Fundamentals of Plant Biotechnology

Teacher

Dr. Koustava Kumar Panda

Category

Core Courses

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Course Name : Fundamentals of Plant Biotechnology

Code(Credit) : ASPB 2201 (1-1-0)

Course Objectives

  • Familiarize the students with the key developments in the sphere of Plant Biotechnology.
  • Train students with the techniques associated with the invitro propagation of plants and their maintainance.
  • Create awareness on the importance of plant diversity and its conservation both insitu and exsitu.
  • Train students on basic molecular biology techniques.

Outcomes

  • The students do understand the importance of plant diversity and their conservation through invitro propagation and maintainance.
  • The students get aquainted with the widely exploited techniques in molecular biology like isolation of plant genomic DNA, their separation by gel electrophoresis, amplification of separated DNA by polymerase chain reaction, construction of phylogenetic trees to study genetic relatedness, construction of genome maps using markers.
  • Gain insight into genetic engineering techniques and the importance of using GMOs as bioreactors for the inexpensive production of pharmaceuticals and nutraceuticals.
  • Marker assisted selection for mapping agriculturally important traits in crop plants.

Course Syllabus

THEORY

1. Scopes offered by Plant Biotechnology, Importance of Plant Tissue Culture;
2. Different types of cultures carried out under Plant Tissue Culture and their importance;
3. Callus culture, organ culture, embryo culture and their importance;
4. Cell Suspension culture, Anther culture, Pollen culture, Ovule culture: Importance of carrying out such cultures;
5. Synthetic seeds: Production and their significance;
6. Embryo rescue and its significance;
7. Somatic hybridization: Protoplast isolation, Fusion, selection of somatic hybrids, Cybrids and their role;
8. Somaclonal Variation and its role in crop improvement;
9. Cryopreservation: Importance and the role of Cryoprotectants;
10. Recombinant DNA Technology/Gene cloning: Steps involved ;
11. Vector based and vector-less approaches for gene delivery;
12. Transgenic Plants as Potential bioreactors;
13. PCR technique and its applications;
14. Markers (RFLP, RAPD, SSR) and their roles in Agricultural biotechnology;
15. Marker assisted selection for mapping agronomic traits;
16. Guidelines to be followed with respect to genetic engineering practices, role of different organizations like DBT, GEAC, in monitoring genetic engineering practices;

 

LABORATORY

  • Organization of Plant Tissue Culture Laboratory;
  • Approaches for Sterilization;
  • Preparation of stock solutions of MS nutrient medium;
  • Preparation of MS medium and its sterilization;
  • Inoculation of Explants into the sterilized media;
  • Isolation of Plant Genomic DNA and its spectrophotometric quantification;
  • Demonstration of Agarose gel electrophoresis technique;
  • Demonstration of PCR technique;
  • Separation of DNA by Agarose gel electrophoresis;
  • Study of the parts and functions of different equipments used in Biotechnology;
  • Visit to high tech poly house for a study of factors contributing to plant growth;
  • Agrobacterium tumefaciens mediated plant transformation

Session Plan

Session 1
Historical developments in Plant Biotechnology along with the scopes offered by it

https://www.slideshare.net/prakashsiju/chapter-3-plant-tissue-culture

https://youtu.be/6y13hYGPi8Q

The session introduces one to the historical developments in Plant Biotechnology along with the scopes offered by it

protoplast culture, callus culture, organ culture, embryo culture, cell suspension culture, anther culture, pollen culture, ovule culture and their importance

Session 6
Somaclonal variation and its role in crop improvement

https://www.slideshare.net/RakeshKumar440/somaclonal-variation-26537134

https://youtu.be/pFwfn08KwIY

Session 7
Synthetic seeds: Production and their significance

https://www.slideshare.net/SonamkzBhutia/artificial-seed-98432308

https://youtu.be/M4GjsRvHHYw

 

Session 14
Organization of plant tissue culture laboratory

https://youtu.be/TORRxwbz7aY

Session 15
Approaches for sterilization

 

https://youtu.be/xjYdOcT6s1Y

Session 16
Preparation of stock solutions of MS nutrient medium

https://youtu.be/eMv_PMNPYMc

Session 17
Preparation of MS nutrient medium and its sterilization

https://youtu.be/ltbdM3boWmU

Session 18

Inoculation of explants into sterilized media

https://youtu.be/zd0iVJrQwyY

Session 19

Isolation of plant genomic DNA and its spectrophotometric quantification

 

https://youtu.be/9-oIhcH4Nl4

https://youtu.be/oIlGK5uWH9o

https://youtu.be/p2RARQj0X9Y

Session 20

Separation of DNA by agarose gel electrophoresis

 

https://youtu.be/vq759wKCCUQ

Session 21

Demonstration of PCR technique

 

https://youtu.be/matsiHSuoOw

Session 22

Visit to high tech polyhouse for studying the factors contributing to plant growth

 

https://youtu.be/9Qz5CClJHH8

Session 23

Construction of RAPD molecular marker

 

https://youtu.be/8pvOag1WaQ8

Case Studies

Case Studies

Our Main Teachers

I am Dr. Koustava Kumar Panda presently working as an Associate Professor in the department of Plant Biotechnology, M.S.Swaminathan School of Agriculture, Centurion university, Paralakhemundi since July 2017. I have worked on the the nutritional profiling of prominent rice landraces of Southern Odisha on the elemental, mineral, nurtitional quality attributes in addition to studying their […]