General Microbiology
Teacher
Dr. Monali Priyadarsini Mishra
Category
Core Courses
Course Attendees
Still no participant
Course Reviews
Still no reviews
Course Name : CUTM1711-General Microbiology
Code(Credit) : (3-1-0)
Course Objectives
- To know various Culture media and their applications and also understand various physical and chemical means of sterilization
- To know General bacteriology and microbial techniques for isolation of pure cultures of bacteria, fungi and virus
- To master aseptic techniques and be able to perform routine culture handling tasks safely and effectively
Learning Outcomes
- This study demonstrates the theory and practical skills in microscopy and their handling techniques and staining procedures.
- Understanding the details of microbial cell organelles.
- Provides knowledge on the growth of microorganism.
- Provides knowledge culturing microorganism.
Course Syllabus
Module-1
History and scope of Microbiology, Recent trends and developments in modern microbiology. Identification, characterization and classification of microorganisms. Distinguishing characteristics between prokaryotic and eukaryotic cells. Structure and function of Cell wall of bacteria, cell membranes, flagella, pili, capsule, gas vesicles, carboxysomes, magnetosomes and phycobolisomes.
Practice:
- Demonstration the different type of Sterilization technique and operation of the Instruments used in microbiological lab.
- Demonstration of various parts of microscope its functioning and care.
Module-2
Methods of sterilization: Physical methods – Dry heat, moist heat, radiation methods, filtration methods, chemical methods and their application. Concept of containment facility, sterilization at industrial level. Different staining techniques used in bacteriology.
Practice:
- Preparation of bacterial smear and staining – Gram’s, Acid-fast, Staining of bacterial spores flagella, capsule, spirochaetes
- Preparation of media, cultivation of bacteria
Module-3
Bacterial nutrition – Nutritional requirement of bacteria. Cultivation of aerobes and anaerobes, Reproduction in bacteria and spore formation. Bacterial growth curve and bacterial nutrition Media. Culture Media, Liquid and solid media, defined and synthetic media, routine laboratory media (basal, enriched, selective, enrichment, indicator, and transport media).
Practice:
- Biochemical tests for identification of bacteria
- Preservation of stock cultures of bacteria
Suggested References
- Textbook of Microbiology- Ananthanarayan & Paniker (10th Ed)
- Medical Microbiology-by Fritz H. Kayser et al
- Medical Laboratory Technology by Kanai Lal Mukherjee, Publisher Tata McGrawHill
- Microbiology (7th Ed)- by Prescott
- Practical Book of Medical Microbiology by Satish Gupta, Publisher JaypeeBrothers
- Medical Laboratory Manual for Tropical Countries Vol. I and II by Monica Cheesbrough
- Essential Medical Microbiology- by Rajesh Bhatia (4th Ed)
- Clinical laboratory methods and diagnosis by Gradwohls, 2000, Publisher Mosby
- Medical laboratory science theory and practice, J Ochei and Kolhatkar, 2002, publisher TBS
Session Plan
Session 1
History and scope of Microbiology
Video 1: https://www.youtube.com/watch?v=hvvchbfw8-k
Video 2: https://www.youtube.com/watch?v=0vr2fcJZtdA
Presentation: https://www.slideshare.net/SalmanAli83/historyintroduction-scope-of-microbiology-siws
Session 2
Recent trends and developments in modern microbiology
Presentation 1: https://www.slideshare.net/doctorrao/trends-in-medical-microbiology-series-1
Presentation 2:https://www.slideshare.net/ksitara/current-trends-and-opportunities-in-microbiological-researchs
Session 3
Identification of microorganisms
Video: https://www.youtube.com/watch?v=cMVyrrdgaYk
Presentation: https://www.slideshare.net/doctortvrao/basics-in-identification-of-bacteria-by-drtvrao-md
Session 4
characterization of microorganisms
Video: https://www.youtube.com/watch?v=jgecAM_y0H8
Presentation: https://www.slideshare.net/sayantanmondal96/identification-of-bacteria-35638850
Session 5
classification of microorganisms
Video: https://www.youtube.com/watch?v=iDVq2etiknU
Presentation: https://www.slideshare.net/mgcnkedahsc/11-classsification-of-microorganisms
Session 6
classification of microorganisms
Video 1 : https://www.youtube.com/watch?v=SgEkbDoPc5Y
Video 2 : https://www.youtube.com/watch?v=8IJRzcPC9wg
Presentation: https://www.slideshare.net/AhmadFaridAzizi/classification-of-microorganisms-lecture-2
Session 7
Distinguishing characteristics between prokaryotic and eukaryotic cells
Video 1: https://www.youtube.com/watch?v=RQ-SMCmWB1s
Video 2:https://www.youtube.com/watch?v=9o6huiw7u5o
Presentation: https://www.slideshare.net/EasyBiologyClassEBC/difference-between-prokaryotes-and-eukaryotes-ppt-by-easybiologyclass
Session 8
Structure and function of Cell wall of bacteria
Video : https://www.youtube.com/watch?v=VkVLFbg0z5k
Presentation: https://www.slideshare.net/rajpalchoudharyjat/cell-wall-structure-and-function
Session 9
Structure and function of Cell wall of bacteria
Video: https://www.youtube.com/watch?v=ei6Z7orCpPk
Presentation: https://www.slideshare.net/AshfaqAhmad52/bacterial-cell-wall-67925149
Session 10
cell membranes, flagella, pili
Video 1: https://www.youtube.com/watch?v=r7CL0NwP0qM
Video 2: https://www.youtube.com/watch?v=VzqC7nag6KM
Presentation: https://www.slideshare.net/gohilsanjay3/structure-of-bacterial-cell-108802472
Session 11
capsule, gas vesicles, carboxysomes
Video 1: https://www.youtube.com/watch?v=89zOOFan_Ok
Video 2:https://www.youtube.com/watch?v=MJPtqcBE9I0
Presentation: https://www.slideshare.net/arijabuhaniyeh/chapter-3-microbiology-8th-edition
Session 12
magnetosomes and phycobolisomes
Video : https://www.youtube.com/watch?v=_FdIpS0IZLc
Presentation : https://www.slideshare.net/CAS0609/bacterial-magnetosomes-microbiology-biomineralization-and-biotechnological-applications
Practice:
Preparation of bacterial smear and staining – Gram’s, Acid-fast, Staining of bacterial spores flagella, capsule, spirochaetes
Video 1: https://www.youtube.com/watch?v=c2BPE7wIK20
Video 2:https://www.youtube.com/watch?v=sxa46xKfIOY
Video 3:https://www.youtube.com/watch?v=NmfygqRQdeA
Video 4:https://www.youtube.com/watch?v=0yttbfBsSgk
Practice:
Demonstration of various parts of microscope its functioning and care.
Session 13
Methods of sterilization
Video: https://www.youtube.com/watch?v=lmbvY-z1zt0
Presentation:
https://www.slideshare.net/saivivekkosaraju/sterilization-methods-32401134
Session 14
Physical methods – Dry heat
Video: https://www.youtube.com/watch?v=vvFDypILkTA
Presentation: https://www.slideshare.net/doctortvrao/sterilization-physical-methods
Session 15
moist heat
Video: https://www.youtube.com/watch?v=j23s_cxwxSE
Presentation: https://www.slideshare.net/zionpattres/moist-heat-sterilization
Session 16
radiation methods
Video: https://www.youtube.com/watch?v=t82WNvintDI
Presentation: http://large.stanford.edu/courses/2018/ph241/goronzy2/
Session 17
Session 18
chemical methods and their application
Video: https://www.youtube.com/watch?v=A31K7B9EEYU
Presentation:https://www.slideshare.net/alubajessabeth/chemical-agents-of-sterilization
Session 19
chemical methods and their application
Video: https://www.youtube.com/watch?v=ujVE4R5vXtA
Presentation: https://pacificbiolabs.com/chemical-sterilization
Session 20
Concept of containment facility
Video: https://www.youtube.com/watch?v=2NtBN-IOSDg
Presentation: https://www.slideshare.net/drmalathi13/biosafety-47467550
Session 21
sterilization at industrial level
Video: https://www.youtube.com/watch?v=NtepO6euSdY
Presentation: https://www.slideshare.net/NileshUtpure/industrial-sterilization
Session 22
sterilization at industrial level
Video: https://www.youtube.com/watch?v=UuX_HmSr8b8
Presentation: https://www.slideshare.net/vaishalijamdhade/industrial-sterilization-83030875
Session 23
Different staining techniques used in bacteriology
Video 1: https://www.youtube.com/watch?v=c2BPE7wIK20
Video 2:https://www.youtube.com/watch?v=sxa46xKfIOY
Video 3:https://www.youtube.com/watch?v=NmfygqRQdeA
Presentation: https://www.slideshare.net/shyleshmurthy/staining-techniques-and-types-procedure-100430921
Session 24
Different staining techniques used in bacteriology
Video 1:https://www.youtube.com/watch?v=0yttbfBsSgk
Video 2:https://www.youtube.com/watch?v=mZGEhql02_I
Video 3: https://www.youtube.com/watch?v=cXYfT5hSLoQ
Presentation: https://www.slideshare.net/doctorrao/bacterial-staining-methods
Practice:
Demonstration the different type of Sterilization technique and operation of the Instruments used in microbiological lab.
Video 1: https://www.youtube.com/watch?v=Dq5JgsxJpTY
Practice :
Preparation of media, cultivation of bacteria
Video 1: https://www.youtube.com/watch?v=cneascR3OEc&t=14€7s
Session 25
Bacterial nutrition – Nutritional requirement of bacteria
Video: https://www.youtube.com/watch?v=jia7avWv4s4
Presentation: https://www.slideshare.net/RIZWANABBAS3/bacterial-nutrition-and-growth
Session 26
Bacterial nutrition – Nutritional requirement of bacteria
Video: https://www.youtube.com/watch?v=O9yRDmUxlp8
Presentation: https://www.slideshare.net/RIZWANABBAS3/bacterial-nutrition-and-growth
Session 27
Cultivation of aerobes
Video: https://www.youtube.com/watch?v=dfmon5w1ZJI
Presentation: https://www.slideshare.net/NimishaK4/cultivation-of-bacteria-ppt-nimisha
Session 28
Cultivation of anaerobes
Video: https://www.youtube.com/watch?v=aFDYx-7ceS8
Presentation: https://www.slideshare.net/doctorrao/anaerobic-culture-methods
Session 29
Reproduction in bacteria
Video: https://www.youtube.com/watch?v=7sZ5Nz8_cfc
Presentation: https://www.slideshare.net/gohilsanjay3/reproduction-in-bacteria
Session 30
Reproduction in bacteria
Video: https://www.youtube.com/watch?v=n7Z5-mRB_gI
Presentation: https://www.slideshare.net/gohilsanjay3/reproduction-in-bacteria
Session 31
spore formation
Video: https://www.youtube.com/watch?v=NAcowliknPs
Presentation: https://www.slideshare.net/drKarthikvet/bacterial-spore
Session 32
spore formation
Video: https://www.youtube.com/watch?v=nwtEB-qKq3o
Presentation: https://www.slideshare.net/drKarthikvet/bacterial-spore
Session 33
Bacterial growth curve
Video: https://www.youtube.com/watch?v=BsPKcHShGYw
Presentation: https://www.slideshare.net/9426401633/bacterial-growth-curves
Session 34
bacterial nutrition Media
Video: https://www.youtube.com/watch?v=SQC8_65auw8&t=448s
Presentation: https://www.slideshare.net/HiwrHastear/types-of-culture-media
Session 35
Session 36
Sessions 37
Session 38
defined and synthetic media
Session 39
routine laboratory media (basal, enriched)
Video: https://www.youtube.com/watch?v=suNU9xEHoDs
Presentation: https://www.slideshare.net/sasiprasad/culture-methods-9213006
Session 40
selective, enrichment, media
Video: https://www.youtube.com/watch?v=EYHWgWiR6mU
Presentation: https://www.slideshare.net/musakhan9216/culture-media-used-in-microbiology
Session 41
indicator, and transport media
Video 1: https://www.youtube.com/watch?v=fpnR0L-TCXY
Video 2: https://www.youtube.com/watch?v=gYonmljfSX0
Presentation: https://www.slideshare.net/romagoyal37/culture-media-and-methods-for-mbbs
Practice:
Biochemical tests for identification of bacteria
Practice:
Preservation of stock cultures of bacteria
Case Studies
Case Studies
Our Main Teachers
Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]