An in depth study for better application of mind to further approach towards biology.
This course would make students well equipped with the process of body and its functioning.
Fundamentals of biochemistry
Teacher
PANKAJ MEHER
Category
Core Courses
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Course Name :FUNDAMENTALS OF BIOCHEMISTRY
Code(Credit) : CUTM1503(3-2-1)
Course Objectives
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Learning Outcomes
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Course Syllabus
Module-I
Carbohydrates: Structure and Biological importance of :Monosaccharides,Disaccharides,Polysaccharides and Glycoconjugates
Assignment- prepare a note on carbohydreate.
Practice
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Qualitative tests of functional groups in carbohydrates
Module-II
Lipids: Structure and Significance: Physiologically important saturated and unsaturated fatty acids,Tri-acylglycerols,Phospholipids,Glycolipids,Steroids
Assignment- prepare a note on lipids.
Practice:-
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Qualitative tests of functional groups in lipids.
Module-III
Proteins: Amino acids: Structure, Classification and General properties of α-amino acids;Physiological importance of essential and non-essential α-amino acidsProteins:Bonds stabilizing protein structure and Denaturation;Levels of organization in proteins;Introduction to simple and conjugate proteins
Assignment- prepare a note on prortein.
Practice:-
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Qualitative tests of functional groups in protein
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Separation of amino acid using Paper Chromatography.
Module-IV
Immunoglobulins: Basic Structure, Classes and Function, Antigenic Determinants.
Nucleic Acids: Structure: Purines and pyrimidines, Nucleosides, Nucleotides, Nucleic acids;Cot Curves: Base pairing, Denaturation and Renaturation of DNA;Types of DNA and RNA;Complementarity of DNA;Hpyo-Hyperchromaticity of DNA.
Assignment- prepare a note on nucleic acid.
Practice:-
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Quantitative estimation of salmon sperm/calf thymus DNA using colorimeter(Diphenylamine reagent) or spectrophotometer (A260 measurement)
Module-V
Enzymes: Nomenclature and classification;Cofactors;Specificity of enzyme action;Isozymes;Mechanism of enzyme action.
Assignment- prepare a note on Enzyme.
Practice:-
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Action of salivary amylase under optimum conditions.
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Effect of different pH on salivary amylase activity.
Module-VI
Enzyme kinetics;Factors affecting rate of enzyme-catalyzed reactions;Derivation of Michaelis-Menten equation;Concept of Km and Vmax, and Lineweaver-Burk plot;Multi- substrate reactions.
Assignment- prepare a note on feedback mechanism of enzyme.
Practice:-
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Demonstration of proteins separation by SDS-PAGE.
Module-VII
Enzyme inhibition;Allosteric enzymes and their kinetics;Regulation of enzyme action.
Assignment- prepare a note on allosteric enzyme.
practice:-
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Effect of different Temperature on salivary amylase activity.
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Effect of inhibitor on salivary amylase activity
Text Books:
Cox, M.M and NelsoEXT n, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition, W.H. Freeman and Co., New York.
Reference Books:
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Cox, M.M and NelsoEXT n, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition,
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W.H. Freeman and Co., New York.
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Berg, J.M., Tymoczko, J.L. and Stryer, L. (2007). Biochemistry, VI Edition, W.H. Freeman and Co., New York.rs
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Sources of Environmental hazards, hazard identification and accounting, fate of toxic and persistent substances in the environment, dose Response Evaluation, exposure Assessment.
Session 1
Carbohydrates: Structure and Biological importance of : Monosaccharides
session 5(Assignment)
prepare a note on lipids.
session 6(Practice)
Qualitative tests of functional groups in carbohydrates,
Session 7
Lipids: Structure and Significance:Physiologically important saturated and unsaturated fatty acids
Session 12(Assignment)
Prepare a note on prortein.
session 13(Practice)
- Qualitative tests of functional groups in lipids.
Session 14
Proteins: Amino acids:
Structure, Classification and General properties of α-amino acids
Session 15
Physiological importance of essential and non-essential α-amino acids Proteins
Session 16
Bonds stabilizing protein structure and Denaturation;
Session 17
Levels of organization in proteins;
https://www.youtube.com/watch?v=hok2hyED9go
Session 18
Introduction to simple and conjugate proteins
Session 19(Assignment)
Prepare a note on nucleic acid.
session 20
- Qualitative tests of functional groups in protein
http://vlab.amrita.edu/?sub=3&brch=63&sim=1094&cnt=1
- Separation of amino acid using Paper. Chromatography .
Session 21
Immunoglobulins: Basic Structure, Classes and Function of antibody, Antigenic Determinants.
Session 22
Nucleic Acids:
Structure: Purines and pyrimidines, Nucleosides, Nucleotides, Nucleic acids
Session 23
Cot Curves: Base pairing, Denaturation and Renaturation of DNA
Session 27(Assignment)
prepare a note on Enzyme.
Session 28 (Practice)
- Quantitative estimation of salmon sperm/calf thymus DNA using colorimeter(Diphenylamine reagent) or spectrophotometer (A260 measurement)
Session 33(Assignment)
Prepare a note on feedback mechanism of enzyme.
session 34(Practice)
Action of salivary amylase under optimum conditions.
https://www.youtube.com/watch?v=iaqxTaXit9M
Effect of different pH on salivary amylase activity.
Session 36
Factors affecting rate of enzyme-catalyzed reactions;
Session 37
Derivation of Michaelis-Menten equation and Concept of Km and Vmax, Lineweaver-Burk plot;
https://www.youtube.com/watch?v=8PWF5OeB7Ec
session 39(Assignment)
- Demonstration of proteins separation by SDS-PAGE
Session 40(Practice)
- Demonstration of proteins separation by SDS-PAGE
Session 42
Allosteric enzymes and their kinetics and Regulation of enzyme action
Session 43(Assignment)
prepare a note on allosteric enzyme
Session 44(Practice)
Effect of different Temperature on salivary amylase activity.
https://www.youtube.com/watch?v=iaqxTaXit9M
Effect of inhibitor on salivary amylase activity
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