Fish Immunology
Teacher
Dr. Ngairangbam Sushila
Category
Core Courses
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Course Name : Fish Immunology
Code(Credit) : FSHM 2106 (1-1-0)
Course Objectives
- To learn the basic principles of Immunology, types of immunity and antigen-antibody interactions.
- To understand the defense mechanism in finfish and shellfish and learn the Serological methods in disease diagnosis.
Course Outcomes
CO-1: Understand the defence mechanism in finfish and shellfish:PO1, PO2
CO-2: Knowledge of the Role of stress and host defence mechanism in disease development: PO1, PO2
CO-3: Understand Serological Methods in disease diagnosis: : PO1, PO2
PO1: Knowledge enhancement: Enhanced knowledge on concepts, essential facts, basics, principles and theories relating to fishery biology, biodiversity and aquatic environment, aquaculture, fish nutrition, fish physiology, genetics and biotechnology, fish processing, fisheries engineering, fish microbiology, fishery extension, economics, and biostatistics.
PO2: Professional and Entrepreneurial Skill development: Understanding the value and processes of life-long learning and professional enhancement and entrepreneurship development.
Course Syllabus
Theory
Introduction, brief history to immunology. Types of immunity: Innate and adaptive immunity, cell mediated and humoral immunity, cells and organs of the immune system. Antigens – structure and types. epitopes, haptenes.Antibody – fine structure, classes with structure and functions, antigenic determinants on immunoglobulins.MHC complex – types, structure, and functions.Antigen-antibody interactions- principle, antigenrecognition by B-cells and T cells.Antigen-antibody reaction - Precipittin reactions, agglutination reactions, Microorganisms associated with fishes in health and disease.Defense mechanism in finfish and shellfish- specific and non specific immune system.Pathogenicity and virulence.Sources of infection, transmission of disease producing organisms, portals of infection.Immunity to bacteria, fungi and parasites Role of stress and host defense mechanism in disease development.Vaccines - types of vaccines – whole cell vaccine, purified macromolecules, recombinant –vector, DNA vaccines and multivalent subunit vaccines, modes of vaccine administration.Serological methods in disease diagnosis.Immunostimulants –types, mechanism of action, modes of administration. Immunoassays, immunodiffusion, ELISA, immunofluorescence, neutralization, radioimmunoassay, serotyping
Practical
1. Collection, separation and identification of fish leucocytes.
2. Separation of blood plasma and serum.
3. Differential counting - RBC and WBC by Haemocytometer.
4. Study of different types of leukocytes and isolation of macrophages.
5. Precipitin reactions - Agglutination test,immunogel diffusion, double immuno diffusion, radial immuno diffusion assay, ELISA.
6. Methods of vaccine preparation and techniques of fish immunization.
Reference book:
- Fish and Shell fish immunology ---- P.Swain, P.K.Sahoo, S.Ayappan
- Prevention and control of fish and prawn diseases ----KP Biswas
Session Plan
Theory
Session 1
Session1: Introduction to immunology
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-I.pdf
YouTube Link https://www.youtube.com/watch?v=wHCJUMBKgyo
Session 3: Adaptive Immunity
Session 4: Cell mediated and humoral immunity
PDF:CMI and humoral
Session 5: Cells and organs of immune system
PDF: Cells and organs
Session 8: Antigenic determinants on immunoglobulins
Session 10: Antigen-antibody interactions
Session 11: Antigen-antibody reaction - Precipitin reactions, agglutination reactions
PDF: Precipitin reaction
Session 12: Specific and Non specific defence mechanism in Fish
PDF 1. http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-V.pdf
PDF 2. http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-III.pdf
Session 13: Stress and immune response
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XI.pdf
YouTube Link https://www.youtube.com/watch?v=2y54zacDe_8
Session 14: Vaccine
PDF 1: http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XIV.pdf
PDF 2: http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XV.pdf
Video 1: https://www.youtube.com/watch?v=YlKLpQEWILg&t=57s
Video : https://www.youtube.com/watch?v=ccT8B37nYRI
Session 15: Serological methods
PDF:Serological test
Session 16: Immunostimulants
PDF: http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XIII-Immuno-stimulants.pdf
Practicals
Session 1: Collection, separation and identification of fish leucocytes
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/1.-Isolation-of-fish-leucocytes.pdf
YouTube Link https://www.youtube.com/watch?v=NMVFvY3znBA
Session 2: Lymphocyte separation from fish blood
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/2.-lEUCOCYTE-iSOLATION.pdf
YouTube Link https://www.youtube.com/watch?v=LkqNUHz39F8
Session 3: Differential counting - RBC and WBC by Haemocytometer (Neubauer Chamber)
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/3.Cell-count-RBCWBC.pdf
YouTube Link https://www.youtube.com/watch?v=MKS0KM3lr90
Session 4: Separation of Blood Plasma and Serum
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/4.-separation-of-plasma-and-serum.pdf
YouTube Link https://www.youtube.com/watch?v=XAhBzUosvsU&t=154s
Session 5: Latex Agglutination Test
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/5.-Latex-Agglutination-test.pdf
YouTube Link https://www.youtube.com/watch?v=3W67OH3v2lU&t=231s
Session 6: Ouchterlony Double Immunodiffusion Test
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/6.-Double-Immunodiffusion-Test.pdf
YouTube Link https://www.youtube.com/watch?v=4vgyrT0U7S8
Session 7: Antigen Capture Elisa Test
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/7.-Antigen-Capture-ELISA-Test.pdf
YouTube Link https://www.youtube.com/watch?v=YJ0-qQslqqQ&t=1s
Session 8: Methods of Vaccine Preparation and Techniques of Fish Immunization
YouTube Link https://www.youtube.com/watch?v=W-Zi5TitJzk
E-learning: Fish Immunology
Link 1: http://ecoursesonline.iasri.res.in/course/view.php?id=255
Link 2: FISH IMMUNOLOGY
Link 3:http://www1.mans.edu.eg/FacMed/dept/microbiology/pdf/4-Basic-Immunology.pdf
Immunology-MCQ
Assignment
Our Main Teachers
Dr. Ngairangbam Sushila joined the University as an Assistant Professor in the department of Aquatic Animal Health Management, School of Fisheries. She completed her doctoral programme from ICAR-Central Institute of Fisheries Education, Mumbai.
Teaching, Research and Product Development, Business Development.