Clinical Biochemistry

Teacher

Susmita Chakrabarty

Category

Core Courses

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Course Name : Clinical Biochemistry

Code(Credit) : CUTM1718 (2-1-0)

Course Objectives

  • Understanding the concept of Biochemical analyzing instruments, chemicals and normal ranges of biochemical components in our body.
  • Clinically relevant biochemical analysis for deeper understanding of all biochemical components i.e., Proteins, Electrolytes, Hormones etc

Learning Outcomes

  • To learn about tests carried out for biochemical investigations.
  • Understanding of principle of biochemical Clinical biochemistry tests.
  • To learn normal ranges and abnormal ranges of biochemical components and hormones.
  • To study about diseases related to biochemical and hormone imbalance in human body.

Course Syllabus

Module-1 ( 10 Hrs)

 

LFT, KFT, Lipid profile, Estimation of Glucose, Hormone test: T3, T4, TSH, Prolactin, 17 Kitosteroids

 

Practice: Demonstration the centrifuge machine, Demonstration of Colorimeter, Method of estimation and assessment for: a. Glucose tolerance test , Detection of sugar in Urine, Estimation of Protein in urine, Estimation of  Liver function test, Kidney function test, Lipid profile, Thyroid

 

Module-2 ( 10 Hrs)

 

Metabolic disorders and Diagnostic enzymology: Disorders of metabolism: carbohydrate, Lipids, Amino acids and Nucleic acids. Diagnostic enzymes: Role of Enzymes in Clinical Practice: Marker enzymes in myocardium, liver and pancreas. Tumor markers, Radio isotope techniques

 

Module-3 ( 14 Hrs)

 

Organ function tests: Liver function tests, Bile pigment metabolism, tests for liver function. Jaundice and its type, Functions of Kidney, Urine formation and renal function tests disease of kidney , Renal Calculi : Theory of formation and analysis , Gastric Analysis , Composition of gastric juice, concepts of free and bound acid, Fractional Test Meal

 

Practice: Operation procedure of Centrifuge machine, colorimeter etc. Estimation of Liver function test, Kidney function test, Estimation of bile pigment, bile salt, bilirubin etc.

 

Suggested Readings

 

  1. Text book of Medical Laboratory Technology by P. B. Godker, PublisherBhalani.
  2. Text book of Medical Biochemistry by Chaterjee & Shinde, PublisherJPB
  3. Medical Laboratory Technology by Mukherjee
  4. Principal of Biochemistry by Lehninger, Publisher Kalyani
  5. Practical Clinical Biochemistry by Harold Varley, Publisher CBS. 
  6. Ebook link-https://www.cartercenter.org/resources/pdfs/health/ephti/library/lecture_notes/health_science_students/medicalbiochemistry.pdf
  7. Ebook link-https://books.google.co.in/books?id=Je_pJfb2r0cC&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
  8. Ebook link-https://books.google.co.in/books?id=csPcDAAAQBAJ&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false
  9. Ebook link-https://books.google.co.in/books?id=2FkXAwAAQBAJ&printsec=frontcover&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false

 

 

All the practice components can be performed by simulation as well as virtual labs.

Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).

Online Source: Udemy, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia,slideshare.net, Researchget.net etc.

 

Session 4

Topic: Method of estimation and assessment for: a. Glucose tolerance test

Study Materials:

Notes-https://www.healthline.com/health/glucose-tolerance-test

Session 10

Practice session:

Topic: Glucose Tolerance Test

Study Materials:

Video- https://www.youtube.com/watch?v=qnduyqPuCP4

https://www.youtube.com/watch?v=zBOyi5Y4LmM

Session 12

Practice session:

Study Materials:

Topic: Detection of Sugar in Urine

Video-https://www.youtube.com/watch?v=-eg-MHdoJ_Q

Session 13

Topic: Disorders of metabolism of carbohydrate

Study Materials: 

Notes-https://www.britannica.com/science/metabolic-disease/Disorders-of-carbohydrate-metabolism

Session 14

Topic: Disorders of metabolism of Lipids

Study Materials: 

Notes-https://www.britannica.com/science/metabolic-disease/Disorders-of-lipid-metabolism

Session 15

Topic: Disorders of metabolism of Amino acids

Study Materials: 

Notes-https://www.britannica.com/science/metabolic-disease/Disorders-of-amino-acid-metabolism

Session 16

Topic: Disorders of metabolism of Nucleic acids

Study Materials: 

Notes-https://www.slideshare.net/AthiraDas2/disorders-of-nucleic-acid-metabolism

Session 17

Topic: Role of Enzymes in Clinical Practice of Marker enzymes in myocardium

Study Materials: 

Notes-https://www.urmc.rochester.edu/encyclopedia/content.aspx?contenttypeid=167&contentid=cardiac_biomarkers

Session 18

Topic: Role of Enzymes in Clinical Practice of Marker enzymes in liver

Study Materials: 

Notes-https://www.healthline.com/health/enzyme-markers

Session 19

Topic: Role of Enzymes in Clinical Practice of Marker enzymes in Pancreas

Study Materials:

Notes-https://www.healthline.com/health/enzyme-markers

Session 21

Topic: Radio isotope techniques

Study Materials:

Slide share- https://www.slideshare.net/abhigiri02/radioisotope-technique-and-methods

Session 23

Topic: Liver function tests

Study Materials:

Notes- https://www.healthline.com/health/liver-function-tests

Session 28

Topic: Composition of gastric juice

Study Materials:

Slide share-https://www.slideshare.net/silajet/gastric-juice

https://slideplayer.com/slide/12266070/

Session 31

Practice session:

Topic: Detection of Bile Salt 

Study Materials:

Video- https://www.youtube.com/watch?v=xcJ31KS8U6M

https://www.youtube.com/watch?v=qW8_6TxICS4

Session 32

Practice session:

Topic: Detection of Bile Pigment in urine

Study Materials:

Video- https://www.youtube.com/watch?v=ac8gXAvHSPo

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      Miss Susmita Chakrabarty is currently working as a Lecturer in School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes field in Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique & Clinical Biochemistry etc. In her research, […]