Fish Immunology

Teacher

Dr. Ngairangbam Sushila

Category

Core Courses

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Course Name : Fish Immunology

Code(Credit) : FSHM 2106 (1-1-0)

Course Objectives

  • To learn the basic principles of Immunology, types of immunity and antigen-antibody interactions.
  • To understand the defense mechanism in finfish and shellfish and learn the Serological methods in disease diagnosis.

Course Outcomes

CO-1: Understand the defence mechanism in finfish and shellfish:PO1, PO2

CO-2: Knowledge of the Role of stress and host defence mechanism in disease development: PO1, PO2

CO-3: Understand Serological Methods in disease diagnosis: : PO1, PO2

PO1: Knowledge enhancement: Enhanced knowledge on concepts, essential facts, basics, principles and theories relating to fishery biology, biodiversity and aquatic environment, aquaculture, fish nutrition, fish physiology, genetics and biotechnology, fish processing, fisheries engineering, fish microbiology, fishery extension, economics, and biostatistics.

PO2: Professional and Entrepreneurial Skill development: Understanding the value and processes of life-long learning and professional enhancement and entrepreneurship development. 

Course Syllabus

Theory

Introduction, brief history to immunology. Types of immunity: Innate and adaptive immunity, cell mediated and humoral immunity, cells and organs of the immune system. Antigens – structure and types. epitopes, haptenes.Antibody – fine structure, classes with structure and functions, antigenic determinants on immunoglobulins.MHC complex – types, structure, and functions.Antigen-antibody interactions- principle, antigenrecognition by B-cells and T cells.Antigen-antibody reaction - Precipittin reactions, agglutination reactions, Microorganisms associated with fishes in health and disease.Defense mechanism in finfish and shellfish- specific and non specific immune system.Pathogenicity and virulence.Sources of infection, transmission of disease producing organisms, portals of infection.Immunity to bacteria, fungi and parasites Role of stress and host defense mechanism in disease development.Vaccines - types of vaccines – whole cell vaccine, purified macromolecules, recombinant –vector, DNA vaccines and multivalent subunit vaccines, modes of vaccine administration.Serological methods in disease diagnosis.Immunostimulants –types, mechanism of action, modes of administration. Immunoassays, immunodiffusion, ELISA, immunofluorescence, neutralization, radioimmunoassay, serotyping

Practical

1. Collection, separation and identification of fish leucocytes.

2. Separation of blood plasma and serum.

3. Differential counting - RBC and WBC by Haemocytometer.

4. Study of different types of leukocytes and isolation of macrophages.

5. Precipitin reactions - Agglutination test,immunogel diffusion, double immuno diffusion, radial immuno diffusion assay, ELISA.

6. Methods of vaccine preparation and techniques of fish immunization.

Reference book:

  1. Fish and Shell fish immunology ---- P.Swain, P.K.Sahoo, S.Ayappan
  2. Prevention and control of fish and prawn diseases ----KP Biswas

Session Plan

Theory

Session 1

Session 2: Innate Immunity

PDF:Innate immunity

Video: https://www.youtube.com/watch?v=lbfywanF2Bc

Session 3: Adaptive Immunity

PDF:Adaptive immunity

Video:https://www.youtube.com/watch?v=PH190B74Hgo

Session 4: Cell mediated and humoral immunity

PDF:CMI and humoral

Video: https://www.youtube.com/watch?v=2vh24StylNo

Session 5: Cells and organs of immune system

PDF: Cells and organs

Video: https://www.youtube.com/watch?v=k9QAyP3bYmc

Session 6: Antigen

PDF Link: Antigen

YouTube Link: https://www.youtube.com/watch?v=sJptG3_uiVg

Session 7: Antibody

PDF: Antibody

Video:https://www.youtube.com/watch?v=zg61a3BoR8Y

Session 8: Antigenic determinants on immunoglobulins

PDF: Antigenic determinants

Video: https://www.youtube.com/watch?v=fa3nQGxaDO8

Session 9: MHC complex 

PDF: MHC

Video: https://www.youtube.com/watch?v=R69M7NuBNBA

Session 10: Antigen-antibody interactions

PDF: Antibody-Antigen Interaction

Video: https://www.youtube.com/watch?v=w5vPxsH2j8M

Session 11: Antigen-antibody reaction - Precipitin reactions, agglutination reactions

PDF: Precipitin reaction

Video:https://www.youtube.com/watch?v=Ew15yHuqzmY

Session 12: Specific and Non specific defence mechanism in Fish

PDF 1.  http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-V.pdf

PDF 2. http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-III.pdf

Video 1: https://www.youtube.com/watch?v=pi7LLW383k8&t=16s

Video 2: https://www.youtube.com/watch?v=os1oh8AprYw

Session 14: Vaccine

PDF 1: http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XIV.pdf

PDF 2: http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XV.pdf

Video 1: https://www.youtube.com/watch?v=YlKLpQEWILg&t=57s  

Video : https://www.youtube.com/watch?v=ccT8B37nYRI

Session 15: Serological methods

PDF:Serological test

Video:https://www.youtube.com/watch?v=W-cSTxUorFc

Practicals

Session 1: Collection, separation and identification of fish leucocytes

PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/1.-Isolation-of-fish-leucocytes.pdf

YouTube Link https://www.youtube.com/watch?v=NMVFvY3znBA

Session 3: Differential counting - RBC and WBC by Haemocytometer (Neubauer Chamber)

PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/3.Cell-count-RBCWBC.pdf

YouTube Link https://www.youtube.com/watch?v=MKS0KM3lr90

Our Main Teachers

Dr. Ngairangbam Sushila joined the University as an Assistant Professor in the department of Aquatic Animal  Health Management, School of Fisheries. She completed her doctoral programme from ICAR-Central Institute of Fisheries Education, Mumbai.

Teaching, Research and Product Development, Business Development.