Diagnostic Bacteriology

Teacher

Dr. Monali Priyadarsini Mishra

Category

Core Courses

Course Attendees

Still no participant

Course Reviews

Still no reviews

Course Name : Diagnostic Bacteriology

Code(Credit) : (3-2-0)

Course Objectives

  • To confirm the suspicion of infectious bacterial disease.
  • To identify the etiologic agent by isolating the causative bacterial pathogen.

Learning Outcomes

  • Study of Lab diagnosis for Enteric infection, Respiratory tract Infection, Oral & Stomach infection, Urinary tract infections.
  • Study of control measures for nosocomial infection.
  • Student can safeguard himself & society and can work diagnostics and hospitals 

Course Syllabus

Module -1

Aerobic Culture: Scope and importance of aerobic culture, Factors affecting aerobic culture, Various media and techniques of aerobic culture. Laboratory Diagnosis of Enteric Infections: (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods) Enteric fever / Typhoid fever, Bacterial endocarditis, Bacteraemia, Septicemia, Pyrexia of unknown origin (PUO).

Practice:

  • Isolation & identification of different groups of bacteria in laboratory
  • Antibiotic susceptibility test
  • Preparation and use of different stains in bacteriology laboratory Grams stain, ZN stain, Albert stain, Spore stain, Capsule stain, Flagella stain, Motility test

Module -2 

Laboratory Diagnosis of Respiratory Tract Infection (RTI): (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods) Lower RTI, Upper RTI. Laboratory Diagnosis of Urinary Tract Infection: (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods)

Practice:

  • Anaerobic Culture
  • Laboratory Diagnosis of Pus
  • Laboratory Diagnosis of GI Tract

Module -3 

Laboratory Diagnosis of Oral, Throat and Stomach Infection: (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods) Gingivitis and anaerobic infection of oral cavity. Peptic ulcer (with emphasis in mechanism of peptic ulcer caused by Helicobacter pylori), Laboratory Diagnosis of Eye Infection: (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods) Corneal ulcer, Conjunctivitis.

Practice:

  • Laboratory Diagnosis of Mycobacterium Infection
  • Laboratory Diagnosis of Venereal Diseases
  • Performance of different Tests Rapid Diagnostic Tests Molecular Tests 8 hours Interpretation of Test Results 5 hours Test reporting

Suggested Readings:

  • Practical Medical Microbiology by Mackie & MacCartney Volume 1 and2
  • Text book of Microbiology by Ananthanereyan
  • Medical Microbiology by Paniker & SatishGupte
  • Medical Microbiology-by Fritz H. Kayser et al
  • Bailey and Scott's Diagnostic Microbiology(12th) Ed
  • Medical laboratory Technology vol.I, II, III by Mukherjee
  • Medical Laboratory manual for tropical countries Vol II Microbiology by MoniaCheesbrough

Session Plan

Session 1

Aerobic Culture: Scope and importance of aerobic culture

Video: https://www.youtube.com/watch?v=dfmon5w1ZJI

Presentation: https://www.slideshare.net/NimishaK4/cultivation-of-bacteria-ppt-nimisha

Session 2

Session 3

Session 4

Session 5

Session 6

Session 7

Session 8

Session 9

Session 10

Practice:

Isolation & identification of different groups of bacteria in the laboratory

Video: https://www.youtube.com/watch?v=LKbS7YisunM

Practice:

Antibiotic susceptibility test

Video: https://www.youtube.com/watch?v=Np87w5kCL-4&t=20s

Practice:

Preparation and use of different stains in bacteriology laboratory Grams stain, ZN stain, Albert stain, Spore stain, Capsule stain, Flagella stain, Motility test

Video 1: https://www.youtube.com/watch?v=c2BPE7wIK20

Video 2:https://www.youtube.com/watch?v=sxa46xKfIOY

Video 3:https://www.youtube.com/watch?v=NmfygqRQdeA

Video 4:https://www.youtube.com/watch?v=0yttbfBsSgk

Video 5:https://www.youtube.com/watch?v=mZGEhql02_I

Video 6: https://www.youtube.com/watch?v=cXYfT5hSLoQ

video 7: https://www.youtube.com/watch?v=jTTKMVxJSy4

Session 11

Laboratory Diagnosis of Lower Respiratory Tract Infection (RTI): Terminology, mechanism of infection, etiology

Video: https://www.youtube.com/watch?v=knwJOxuf9B8

Presentation : https://www.slideshare.net/manalihsolanki/lower-respiratory-tract-infections-ppt

Session 12

Laboratory Diagnosis of Lower Respiratory Tract: conventional and rapid diagnostic methods

Video: https://www.youtube.com/watch?v=bBhzufL5Xxk

Presentation: https://www.slideshare.net/doctorrao/lower-respiratory-infections-bacteriological-diagnosis

Session 13

Laboratory Diagnosis of Upper Respiratory Tract Infection (RTI): Terminology, mechanism of infection, etiology

Video:https://www.youtube.com/watch?v=AmmOJ3MtKHg

Presentation: https://www.slideshare.net/E_neutron/upper-respiratory-tract-infection-urti

Session 14

Laboratory Diagnosis of Upper Respiratory Tract Infection (RTI):conventional and rapid diagnostic methods

Video: https://www.youtube.com/watch?v=ONXr_Zt4M1M

Presentation: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6200861/

Session 15

Laboratory Diagnosis of Urinary Tract Infection: Terminology, mechanism of infection, etiology

Video: https://www.youtube.com/watch?v=lY2bZjggc08

Presentation: https://www.slideshare.net/bindupulugurtha/urinary-tract-infections-11817974

Session 16

Laboratory Diagnosis of Urinary Tract Infection: conventional and rapid diagnostic methods

Video: https://www.youtube.com/watch?v=0FT3Xzn0Q6A

Presentation: https://www.slideshare.net/bausherwillayat/urinary-tract-infection-89635298

Session 17

Practice:

Anaerobic Culture

Video: https://www.youtube.com/watch?v=aFDYx-7ceS8

Practice:

Laboratory Diagnosis of Pus

Video: https://www.youtube.com/watch?v=dXeCRWTn7n4

Practice:

Laboratory Diagnosis of GI Tract

Video: https://www.youtube.com/watch?v=T2lXG5MKVA8

Session 18

Laboratory Diagnosis of Oral, Throat  and Stomach Infection: Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods

Video : https://www.youtube.com/watch?v=uiv7yaBc69I

https://www.youtube.com/watch?v=UP1puhpUyV4&t=79s

https://www.youtube.com/watch?v=IOUnXeqNyMs

Presentation: https://www.slideshare.net/ajaysharma616/sore-throat-59483864

https://www.slideshare.net/krishnameera999/stomach-disorders

Session 19

Session 20

Session 21

Session 22

Peptic ulcer (with emphasis in mechanism of peptic ulcer caused by Helicobacter pylori)

Video:https://www.youtube.com/watch?v=x3aZDY9Q_Qk

https://www.youtube.com/watch?v=y-shOXdsJeA

Presentation: https://www.slideshare.net/anieduugo/peptic-ulcer-57689431

Session 23

Peptic ulcer (with emphasis in mechanism of peptic ulcer caused by Helicobacter pylori)

Video:https://www.youtube.com/watch?v=x3aZDY9Q_Qk

https://www.youtube.com/watch?v=y-shOXdsJeA

Presentation:https://www.slideshare.net/shaffar75/helicobacter-pylori-infection

Session 24

Laboratory Diagnosis of Eye Infection: Terminology, mechanism of infection, etiology

Video: https://www.youtube.com/watch?v=ydKbESsC3es

Video: https://www.youtube.com/watch?v=TuOCt6Zatuc

Presentation: https://www.slideshare.net/hongchiong/4-copy-of-eye-infections-new

Session 25

Laboratory Diagnosis of Eye Infection: Terminology, mechanism of infection, etiology

Video: https://www.youtube.com/watch?v=ydKbESsC3es

Video: https://www.youtube.com/watch?v=TuOCt6Zatuc

Presentation: https://www.slideshare.net/hongchiong/4-copy-of-eye-infections-new

Session 26

Session 27

Practice:

Laboratory Diagnosis of Mycobacterium Infection

Video: https://www.youtube.com/watch?v=RwifN15FrOc

Practice:

Laboratory Diagnosis of Venereal Diseases

Video: https://www.youtube.com/watch?v=RlwykBWQuDA

Practice:

Performance of different Tests Rapid Diagnostic Tests Molecular Tests 8 hours Interpretation of Test Results 5 hours Test reporting

Video: https://www.youtube.com/watch?v=RlwykBWQuDA

Case Studies

Case Studies

Recent Comments

    Our Main Teachers

    Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]