Hematology
Teacher
Sonalika Mahapatra
Category
Core Courses
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Course Name : Hematology
Code(Credit) : CUTM1731 (3-2-0)
Course Objectives
- The overall aims are that the student should obtain advanced knowledge of the most common hematologic diseases & understanding the concept of Blood cells and other blood components.
- Be able to handle an investigation of hemorrhagic disorder and laboratory abnormalities such as anaemia, polycythemia, leukopenia, leukocytosis, thrombocytopenia, thrombocytosis, elevated ESR etc within hematology.
- Clinically relevant hematological analysis for deeper understanding of Evaluate normal and abnormal cell morphology with associated diseases and other blood components .
Course Outcomes
COs |
Course Outcomes |
Mapping COs with POs (High-3, Medium-2, Low- 1) |
CO1 |
Understand the scope and importance of Haematology and its laboratory tests. (Knowledge) |
PO1(3), PO8(3) |
CO2 |
Identify and operate important equipment and chemicals used in haematology laboratory. (Comprehension) |
PO2(3), PO8(3), PO9(2) |
CO3 |
Demonstrate the procedure for focusing different blood cells through a microscope. (Application) |
PO2(3) PO5(2),,PO(11) |
Course Syllabus
Module- I ( 8 Hrs)
Scope & importance of Hematology, important equipment and chemicals, various test performed in Hematology laboratory,Focusing different blood cells through microscope.
Practice: Demonstration of instruments used in hematology- Microscope, Blood Cell counter, Sahali’s Apparatus,
Module- II ( 12 Hrs)
Identify and/or confirm the composition & function of various red blood cell inclusions.Function of normal cellular components. Formation of blood, Synthesis of blood in Bone marrow- Erythropoiesis, leucopoiesis, thrombopoiesis. Anticoagulants: definition, Uses, Different types of Anticoagulants., mode of action, their merits and demerits.
Morphology of normal blood cells, abnormal morphology & diseases.
Practice: Demonstration of different blood cell, their synthesis from slide presentation or chart.
Demonstration the normal and abnormal morphology of different blood cells.
Module- III (10 Hrs)
Hematological Disorders
- Classification of Anemia: Morphological &etiological.
- Iron Deficiency Anemia: Distribution of body Iron, Iron Absorption,causes of iron deficiency, lab findings.
- Megaloblastic Anemia: Causes, Lab findings.
- Hemolytic Anemia: Definition, causes, classification & lab findings.
Bone Marrow: Cell composition of normal adult Bone marrow, Aspiration,Indication,
Preparation & Staining, Special Stain for Bone Marrow -Periodic Acid Schiff, Sudan Black
Leukemia: Classification, Blood Picture, Differentiation of BlastCells.
Practice: Collection of blood by different methods
Different normal and abnormal morphology of RBCs, WBCs, Platelet.
Module- IV (10 Hrs)
Collection of blood, Methods & Preparation of Stains and Smears
Practice:
leaning and drying of glass and plastic ware, Collection of venous and capillary blood, cleaning of glass-syringes and its sterilization. Preparation of buffers, Preparation of the stains and other reagents, Preparation of peripheral blood film (PBF), To stain a peripheral blood Film by Leishman- stain, Haemoglobin estimation (Sahali's method and cyanmethaemoglobin method).
Module- V (10 Hrs)
Routine Hematological Tests:
Complete blood cell count, ESR, Differential Leukocyte count, Total leukocyte count, Bleeding time and Clotting time, Blood Grouping and Rh Typing.
Practice:
Complete Blood Counts, Determination of Haemoglobin, TRBC Count by Haemocytometers, TLC by Haemocytometer, Differential Leukocyte count, Determination of Platelet Count. Determination of ESR by wintrobes, Determination of ESR by Westergeren's method, Determination of PCV by Wintrobes, Erythrocyte Indices- MCV, MCH, MCHC. Reticulocyte Count, Absolute Eosinophil Count, Bleeding time and Clotting time, Blood Grouping and Rh Typing
Suggested Readings:
- Textbook of Medical Laboratory Technology P.B Gotkar Mumbai, Bhalani Publishing House
- Text book of Medical Laboratory Technology by Paraful B. Godkar, Publisher Bhalani
- Text book of Medical Laboratory Technology (2nd edition) by V.H Talib, Publisher CBS
- Atlas of hematology (5th edition) by G.A. McDonald, Publisher Churchill Livingstone
- Medical Laboratory Technology By K.L Mukharjee, Publisher McGraw Hill education pvtlimited
- Text book of Medical Laboratory Technology (6th edition) by Ramnik Sood,Jaypee Publication.
- Ebook link-https://www.pdfdrive.com/hematology-basic-principles-and-practice-e176384006.html
- Ebook link-https://www.pdfdrive.com/hematology-basic-principles-and-practice-expert-consult-online-and-print-expert-consult-title-online-print-5th-edition-e186195241.html
- Ebook link-https://books.google.co.in/books?id=6sfacydDNsUC&printsec=frontcover&dq=hematology&hl=en&sa=X&ved=2ahUKEwja9-ve3I7qAhUwzTgGHSMUDekQ6wEwAHoECAQQAQ#v=onepage&q=hematology&f=false
- Ebook link-https://books.google.co.in/books?id=QQcYAAAAYAAJ&printsec=frontcover&dq=hematology&hl=en&sa=X&ved=2ahUKEwja9-ve3I7qAhUwzTgGHSMUDekQ6wEwAnoECAIQAQ#v=onepage&q=hematology&f=false
Simulation links for labs:
All the practice components can be performed by simulation as well as virtual labs. Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg), labsforlife (http://labsforlife.in/)
Online Source: Udemy, Coursera, article library, NCBI,Study.com, You tube, Slide share, Frontier, Febs, unacademy, academia,
Session Plan
Session 1
Topic: Scope of Hematology
Study Materials:
Description: https://spark.adobe.com/page/EHd5QQBEx23S9/
Video- https://www.youtube.com/watch?v=3VlckXrY9TU
https://www.youtube.com/watch?v=FcGZH93xubs
http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Hematology-1.webm
Session 2
Topic: important equipment and chemicals
Study Materials:
Discussion-https://spark.adobe.com/page/mBMmZGYEcIIXO/
Notes: https://spark.adobe.com/page/9VyTkbabnWN8u/
Video: http://courseware.cutm.ac.in/wp-content/uploads/2020/09/Basic-Lab-Equipment-1.mp4
Session 3
Topic: A various test performed in the Hematology laboratory
Study Materials:
Discussion-https://spark.adobe.com/page/bf83XWFMN5kQa/
https://study.com/academy/lesson/what-is-a-hematology-test-common-tests-interpretations.html
Slide share- https://www.slideshare.net/hillainemarie/hematology-laboratory-tests
Notes- https://www.hopkinsmedicine.org/health/treatment-tests-and-therapies/hematology
Session 4
Practice Session:
Topic: Focusing Slide under Microscope
Study Materials:
Video- https://www.youtube.com/watch?v=SUo2fHZaZCU&t=103s
https://www.youtube.com/watch?v=27EUjnymG5c
Topic: different blood cells through microscope.
Session 5
Topic: Composition and function of Red blood cell
Study Materials:
Video- https://www.youtube.com/watch?v=VSVYgivfs9c
https://www.youtube.com/watch?v=qrE6Y0Se8bw&pbjreload=101
Slide share- https://www.slideshare.net/BijiSaro/blood-composition-and-its-functions-on-17092016
Session 6
Topic: Function of normal cellular components
Study Materials:
Session 7
Session 8
Topic: Synthesis of blood in Bone marrow
Study Materials:
Video- https://www.youtube.com/watch?v=vyhYK50i8yQ
Discussion- https://www.leukaemia.org.au/disease-information/blood-basics/bone-marrow-blood-formation/
Session 9
Topic: Erythropoiesis
Study Materials:
Video- https://www.youtube.com/watch?v=YEt2D8xM9b0
https://www.youtube.com/watch?v=aE4d7JS-TTQ
https://www.coursera.org/lecture/immunologyfundamentalsimmunitybcells/hematopoiesis-ooWGI
Session 11
Session 12
Topic: Anticoagulants: definition, Types & Uses
Study Materials:
Session 13
Topic: Anticoagulants: mode of action
Study Materials:
Video- https://www.youtube.com/watch?v=eZBtQ0rDnG4
https://www.slideshare.net/peddanasunilkumar/anticoagulants-used-in-haematology
Session 14
Topic: Anticoagulants: merits and demerits
Study Materials:
Slide share- https://www.slideshare.net/peddanasunilkumar/anticoagulants-used-in-haematology
Session 15
Topic: Morphology of normal Red blood cells and abnormal morphology with diseases
Study Materials:
Video- https://www.youtube.com/watch?v=6PITMN4lil4
Session 16
Topic: Hematological Disorders
Study Materials:
Video- https://www.youtube.com/watch?v=06gtqiIWd0I
Session 17
Topic: Classification of Anemia: Morphological & etiological
Study Materials:
Session 18
Topic: Distribution & Absorption of Iron in body
Study Materials:
Video- https://www.youtube.com/watch?v=y1HGudIjkVg
Slide share- https://www.slideshare.net/nikhilgupta129/iron-physiology
Session 19
Topic: Iron Deficiency Anemia: Causes, Symptoms & Lab Diagnosis
Study Materials:
Video- https://www.youtube.com/watch?v=WvD4p8FkQpY&t=462s
https://www.youtube.com/watch?v=U1Ls9Vh-3m8
https://www.youtube.com/watch?v=WvD4p8FkQpY&t=79s
Slide share- https://www.slideshare.net/VarugheseGeorge/laboratory-diagnosis-of-anemia
Discussion- https://www.healthline.com/health/iron-deficiency-anemia
Session 20
Practice Session:
Topic: Collection of venous blood
Study Materials:
Session 21
Practice Session:
Topic: Collection of capillary blood
Study Materials:
Session 22
Topic: Cleaning and drying of glass and plastic ware
Study Materials:
Session 23
Topic: Cleaning of glass-syringes and its sterilization
Study Materials:
Session 24
Session 25
Topic: Preparation of the stains and other reagents
Study Materials:
Slide share- https://www.slideshare.net/DrSHAHIDRaza/special-and-routine-stains-in-haematology-1
Session 26
Practice Session:
Topic: Preparation of peripheral blood film (PBF)
Study Materials:
Video- https://www.youtube.com/watch?v=cI9GObT73lY
https://www.youtube.com/watch?v=ihSnTKLUazQ
Session 27
Practice Session:
Topic: Maleria Thick smear preparation
Study Materials:
Session 28
Practice Session:
Topic: To stain a peripheral blood Film by Leishman- stain
Study Materials:
Session 29
Practice Session:
Topic: Haemoglobin estimation (Sahali's method)
Study Materials:
Session 30
Practice Session:
Topic: Haemoglobin estimation (cyanmethaemoglobin method)
Study Materials:
Session 31
Practice Session:
Topic: Complete Blood Counts
Study Materials:
Video- https://www.youtube.com/watch?v=j7PJrUFciec
https://www.youtube.com/watch?v=20x2AQNZUfQ
https://www.youtube.com/watch?v=7kqpOz5VfU0
CBC Notes: https://spark.adobe.com/page/RTysjE2z4YxVJ/
Video: http://courseware.cutm.ac.in/wp-content/uploads/2020/09/CBC.mp4
Session 32
Practice Session:
Topic: Hemocytometer calculation Method
Study Materials:
Session 33
Practice Session:
Topic: TRBC Count by Haemocytometers
Study Materials:
Video- https://www.youtube.com/watch?v=JzGW9PovzGg
Session 34
Practice Session:
Topic: TLC by Haemocytometer
Study Materials:
Session 35
Practice Session:
Topic: Differential Leukocyte count
Study Materials:
Session 36
Practice Session:
Topic: Determination of Platelet Count
Study Materials:
Session 37
Practice Session:
Topic: Determination of ESR by wintrobes
Study Materials:
ESR Notes: https://spark.adobe.com/page/acSDqWUYI1Pbt/
Video: http://courseware.cutm.ac.in/wp-content/uploads/2020/09/ESR.mp4
Session 38
Practice Session:
Topic: Determination of ESR by Westergeren's method
Study Materials:
ESR Notes: https://spark.adobe.com/page/acSDqWUYI1Pbt/
Video: http://courseware.cutm.ac.in/wp-content/uploads/2020/09/ESR.mp4
Session 39
Practice Session:
Topic: Determination of PCV by Wintrobes
Study Materials:
Session 40
Topic: Erythrocyte Indices- MCV, MCH, MCHC
Study Materials:
Video- https://www.youtube.com/watch?v=Uz1zSNZI2H0
Session 41
Practice Session:
Topic: Reticulocyte Count
Study Materials:
Session 42
Practice Session:
Topic: Absolute Eosinophil Count
Study Materials:
Session 43
Practice Session:
Topic: Bleeding time & clotting time
Study Materials:
Video- https://www.youtube.com/watch?v=bMVy6pCWhRk
https://www.youtube.com/watch?v=AYipSViRduE
https://www.youtube.com/watch?v=CG3_u9oCl7Q
https://www.youtube.com/watch?v=ejt9FjlJJ8c
BT- CT Notes: https://spark.adobe.com/page/N64Fni9BLOsqA/
BT-CT Video: http://courseware.cutm.ac.in/wp-content/uploads/2020/09/BT-CT.mp4
Session 44
Practice Session:
Topic: Blood Grouping and Rh typing
Study Materials:
Blood grouping Notes: https://spark.adobe.com/page/LELbPicU9N1yH/
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