Fundamentals of Plant Biotechnology

Teacher

Dr. Koustava Kumar Panda

Category

Core Courses

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Course Name : Fundamentals of Plant Biotechnology

Code(Credit) : ASPB 2201 (1-1-0)

Course Objectives

  • 1. To familiarize the students with the key developments in the sphere of Plant Biotechnology.
  • 2. To achieve self sufficiency in the area of production of important crop species through plant biotechnology.
  • 3. To make the students aquainted with the widely exploited techniques in plant molecular biology. 

CO-PO Mapping

COs               Course outcomes                             Mapping of COs with POs

 

CO1               Knowledge enhancement of students

                        in the sphere of plant biotechnology

                         by understanding the concepts, principle and                                                               practices.  

CO2              Professional development and instigating

                      entrepreneurship skills in the area of

                       plant tissue culture. 

CO3              Understanding and application of various

molecular techniques in the area of

plant biotechnology.

Course Syllabus

Theory:

Concepts and applications of plant biotechnology: Scope, organ culture, embryo culture, cell suspension culture, callus culture, anther culture, pollen culture and ovule culture and their applications; Micro-propagation methods; organogenesis and embryogenesis, Synthetic seeds and their significance; Embryo rescue and its significance; somatic hybridization and cybrids; Somaclonal variation and its use in crop improvement; cryo-preservation; Introduction to recombinant DNA methods: physical (Gene gun method), chemical (PEG mediated) and Agrobacterium mediated gene transfer methods; Transgenics and its importance in crop improvement; PCR techniques and its applications; RFLP, RAPD, SSR; Marker Assisted Breeding in crop improvement; Biotechnology regulations.

Practical:

Composition of various tissue culture media and preparation of stock solutions for MS nutrient medium, Callus induction from various explants, Micro-propagation, hardening and acclimatization, Demonstration on isolation of DNA, Demonstration of gel electrophoresis techniques and DNA finger printing.

THEORY

1. Scope and importance of Plant Biotechnology, importance of plant tissue culture
2. Types of cultures carried out under plant tissue culture and their importance

3. Callus culture, organ culture, embryo culture and their importance
4. Cell Suspension culture, Anther culture, Pollen culture, Ovule culture: importance of carrying out such cultures
5. Synthetic seeds: Production and their significance
6. Embryo rescue and its significance
7. Somatic hybridization: Protoplast isolation, Fusion, selection of somatic hybrids, Cybrids and their role
8. Somaclonal variation and its role in crop improvement
9. Cryopreservation: Importance and the role of cryoprotectants
10. Recombinant DNA Technology/Gene cloning: Steps involved and its applications
11. Vector based and vector-less approaches for gene delivery
12. Transgenic plants as potential bioreactors
13. PCR technique and its applications
14. Molecular markers (RFLP, RAPD, SSR) and their roles in biotechnology
15. Marker assisted selection for mapping agronomic traits
16. Guidelines to be followed with respect to genetic engineering practices, role of different organizations like DBT, RCGM, GEAC, RDAC in monitoring genetic engineering practices

 

LABORATORY

  1. Organization of plant tissue culture laboratory and its maintainance
  2. Approaches for Sterilization
  3. Preparation of stock solutions for MS nutrient medium
  4. Preparation of MS medium and its sterilization
  5. Inoculation of explants into the sterilized media
  6. Isolation of plant genomic DNA and its spectrophotometric quantification
  7. Demonstration of agarose gel electrophoresis technique
  8. Demonstration of PCR technique
  9. Separation of DNA by agarose gel electrophoresis
  10. Study of the parts and functions of different equipments used in biological research
  11. Maintainance of invitro raised plants in polyhouse through a control on the factors contributing to plant growth
  12. Study on the use of Agrobacterium as a vector for gene delivery

References:

  1. Introduction to Plant Biotechnology by H.S.Chawla
  2. Biotechnology-Expanding Horizons by B.D.Singh
  3. Genetic Engineering by Preeti Joshi
  4. Principles of Gene Manipulation by Primrose and Twymann
  5. Molecular Biotechnology by Bernard R. Glick and Jack J. Pasternak
  6. Lehninger Principles of Biochemistry by David L. Nelson & Michael M. Cox
  7. Molecular Cloning by Sambrook and Russel
  8. Practical Biotechnology: Principles and Protocols by Hrudayanath Thatoi, Supriya Dash & Swagat Kumar Das

Session Plan

Session 1
Historical developments in Plant Biotechnology along with the scopes offered by it

https://www.slideshare.net/prakashsiju/chapter-3-plant-tissue-culture

https://youtu.be/6y13hYGPi8Q

The session introduces one to the historical developments in Plant Biotechnology along with the scopes offered by it

Provides information on different types of cultures including protoplast culture, callus culture, organ culture, embryo culture, cell suspension culture, anther culture, pollen culture, ovule culture and their significance.

Session 3
Embryo rescue and its significance

https://www.slideshare.net/RIZWANABBAS3/embryo-culture-application-

embryo-rescue

Provides information on rescuing the embryos of wide crosses and also the protection of embryos in unfavourable environments.

https://youtu.be/bi1kCxtrzlI

Session 4
Somatic hybridization and its importance

https://www.slideshare.net/neha_ag/somatic-hybridization-67740744

https://www.slideshare.net/devhingra/somatic-hybridization-60180584

Provides information on raising plants through somatic hybridization for producing desirable products

https://youtu.be/hfNSiB0fW64

Session 5
Micropropagation in tissue culture

https://www.slideshare.net/SonamkzBhutia/micro-propagation

https://youtu.be/ykKs5icYwq0

Provides information on the exploitation of the technique to raise commercially important plants

 

Session 6
Somaclonal variation and its role in crop improvement

https://www.slideshare.net/RakeshKumar440/somaclonal-variation-26537134

https://youtu.be/pFwfn08KwIY

Provides information on the importance of somaclonal variants in tissue cullture and plant breeding

Session 7
Synthetic seeds: Production and their significance

https://www.slideshare.net/SonamkzBhutia/artificial-seed-98432308

https://youtu.be/M4GjsRvHHYw

Provides detailed information on the production of synthetic seeds and their importance in the sphere of plant science

Session 8
Steps involved in gene cloning

https://www.slideshare.net/gohilsanjay3/gene-cloning-principles-an-technique

https://youtu.be/3YRjpiBqSU4

Provides information on the importance of cloning desirable genes to produce superior genotypes

Session 9
Vector based approaches for gene delivery

https://www.slideshare.net/NISHANTHSEKAR1/agrobacterium-mediated-gene-transfer-77002058

Provides information on the use of potential vectors for the transfer of genes

https://youtu.be/yesNHd9h8k0

 

Session 10
Vectorless approaches for gene delivery

https://www.slideshare.net/manjeshsaakre/vector-less-gene-transfer

https://youtu.be/_-apTP71mXs

Provides information on the use of different direct gene delivery methods

Session 11
Polymerase chain reaction and its applications

https://www.slideshare.net/subramaniansethupath/polymerase-chain-reaction-principles-and-practice

provides information on the principle and applications of PCR

https://youtu.be/QwT-Tj89VLo

Session 12
Molecular markers: Construction and uses

https://www.slideshare.net/VikasSingh639/molecular-markers-75788487

https://youtu.be/Quk-Dh65iHY

Provides information on the role of markers in Plant biotechnology and Genomics

https://youtu.be/UhxwFm7izBY

Session 13
Organizations involved in monitoring genetic engineering practises

https://www.slideshare.net/Adrienna/national-and-international-regulatory-framework-for-gm-crops2015

Provides detailed information on the organizations involved in monitoring genetic engineering practices and the ethical issues to be taken care of

https://youtu.be/jAhjPd4uNFY

Session 14
Organization of plant tissue culture laboratory

https://youtu.be/TORRxwbz7aY

Provides detailed idea on the general set up of a plant tissue culture laboratory and its purpose

Session 15
Approaches for sterilization

Imparts information on the various approaches for sterilization and their importance

 

https://youtu.be/xjYdOcT6s1Y

Session 16
Preparation of stock solutions of MS nutrient medium

https://youtu.be/eMv_PMNPYMc

Session 17
Preparation of MS nutrient medium and its sterilization

https://youtu.be/ltbdM3boWmU

Session 18

Inoculation of explants into sterilized media

https://youtu.be/zd0iVJrQwyY

Session 19

Isolation of plant genomic DNA and its spectrophotometric quantification

 

https://youtu.be/9-oIhcH4Nl4

https://youtu.be/oIlGK5uWH9o

https://youtu.be/p2RARQj0X9Y

Session 20

Separation of DNA by agarose gel electrophoresis

 

https://youtu.be/vq759wKCCUQ

Session 21

Demonstration of PCR technique

 

https://youtu.be/matsiHSuoOw

Session 22

Visit to high tech polyhouse for studying the factors contributing to plant growth

 

https://youtu.be/9Qz5CClJHH8

Session 23

Construction of RAPD molecular marker

 

https://youtu.be/8pvOag1WaQ8

Our Main Teachers

I am Dr. Koustava Kumar Panda presently working as an Associate Professor in the department of Plant Biotechnology, M.S.Swaminathan School of Agriculture, Centurion university, Paralakhemundi since July 2017. I have worked on the the nutritional profiling of prominent rice landraces of Southern Odisha on the elemental, mineral, nurtitional quality attributes in addition to studying their […]

Dr. Satyabrata Nanda

Associate Professor & Head, Dept. of Biotechnology
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Dr. Satyabrata Nanda has a Ph.D. in Biotechnology with a specialization in Plant Protection. His education includes B-Tech, M-Tech, and Ph.D. in Biotechnology. He’s received the Overseas Postdoctoral Fellowship from the Chinese Academy of Agricultural Sciences for research at China National Rice Research Institute. His research is primarily focused on plant protection by using functional […]