Clinical Pathology

Teacher

Prof. Sunil Kumar Jha

Category

Core Courses

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Course Name : Clinical Pathology

Code(Credit) :CUTM1715 (3-1-0)

 

Course Objectives

  • Analyze body fluid for diagnosis of disease
  • Analyze waste product for diagnosis of disease
  • Understanding DOT Policy
  • Understand Physiological disorder and infectious disease
  • Analysis of pregnancy

Course Outcomes

COs  Course Outcomes Mapping COs with POs (High-3, Medium-2, Low- 1)
CO1 Able to collect pathological specimen PO2 (3)
CO2 Able to detect physiological disorders like diabetes, ketosis, nephritis, jaundice and infectious diseases (UTI, Hematuria, Filaria, Dysentery, Ulcer, TB, etc.) PO1 (3), PO6(2)
CO3 Understands the method of Preservation and processing of pathological sample. PO4 (2)
CO4 Analyse the Infertility disorder PO5 (2)

Course Syllabus

Module-1 (16 Hrs)

 

Introduction of clinical pathology, Composition, collection and preservation of urine , Physical examination of Urine,Chemical Examination of Urine - Sugar and Ketone bodies, Diabetes and Ketosis, Nephritis and UTI, Albumin, Phosphate, BJP, Bile Salt and Bile pigment,Chemical Examination of Urine - Multistix reagent strip, Jaundice, Microscopical Examination of Urine, Operation of Urine Analyzer, Pregnancy test, Report writing and report analysis of Urine

Practice: Operation of Urine analyzer, Benedict Test, Heat and Acid Test, Rothera’s Test, Benzidine Test, Fouchet’s Test

Lab :-

Urine Analysis: Collection and Physical Examination, Specific Gravity, Benedict's Qualitative test, Acetone Rothera's Test, Protein and BJP Test, Hay's Test and Fouchet's test, Benzidine test, Microscopical Examination, Pregnancy Test, Auto-mentation by Urine analyzer

 

Module-2 (14 Hrs)

 

Respiratory Tract Infection: Gram Staining and ZN Staining, Basic of DOT Centre, Report writing and report analysis of sputum, Sputum for the diagnosis of Mycobacterium tuberculosis,Clinical significance and Report writing of Stool, Difference between Amoebic, Dysentery and Bacillary Dysentery, Microscopical Examination of Stool, Physical and Chemical examination of Stool, Composition, collection and preservation of stool

Practice: Microscopic finding of stool, Morphology of stool parasite

Lab :-

Stool Analysis: Collection and physical examination, Chemical Examination, Occult test and reducing sugar, Microscopical Examination: Protozoa, Microscopical Examination: Helminthes

Sputum Analysis: Collection and physical examination, Tuberculosis (ZN Stain), Respiratory infection (Gram Stain)

 

Module-3 (15 Hrs)

 

Routine laboratory investigation of Pleural Fluid, Routine laboratory investigation of Pericardial Fluid, Routine laboratory investigation of Synovial Fluid, Synovial fluid: Collection and preservation, Examination of CSF related to Meningitis, Brain Tumour and other disorder, CSF: Composition, Collection, Preservation and physical examination, Report analysis and report writing of Semen, Semen examination for male infertility disorder, Semen: Composition, function, collection and physical examination

Practice: Gram stain, ZN Stain, General consideration on specimen collection

Lab :- 

Semen Analysis: Collection and physical examination, Chemical Examination, Microscopical examination

CSF Analysis: Collection and Routine Examination

Synovial Fluid: Collection and Routine examination

Pleural Fluid: Collection and routine examination

Pericardial Fluid: Collection and routine examination

Bacteriological Examination of throat swab

 

Suggested Readings:

  1. Textbook of Clinical laboratory methods and diagnosis by Gradwohl's, Publisher Mosby
  2. Medical laboratory technology Vol.1 by K. L. Mukherjee, 2007, Publisher Tata McGraw Hill
  3. Textbook of medical laboratory technology by Praful B Godkar, Publisher Balan
  4. Medical laboratory science theory and practice by J Ochei and Kolhatkar, 2002, Tata McGraw- Hill, Publisher TBS

Session Plan

Session 1

Topic : Introduction of clinical pathology

Book Reference : Godkar, Pg 1-6

Video : https://www.youtube.com/watch?v=M8IrduJ4Lyw

Presentation : https://www.slideshare.net/VarugheseGeorge/urine-analysis-193584532

Session 2

Topic : Composition, collection and preservation of urine

Book Reference : Mukherjee Pg 801-808

Video : https://www.youtube.com/watch?v=vPvuWZ1Uhb8

Presentation : https://www.slideshare.net/DrSayanDas1/examination-of-urine-125445216

Session 3

Topic : Physical examination of Urine

Book Reference : Mukherjee Pg 808-813

Video : https://www.youtube.com/watch?v=MbVOM5urrRo

Presentation : https://www.slideshare.net/onlyuforu3/urine-analysis-25156109

Session 4

Practice session 

Topic : Urine Analysis : Collection and Physical Examination

Video : https://www.youtube.com/watch?v=d8w5SICzzxc

Presentation : https://www.slideshare.net/DrSayanDas1/examination-of-urine-125445216

Virtual Lab : urinalysis_virtual_lab_1

Session 5

Practice session 

Topic : Urine Analysis : Specific Gravity

Video : https://www.youtube.com/watch?v=-sRJ9zLmkc0

Session 6

Topic : Chemical Examination of Urine - Sugar and Ketone bodies

Book Reference : Godkar, Pg 112,113,786,797,799

Video : https://www.youtube.com/watch?v=-atHARq0JbQ

Presentation : https://www.slideshare.net/TapeshwarYadav1/ketone-bodies-56014473

Session 7

Session 8

Practice session

Topic : Urine Analysis : Acetone Rothera's Test

Video : https://www.youtube.com/watch?v=7tcdQ5FkFX0

Presentation : https://www.slideshare.net/manojmahato9638/urine-acetone

Session 9

Topic : Diabetes and Ketosis

Book Reference : Godkar Pg 275-278, Talib Pg 48-49

Video : https://www.youtube.com/watch?v=yIc2XFN

Presentation : https://www.slideshare.net/AkinbiOlubayodeo/diabetes-ketoacidosis-65475156

Session 10

Topic : Albumin, Phosphate, BJP

Book Reference : Talib Pg 47-57

Video : https://www.youtube.com/watch?v=vfua4bB1Btg

Presentation : https://medlineplus.gov/lab-tests/phosphate-in-urine/

Session 11

Practice session

Topic : Urine Analysis : Protein and BJP Test

Video : https://www.youtube.com/watch?v=7tcdQ5FkFX0

Virtual lab : https://amrita.olabs.edu.in/?sub=79&brch=17&sim=208&cnt=2

Session 12

Topic : Nephritis and UTI

Book Reference : Godkar Pg 1433-1436, Talib Pg 47-57

Video: https://www.youtube.com/watch?v=Htv2x7Dp9dc

Presentation : https://www.healthline.com/health/acute-nephritic-syndrome

Session 13

Practice Session

Topic : Urine Analysis : Benzidine test

Video : https://www.youtube.com/watch?v=nWbPhP4OYiM

Presentation : https://www.slideshare.net/swatiwadhai/urine-examination

Session 14

Topic : Bile Salt and Bile pigment

Book Reference : Godkar Pg 1437-1439

Video : https://www.youtube.com/watch?v=xcJ31KS8U6M

Presentation : https://www.slideshare.net/charukharbanda1/bile

Session 15

Practice session

Topic : Urine Analysis : Hay's Test and Fouchet's test

Video : https://www.youtube.com/watch?v=xcJ31KS8U6M

Presentation : https://www.slideshare.net/charukharbanda1/bile

Virtual lab : https://www.labpedia.net/bile-salts-in-urine

Session 16

Topic : Chemical Examination of Urine - Multistix reagent strip

Book Reference : Godkar Pg 1444-1445

Video : https://www.youtube.com/watch?v=7nAU-G8Cyjo

Presentation : https://www.slideshare.net/amerali6/pregnancy-test-55779960

Session 17

Topic : Jaundice

Reference : Godkar Pg 420-423

Video : https://www.youtube.com/watch?v=tpBsmTd4rNo

Presentation : https://www.slideshare.net/KirshaSkishore/jaundice-92845731

Session 18

Topic : Microscopical Examination of Urine

Book Reference : Godakar 1146-1451

Video : https://www.youtube.com/watch?v=VrsU0Ki26hM

Presentation : https://www.slideshare.net/debbarma1989/microscopic-examination-of-urine-and-other-special-tests

Session 19

Practice session

Topic : Urine Analysis : Microscopical Examination

Video : https://www.youtube.com/watch?v=22MHdz5sEuc

Presentation : 

Virtual Lab : http://vlab.amrita.edu/?sub=3&brch=76&sim=214&cnt=1

Session 20

Topic : Operation of Urine Analyzer

Book Reference : Operation Manual of Instrument

Video : https://www.youtube.com/watch?v=6TCIOB1vUvI

Presentation : https://www.slideshare.net/manjunathatm/automated-urine-analysis-71543720

Session 21

Topic : Pregnancy test, Report writing and report analysis of Urine

Reference : Operation Manual of Kits

Book Reference : Godkar Pg 224

Video : https://www.youtube.com/watch?v=Ohl2gwM5DyA

Session 22

Practice session

Topic : Urine Analysis : Pregnancy Test

Video : https://www.youtube.com/watch?v=fPGwMm3amkU

Presentation : https://www.slideshare.net/amerali6/pregnancy-test-55779960

Session 23

Topic : Composition, collection and preservation of stool

Book Reference : Talib Pg 70-79

Video : https://www.youtube.com/watch?v=gtnPYZWfnSU

Presentation : https://www.slideshare.net/drsudeesh/stool-examination

Session 24

Topic : Physical and Chemical examination of Stool

Book Reference : Talib Pg 70-79

Video : https://www.youtube.com/watch?v=NEAmF0Qnyik

Presentation : https://www.slideshare.net/drsudeesh/stool-examination

Session 25

Practice session

Topic : Stool Analysis : Collection and physical examination

Video : https://www.youtube.com/watch?v=-iI2PxmHxuo&t

Presentation : https://www.slideshare.net/drsudeesh/stool-examination

Session 26

Topic : Microscopical Examination of Stool

Book Reference : Talib Pg 70-79

Video : https://www.youtube.com/watch?v=_ePqcdDKCe0

Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool

Session 27

Practice session

Topic : Stool Analysis Microscopic Examination : Protozoa

Video : https://www.youtube.com/watch?v=MRzUXg8kFiY

Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool

Session 28

Practice Session

Topic : Stool Analysis Microscopic Examination : Helminthes

Video : https://www.youtube.com/watch?v=H6mCLzpLPz0

Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool

Session 29

Practice session

Topic : Stool Analysis : Chemical Examination

Video : https://www.youtube.com/watch?v=-iI2PxmHxuo&t

Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool

Session 30

Practice session

Topic : Stool Analysis :  Occult test and reducing sugar

Video : https://www.youtube.com/watch?v=Mqus-OtJTYI

Presentation : https://www.slideshare.net/priyankaBuragohain1/routine-examination-of-stool

Session 31

Topic : Difference between Amoebic Dysentery and Bacillary Dysentery

Book Reference : Mukherjee Pg 880-885

Video : https://www.youtube.com/watch?v=DIH5XB5UK5k

Presentation : https://www.slideshare.net/NishaPaudel1/differences-between-amoebic-and-bacillary-dysentery

Session 32

Topic : Clinical significance and Report writing of Stool

Book Reference : Mukherjee Pg 880-885

Video : https://www.youtube.com/watch?v=-iI2PxmHxuo

Presentation : https://www.slideshare.net/odairjoub/stool-analysis-84232092

Session 33

Topic : Sputum for the diagnosis of Mycobacterium tuberculosis

Book Reference : Godkar Pg 1539-1543

Video : https://www.youtube.com/watch?v=wY7ErwZlk48

Presentation : https://www.slideshare.net/AlfredJoel/sputum-examination-and-analysis

Session 34

Practice Session

Topic :Sputum Analysis : Collection and physical examination

Video : https://www.youtube.com/watch?v=FH9eiWp0TZ4

Presentation : https://www.slideshare.net/AlfredJoel/sputum-examination-and-analysis

Session 35

Topic : Basic of DOT Centre, Report writing and report analysis of sputum

Video : https://www.youtube.com/watch?v=dSMQJ_UbeK4

Presentation : https://www.slideshare.net/AlfredJoel/sputum-examination-and-analysis

Session 36

Topic : Respiratory Tract Infection: Gram Staining and ZN Staining

Book Reference : Godkar Pg 1539-1543

Video : https://www.youtube.com/watch?v=L1x6LoL7pTw

Presentation : https://www.slideshare.net/shanthoshpriyan/rntcp-57543326

Session 37

Session Plan

Topic : Sputum Analysis : Tuberculosis(ZN Stain)

Video : https://www.youtube.com/watch?v=wY7ErwZlk48

Presentation : https://www.slideshare.net/shanthoshpriyan/rntcp-57543326

Session 38

Session Plan

Topic : Sputum Analysis : Respiratory infection(Gram Stain)

Video : https://www.youtube.com/watch?v=2MaZyId3XXY

Presentation : https://www.slideshare.net/shanthoshpriyan/rntcp-57543326

Session 39

Topic : Semen : Physical examination, Chemical Examination and Microscopical Examination

Book Reference : Mukherjee 871,879

Video : https://www.youtube.com/watch?v=0AK_ops65LI

Presentation : https://www.slideshare.net/drvipinDrvipinsharma/semen-analysis-91057379

Session 40

Practice Session

Topic : Semen Analysis : Collection and physical examination

Video : https://www.youtube.com/watch?v=0AK_ops65LI&t

Presentation : https://www.slideshare.net/drvipinDrvipinsharma/semen-analysis-91057379

Session 41

Practice Session

Topic :Semen Analysis : Chemical Examination

Video : https://www.youtube.com/watch?v=0AK_ops65LI&t

Presentation : https://www.slideshare.net/drvipinDrvipinsharma/semen-analysis-91057379

Session 42

Practice Session

Topic : Semen Analysis : Microscopical examination

Video : https://www.youtube.com/watch?v=wMcprAUe5Z0

Presentation : https://www.slideshare.net/drvipinDrvipinsharma/semen-analysis-91057379

Session 43

Topic : Report analysis and report writing of Semen

Book Reference : Tahlib Pg 66-67

Video : https://www.youtube.com/watch?v=Unt3EwbM-rA

Presentation : https://www.slideshare.net/danish29/semen-analysis-61887227

Session 44

Topic : CSF: Composition, Collection, Preservation and physical examination

Book Reference : Mukherjee Pg 850-857

Video : https://www.youtube.com/watch?v=LZqiu6PKrZU

Presentation : https://www.slideshare.net/UsmanAkbar4/csf-41592002

Session 45

Practice Sesion

Topic : CSF Analysis : Collection and Routine Examination

Video : https://www.youtube.com/watch?v=KSCFYLUJc7U&t

https://www.youtube.com/watch?v=LZqiu6PKrZU&t

Presentation : https://www.slideshare.net/UsmanAkbar4/csf-41592002

Session 46

Topic : Examination of CSF related to Meningitis, Brain Tumour and other disorder

Book Reference : Mukherjee Pg 865-869

Video : https://www.youtube.com/watch?v=gIHUJs2eTHA

Presentation : https://www.slideshare.net/bahoran03/meninges-and-related-tumors

Session 47

Topic : Synovial fluid : Collection and preservation

Book Reference : Godkar Pg 1544

Video : https://www.youtube.com/watch?v=0_fxPCQYUiA

Presentation : https://labtestsonline.org/tests/synovial-fluid-analysis

Session 50

Topic : Routine laboratory investigation of Pericardial Fluid

Book Reference : Godkar Pg 1547-1548

Video : https://www.youtube.com/watch?v=wKYWhutqzyg

Presentation : https://www.slideshare.net/YESANNA/pericardial-fluid

Session 51

Practice Session 

Topic : Pericardial Fluid : Collection and routine examination

Video : https://www.youtube.com/watch?v=ddahqoVGmIc&t

Presentation : https://www.slideshare.net/YESANNA/pericardial-fluid

Session 52

Topic : Routine laboratory investigation of Pleural Fluid

Video : https://www.youtube.com/watch?v=ddahqoVGmIc

Presentation : https://www.slideshare.net/nasirnazeer5/pleural-fluid-examination

Session 53

Practice Session

Topic : Pleural Fluid : Collection and routine examination

Video : https://www.youtube.com/watch?v=ddahqoVGmIc&t

Presentation : https://www.slideshare.net/nasirnazeer5/pleural-fluid-examination

Session 54

Our Main Teachers

Sunil Kumar Jha is working as the Dean of School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management since 2016. He has also taken the responsibility of Director of Community Diagnostic Center. His major areas of expertise are medical diagnostics , clinical pathology, diagnostic bacteriology, histopathology and patient safety. He […]