Track Total Credits ( T-P-P): 9-9-11

Courses Division (list all divisions):

Track courses (3-6-0)

1. Stem Cell and Developmental Biology (1+2+0)
2. Animal Cell Culture and its Application (1+2+0)
3. Instrumentation of Animal Biotechnology (1+2+0)

Project Courses (0-3-6)

1. Handling of Mammalian Cell Culture (0+1+2)
2. Effect of different phytochemicals on Cell Culture (0+1+2)
3. Handling of Instruments and maintenance  of culture room (0+1+2)

AELP Linked Project Courses (0-0-11) (For Agriculture Students only)

1. To develop the In-Silico analysis for developing drugs
2. To develop a full In-vitro analysis for analysis of drugs
3. Development and Application of pesticides in animal feed and food products
4. Development and Application of drug designing in lab
5. Products/Patents/Publications

Course Objectives:

1. To study the advantages of natural medicines over conventional Medicine to avoid potential side-effects.
2. To Study about stem cell and its application in preventing cancer
3. To learn about developmental biology to study the effect of delivering a drug to animal.
4. To learn about Drug Designing, and detailed Protein structure, pharmacophore, basics of Docking and Discovery studio and application in Agriculture, Fisheries and other Science

Domain Syllabus:

Subject 1:

Stem Cell and Developmental Biology: (1+2)

Theory:

Introduction: Cellular potency, lineage commitment, cellular development, differentiation, dedifferentiation & trans differentiation, Cell cycle control, Immortal DNA strand hypothesis, Asymmetric cell division, telomerases in relevance to stem cell development and differentiation.

Somatic and Germ cell derived stem cells: Germ line stem cells and germline, derived pluripotent cells, Stem cell niche, epithelial stem cells, mesenchymal stem cells, neural stem cells, haematopoitic stem cells, cardiac stem cells, Cancer stem cells, Markers, molecular and evolutionary mechanisms addressing origin and maintenance of cancer stem cells.

Regulatory mechanisms in Embryonic and adult stem cells: Coreregulatory circuitry, DNAmethylation, histone modifications, histone modifiers, chromatin remodelers, RNA Pol II code, post transcriptional control of gene expression in ESC: role of miRNAs, LincRNAs and RNA binding proteins. Spatial organization of genome during ESC development and differentiation.

Basic concepts of development: Potency, commitment, specification (autonomous, regulative and syncytial), induction, competence, determination and differentiation, morphogenetic gradients, cell fate and cell lineages, genomic equivalence and the cytoplasmic determinants, imprinting.

Gametogenesis and Fertilization: Production and structure of gametes, cell surface molecules in sperm egg recognition in animals, acrosome reaction, fast and slow block to polyspermy, zygote formation.

Practical:

1. Media Preparation and filtration
2. Different buffer preparation
3. Culturing and sub culturing
4. cell viability assay

Subject 2:

Animal Cell Culture and its Application: (1+2)

Media and Reagents: Types of cell culture media for plants, animals and microbial cells; Ingredients of media; Physiochemical properties; Buffers; Oxygen; Osmolarity; Temperature; Balance salt solutions; Antibiotics, growth supplements; Conditioned media; Other cell culture reagents; Preparation and sterilization of cell culture media and other reagents.

Animal Cell Cultures Techniques: History of animal cell culture; Different tissue culture techniques; Types of primary culture; Chicken embryo fibroblast culture; Chicken liver and kidney culture; Secondary culture; Trypsinization; Cell separation; Continuous cell lines; Suspension culture; Organ culture; Behaviour of cells in culture conditions: division, growth pattern, metabolism of estimation of cell number; Development, Characterization and maintenance of cell lines, Cryopreservation; Commercial scale production of animal cells, stem cells and their application; Application of animal cell culture for in vitro testing of drugs; Testing of toxicity of environmental pollutants in cell culture.

Stem cell and progenitor cell assays: Purification of tissue specific stem cells and transplantations. Hematopoietic progenitor cell analyses such as flow cytometry, dynabeads, colony forming assays and in&vitro differentiation assays of lymphoid (BandT), myeloid lineages and other tissue lineages.

Brief overview:  Development in Health and Disease including birth defects, Development and the Environment (biotic, abiotic, and symbiotic regulation), Development and Evolution (developmental mechanisms of evolutionary change). Cell cycle analysis and Synchronization of cultures, cancer studies using cell culture, production of hybridoma and monoclonal antibody production. Animal cloning,  Therapeutic cloning, Tissue engineering, Knock out animals.

Practical:

1. Basic Cell Culture methods
2. Primary cell culture

Subject 3:

Instrumentation of Animal Biotechnology: (1+2)

Equipment and Materials for animal Cell Culture Technology

Basic Aseptic Techniques, Design of Tissue Culture Laboratory , Equipment : Laminar Flow Hoods, CO2 incubator, Open and closed cultures, ELISA plate Reader, Microscopes, centrifuge, Refrigerators and  Freezers, handling of pipetting aids, Miscellaneous small items of  Equipment, Materials, filters, Miscellaneous Items.

Biology and Characterization of cultured cells: Contamination Testing of Culture, Viability measurement and cytotoxicity, Measurement of growth parameters, Cell cycle analysis and Synchronization of cultures, Evaluation of Chemical carcinogenicity, Cell malignancy Testing,  Toxicity Testing, Karyotyping and cytogenetic characterization

Practical:

1. Autoclaving of instruments
2. Maintenance of animal cell culture room
3. Karyotyping
4. Toxicity testing

Projects:

1. Effects of indigenous medicinal plants on different cancer cell lines
2. Purification of Phytochemicals and its application
3. Chemical analysis of different phytochemicals and applications in cell lines
4. Drug delivery in cell lines

Domain Book:

Stem Cell and Developmental Biology:

1. "Stem Cells: From Biology to Therapy 2 Volume Set (Current Topics from the Encyclopedia of Molecular Cell Biology and Molecular Medicine)" by Robert A Meyers
2. Principles of Stem Cell Biology and Cancer: Future Applications and Therapeutics,Editor(s):Tarik Regad, Thomas J. Sayers, Robert C. Rees