Diagnostic Bacteriology
Teacher
Dr. Monali Priyadarsini Mishra
Category
Core Courses
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Course Name : CUTM1747-Diagnostic Bacteriology
Code(Credit) : (3-2-0)
Course Objectives
- To confirm the suspicion of infectious bacterial disease.
- To identify the etiologic agent by isolating the causative bacterial pathogen.
Learning Outcomes
- Study of Lab diagnosis for Enteric infection, Respiratory tract Infection, Oral & Stomach infection, Urinary tract infections.
- Study of control measures for nosocomial infection.
- Student can safeguard himself & society and can work diagnostics and hospitals
Course Syllabus
Module -1
Aerobic Culture: Scope and importance of aerobic culture, Factors affecting aerobic culture, Various media and techniques of aerobic culture. Laboratory Diagnosis of Enteric Infections: (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods) Enteric fever / Typhoid fever, Bacterial endocarditis, Bacteraemia, Septicemia, Pyrexia of unknown origin (PUO).
Practice:
- Isolation & identification of different groups of bacteria in laboratory
- Antibiotic susceptibility test
- Preparation and use of different stains in bacteriology laboratory Grams stain, ZN stain, Albert stain, Spore stain, Capsule stain, Flagella stain, Motility test
Module -2
Laboratory Diagnosis of Respiratory Tract Infection (RTI): (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods) Lower RTI, Upper RTI. Laboratory Diagnosis of Urinary Tract Infection: (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods)
Practice:
- Anaerobic Culture
- Laboratory Diagnosis of Pus
- Laboratory Diagnosis of GI Tract
Module -3
Laboratory Diagnosis of Oral, Throat and Stomach Infection: (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods) Gingivitis and anaerobic infection of oral cavity. Peptic ulcer (with emphasis in mechanism of peptic ulcer caused by Helicobacter pylori), Laboratory Diagnosis of Eye Infection: (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods) Corneal ulcer, Conjunctivitis.
Practice:
- Laboratory Diagnosis of Mycobacterium Infection
- Laboratory Diagnosis of Venereal Diseases
- Performance of different Tests Rapid Diagnostic Tests Molecular Tests 8 hours Interpretation of Test Results 5 hours Test reporting
Suggested References:
- Practical Medical Microbiology by Mackie & MacCartney Volume 1 and2
- Text book of Microbiology by Ananthanereyan
- Medical Microbiology by Paniker & SatishGupte
- Medical Microbiology-by Fritz H. Kayser et al
- Bailey and Scott's Diagnostic Microbiology(12th) Ed
- Medical laboratory Technology vol.I, II, III by Mukherjee
- Medical Laboratory manual for tropical countries Vol II Microbiology by MoniaCheesbrough
Session Plan
Session 1
Aerobic Culture: Scope and importance of aerobic culture
Video: https://www.youtube.com/watch?v=dfmon5w1ZJI
Presentation: https://www.slideshare.net/NimishaK4/cultivation-of-bacteria-ppt-nimisha
Session 2
Various media and techniques of aerobic culture
Video 1: https://www.youtube.com/watch?v=V9IrCh-lFQI
Video 2:https://www.youtube.com/watch?v=KHg_PyjQPwk&t=4s
Presentation: https://www.slideshare.net/doctorrao/culture-media3
Session 3
Factors affecting aerobic culture
Video: https://www.youtube.com/watch?v=kwRA3s3qUvw
Presentation: https://www.slideshare.net/anass1250/13-fet1006-factors-affecting-growth-of-micro-organisms
Session 4
Factors affecting aerobic culture
Video: https://www.youtube.com/watch?v=kwRA3s3qUvw
Presentation: https://www.slideshare.net/anass1250/13-fet1006-factors-affecting-growth-of-micro-organisms
Session 5
Enteric fever / Typhoid fever
Video 1: https://www.youtube.com/watch?v=XkZTS8ep5wQ
Video 2: https://www.youtube.com/watch?v=xOamez79hLA&t=198s
Presentation:https://www.slideshare.net/yuyuricci/enteric-fever-typhoid-fever
Session 6
Enteric fever / Typhoid fever
Video 1: https://www.youtube.com/watch?v=9y3k6ySfZ2Y
Video 2: https://www.youtube.com/watch?v=teobyRfeKBk
Presentation : https://www.slideshare.net/dranwarahmad/typhoid-fever-ppt
Session 7
Bacterial endocarditis
Video: https://www.youtube.com/watch?v=9JUdjT7idXM
Presentation: https://www.slideshare.net/doctorrao/infective-endocarditis-23287027
Session 8
Bacteraemia
Video:https://www.youtube.com/watch?v=HZ4aSoAxaaM
Presentation: https://www.slideshare.net/OmarAnsari14/bacteremia-121256365
Session 9
Septicemia
Video: https://www.youtube.com/watch?v=VWm971N6u1c
Presentation:https://www.slideshare.net/AshishSaxena12/septicemia-11536070
Session 10
Pyrexia of unknown origin (PUO)
Video: https://www.youtube.com/watch?v=NcV8Q5s2AnI
Presentation: https://www.slideshare.net/yuyuricci/pyrexia-of-unknown-origin-puo-93465734
Practice:
Isolation & identification of different groups of bacteria in the laboratory
Practice:
Preparation and use of different stains in bacteriology laboratory Grams stain, ZN stain, Albert stain, Spore stain, Capsule stain, Flagella stain, Motility test
Video 1: https://www.youtube.com/watch?v=c2BPE7wIK20
Video 2:https://www.youtube.com/watch?v=sxa46xKfIOY
Video 3:https://www.youtube.com/watch?v=NmfygqRQdeA
Video 4:https://www.youtube.com/watch?v=0yttbfBsSgk
Video 5:https://www.youtube.com/watch?v=mZGEhql02_I
Session 11
Laboratory Diagnosis of Lower Respiratory Tract Infection (RTI): Terminology, mechanism of infection, etiology
Video: https://www.youtube.com/watch?v=knwJOxuf9B8
Presentation : https://www.slideshare.net/manalihsolanki/lower-respiratory-tract-infections-ppt
Session 12
Laboratory Diagnosis of Lower Respiratory Tract: conventional and rapid diagnostic methods
Video: https://www.youtube.com/watch?v=bBhzufL5Xxk
Presentation: https://www.slideshare.net/doctorrao/lower-respiratory-infections-bacteriological-diagnosis
Session 13
Laboratory Diagnosis of Upper Respiratory Tract Infection (RTI): Terminology, mechanism of infection, etiology
Video:https://www.youtube.com/watch?v=AmmOJ3MtKHg
Presentation: https://www.slideshare.net/E_neutron/upper-respiratory-tract-infection-urti
Session 14
Laboratory Diagnosis of Upper Respiratory Tract Infection (RTI):conventional and rapid diagnostic methods
Video: https://www.youtube.com/watch?v=ONXr_Zt4M1M
Presentation: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200861/
Session 15
Laboratory Diagnosis of Urinary Tract Infection: Terminology, mechanism of infection, etiology
Video: https://www.youtube.com/watch?v=lY2bZjggc08
Presentation: https://www.slideshare.net/bindupulugurtha/urinary-tract-infections-11817974
Session 16
Laboratory Diagnosis of Urinary Tract Infection: conventional and rapid diagnostic methods
Video: https://www.youtube.com/watch?v=0FT3Xzn0Q6A
Presentation: https://www.slideshare.net/bausherwillayat/urinary-tract-infection-89635298
Session 17
Laboratory Diagnosis of Urinary Tract Infection: conventional and rapid diagnostic methods
Video:https://www.youtube.com/watch?v=1vIHTAnBmuU
https://www.youtube.com/watch?v=NvgbPEpkU-8
Presentation:https://www.slideshare.net/umang31/urinary-tract-infectionslab-diagnosis-ecoli-klebsilla-proteus-pseudomonas
Session 18
Laboratory Diagnosis of Oral, Throat and Stomach Infection: Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods
Video : https://www.youtube.com/watch?v=uiv7yaBc69I
https://www.youtube.com/watch?v=UP1puhpUyV4&t=79s
https://www.youtube.com/watch?v=IOUnXeqNyMs
Presentation: https://www.slideshare.net/ajaysharma616/sore-throat-59483864
https://www.slideshare.net/krishnameera999/stomach-disorders
Session 19
Gingivitis and anaerobic infection of oral cavity
Video: https://www.youtube.com/watch?v=B35jRf4EKPA
https://www.youtube.com/watch?v=FViqyY8h4wE
https://www.youtube.com/watch?v=X1rF7Iv5Q_k
Presentation: https://www.slideshare.net/AhmedJawad8/bacterial-infections-of-oral-cavity
Session 20
Gingivitis and anaerobic infection of oral cavity
Video: https://www.youtube.com/watch?v=B35jRf4EKPA
https://www.youtube.com/watch?v=FViqyY8h4wE
https://www.youtube.com/watch?v=X1rF7Iv5Q_k
Presentation: https://www.slideshare.net/AhmedJawad8/bacterial-infections-of-oral-cavity
Session 21
anaerobic infection of oral cavity
Video: https://www.youtube.com/watch?v=2zhoyDKS70Q
Presentation: https://www.slideshare.net/shrutisingh98/bacterial-infections-and-their-oral-manifestations
Session 22
Peptic ulcer (with emphasis in mechanism of peptic ulcer caused by Helicobacter pylori)
Video:https://www.youtube.com/watch?v=x3aZDY9Q_Qk
https://www.youtube.com/watch?v=y-shOXdsJeA
Presentation: https://www.slideshare.net/anieduugo/peptic-ulcer-57689431
Session 23
Peptic ulcer (with emphasis in mechanism of peptic ulcer caused by Helicobacter pylori)
Video:https://www.youtube.com/watch?v=x3aZDY9Q_Qk
https://www.youtube.com/watch?v=y-shOXdsJeA
Presentation:https://www.slideshare.net/shaffar75/helicobacter-pylori-infection
Session 24
Laboratory Diagnosis of Eye Infection: Terminology, mechanism of infection, etiology
Video: https://www.youtube.com/watch?v=ydKbESsC3es
Video: https://www.youtube.com/watch?v=TuOCt6Zatuc
Presentation: https://www.slideshare.net/hongchiong/4-copy-of-eye-infections-new
Session 25
Laboratory Diagnosis of Eye Infection: Terminology, mechanism of infection, etiology
Video: https://www.youtube.com/watch?v=ydKbESsC3es
Video: https://www.youtube.com/watch?v=TuOCt6Zatuc
Presentation: https://www.slideshare.net/hongchiong/4-copy-of-eye-infections-new
Session 26
Corneal ulcer
Video: https://www.youtube.com/watch?v=u_wxRh1Rz2o
Video: https://www.youtube.com/watch?v=qTrGopWa3pw
Presentation : https://www.slideshare.net/sameep94/corneal-ulcers
Session 27
Conjunctivitis
Video: https://www.youtube.com/watch?v=gQOMO4wjodM
Presentation: https://www.slideshare.net/hmirzaeee/conjunctivitis-11363861
Practice:
Laboratory Diagnosis of Mycobacterium Infection
Practice:
Laboratory Diagnosis of Venereal Diseases
Practice:
Performance of different Tests Rapid Diagnostic Tests Molecular Tests 8 hours Interpretation of Test Results 5 hours Test reporting
Case Studies
Case Studies
Our Main Teachers
Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]