Clinical Hematology
Teacher
Susmita Chakrabarty
Category
Core Courses
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Course Name : Clinical Hematology
Code(Credit) : CUTM1712 (3-1-0)
Course Objectives
- The Clinical Hematology course will cover the diagnosis and management of blood cell disorders, anatomy and physiology of hematopoiesis, routine specialized hematology tests, analysis, classification, and monitoring of blood cell abnormalities.
- Clinically relevant hematological analysis for deeper understanding evaluate normal and abnormal cell morphology with associated diseases and other blood components .
- Be able to handle an investigation of hematological disorder and laboratory abnormalities such as anaemia, polycythemia, leukopenia, leukocytosis, thrombocytopenia, thrombocytosis, elevated ESR etc within hematology.
Learning Outcomes
- Differentiate various hematological procedures and the use of basic equipment essential to working in a Hematology Laboratory.
- Discuss differences between Quality control, Quality Assurance, and Continuing Quality Improvement principles as used in the Hematology Laboratory.
- Categorize various hematology analyses , operational principles of various hematology instruments, and troubleshooting of various instruments.
- Explain the principles and theories utilized in a variety of problem-solving situations.
- Define testing suitability standards for Hematology specimens.
- To be able to carry out blood sampling & Evaluate specimen acceptability.
Course Syllabus
Module-1 (16 Hrs)
Scope & importance of Haematology, important equipment and chemicals, various test performed in Haematology laboratory. Identify and/or confirm the composition of various red blood cell inclusions. Function of normal cellular components ,Formation of blood, Erythropoiesis, thrombopoiesis .Anticoagulants, definition, Uses, Different types, mode of action, their merits and demerits. Morphology of normal blood cells, abnormal morphology & diseases, Hematological Disorder
Practice: Demonstration of instruments used in hematology- Microscope, Blood Cell counter. Demonstration of different blood cell, their synthesis from slide presentation or chart. Demonstration the normal and abnormal morphology of different blood cells.
Module-2 (18 Hrs)
Collection and preservation of blood: different methods of collection(venous and capillary blood), preservation, changes in stored blood normal and absolutely values in hematology,Preparation of peripheral blood film (PBF), To stain a peripheral blood Film by Leishman stain, Malaria thick smear preparation, Different types of stains, Romanwsky stains: principle of staining, Hemoglobin estimation (oxy Hb and cyanmethaemoglobinmethod), Complete Blood Cell Count: Total RBC count, Total WBC count, Platelet count, DLC value, HB, MCH, MCV, MCHC, Determination of ESR by wintrobes, Determination of ESR by Westergren’s method, Determination of PCV by Wintrobes, Reticulocyte Count, Absolute Eosinophil Count, Morphology of Red Blood Cells.
Practice: Different methods of collection(venous and capillary blood), Preparation of DLC, TLC, TRBC etc. Estimation of ESR, Complete blood cell count, Blood grouping, Hb and values of MCH, MCV, MCHC, PCV, Staining & Smear preparation.
Module-3 (14 Hrs)
Hematological Disorders
- Classification of Anemia: Morphological &etiological.
- Iron Deficiency Anemia: Distribution of body Iron, IronAbsorption, causes of iron deficiency, labfindingsc. Megaloblastic Anemia: Causes, Labfindings.
- Hemolytic Anemia: Definition, causes, classification & labfindings.
- Laboratory diagnosis of Hemophilia and von-will brand disease.
- Laboratory diagnosis of Idiopathic thrombocytopenic purpura (ITP),
- Platelet function tests and their interpretation.
Practice: Observation about different normal and abnormal morphology of RBCs, WBCs, Platelet, Bleeding Time & Clotting Time, PT & APTT.
Suggested Readings:
- Textbook of Medical Laboratory Technology P.B Gotkar Mumbai, Bhalani Publishing House
- Text book of Medical Laboratory Technology by Paraful B. Godkar, Publisher Bhalani
- Text book of Medical Laboratory Technology (2nd edition) by V.H Talib, Publisher CBS
- Atlas of hematology (5th edition) by G.A. McDonald, Publisher Churchill Livingstone
- Medical Laboratory Technology By K.L Mukharjee, Publisher McGraw Hill education pvtlimited
- Text book of Medical Laboratory Technology (6th edition) by Ramnik Sood,Jaypee Publication.
- Ebook link-https://www.pdfdrive.com/hematology-basic-principles-and-practice-e176384006.html
- Ebook link-https://www.pdfdrive.com/hematology-basic-principles-and-practice-expert-consult-online-and-print-expert-consult-title-online-print-5th-edition-e186195241.html
- Ebook link-https://books.google.co.in/books?id=6sfacydDNsUC&printsec=frontcover&dq=hematology&hl=en&sa=X&ved=2ahUKEwja9-ve3I7qAhUwzTgGHSMUDekQ6wEwAHoECAQQAQ#v=onepage&q=hematology&f=false
- Ebook link-https://books.google.co.in/books?id=QQcYAAAAYAAJ&printsec=frontcover&dq=hematology&hl=en&sa=X&ved=2ahUKEwja9-ve3I7qAhUwzTgGHSMUDekQ6wEwAnoECAIQAQ#v=onepage&q=hematology&f=false
Simulation links for labs:
All the practice components can be performed by simulation as well as virtual labs. Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg), labsforlife (http://labsforlife.in/)
Online Source: Udemy, Coursera, article library, NCBI,Study.com, You tube, Slide share, Frontier, Febs, unacademy, academia, MedlinePlus, LabsforLife.
Session Plan
Session 1
Topic: Scope & importance of Haematology
Study Materials:
Video- https://www.youtube.com/watch?v=3VlckXrY9TU
https://www.youtube.com/watch?v=FcGZH93xubs
Discussion- https://www.ilshospitals.com/blog/scope-of-haematology/
Session 2
Topic: Hematological important equipment and chemicals
Study Materials:
Session 3
Topic: various test performed in Haematology laboratory
Study Materials:
Discussion- https://study.com/academy/lesson/what-is-a-hematology-test-common-tests-interpretations.html
https://www.hopkinsmedicine.org/health/treatment-tests-and-therapies/hematology
Slideshare- https://www.slideshare.net/hillainemarie/hematology-laboratory-tests
Session 4
Topic: Composition and function of blood
Study Materials:
Video- https://www.youtube.com/watch?v=qrE6Y0Se8bw&pbjreload=101
https://www.youtube.com/watch?v=VSVYgivfs9c
Slideshare- https://www.slideshare.net/BijiSaro/blood-composition-and-its-functions-on-17092016
Session 5
Topic: Function of normal cellular components
Study Materials:
Video- https://www.youtube.com/watch?v=yj7bfZKlIp8
Discussion- https://www.khanacademy.org/science/biology/human-biology/circulatory-pulmonary/a/components-of-the-blood
Session 6
Session 7
Topic: Erythropoiesis
Study Materials:
Video- https://www.youtube.com/watch?v=YEt2D8xM9b0
https://www.youtube.com/watch?v=aE4d7JS-TTQ
https://www.coursera.org/lecture/immunologyfundamentalsimmunitybcells/hematopoiesis-ooWGI
Session 10
Topic: Anticoagulants: definition, Types, Uses
Study Materials:
Video- https://www.youtube.com/watch?v=jrWD83b8hkA
https://www.youtube.com/watch?v=-OksYhAt5c4
Slide share- https://www.slideshare.net/JananiMathialagan1/blood-collection-and-anticoagulants
Session 11
Topic: Anticoagulants: mode of action
Study Materials:
Video- https://www.youtube.com/watch?v=eZBtQ0rDnG4
Slide Share- https://www.slideshare.net/peddanasunilkumar/anticoagulants-used-in-haematology
Session 12
Topic: Anticoagulants: merits and demerits
Study Materials:
Slide Share- https://www.slideshare.net/peddanasunilkumar/anticoagulants-used-in-haematology
Session 13
Topic: Morphology of normal blood cells & abnormal morphology & diseases
Study Materials:
Video- https://www.youtube.com/watch?v=6PITMN4lil4
https://www.youtube.com/watch?v=z3k3GZ1F_rg
https://www.youtube.com/watch?v=z3k3GZ1F_rg&t=222s
Discussion- https://www.labpedia.net/red-blood-cell-rbc-part-1-morphology-peripheral-blood-smear-normal-picture/
Session 14
Topic: Hematological Disorders
Study Materials:
Video- https://www.youtube.com/watch?v=06gtqiIWd0I
https://www.youtube.com/watch?v=RD8gmKRcvJs
Notes- https://theoncologyinstitute.com/hematologic-disorders/
Session 15
Topic: Collection and preservation of blood: different methods of collection
Study Materials:
Slide share- https://www.slideshare.net/globalsoin/blood-collection-and-preservation
Session 16
Practice Session:
Topic: Collection and preservation of blood: capillary blood collection
Study Materials:
Session 17
Practice Session:
Study Materials:
Topic: Collection and preservation of blood: Venous Blood collection
Session 18
Practice Session:
Topic: Blood Grouping and Rh typing
Study Materials:
Session 19
Practice Session:
Topic: Preparation of peripheral blood film (PBF)
Study Materials:
Video- https://www.youtube.com/watch?v=cI9GObT73lY
https://www.youtube.com/watch?v=ihSnTKLUazQ
Session 20
Practice Session:
Topic: To stain a peripheral blood Film by Leishman- stain
Study Materials:
Session 21
Practice Session:
Topic: Maleria Thick smear preparation
Study Materials:
Session 22
Practice Session:
Topic: Smear preparation , Staining & Different types of stains used in Hematological Laboratory
Study Materials:
Slide share- https://www.slideshare.net/DrSHAHIDRaza/special-and-routine-stains-in-haematology-1
Session 23
Topic: Romanwsky stains: principle of staining
Study Materials:
Procedure- https://www.vetstream.com/treat/lapis/technique/staining-technique-romanowsky-stains-overview
Slide share- https://www.slideshare.net/nawakharneupane/dyes-and-stain
Session 24
Practice Session:
Topic: Haemoglobin estimation (Sahali's method)
Study Materials:
Video- https://www.youtube.com/watch?v=qMTJ05l855Q
Session 25
Practice Session:
Topic: Haemoglobin estimation (cyanmethaemoglobin method)
Study Materials:
Session 26
Practice Session:
Topic: Complete Blood Counts
Study Materials:
Video- https://www.youtube.com/watch?v=j7PJrUFciec
https://www.youtube.com/watch?v=4DCCm5o92q8&t=21s
https://www.youtube.com/watch?v=20x2AQNZUfQ&t=155s
https://www.youtube.com/watch?v=W2RxJVIc0EI&t=195s
https://www.youtube.com/watch?v=7kqpOz5VfU0
Slide share- https://www.slideshare.net/drgauhar1/cbc-43258873
Session 27
Practice Session:
Topic: TRBC Count by Haemocytometers
Study Materials:
Video- https://www.youtube.com/watch?v=JzGW9PovzGg
Session 28
Practice Session:
Topic: TLC by Haemocytometer
Study Materials:
Session 29
Practice Session:
Topic: Differential Leukocyte count
Study Materials:
Session 30
Practice Session:
Topic: Determination of Platelet Count
Study Materials:
Session 31
Topic: Erythrocyte Indices- MCV, MCH, MCHC
Study Materials:
Video- https://www.youtube.com/watch?v=Uz1zSNZI2H0
Session 32
Practice Session:
Topic: Determination of ESR by wintrobes
Study Materials:
Session 33
Practice Session:
Topic: Determination of ESR by Westergeren's method
Study Materials:
Session 34
Practice Session:
Topic: Determination of PCV by Wintrobes
Study Materials:
Session 35
Practice Session:
Topic: Reticulocyte Count
Study Materials:
Session 36
Practice Session:
Topic: Absolute Eosinophil Count
Study Materials:
Session 37
Topic: Classification of Anemia: Morphological & etiological
Study Materials:
Session 38
Topic: Distribution & Absorption of Iron in body
Study Materials:
Video- https://www.youtube.com/watch?v=y1HGudIjkVg
Slide share- https://www.slideshare.net/nikhilgupta129/iron-physiology
Session 39
Topic: Iron Deficiency Anemia: Causes, Symptoms & Lab Diagnosis
Study Materials:
Video- https://www.youtube.com/watch?v=WvD4p8FkQpY&t=462s
Session 40
Topic: Megaloblastic Anemia: Causes, symptoms & Lab Diagnosis
Study Materials:
Session 41
Topic: Hemolytic Anemia: Definition, causes, classification & labfinding
Study Materials:
Session 42
Topic: Laboratory diagnosis of Hemophilia and von-will brand disease
Study Materials:
Discussion- https://hemophilianewstoday.com/hemophilia-testing-and-screening/
https://www.hemophilia.org/Bleeding-Disorders/Types-of-Bleeding-Disorders/Von-Willebrand-Disease
Session 43
Topic: Laboratory diagnosis of Idiopathic thrombocytopenic purpura (ITP)
Study Materials:
Article- https://emedicine.medscape.com/article/202158-overview
Session 44
Topic: Platelet function tests and their interpretation
Study Materials:
Discussion- https://labtestsonline.org/tests/platelet-function-tests
Session 45
Practice Session:
Topic: Bleeding time & clotting time
Study Materials:
Video- https://www.youtube.com/watch?v=bMVy6pCWhRk
https://www.youtube.com/watch?v=AYipSViRduE
Session 46
Practice session:
Topic: Prothrombin Time & Active Partial Prothrombin time
Study Materials:
https://www.webmd.com/a-to-z-guides/partial-thromboplastin-time-test#1
https://medlineplus.gov/lab-tests/partial-thromboplastin-time-ptt-test/
https://www.healthline.com/health/partial-thromboplastin-time-ptt
https://www.medicine.mcgill.ca/physio/vlab/bloodlab/PT_PTT.htm
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Our Main Teachers
Susmita Chakrabarty is currently working as an Assistant Professor in the School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes the field of Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique, Analytical Biochemistry & Clinical Biochemistry, etc. […]