm8

Video: https://www.youtube.com/watch?v=6jbVcMgN_HI

Session 14

Antibiotic susceptibility testing in bacteriology- a. Definition of antibiotics

Video: https://www.youtube.com/watch?v=OqQxtFcXNGk

Presentation: https://www.slideshare.net/InNoCeNtLiErPk/antibiotics-57427881

Session 15

Session 16

Session 17

Control bacterial strains, Choice of antibiotics, MIC and MBC

Video: https://www.coursera.org/learn/antimicrobial-resistance

Presentation: https://www.slideshare.net/doctorrao/minimum-inhibitory-concentration

Session 18

Various methods of Antibiotic susceptibility testing with special reference to Stokes method and Kirby-Bauer method

Video: https://www.youtube.com/watch?v=BXr_kcki4Ag

Video: https://www.coursera.org/learn/antimicrobial-resistance

Presentation: https://www.slideshare.net/doctorrao/antibiotic-sensitivity-testing-presentation

Session 19

Practice:

Preparation and use of different media in bacteriology laboratory

Video: https://www.youtube.com/watch?v=NhnOsvmv2AY

Practice:

Antimicrobial susceptibility testing

Video: https://www.youtube.com/watch?v=XhyMUHVbrlI&t=28s

Session 20

Bacteriological examination of water, milk, food and air

Video: https://www.youtube.com/watch?v=WFUhI5X_kJw

Presentation: https://www.slideshare.net/drakmane/bacteriology-of-water-milk-and-air

Session 21

Session 22

Session 23

Session 24

Examination of Milk and milk products - Basic Concepts regarding gradation of milk

Video: https://www.youtube.com/watch?v=1qXGrCs21rk

Presentation: https://www.slideshare.net/YNarayudu/analysis-of-milk

Session 25

Various tests for Bacteriological examination of Milk

Video: https://www.youtube.com/watch?v=4hi6GYxhPNQ

Presentation: https://www.slideshare.net/gladysjanefranken/milk-microbiology

Session 26

Examination of food articles -Basic Concepts regarding classification of food like frozen food, canned food, raw food, cooked food etc

Video: https://www.youtube.com/watch?v=WLaijPw50W8

Presentation: https://www.slideshare.net/HiwrHastear/microbiological-examination-of-food

Session 27

Session 28

Examination of Air -Significance of air bacteriology in healthcare facilities

Video: https://www.youtube.com/watch?v=Na3fJoz2Ytg&t=36s

https://www.youtube.com/watch?v=s1sKkwWA7zM

Presentation: http://www.brainkart.com/article/Bacteriology-of-Air_18628/

Session 29

Practice:

Isolation of microorganisms from frozen food

Video: https://www.youtube.com/watch?v=BlKP35bct2o

Practice:

Isolation of microorganisms from water

Video: https://www.youtube.com/watch?v=6Y6wrH9oCMY

Case Studies

Case Studies

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    Our Main Teachers

    Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]