Course Objectives

• Understanding the concept of Biochemical analyzing instruments, chemicals and normal ranges of biochemical components in our body.
• Clinically relevant biochemical analysis for deeper understanding of all biochemical components i.e., Proteins, Electrolytes, Hormones etc

Learning Outcomes

• To learn about tests carried out for biochemical investigations.
• Understanding of principle of biochemical Clinical biochemistry tests.
• To learn normal ranges and abnormal ranges of biochemical components and hormones.
• To study about diseases related to biochemical and hormone imbalance in human body.

Course Syllabus

Module-1 ( 10 Hrs)

LFT, KFT, Lipid profile, Estimation of Glucose, Hormone test: T3, T4, TSH, Prolactin, 17 Kitosteroids

Practice: Demonstration the centrifuge machine, Demonstration of Colorimeter, Method of estimation and assessment for: a. Glucose tolerance test , Detection of sugar in Urine, Estimation of Protein in urine, Estimation of  Liver function test, Kidney function test, Lipid profile, Thyroid

Module-2 ( 10 Hrs)

Metabolic disorders and Diagnostic enzymology: Disorders of metabolism: carbohydrate, Lipids, Amino acids and Nucleic acids. Diagnostic enzymes: Role of Enzymes in Clinical Practice: Marker enzymes in myocardium, liver and pancreas. Tumor markers, Radio isotope techniques

Module-3 ( 14 Hrs)

Organ function tests: Liver function tests, Bile pigment metabolism, tests for liver function. Jaundice and its type, Functions of Kidney, Urine formation and renal function tests disease of kidney , Renal Calculi : Theory of formation and analysis , Gastric Analysis , Composition of gastric juice, concepts of free and bound acid, Fractional Test Meal

Practice: Operation procedure of Centrifuge machine, colorimeter etc. Estimation of Liver function test, Kidney function test, Estimation of bile pigment, bile salt, bilirubin etc.

Suggested Readings

1. Text book of Medical Laboratory Technology by P. B. Godker, PublisherBhalani.
2. Text book of Medical Biochemistry by Chaterjee & Shinde, PublisherJPB
3. Medical Laboratory Technology by Mukherjee
4. Principal of Biochemistry by Lehninger, Publisher Kalyani
5. Practical Clinical Biochemistry by Harold Varley, Publisher CBS. 
6. Ebook link-https://www.cartercenter.org/resources/pdfs/health/ephti/library/lecture_notes/health_science_students/medicalbiochemistry.pdf
7. Ebook link-https://books.google.co.in/books?id=Je_pJfb2r0cC&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
8. Ebook link-https://books.google.co.in/books?id=csPcDAAAQBAJ&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
9. Ebook link-https://books.google.co.in/books?id=2FkXAwAAQBAJ&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false

All the practice components can be performed by simulation as well as virtual labs.

Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).

Online Source: Udemy, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia,slideshare.net, Researchget.net etc.

Session Plan

Session 1

Topic: LFT

Study Materials: 

Notes-https://www.healthline.com/health/liver-function-tests

https://www.javatpoint.com/lft-full-form

Session 2

Topic: KFT

Study Materials:

Notes-https://www.healthline.com/health/kidney-function-tests

https://www.labpedia.net/renal-function-tests-renal-parameters-part-1/

Session 3

Topic: Lipid Profile

Study Materials: 

Notes-https://www.portea.com/labs/diagnostic-tests/lipid-profile-69/

https://my.clevelandclinic.org/health/diagnostics/17176-lipid-blood-tests

Session 4

Topic: Method of estimation and assessment for: a. Glucose tolerance test

Study Materials:

Notes-https://www.healthline.com/health/glucose-tolerance-test

Session 5

Topic: T3

Study Materials: 

Notes-https://www.healthline.com/health/t3

https://medlineplus.gov/lab-tests/triiodothyronine-t3-tests/

https://labtestsonline.org/tests/t3-free-and-total

https://medlineplus.gov/ency/article/003687.htm

Session 6

Topic: T4

Study Materials: 

Notes-https://www.healthline.com/health/t4-test

https://medlineplus.gov/lab-tests/thyroxine-t4-test/

https://labtestsonline.org/tests/t4-free

Session 7

Topic: TSH

Study Materials: 

Notes-https://medlineplus.gov/lab-tests/tsh-thyroid-stimulating-hormone-test/#:~:text=TSH%20stands%20for%20thyroid%20stimulating,way%20your%20body%20uses%20energy.

https://www.webmd.com/women/what-is-tsh-test#1

https://www.healthline.com/health/tsh

https://labtestsonline.org/tests/thyroid-stimulating-hormone-tsh

https://www.medicalnewstoday.com/articles/326774

https://www.yourhormones.info/hormones/thyroid-stimulating-hormone/

Session 8

Topic: Prolactin & Hyperprolactinemia

Study Materials: 

Notes-https://www.healthline.com/health/prolactin#:~:text=Prolactin%20is%20produced%20by%20the,men%20is%20not%20well%2Dknown.

https://www.webmd.com/a-to-z-guides/prolactin-test#1

https://www.medicalnewstoday.com/articles/322279

https://medlineplus.gov/lab-tests/prolactin-levels/

https://www.hormone.org/your-health-and-hormones/glands-and-hormones-a-to-z/hormones/prolactin

Hyperprolactinemia- https://www.reproductivefacts.org/news-and-publications/patient-fact-sheets-and-booklets/documents/fact-sheets-and-info-booklets/hyperprolactinemia-high-prolactin-levels/

Session 9

Topic: 17 Kitosteroid & Kitosteroids urine test

Study Materials: 

Notes-https://www.ucsfbenioffchildrens.org/tests/003460.html

https://medlineplus.gov/ency/article/003460.htm

https://www.ucsfhealth.org/medical-tests/003460

http://pennstatehershey.adam.com/content.aspx?productid=117&pid=1&gid=003460

Session 10

Practice session:

Topic: Glucose Tolerance Test

Study Materials:

Video- https://www.youtube.com/watch?v=qnduyqPuCP4

https://www.youtube.com/watch?v=zBOyi5Y4LmM

Session 11

Practice session:

Topic: Protein in Urine

Study Materials:

Video- https://www.youtube.com/watch?v=i7dZBzhQCaI

Description- https://www.kidneyfund.org/kidney-disease/kidney-problems/protein-in-urine.html#:~:text=The%20test%20for%20protein%20in,a%20sign%20of%20kidney%20disease

Notes- https://www.healthline.com/health/urine-protein-test#test-procedures

Session 12

Practice session:

Study Materials:

Topic: Detection of Sugar in Urine

Video-https://www.youtube.com/watch?v=-eg-MHdoJ_Q

Session 13

Topic: Disorders of metabolism of carbohydrate

Study Materials: 

Notes-https://www.britannica.com/science/metabolic-disease/Disorders-of-carbohydrate-metabolism

Session 14

Topic: Disorders of metabolism of Lipids

Study Materials: 

Notes-https://www.britannica.com/science/metabolic-disease/Disorders-of-lipid-metabolism

Session 15

Topic: Disorders of metabolism of Amino acids

Study Materials: 

Notes-https://www.britannica.com/science/metabolic-disease/Disorders-of-amino-acid-metabolism

Session 16

Topic: Disorders of metabolism of Nucleic acids

Study Materials: 

Notes-https://www.slideshare.net/AthiraDas2/disorders-of-nucleic-acid-metabolism

Session 17

Topic: Role of Enzymes in Clinical Practice of Marker enzymes in myocardium

Study Materials: 

Notes-https://www.urmc.rochester.edu/encyclopedia/content.aspx?contenttypeid=167&contentid=cardiac_biomarkers

Session 18

Topic: Role of Enzymes in Clinical Practice of Marker enzymes in liver

Study Materials: 

Notes-https://www.healthline.com/health/enzyme-markers

Session 19

Topic: Role of Enzymes in Clinical Practice of Marker enzymes in Pancreas

Study Materials:

Notes-https://www.healthline.com/health/enzyme-markers

Session 20

Topic: Tumor Markers

Study Materials: 

Notes- https://www.cancer.gov/about-cancer/diagnosis-staging/diagnosis/tumor-markers-fact-sheet

https://www.cancer.net/navigating-cancer-care/diagnosing-cancer/tests-and-procedures/tumor-marker-tests#:~:text=A%20tumor%20marker%20is%20a,markers%20are%20also%20called%20biomarkers.

https://labtestsonline.org/tests/tumor-markers

https://medlineplus.gov/lab-tests/tumor-marker-tests/

Session 21

Topic: Radio isotope techniques

Study Materials:

Slide share- https://www.slideshare.net/abhigiri02/radioisotope-technique-and-methods

Session 22

Topic: Organ Function Tests

Study Materials:

Slide share- https://www.slideshare.net/PradeepNarwat/organ-function-tests

Video- https://study.com/academy/topic/clinical-test-results-organ-function-and-health.html

Session 23

Topic: Liver function tests

Study Materials:

Notes- https://www.healthline.com/health/liver-function-tests

Session 24

Topic: Bile pigment metabolism

Study Materials:

PDF- https://www.annualreviews.org/doi/pdf/10.1146/annurev.me.12.020161.001235

Slide share- https://www.slideshare.net/MDSohrabAliMollah/bile-pigments

Session 25

Topic: Jaundice and its type

Study Materials:

Notes- https://teachmesurgery.com/hpb/presentations/jaundice/

https://www.healthline.com/health/jaundice-types

https://www.medlife.com/blog/jaundice-symptoms-treatment-causes-types/

Session 26

Topic: Functions of Kidney: Urine formation, renal function tests & disease of kidney

Study Materials: 

Notes- https://www.healthline.com/health/kidney-function-tests

https://www.onlinebiologynotes.com/physiology-urine-formation/

http://visiblebody.com/learn/urinary/urine-creation#:~:text=The%20kidneys%20filter%20unwanted%20substances,are%20removed%20from%20the%20body.

https://www.mayoclinic.org/diseases-conditions/chronic-kidney-disease/symptoms-causes/syc-20354521#:~:text=Chronic%20kidney%20disease%2C%20also%20called,then%20excreted%20in%20your%20urine.

https://www.webmd.com/a-to-z-guides/understanding-kidney-disease-basic-information#1

Session 27

Topic: Renal Calculi : Theory of formation and analysis

Study Materials:

Notes- https://www.healthline.com/health/kidney-

https://www.mayoclinic.org/diseases-conditions/kidney-stones/symptoms-causes/syc-20355755

https://www.kidneyfund.org/kidney-disease/kidney-problems/kidney-stones/

https://www.medicinenet.com/kidney_stones/article.htm

https://www.mountsinai.org/health-library/diseases-conditions/kidney-stones

https://www.medicalnewstoday.com/articles/154193

https://pmpediatrics.com/dear-dr-christina/renal-calculus-im-not-talking-about-math/

Session 28

Topic: Composition of gastric juice

Study Materials:

Slide share-https://www.slideshare.net/silajet/gastric-juice

https://slideplayer.com/slide/12266070/

Session 29

Topic: Gastric Juice collection & Analysis

Study Materials:

Video- https://www.youtube.com/watch?v=Abf3Gd6AaZQ

Notes- https://healthcarentsickcare.com/gastric-functions-gastric-function-test

https://www.encyclopedia.com/medicine/encyclopedias-almanacs-transcripts-and-maps/gastric-analysis-0#:~:text=Gastric%20analysis%20consists%20of%20a,that%20stimulates%20gastric%20acid%20output

Slide share- https://www.slideshare.net/krishreenamacato/i-gastric-fluid-analysis

Session 30

Topic: Fractional Test Meal

Study Materials:

Notes- https://www.encyclopedia.com/education/dictionaries-thesauruses-pictures-and-press-releases/fractional-test-meal

Session 31

Practice session:

Topic: Detection of Bile Salt 

Study Materials:

Video- https://www.youtube.com/watch?v=xcJ31KS8U6M

https://www.youtube.com/watch?v=qW8_6TxICS4

Session 32

Practice session:

Topic: Detection of Bile Pigment in urine

Study Materials:

Video- https://www.youtube.com/watch?v=ac8gXAvHSPo

Case Studies

Case Studies