General Microbiology

Teacher

Dr. Monali Priyadarsini Mishra

Category

Core Courses

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Course Name : General Microbiology

Code(Credit) : (3-1-0)

Course Objectives

  • To know various Culture media and their applications and also understand various physical and chemical means of sterilization
  • To know General bacteriology and microbial techniques for isolation of pure cultures of bacteria, fungi and virus
  • To master aseptic techniques and be able to perform routine culture handling tasks safely and effectively

Learning Outcomes

  • This study demonstrates the theory and practical skills in microscopy and their handling techniques and staining procedures.
  • Understanding the details of microbial cell organelles.
  • Provides knowledge on the growth of microorganism.
  • Provides knowledge culturing microorganism.

Course Syllabus

Module-1 

History and scope of Microbiology, Recent trends and developments in modern microbiology. Identification, characterization and classification of microorganisms. Distinguishing characteristics between prokaryotic and eukaryotic cells. Structure and function of Cell wall of bacteria, cell membranes, flagella, pili, capsule, gas vesicles, carboxysomes, magnetosomes and phycobolisomes. 

Practice:

  1. Demonstration the different type of Sterilization technique and operation of the Instruments used in microbiological lab.
  2. Demonstration of various parts of microscope its functioning and care.

Module-2 

Methods of sterilization: Physical methods – Dry heat, moist heat, radiation methods, filtration methods, chemical methods and their application. Concept of containment facility, sterilization at industrial level. Different staining techniques used in bacteriology.

Practice:

  1. Preparation of bacterial smear and staining – Gram’s, Acid-fast, Staining of bacterial spores flagella, capsule, spirochaetes
  2. Preparation of media, cultivation of bacteria

Module-3 

Bacterial nutrition – Nutritional requirement of bacteria. Cultivation of aerobes and anaerobes, Reproduction in bacteria and spore formation. Bacterial growth curve and bacterial nutrition Media. Culture Media, Liquid and solid media, defined and synthetic media, routine laboratory media (basal, enriched, selective, enrichment, indicator, and transport media).

Practice:

  1. Biochemical tests for identification of bacteria
  2. Preservation of stock cultures of bacteria

Suggested Readings

  1. Textbook of Microbiology- Ananthanarayan & Paniker (10th Ed)
  2. Medical Microbiology-by Fritz H. Kayser et al
  3. Medical Laboratory Technology by Kanai Lal Mukherjee, Publisher Tata McGrawHill
  4. Microbiology (7th Ed)- by Prescott
  5. Practical Book of Medical Microbiology by Satish Gupta, Publisher JaypeeBrothers
  6. Medical Laboratory Manual for Tropical Countries Vol. I and II by Monica Cheesbrough
  7. Essential Medical Microbiology- by Rajesh Bhatia (4th Ed)
  8. Clinical laboratory methods and diagnosis by Gradwohls, 2000, Publisher Mosby
  9. Medical laboratory science theory and practice, J Ochei and Kolhatkar, 2002, publisher TBS

Session Plan

Session 1

Session 2

Session 3

Session 4

Session 5

Session 6

Session 7

Session 8

Session 9

Session 10

Session 11

Session 12

Practice:

Preparation of bacterial smear and staining – Gram’s, Acid-fast, Staining of bacterial spores flagella, capsule, spirochaetes

Video 1: https://www.youtube.com/watch?v=c2BPE7wIK20

Video 2:https://www.youtube.com/watch?v=sxa46xKfIOY

Video 3:https://www.youtube.com/watch?v=NmfygqRQdeA

Video 4:https://www.youtube.com/watch?v=0yttbfBsSgk

Video 5:https://www.youtube.com/watch?v=mZGEhql02_I

Video 6: https://www.youtube.com/watch?v=cXYfT5hSLoQ

Practice:

Demonstration of various parts of microscope its functioning and care.

Video: https://www.youtube.com/watch?v=pujis3QsA_I

Session 13

Session 14

Session 15

Session 16

Session 17

Session 18

Session 19

chemical methods and their application

Video: https://www.youtube.com/watch?v=ujVE4R5vXtA

Presentation: https://pacificbiolabs.com/chemical-sterilization

Session 20

Session 21

Session 22

Session 23

Session 24

Practice:

Demonstration the different type of Sterilization technique and operation of the Instruments used in microbiological lab.

Video 1: https://www.youtube.com/watch?v=Dq5JgsxJpTY

Video 2: https://www.youtube.com/watch?v=bRadiLXkqoU

Video 3: https://www.youtube.com/watch?v=qpDkvectqGE

Practice :

Preparation of media, cultivation of bacteria

Video 1: https://www.youtube.com/watch?v=cneascR3OEc&t=14€7s

Video 2: https://www.youtube.com/watch?v=949qQtAnjIc

Session 25

Bacterial nutrition – Nutritional requirement of bacteria

Video: https://www.youtube.com/watch?v=jia7avWv4s4

Presentation: https://www.slideshare.net/RIZWANABBAS3/bacterial-nutrition-and-growth

Session 26

Bacterial nutrition – Nutritional requirement of bacteria

Video: https://www.youtube.com/watch?v=O9yRDmUxlp8

Presentation: https://www.slideshare.net/RIZWANABBAS3/bacterial-nutrition-and-growth

Session 27

Session 28

Session 29

Session 30

Session 31

Session 32

Session 33

Session 34

Session 35

Session 36

Sessions 37

Session 38

defined and synthetic media

Video: https://www.youtube.com/watch?v=_HDPGs4HLgQ

Session 39

Session 40

Session 41

Practice:

Biochemical tests for identification of bacteria

Video: https://www.youtube.com/watch?v=Eabt1kgy3ZQ

https://www.youtube.com/watch?v=fz_xksoQOuo&t=36s

Practice:

Preservation of stock cultures of bacteria

Video: https://www.youtube.com/watch?v=kXxmE5p-wF8&t=139s

Case Studies

Case Studies

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    Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]