Fundamentals of Plant Biotechnology
Teacher
Dr. Koustava Kumar Panda
Category
Core Courses
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Code(Credit) : ASPB 2201 (1-1-0)
Course Objectives
- 1. To familiarize the students with the key developments in the sphere of Plant Biotechnology.
- 2. To achieve self sufficiency in the area of production of important crop species through plant biotechnology.
- 3. To make the students aquainted with the widely exploited techniques in plant molecular biology.
CO-PO Mapping
COs Course outcomes Mapping of COs with POs
CO1 Knowledge enhancement of students
in the sphere of plant biotechnology
by understanding the concepts, principle and practices.
CO2 Professional development and instigating
entrepreneurship skills in the area of
plant tissue culture.
CO3 Understanding and application of various
molecular techniques in the area of
plant biotechnology.
Course Syllabus
Theory:
Concepts and applications of plant biotechnology: Scope, organ culture, embryo culture, cell suspension culture, callus culture, anther culture, pollen culture and ovule culture and their applications; Micro-propagation methods; organogenesis and embryogenesis, Synthetic seeds and their significance; Embryo rescue and its significance; somatic hybridization and cybrids; Somaclonal variation and its use in crop improvement; cryo-preservation; Introduction to recombinant DNA methods: physical (Gene gun method), chemical (PEG mediated) and Agrobacterium mediated gene transfer methods; Transgenics and its importance in crop improvement; PCR techniques and its applications; RFLP, RAPD, SSR; Marker Assisted Breeding in crop improvement; Biotechnology regulations.
Practical:
Composition of various tissue culture media and preparation of stock solutions for MS nutrient medium, Callus induction from various explants, Micro-propagation, hardening and acclimatization, Demonstration on isolation of DNA, Demonstration of gel electrophoresis techniques and DNA finger printing.
THEORY
1. Scope and importance of Plant Biotechnology, importance of plant tissue culture
2. Types of cultures carried out under plant tissue culture and their importance
3. Callus culture, organ culture, embryo culture and their importance
4. Cell Suspension culture, Anther culture, Pollen culture, Ovule culture: importance of carrying out such cultures
5. Synthetic seeds: Production and their significance
6. Embryo rescue and its significance
7. Somatic hybridization: Protoplast isolation, Fusion, selection of somatic hybrids, Cybrids and their role
8. Somaclonal variation and its role in crop improvement
9. Cryopreservation: Importance and the role of cryoprotectants
10. Recombinant DNA Technology/Gene cloning: Steps involved and its applications
11. Vector based and vector-less approaches for gene delivery
12. Transgenic plants as potential bioreactors
13. PCR technique and its applications
14. Molecular markers (RFLP, RAPD, SSR) and their roles in biotechnology
15. Marker assisted selection for mapping agronomic traits
16. Guidelines to be followed with respect to genetic engineering practices, role of different organizations like DBT, RCGM, GEAC, RDAC in monitoring genetic engineering practices
LABORATORY
- Organization of plant tissue culture laboratory and its maintainance
- Approaches for Sterilization
- Preparation of stock solutions for MS nutrient medium
- Preparation of MS medium and its sterilization
- Inoculation of explants into the sterilized media
- Isolation of plant genomic DNA and its spectrophotometric quantification
- Demonstration of agarose gel electrophoresis technique
- Demonstration of PCR technique
- Separation of DNA by agarose gel electrophoresis
- Study of the parts and functions of different equipments used in biological research
- Maintainance of invitro raised plants in polyhouse through a control on the factors contributing to plant growth
- Study on the use of Agrobacterium as a vector for gene delivery
References:
- Introduction to Plant Biotechnology by H.S.Chawla
- Biotechnology-Expanding Horizons by B.D.Singh
- Genetic Engineering by Preeti Joshi
- Principles of Gene Manipulation by Primrose and Twymann
- Molecular Biotechnology by Bernard R. Glick and Jack J. Pasternak
- Lehninger Principles of Biochemistry by David L. Nelson & Michael M. Cox
- Molecular Cloning by Sambrook and Russel
- Practical Biotechnology: Principles and Protocols by Hrudayanath Thatoi, Supriya Dash & Swagat Kumar Das
Session Plan
Session 1
Historical developments in Plant Biotechnology along with the scopes offered by it
https://www.slideshare.net/prakashsiju/chapter-3-plant-tissue-culture
https://youtu.be/6y13hYGPi8Q
The session introduces one to the historical developments in Plant Biotechnology along with the scopes offered by it
Provides information on different types of cultures including protoplast culture, callus culture, organ culture, embryo culture, cell suspension culture, anther culture, pollen culture, ovule culture and their significance.
Session 3
Embryo rescue and its significance
https://www.slideshare.net/RIZWANABBAS3/embryo-culture-application-
Provides information on rescuing the embryos of wide crosses and also the protection of embryos in unfavourable environments.
Session 4
Somatic hybridization and its importance
https://www.slideshare.net/neha_ag/somatic-hybridization-67740744
https://www.slideshare.net/devhingra/somatic-hybridization-60180584
Provides information on raising plants through somatic hybridization for producing desirable products
Session 5
Micropropagation in tissue culture
https://www.slideshare.net/SonamkzBhutia/micro-propagation
Provides information on the exploitation of the technique to raise commercially important plants
Session 6
Somaclonal variation and its role in crop improvement
https://www.slideshare.net/RakeshKumar440/somaclonal-variation-26537134
Provides information on the importance of somaclonal variants in tissue cullture and plant breeding
Session 7
Synthetic seeds: Production and their significance
https://www.slideshare.net/SonamkzBhutia/artificial-seed-98432308
Provides detailed information on the production of synthetic seeds and their importance in the sphere of plant science
Session 8
Steps involved in gene cloning
https://www.slideshare.net/gohilsanjay3/gene-cloning-principles-an-technique
Provides information on the importance of cloning desirable genes to produce superior genotypes
Session 9
Vector based approaches for gene delivery
https://www.slideshare.net/NISHANTHSEKAR1/agrobacterium-mediated-gene-transfer-77002058
Provides information on the use of potential vectors for the transfer of genes
Session 10
Vectorless approaches for gene delivery
https://www.slideshare.net/manjeshsaakre/vector-less-gene-transfer
Provides information on the use of different direct gene delivery methods
Session 11
Polymerase chain reaction and its applications
https://www.slideshare.net/subramaniansethupath/polymerase-chain-reaction-principles-and-practice
provides information on the principle and applications of PCR
Session 12
Molecular markers: Construction and uses
https://www.slideshare.net/VikasSingh639/molecular-markers-75788487
Provides information on the role of markers in Plant biotechnology and Genomics
Session 13
Organizations involved in monitoring genetic engineering practises
https://www.slideshare.net/Adrienna/national-and-international-regulatory-framework-for-gm-crops2015
Provides detailed information on the organizations involved in monitoring genetic engineering practices and the ethical issues to be taken care of
Session 14
Organization of plant tissue culture laboratory
Provides detailed idea on the general set up of a plant tissue culture laboratory and its purpose
Session 15
Approaches for sterilization
Imparts information on the various approaches for sterilization and their importance
Session 16
Preparation of stock solutions of MS nutrient medium
Session 17
Preparation of MS nutrient medium and its sterilization
Session 19
Isolation of plant genomic DNA and its spectrophotometric quantification
Session 22
Visit to high tech polyhouse for studying the factors contributing to plant growth
Our Main Teachers
I am Dr. Koustava Kumar Panda presently working as an Associate Professor in the department of Plant Biotechnology, M.S.Swaminathan School of Agriculture, Centurion university, Paralakhemundi since July 2017. I have worked on the the nutritional profiling of prominent rice landraces of Southern Odisha on the elemental, mineral, nurtitional quality attributes in addition to studying their […]
Dr. Satyabrata Nanda has a Ph.D. in Biotechnology with a specialization in Plant Protection. His education includes B-Tech, M-Tech, and Ph.D. in Biotechnology. He’s received the Overseas Postdoctoral Fellowship from the Chinese Academy of Agricultural Sciences for research at China National Rice Research Institute. His research is primarily focused on plant protection by using functional […]