Clinical Pathology
Teacher
Prof. Sunil Kumar Jha
Category
Core Courses
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Course Name : Clinical Pathology
Code(Credit) :CUTM1715 (3-1-0)
Course Objectives
- Analyze body fluid for diagnosis of disease
- Analyze waste product for diagnosis of disease
- Understanding DOT Policy
- Understand Physiological disorder and infectious disease
- Analysis of pregnancy
Course Outcomes
| COs | Course Outcomes | Mapping COs with POs (High-3, Medium-2, Low- 1) |
| CO1 | Able to collect pathological specimen | PO2 (3) |
| CO2 | Able to detect physiological disorders like diabetes, ketosis, nephritis, jaundice and infectious diseases (UTI, Hematuria, Filaria, Dysentery, Ulcer, TB, etc.) | PO1 (3), PO6(2) |
| CO3 | Understands the method of Preservation and processing of pathological sample. | PO4 (2) |
| CO4 | Analyse the Infertility disorder | PO5 (2) |
Course Syllabus
Module-1 (16 Hrs)
Introduction of clinical pathology, Composition, collection and preservation of urine , Physical examination of Urine,Chemical Examination of Urine - Sugar and Ketone bodies, Diabetes and Ketosis, Nephritis and UTI, Albumin, Phosphate, BJP, Bile Salt and Bile pigment,Chemical Examination of Urine - Multistix reagent strip, Jaundice, Microscopical Examination of Urine, Operation of Urine Analyzer, Pregnancy test, Report writing and report analysis of Urine
Practice: Operation of Urine analyzer, Benedict Test, Heat and Acid Test, Rothera’s Test, Benzidine Test, Fouchet’s Test
Lab :-
Urine Analysis: Collection and Physical Examination, Specific Gravity, Benedict's Qualitative test, Acetone Rothera's Test, Protein and BJP Test, Hay's Test and Fouchet's test, Benzidine test, Microscopical Examination, Pregnancy Test, Auto-mentation by Urine analyzer
Module-2 (14 Hrs)
Respiratory Tract Infection: Gram Staining and ZN Staining, Basic of DOT Centre, Report writing and report analysis of sputum, Sputum for the diagnosis of Mycobacterium tuberculosis,Clinical significance and Report writing of Stool, Difference between Amoebic, Dysentery and Bacillary Dysentery, Microscopical Examination of Stool, Physical and Chemical examination of Stool, Composition, collection and preservation of stool
Practice: Microscopic finding of stool, Morphology of stool parasite
Lab :-
Stool Analysis: Collection and physical examination, Chemical Examination, Occult test and reducing sugar, Microscopical Examination: Protozoa, Microscopical Examination: Helminthes
Sputum Analysis: Collection and physical examination, Tuberculosis (ZN Stain), Respiratory infection (Gram Stain)
Module-3 (15 Hrs)
Routine laboratory investigation of Pleural Fluid, Routine laboratory investigation of Pericardial Fluid, Routine laboratory investigation of Synovial Fluid, Synovial fluid: Collection and preservation, Examination of CSF related to Meningitis, Brain Tumour and other disorder, CSF: Composition, Collection, Preservation and physical examination, Report analysis and report writing of Semen, Semen examination for male infertility disorder, Semen: Composition, function, collection and physical examination
Practice: Gram stain, ZN Stain, General consideration on specimen collection
Lab :-
Semen Analysis: Collection and physical examination, Chemical Examination, Microscopical examination
CSF Analysis: Collection and Routine Examination
Synovial Fluid: Collection and Routine examination
Pleural Fluid: Collection and routine examination
Pericardial Fluid: Collection and routine examination
Bacteriological Examination of throat swab
Suggested Readings:
- Textbook of Clinical laboratory methods and diagnosis by Gradwohl's, Publisher Mosby
- Medical laboratory technology Vol.1 by K. L. Mukherjee, 2007, Publisher Tata McGraw Hill
- Textbook of medical laboratory technology by Praful B Godkar, Publisher Balan
- Medical laboratory science theory and practice by J Ochei and Kolhatkar, 2002, Tata McGraw- Hill, Publisher TBS
Session Plan
Session 1
Topic : Introduction of clinical pathology
Book Reference : Godkar, Pg 1-6
Video : https://www.youtube.com/watch?v=M8IrduJ4Lyw
Presentation : https://www.slideshare.net/VarugheseGeorge/urine-analysis-193584532
Session 2
Topic : Composition, collection and preservation of urine
Book Reference : Mukherjee Pg 801-808
Video : https://www.youtube.com/watch?v=vPvuWZ1Uhb8
Presentation : https://www.slideshare.net/DrSayanDas1/examination-of-urine-125445216
Session 3
Topic : Physical examination of Urine
Book Reference : Mukherjee Pg 808-813
Video : https://www.youtube.com/watch?v=MbVOM5urrRo
Presentation : https://www.slideshare.net/onlyuforu3/urine-analysis-25156109
Session 4
Practice session
Topic : Urine Analysis : Collection and Physical Examination
Video : https://www.youtube.com/watch?v=d8w5SICzzxc
Presentation : https://www.slideshare.net/DrSayanDas1/examination-of-urine-125445216
Virtual Lab : urinalysis_virtual_lab_1
Session 5
Practice session
Topic : Urine Analysis : Specific Gravity
Session 6
Topic : Chemical Examination of Urine - Sugar and Ketone bodies
Book Reference : Godkar, Pg 112,113,786,797,799
Video : https://www.youtube.com/watch?v=-atHARq0JbQ
Presentation : https://www.slideshare.net/TapeshwarYadav1/ketone-bodies-56014473
Session 7
Practice session
Topic : Urine Analysis : Benedict's Qualitative test
Video : https://www.youtube.com/watch?v=-atHARq0JbQ
Presentation : https://www.slideshare.net/mobile/drjayeshpatidar/examination-of-urine
Virtual Lab : https://amrita.olabs.edu.in/?sub=79&brch=17&sim=207&cnt=615
Session 8
Practice session
Topic : Urine Analysis : Acetone Rothera's Test
Video : https://www.youtube.com/watch?v=7tcdQ5FkFX0
Presentation : https://www.slideshare.net/manojmahato9638/urine-acetone
Session 9
Topic : Diabetes and Ketosis
Book Reference : Godkar Pg 275-278, Talib Pg 48-49
Video : https://www.youtube.com/watch?v=yIc2XFN
Presentation : https://www.slideshare.net/AkinbiOlubayodeo/diabetes-ketoacidosis-65475156
Session 10
Topic : Albumin, Phosphate, BJP
Book Reference : Talib Pg 47-57
Video : https://www.youtube.com/watch?v=vfua4bB1Btg
Presentation : https://medlineplus.gov/lab-tests/phosphate-in-urine/
Session 11
Practice session
Topic : Urine Analysis : Protein and BJP Test
Video : https://www.youtube.com/watch?v=7tcdQ5FkFX0
Virtual lab : https://amrita.olabs.edu.in/?sub=79&brch=17&sim=208&cnt=2
Session 12
Topic : Nephritis and UTI
Book Reference : Godkar Pg 1433-1436, Talib Pg 47-57
Video: https://www.youtube.com/watch?v=Htv2x7Dp9dc
Presentation : https://www.healthline.com/health/acute-nephritic-syndrome
Session 13
Practice Session
Topic : Urine Analysis : Benzidine test
Video : https://www.youtube.com/watch?v=nWbPhP4OYiM
Presentation : https://www.slideshare.net/swatiwadhai/urine-examination
Session 14
Topic : Bile Salt and Bile pigment
Book Reference : Godkar Pg 1437-1439
Video : https://www.youtube.com/watch?v=xcJ31KS8U6M
Presentation : https://www.slideshare.net/charukharbanda1/bile
Session 15
Practice session
Topic : Urine Analysis : Hay's Test and Fouchet's test
Video : https://www.youtube.com/watch?v=xcJ31KS8U6M
Presentation : https://www.slideshare.net/charukharbanda1/bile
Virtual lab : https://www.labpedia.net/bile-salts-in-urine
Session 16
Topic : Chemical Examination of Urine - Multistix reagent strip
Book Reference : Godkar Pg 1444-1445
Video : https://www.youtube.com/watch?v=7nAU-G8Cyjo
Presentation : https://www.slideshare.net/amerali6/pregnancy-test-55779960
Session 17
Topic : Jaundice
Reference : Godkar Pg 420-423
Video : https://www.youtube.com/watch?v=tpBsmTd4rNo
Presentation : https://www.slideshare.net/KirshaSkishore/jaundice-92845731
Session 18
Topic : Microscopical Examination of Urine
Book Reference : Godakar 1146-1451
Video : https://www.youtube.com/watch?v=VrsU0Ki26hM
Presentation : https://www.slideshare.net/debbarma1989/microscopic-examination-of-urine-and-other-special-tests
Session 19
Practice session
Topic : Urine Analysis : Microscopical Examination
Video : https://www.youtube.com/watch?v=22MHdz5sEuc
Presentation :
Virtual Lab : http://vlab.amrita.edu/?sub=3&brch=76&sim=214&cnt=1
Session 20
Topic : Operation of Urine Analyzer
Book Reference : Operation Manual of Instrument
Video : https://www.youtube.com/watch?v=6TCIOB1vUvI
Presentation : https://www.slideshare.net/manjunathatm/automated-urine-analysis-71543720
Session 21
Topic : Pregnancy test, Report writing and report analysis of Urine
Reference : Operation Manual of Kits
Book Reference : Godkar Pg 224
Session 22
Practice session
Topic : Urine Analysis : Pregnancy Test
Video : https://www.youtube.com/watch?v=fPGwMm3amkU
Presentation : https://www.slideshare.net/amerali6/pregnancy-test-55779960
Session 23
Topic : Composition, collection and preservation of stool
Book Reference : Talib Pg 70-79
Video : https://www.youtube.com/watch?v=gtnPYZWfnSU
Presentation : https://www.slideshare.net/drsudeesh/stool-examination
Session 24
Topic : Physical and Chemical examination of Stool
Book Reference : Talib Pg 70-79
Video : https://www.youtube.com/watch?v=NEAmF0Qnyik
Presentation : https://www.slideshare.net/drsudeesh/stool-examination
Session 25
Practice session
Topic : Stool Analysis : Collection and physical examination
Video : https://www.youtube.com/watch?v=-iI2PxmHxuo&t
Presentation : https://www.slideshare.net/drsudeesh/stool-examination
Session 26
Topic : Microscopical Examination of Stool
Book Reference : Talib Pg 70-79
Video : https://www.youtube.com/watch?v=_ePqcdDKCe0
Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool
Session 27
Practice session
Topic : Stool Analysis Microscopic Examination : Protozoa
Video : https://www.youtube.com/watch?v=MRzUXg8kFiY
Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool
Session 28
Practice Session
Topic : Stool Analysis Microscopic Examination : Helminthes
Video : https://www.youtube.com/watch?v=H6mCLzpLPz0
Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool
Session 29
Practice session
Topic : Stool Analysis : Chemical Examination
Video : https://www.youtube.com/watch?v=-iI2PxmHxuo&t
Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool
Session 30
Practice session
Topic : Stool Analysis : Occult test and reducing sugar
Video : https://www.youtube.com/watch?v=Mqus-OtJTYI
Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool
Session 31
Topic : Difference between Amoebic Dysentery and Bacillary Dysentery
Book Reference : Mukherjee Pg 880-885
Video : https://www.youtube.com/watch?v=DIH5XB5UK5k
Presentation : https://www.slideshare.net/NishaPaudel1/differences-between-amoebic-and-bacillary-dysentery
Session 32
Topic : Clinical significance and Report writing of Stool
Book Reference : Mukherjee Pg 880-885
Video : https://www.youtube.com/watch?v=-iI2PxmHxuo
Presentation : https://www.slideshare.net/odairjoub/stool-analysis-84232092
Session 33
Topic : Sputum for the diagnosis of Mycobacterium tuberculosis
Book Reference : Godkar Pg 1539-1543
Video : https://www.youtube.com/watch?v=wY7ErwZlk48
Presentation : https://www.slideshare.net/AlfredJoel/sputum-examination-and-analysis
Session 34
Practice Session
Topic :Sputum Analysis : Collection and physical examination
Video : https://www.youtube.com/watch?v=FH9eiWp0TZ4
Presentation : https://www.slideshare.net/AlfredJoel/sputum-examination-and-analysis
Session 35
Topic : Basic of DOT Centre, Report writing and report analysis of sputum
Video : https://www.youtube.com/watch?v=dSMQJ_UbeK4
Presentation : https://www.slideshare.net/AlfredJoel/sputum-examination-and-analysis
Session 36
Topic : Respiratory Tract Infection: Gram Staining and ZN Staining
Book Reference : Godkar Pg 1539-1543
Video : https://www.youtube.com/watch?v=L1x6LoL7pTw
Presentation : https://www.slideshare.net/shanthoshpriyan/rntcp-57543326
Session 37
Session Plan
Topic : Sputum Analysis : Tuberculosis(ZN Stain)
Video : https://www.youtube.com/watch?v=wY7ErwZlk48
Presentation : https://www.slideshare.net/shanthoshpriyan/rntcp-57543326
Session 38
Session Plan
Topic : Sputum Analysis : Respiratory infection(Gram Stain)
Video : https://www.youtube.com/watch?v=2MaZyId3XXY
Presentation : https://www.slideshare.net/shanthoshpriyan/rntcp-57543326
Session 39
Topic : Semen : Physical examination, Chemical Examination and Microscopical Examination
Book Reference : Mukherjee 871,879
Video : https://www.youtube.com/watch?v=0AK_ops65LI
Presentation : https://www.slideshare.net/drvipinDrvipinsharma/semen-analysis-91057379
Session 40
Practice Session
Topic : Semen Analysis : Collection and physical examination
Video : https://www.youtube.com/watch?v=0AK_ops65LI&t
Presentation : https://www.slideshare.net/drvipinDrvipinsharma/semen-analysis-91057379
Session 41
Practice Session
Topic :Semen Analysis : Chemical Examination
Video : https://www.youtube.com/watch?v=0AK_ops65LI&t
Presentation : https://www.slideshare.net/drvipinDrvipinsharma/semen-analysis-91057379
Session 42
Practice Session
Topic : Semen Analysis : Microscopical examination
Video : https://www.youtube.com/watch?v=wMcprAUe5Z0
Presentation : https://www.slideshare.net/drvipinDrvipinsharma/semen-analysis-91057379
Session 43
Topic : Report analysis and report writing of Semen
Book Reference : Tahlib Pg 66-67
Video : https://www.youtube.com/watch?v=Unt3EwbM-rA
Presentation : https://www.slideshare.net/danish29/semen-analysis-61887227
Session 44
Topic : CSF: Composition, Collection, Preservation and physical examination
Book Reference : Mukherjee Pg 850-857
Video : https://www.youtube.com/watch?v=LZqiu6PKrZU
Presentation : https://www.slideshare.net/UsmanAkbar4/csf-41592002
Session 45
Practice Sesion
Topic : CSF Analysis : Collection and Routine Examination
Video : https://www.youtube.com/watch?v=KSCFYLUJc7U&t
https://www.youtube.com/watch?v=LZqiu6PKrZU&t
Presentation : https://www.slideshare.net/UsmanAkbar4/csf-41592002
Session 46
Topic : Examination of CSF related to Meningitis, Brain Tumour and other disorder
Book Reference : Mukherjee Pg 865-869
Video : https://www.youtube.com/watch?v=gIHUJs2eTHA
Presentation : https://www.slideshare.net/bahoran03/meninges-and-related-tumors
Session 47
Topic : Synovial fluid : Collection and preservation
Book Reference : Godkar Pg 1544
Video : https://www.youtube.com/watch?v=0_fxPCQYUiA
Presentation : https://labtestsonline.org/tests/synovial-fluid-analysis
Session 48
Topic : Routine laboratory investigation of Synovial Fluid
Book Reference : Godkar Pg 1548-1550
Session 49
Practice Session
Topic : Synovial Fluid : Collection and Routine examination
Session 50
Topic : Routine laboratory investigation of Pericardial Fluid
Book Reference : Godkar Pg 1547-1548
Video : https://www.youtube.com/watch?v=wKYWhutqzyg
Presentation : https://www.slideshare.net/YESANNA/pericardial-fluid
Session 51
Practice Session
Topic : Pericardial Fluid : Collection and routine examination
Video : https://www.youtube.com/watch?v=ddahqoVGmIc&t
Presentation : https://www.slideshare.net/YESANNA/pericardial-fluid
Session 52
Topic : Routine laboratory investigation of Pleural Fluid
Video : https://www.youtube.com/watch?v=ddahqoVGmIc
Presentation : https://www.slideshare.net/nasirnazeer5/pleural-fluid-examination
Session 53
Practice Session
Topic : Pleural Fluid : Collection and routine examination
Video : https://www.youtube.com/watch?v=ddahqoVGmIc&t
Presentation : https://www.slideshare.net/nasirnazeer5/pleural-fluid-examination
Session 54
Practice session
Topic : Bacteriological Examination of throat swab
Video : https://www.youtube.com/watch?v=6jbVcMgN_HI
Presentation : https://www.slideshare.net/abeer-babeker/examination-of-throat
Virtual Lab : http://learn.chm.msu.edu/vibl/content/gramstain/gramstain/index.html
Our Main Teachers
Sunil Kumar Jha is working as the Dean of School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management since 2016. He has also taken the responsibility of Director of Community Diagnostic Center. His major areas of expertise are medical diagnostics , clinical pathology, diagnostic bacteriology, histopathology and patient safety. He […]