Diagnostic Parasitology

Teacher

Miss Sonali Dash

Category

Core Courses

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Course Name : Diagnostic Parasitology

Code(Credit) : CUTM1744(3-1-0)

Course Objectives

  • Recognize the disgnostic stage of the infection under the microscope and to manage the infected patient.
  • To examine parasites and parasitism, emphasizing the influence of parasites on the ecology and evolution of free-living species, and the role of parasites in global public health.

Learning Outcomes

  • Organise sample collection, transportation, processing and storage in an appropriate manner.
  • Plan, write and implement research projects in parasitology, analyze their results and publish these in peer-reviewed journals.
  • Coordinate with concerned agencies regarding protozoan and helminth diseases and their outbreaks.
  • Plan and execute epidemiological studies and provide advice in relation to protozoan diseases.

Course Syllabus

Module I

Laboratory Organization (Parasitology Lab). Selection, Collection, Perseveration and Transportation of Samples.

Practice 

Safety measures in parasitology lab and laboratory organization.

Module II

Laboratory Diagnosis, of the Intestinal and Vaginal Parasites: (Terminology, mechanism of infection, etiology, conventional and rapid diagnostic methods)

Protozoa: Entamoeba histolytica, Giardia lamblia, Trichomonas spp, Cyrptosporidium, Cyclospora cayantenensis, Isospora.

Helminthes: Ascaries lumbricoides, Hook worm: Anacyclostoma and Necator, Enterobius vermicularis, Trichuris trichuira, Strongloides spp., Taenia spp., Echinococcus spp., Hymonolepis nana. Tissue and Blood Parasites:Malaria spp, Leishmania spp(Kalaazar), Wachereria spp. Brugia, Loaloa, Oncocerca, Dracuhculus, Paragonimus westermani/hertmani.

Practice

Examination of stool and identification of parasites. Observation of sample for parasite identification.

Module III

Laboratory Diagnosis of Various Parasites: Direct method, Indirect method, Rapid methods, Molecular Technique -Parasite Culture. Different Stains used in Diagnostic Parasitology.

Practice

Identification of different parasites and  their morphology from slide’s study.

Suggested Readings

Parasitology book by K.D. Chatterjee 13th Ed. Protozoology and Helminthology.

Textbook of medical Parasitology.

Ebook Link- https://www.pdfdrive.com/textbook-of-medical-parasitology-textbook-of-medical-parasitology-e128716897.html

Online Tutorial Links

Stool Examination.

ELink- https://www.youtube.com/watch?v=-iI2PxmHxuo

https://www.youtube.com/watch?v=MRzUXg8kFiY

https://www.youtube.com/watch?v=_ePqcdDKCe0

Malaria thick smear preparation.

ELink- https://www.youtube.com/watch?v=WPP7AjmStBg

Malaria thin smear preparation.

ELink- https://www.youtube.com/watch?v=acoALifVvb8

 

 

Session Plan

Session 1

Lecture Topic- Laboratory Organization (Parasitology Lab)

Lecture Video- https://www.youtube.com/watch?v=U8mLe8xh5yM

Lecture Notes-http://whqlibdoc.who.int/publications/9241544104_(part1).pdf?ua=1

Session 2

Lecture Topic-Selection, Collection, Perseveration and Transportation of Samples.

Lecture Video- https://www.youtube.com/watch?v=heTQcEMDSGU

Lecture Notes- http://whqlibdoc.who.int/publications/9241544104_(part1).pdf?ua=1

Session 3

Practice Topic- Safety measures in parasitology lab and laboratory organization.

Practice Video- https://www.youtube.com/watch?v=U8mLe8xh5yM

Session 4

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Entamoeba histolytica.

Lecture Video- https://www.youtube.com/watch?v=Klx0uDfCIRA

Lecture Presentation-https://www.slideshare.net/RaNaMB1/entamoeba-histolytica-95813165

Lecture Readings- Parasitology by K D Chatterjee(13th Ed), pg.18-41-chapter II.

Session 5

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Giardia lamblia.

Lecture Video- https://www.youtube.com/watch?v=cpu9PRIEYWY

Lecture Presentation- https://www.slideshare.net/azizkhan1995/giardia-lamblia-63763763

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.47-48-chapter II

Session 6

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Trichomonas spp.

Lecture Video- https://www.youtube.com/watch?v=5t6sHgRqavw

Lecture Presentation- https://www.slideshare.net/PakRose1/trichomonas-31612943

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.49-51-chapter II

Session 7

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Cyrptosporidium.

Lecture Video- https://www.youtube.com/watch?v=RIFVlIrd8L0

Lecture Presentation- https://www.slideshare.net/alisaqlain111/cryptosporidium-39089694

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.134-chapter III

Session 8

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Cyclospora.

Lecture Video- https://www.youtube.com/watch?v=dVFRgmZpTfQ

Lecture Notes-  https://www.cdc.gov/parasites/cyclosporiasis/index.html

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.135-chapter III

Session 9

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Isospora.

Lecture Video- https://www.youtube.com/watch?v=CyMvK1dumb8

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.128-chapter III

Session 10

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Ascaries lumbricoides.

Lecture Video- https://www.youtube.com/watch?v=4U3bIXaosh0

https://youtu.be/UIXZYF1dZQ4

Lecture Presentation- https://www.slideshare.net/sakuramarnati/ascaris-lumbricoides-14172492

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.229-232-chapter VIII

Session 11

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Hook worm: Anacyclostoma.

Lecture Video- https://www.youtube.com/watch?v=QIWc-55IQzk

https://www.youtube.com/watch?v=HXAiDBiZ7FA

Lecture Presentation- https://www.slideshare.net/RaNaMB1/ancylostoma-duodenale-hookwormi2015

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.212-220-chapter VII

Session 12

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Enterobius vermicularis.

Lecture Presentation- https://www.slideshare.net/drmoiz/enterobius-vermicularispinworm

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.225-chapter VII

Session 13

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Trichuris trichuira.

Lecture Video- https://www.youtube.com/watch?v=ZDIHQ_FG_68

Lecture Presentation- https://www.slideshare.net/anady28/trichuris-trichiura-11658065

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.205-206-chapter VII

Session 14

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Strongloides spp.

Lecture Video- https://www.youtube.com/watch?v=5MuXsdIZSEc

Lecture Presentation- https://www.slideshare.net/MizanRahman9/strongyloides-stercoralis-63214733

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.208-212chapter VII

Session 15

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Taenia spp.

Lecture Video- https://www.youtube.com/watch?v=kNEYwXi-5_4

Lecture Presentation- https://www.slideshare.net/doctorrao/tinea-solium

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.151-chapter VI

Session 16

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Echinococcus spp.

Lecture Video- https://www.youtube.com/watch?v=_rdb5j7jDhQ

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.165-chapter VI

Session 17

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Malaria (Plasmodium) spp.

Lecture Video- https://www.youtube.com/watch?v=1v55yg0RfoY

Lecture Presentation- https://www.slideshare.net/AbhishekJoshi151/malaria-72552248

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.90-102-chapter III

Session 18

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Leishmania spp.

Lecture Video- https://www.youtube.com/watch?v=wzjSmxThgJ0

Lecture Notes- https://www.who.int/news-room/fact-sheets/detail/leishmaniasis

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.64-68chapter II

Session 19

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Wuchereria spp.

Lecture Video- https://www.youtube.com/watch?v=9zMdg9PFCW0

Lecture Presentation- https://www.slideshare.net/alubajessabeth/wuchereria-bancrofti-39245224

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.238-247chapter VIII

Session 20

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Oncocerca.

Lecture Presentation- https://www.slideshare.net/luvmatyns/onchocerciasis

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.249-251-chapter VIII

Session 21

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Dracuhculus.

Lecture Presentation- https://www.slideshare.net/don.clark/dracunculiasis-dracunculus-medinensis-guinea-worm-disease

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.254-258chapter VII

Session 22

Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Paragonimus westermani.

Lecture Video- https://www.youtube.com/watch?v=en1bKReS0bA

Lecture Presentation- https://www.slideshare.net/amin7ma/paragonimiasis-lung-fluke-disease

Lecture Readings-Parasitology by K D Chatterjee(13th Ed), pg.196-198 chapter VII

Session 23

Session 24

Lecture Topic- Laboratory diagnosis method (Direct method).

Lecture Video- https://www.youtube.com/watch?v=r69QmZQc0Qc

https://www.youtube.com/watch?v=-iI2PxmHxuo

Lecture Presentation- https://www.slideshare.net/Prbn/lab-dia-of-parasite

Session 25

Lecture Topic- Laboratory diagnosis method (Indirect Method)

Lecture Video- https://www.youtube.com/watch?v=WTelVO4ywUc

Lecture Presentation- https://www.slideshare.net/Prbn/lab-dia-of-parasite

Session 26

Lecture Topic- Laboratory diagnosis method (Rapid Methods)

Lecture Video- https://www.youtube.com/watch?v=hft79S__C_8

Lecture Notes- https://www.oie.int/doc/ged/D8270.PDF

Session 27

Lecture Topic- Laboratory diagnosis method (Molecular Technique)

Lecture Video- https://www.youtube.com/watch?v=nbYGjO5fDA8

Lecture Notes- https://www.hindawi.com/journals/ipid/2009/278246/

Session 29

Lecture Topic- Parasite Culture methods.

Lecture Video- https://www.youtube.com/watch?v=1LdYQ5Utu3Y

Lecture Presentation- https://www.slideshare.net/prdiphamal/parasite-culture

Session 30

Session 31

Practice Topic- Identification of parasite and morphology study by slides study. ( Malaria Thick smear preparation)

Practice Video- https://www.youtube.com/watch?v=WPP7AjmStBg

Session 32

Practice Topic- Malaria Thin smear Preparation.

Practice Video- https://www.youtube.com/watch?v=acoALifVvb8

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