Clinical Hematology

Teacher

Susmita Chakrabarty

Category

Core Courses

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Course Name : Clinical Hematology

Code(Credit) : CUTM1712 (3-1-0)

Course Objectives

  • The Clinical Hematology course will cover the diagnosis and management of blood cell disorders, anatomy and physiology of hematopoiesis, routine specialized hematology tests, analysis, classification, and monitoring of blood cell abnormalities.
  • Clinically relevant hematological analysis for deeper understanding evaluate normal and abnormal cell morphology with associated diseases and other blood components .
  • Be able to handle an investigation of hematological disorder and laboratory abnormalities such as anaemia, polycythemia, leukopenia, leukocytosis, thrombocytopenia, thrombocytosis, elevated ESR etc within hematology.

Learning Outcomes

  • Differentiate various hematological procedures and the use of basic equipment essential to working in a Hematology Laboratory.
  • Discuss differences between Quality control, Quality Assurance, and Continuing Quality Improvement principles as used in the Hematology Laboratory.
  • Categorize various hematology analyses , operational principles of various hematology instruments, and troubleshooting of various instruments.
  • Explain the principles and theories utilized in a variety of problem-solving situations.
  • Define testing suitability standards for Hematology specimens.
  • To be able to carry out blood sampling & Evaluate specimen acceptability.

Course Syllabus

Module-1 (16 Hrs)

 

Scope & importance of Haematology, important equipment and chemicals, various test performed in Haematology laboratory. Identify and/or confirm the composition of various red blood cell inclusions. Function of normal cellular components ,Formation of blood, Erythropoiesis, thrombopoiesis .Anticoagulants, definition, Uses, Different types, mode of action, their merits and demerits. Morphology of normal blood cells, abnormal morphology & diseases, Hematological Disorder

 

Practice: Demonstration of instruments used in hematology- Microscope, Blood Cell counter. Demonstration of different blood cell, their synthesis from slide presentation or chart. Demonstration the normal and abnormal morphology of different blood cells.

 

 

Module-2 (18 Hrs)

 

Collection and preservation of blood: different methods of collection(venous and capillary blood), preservation, changes in stored blood normal and absolutely values in hematology,Preparation of peripheral blood film (PBF), To stain a peripheral blood Film by Leishman stain, Malaria thick smear preparation, Different types of stains, Romanwsky stains: principle of staining, Hemoglobin estimation (oxy Hb and cyanmethaemoglobinmethod), Complete Blood Cell Count: Total RBC count, Total WBC count, Platelet count, DLC value, HB, MCH, MCV, MCHC, Determination of ESR by wintrobes, Determination of ESR by Westergren’s method, Determination of PCV by Wintrobes,  Reticulocyte Count, Absolute Eosinophil Count, Morphology of Red Blood Cells.

 

Practice: Different methods of collection(venous and capillary blood), Preparation of DLC, TLC, TRBC etc. Estimation of ESR, Complete blood cell count, Blood grouping, Hb and values of MCH, MCV, MCHC, PCV, Staining & Smear preparation.

 

Module-3 (14 Hrs)

 

Hematological Disorders

  1. Classification of Anemia: Morphological &etiological.
  2. Iron Deficiency Anemia: Distribution of body Iron, IronAbsorption, causes of iron deficiency, labfindingsc. Megaloblastic Anemia: Causes, Labfindings.
  3. Hemolytic Anemia: Definition, causes, classification & labfindings.
  4. Laboratory diagnosis of Hemophilia and von-will brand disease.
  5. Laboratory diagnosis of Idiopathic thrombocytopenic purpura (ITP),
  6. Platelet function tests and their interpretation.

 

Practice: Observation about different normal and abnormal morphology of RBCs, WBCs, Platelet, Bleeding Time & Clotting Time, PT & APTT.

 

Suggested Readings:

  1. Textbook of Medical Laboratory Technology P.B Gotkar Mumbai, Bhalani Publishing House
  2. Text book of Medical Laboratory Technology by Paraful B. Godkar, Publisher Bhalani
  3. Text book of Medical Laboratory Technology (2nd edition) by V.H Talib, Publisher CBS
  4. Atlas of hematology (5th edition) by G.A. McDonald, Publisher Churchill Livingstone
  5. Medical Laboratory Technology By K.L Mukharjee, Publisher McGraw Hill education pvtlimited
  6. Text book of Medical Laboratory Technology (6th edition) by Ramnik Sood,Jaypee Publication.
  7. Ebook link-https://www.pdfdrive.com/hematology-basic-principles-and-practice-e176384006.html
  8. Ebook link-https://www.pdfdrive.com/hematology-basic-principles-and-practice-expert-consult-online-and-print-expert-consult-title-online-print-5th-edition-e186195241.html
  9. Ebook link-https://books.google.co.in/books?id=6sfacydDNsUC&printsec=frontcover&dq=hematology&hl=en&sa=X&ved=2ahUKEwja9-ve3I7qAhUwzTgGHSMUDekQ6wEwAHoECAQQAQ#v=onepage&q=hematology&f=false
  10. Ebook link-https://books.google.co.in/books?id=QQcYAAAAYAAJ&printsec=frontcover&dq=hematology&hl=en&sa=X&ved=2ahUKEwja9-ve3I7qAhUwzTgGHSMUDekQ6wEwAnoECAIQAQ#v=onepage&q=hematology&f=false

 

Simulation links for labs: 

All the practice components can be performed by simulation as well as virtual labs. Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg), labsforlife (http://labsforlife.in/)

Online Source: Udemy, Coursera, article library, NCBI,Study.com, You tube, Slide share, Frontier, Febs, unacademy, academia, MedlinePlus, LabsforLife.

Session 2

Session 3

Topic: various test performed in Haematology laboratory

Study Materials:

Discussion- https://study.com/academy/lesson/what-is-a-hematology-test-common-tests-interpretations.html

https://www.hopkinsmedicine.org/health/treatment-tests-and-therapies/hematology

Slideshare- https://www.slideshare.net/hillainemarie/hematology-laboratory-tests

Session 9

Session 11

Session 12

Topic: Anticoagulants:  merits and demerits

Study Materials:

Slide Share- https://www.slideshare.net/peddanasunilkumar/anticoagulants-used-in-haematology

Session 15

Topic: Collection and preservation of blood: different methods of collection

Study Materials:

Slide share- https://www.slideshare.net/globalsoin/blood-collection-and-preservation

Session 16

Practice Session:

Topic: Collection and preservation of blood: capillary blood collection

Study Materials:

Video- https://www.youtube.com/watch?v=8-NVVSOBUu8

https://www.youtube.com/watch?v=ibU5PYOF2qg

Session 17

Practice Session:

Study Materials:

Topic: Collection and preservation of blood: Venous Blood collection

Video- https://www.youtube.com/watch?v=r8n2O1GIqQg

https://www.youtube.com/watch?v=7NSEFVbzTAU

Session 18

Practice Session:

Topic: Blood Grouping and Rh typing

Study Materials:

Video- https://www.youtube.com/watch?v=cKnEdvrmHK4

https://www.youtube.com/watch?v=Lx3CAmMpvCE

Session 20

Practice Session:

Topic: To stain a peripheral blood Film by Leishman- stain

Study Materials:

Video- https://www.youtube.com/watch?v=Aa0XvwGXsK8

Session 21

Practice Session:

Topic: Maleria Thick smear preparation

Study Materials:

Video- https://www.youtube.com/watch?v=WPP7AjmStBg

Session 22

Practice Session:

Topic: Smear preparation , Staining & Different types of stains used in Hematological Laboratory

Study Materials:

Slide share- https://www.slideshare.net/DrSHAHIDRaza/special-and-routine-stains-in-haematology-1

https://www.slideshare.net/NechirZaxoyi/stain-of-hematology

Session 23

Session 25

Practice Session:

Topic: Haemoglobin estimation (cyanmethaemoglobin method)

Study Materials:

Video- https://www.youtube.com/watch?v=deydnUQdhzA&t=66s

https://www.youtube.com/watch?v=deydnUQdhzA

Session 27

Session 28

Practice Session:

Topic: TLC by Haemocytometer

Study Materials:

Video- https://www.youtube.com/watch?v=MKS0KM3lr90

https://www.youtube.com/watch?v=ItZIgFRRyqw

Session 29

Practice Session:

Topic: Differential Leukocyte count

Study Materials:

Video- https://www.youtube.com/watch?v=u-pykuHqQmU

https://www.youtube.com/watch?v=NMVFvY3znBA

Session 30

Practice Session:

Topic: Determination of Platelet Count

Study Materials:

Video- https://www.youtube.com/watch?v=Qf5nXANq6o0

https://www.youtube.com/watch?v=Qf5nXANq6o0&t=172s

Session 32

Practice Session:

Topic: Determination of ESR by wintrobes

Study Materials:

Video- https://www.youtube.com/watch?v=QhfK_A6ebC8

https://www.youtube.com/watch?v=QhfK_A6ebC8&t=180s

Session 33

Practice Session:

Topic: Determination of ESR by Westergeren's method

Study Materials:

Video- https://www.youtube.com/watch?v=gy8RpErVsqc&t=164s

https://www.youtube.com/watch?v=gy8RpErVsqc

Session 34

Practice Session:

Topic: Determination of PCV by Wintrobes

Study Materials:

Video- https://www.youtube.com/watch?v=DfG6C0gKjhU

https://www.youtube.com/watch?v=RoS3w_Sng_Q

Session 35

Practice Session:

Topic: Reticulocyte Count

Study Materials:

Video- https://www.youtube.com/watch?v=5ipo6IPhKR8

https://www.youtube.com/watch?v=Od_kaiVCwTM

Session 36

Practice Session:

Topic: Absolute Eosinophil Count

Study Materials:

Video- https://www.youtube.com/watch?v=g5x2rflR1WM

https://www.youtube.com/watch?v=Sb6tSb_JqNI

Session 37

Topic: Classification of Anemia: Morphological & etiological

Study Materials:

Video- https://www.youtube.com/watch?v=t6Kh0dSWamo

https://www.youtube.com/watch?v=Vh1LZlLCfkU

Session 38

Topic: Distribution & Absorption of  Iron in body

Study Materials:

Video- https://www.youtube.com/watch?v=y1HGudIjkVg

Slide share- https://www.slideshare.net/nikhilgupta129/iron-physiology

Session 39

Session 43

Session 44

Topic: Platelet function tests and their interpretation

Study Materials:

Discussion- https://labtestsonline.org/tests/platelet-function-tests

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    Susmita Chakrabarty is currently working as a Lecturer in the School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes the field of Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique, Analytical Biochemistry & Clinical Biochemistry, etc. She […]