per person /
Free
Genetics and Breeding
Teacher
Dr. Mangesh M Bhosale
Category
Core Courses
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Course Name : Genetics and Breeding
Code(Credit) : FSAQ 2111(1-1-0)
Course Objectives
At the end of the course, students will have knowledge on...
- Basics of genetics and its application in fisheries and aquaculture
- Role of population genetics in biodiversity assessment
- Fish breeding and sustainable production through genetic selection
Course Outcomes
| CO-1 | Demonstrate understanding on the fundamentals of genetics and fish genetics. PO 1, PO5, PO6 |
| CO-2 | Demonstrate the application of genetics in the field of aquaculture and species conservation. PO 1, PO 2, PO5 |
| CO-3 | Compare various hybridization techniques and methods of sex determination and manipulation. PO 1, PO7, PO9 |
PO1: Knowledge enhancement: Enhanced knowledge on concepts, essential facts, basics, principles and theories relating to fishery biology, biodiversity and aquatic environment, aquaculture, fish nutrition, fish physiology, genetics and biotechnology, fish processing, fisheries engineering, fish microbiology, fishery extension, economics, and biostatistics.
PO2: Professional and Entrepreneurial Skill development: Understanding the value and processes of life-long learning and professional enhancement and entrepreneurship development.
PO5: Environment and Sustainability: Understand the issues of environmental contexts and sustainable development.
PO6:Data Handling and computational skills: Collect, analyze and interpret biological and scientific data for the scientific and farming community with the help of computer-based programmes.
PO7:Communication & Extension Skills: Develop appropriate communication skills for effective transfer of knowledge and technologies through extension activities.
PO9: Ethics: Application of ethical principles and commitment to professional ethics and responsibilities.
Course Syllabus
Theory
Unit I: Principles of genetics and breeding, Gene and chromosome as basis of inheritance, Mendel’s law of inheritance – complete and incomplete dominance, monohybrid and dihybrid ratios.
Unit II: Gene interactions – dominant and recessive epistasis. Pleiotropism. Lethal genes. Mutation.
Unit III: Sex - linked genes, sex influenced and sex limited traits. Linkage and crossing over.
Unit IV: Introduction to population genetics. Hardy- Weinberg law and its significance.
Unit V: Chromosomal structure and aberrations. Chromosome manipulation techniques - androgenesis, gynogenesis and polyploidy and identification of ploidy. Sex determination.
Unit VI: Cross breeding (hybridization)– types of cross breeding, heterosis and design of cross breeding programmes, hybridization in different fishes. Quantitative genetics – quantitative traits, polygenic traits, heritability.
Unit VII: History and present status of selective breeding programs in aquaculture. Selection methods and mating designs. Design for selective breeding. Inbreeding and its consequences. Domestication methods.
Unit VIII: Seed certification and quarantine procedures. Cryopreservation of gametes.
Practicals
- Problems on Mendelian inheritance (qualitative genetics) - monohybrid and dihybrid ratios and epistasis.
- Problems on quantitative traits, response to selection and heritability.
- Estimation of rate of inbreeding and heterosis.
- Mitotic and meiotic chromosome preparation.
- Demonstration of protocol of androgenesis, gynogenesis and polyploidy.
- Problems on gene and genotypic frequency.
- Gamete cryopreservation protocols and quality evaluation of fish milt.
Reference Books
- Klug, W.S., Cummings, M.R., Spencer, C.A and Palladino, M.A 2019. Concept of genetics. Pearson Education India
- Padhi, B.K and Mandal, R.K. 2000. Applied Fish Genetic. Fishing Chimes
- Lakra, W.S. 2008. Fish Genetics & Biotechnology. Narendra Publishing House, New Delhi.
- Primrose, S.B. and Twyman, R.M. 2006. Principles of Gene Manipulation. Blackwell Publishing, Malden, USA
- Dunham, R. A. 2004. Aquaculture and Fisheries Biotechnology : Genetic Approaches. CABI Publishing
- Thomas, P.C., Rath, S.C. and Mohapatra, K.D. 2003. Breeding and Seed Production of Finfish and Shellfish. Astral Publishing House
Session Plan
Session 1
Introduction to genetics
https://www.youtube.com/watch?v=ij2azDtSED0
Session 2
Principles of genetics and breeding
https://www.msdmanuals.com/home/fundamentals/genetics/genes-and-chromosomes
Session 3
Gene and chromosome as basis of inheritance
Session 11
Session 15
Session 16
Quantitative genetics
quantitative traits
polygenic traits
heritability
Practicals
Session 5-6
Problems on response to selection and heritability
Session 7
Counting of fish sperm and checking the viability
Session 8
Protocol for production of androgenic progenies
Session 9-10
Protocol for production of gynogenesis progenies
Session 11-12
Protocol for production of Polyploidy progenies
Session 13-14
Preparation of Metaphase Chromosomes for Cytogenetic Analysis
Session 15
Problems on gene and genotypic frequency
Session 16
Protocol for Mitotic and meiotic chromosome preparation
Our Main Teachers
Specialization in Aquaculture Fish Genetics and Breeding, Fish Biotechnology and Bioinformatics, Fish reproduction, Aquariculture, Advanced Aquaculture Systems, (Recirculatory Aquaculture Systems, Biofloc, Raceways etc.), Geometric and Truss Morphometry, DNA Barcoding, Live food production techniques