Susmita Chakrabarty


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Course Name : Hematology

Code(Credit) : CUTM1731 (3-2-0)

Course Objectives

  • The overall aims are that the student should obtain advanced knowledge of the most common hematologic diseases & understanding the concept of Blood cells and other blood components.
  • Be able to handle an investigation of hemorrhagic disorder and laboratory abnormalities such as anaemia, polycythemia, leukopenia, leukocytosis, thrombocytopenia, thrombocytosis, elevated ESR etc within hematology.
  • Clinically relevant hematological analysis for deeper understanding of Evaluate normal and abnormal cell morphology with associated diseases and other blood components .

Learning Outcomes

  • Differentiate various laboratory test findings with their associated clinical conditions.
  • Identify the various skills necessary to perform blood counts, evaluate blood elements,and report results within the stated limits of accuracy.
  • Describe the various components of blood ,their functions,and roles in various disease states.
  • To be able to demonstrate good skills in the relevant Hematology laboratory methodology.
  • Collection of blood for the investigations.
  • Be able to distinguish the developmental stages of blood cells.It will also cover Bone marrow examination.
  • To learn about tests carried out for hematological investigations.
  • To be able to carry out blood sampling.

Course Syllabus

Module- I ( 8 Hrs)


Scope & importance of  Hematology, important equipment and chemicals, various test performed in Hematology laboratory,Focusing different blood cells through microscope.


Practice: Demonstration of instruments used in hematology- Microscope, Blood Cell counter, Sahali’s Apparatus,


Module- II ( 12 Hrs)


Identify and/or confirm the composition & function of various red blood cell inclusions.Function of normal cellular components. Formation of blood, Synthesis of blood in Bone marrow- Erythropoiesis, leucopoiesis, thrombopoiesis. Anticoagulants: definition, Uses, Different types of Anticoagulants., mode of action, their merits and demerits.

Morphology of normal blood cells, abnormal morphology & diseases.


Practice: Demonstration of different blood cell, their synthesis from slide presentation or chart.

Demonstration the normal and abnormal morphology of different blood cells. 


Module- III (10 Hrs)


Hematological Disorders

  1. Classification of Anemia: Morphological &etiological.
  2. Iron Deficiency Anemia: Distribution of body Iron, Iron Absorption,causes of iron deficiency, lab findings.
  3. Megaloblastic Anemia: Causes, Lab findings.
  4. Hemolytic Anemia: Definition, causes, classification & lab findings.


Bone Marrow: Cell composition of normal adult Bone marrow, Aspiration,Indication,

Preparation & Staining, Special Stain for Bone Marrow -Periodic Acid Schiff, Sudan Black

Leukemia: Classification, Blood Picture, Differentiation of BlastCells.


Practice: Collection of blood by different methods

Different normal and abnormal morphology of RBCs, WBCs, Platelet.


Module- IV (10 Hrs)

Collection of blood, Methods & Preparation of Stains and Smears



leaning and drying of glass and plastic ware, Collection of venous and capillary blood, cleaning of glass-syringes and its sterilization. Preparation of buffers, Preparation of the stains and other reagents, Preparation of peripheral blood film (PBF), To stain a peripheral blood Film by Leishman- stain, Haemoglobin estimation (Sahali's method and cyanmethaemoglobin method).


Module- V (10 Hrs)

Routine Hematological Tests:

Complete blood cell count, ESR, Differential Leukocyte count, Total leukocyte count, Bleeding time and Clotting time, Blood Grouping and Rh Typing.



Complete Blood Counts, Determination of Haemoglobin, TRBC Count by Haemocytometers, TLC by Haemocytometer, Differential Leukocyte count, Determination of Platelet Count. Determination of ESR by wintrobes, Determination of ESR by Westergeren's method, Determination of PCV by Wintrobes, Erythrocyte Indices- MCV, MCH, MCHC. Reticulocyte Count, Absolute Eosinophil Count, Bleeding time and Clotting time, Blood Grouping and Rh Typing


Suggested Readings:

  1. Textbook of Medical Laboratory Technology P.B Gotkar Mumbai, Bhalani Publishing House
  2. Text book of Medical Laboratory Technology by Paraful B. Godkar, Publisher Bhalani
  3. Text book of Medical Laboratory Technology (2nd edition) by V.H Talib, Publisher CBS
  4. Atlas of hematology (5th edition) by G.A. McDonald, Publisher Churchill Livingstone
  5. Medical Laboratory Technology By K.L Mukharjee, Publisher McGraw Hill education pvtlimited
  6. Text book of Medical Laboratory Technology (6th edition) by Ramnik Sood,Jaypee Publication.
  7. Ebook link-https://www.pdfdrive.com/hematology-basic-principles-and-practice-e176384006.html
  8. Ebook link-https://www.pdfdrive.com/hematology-basic-principles-and-practice-expert-consult-online-and-print-expert-consult-title-online-print-5th-edition-e186195241.html
  9. Ebook link-https://books.google.co.in/books?id=6sfacydDNsUC&printsec=frontcover&dq=hematology&hl=en&sa=X&ved=2ahUKEwja9-ve3I7qAhUwzTgGHSMUDekQ6wEwAHoECAQQAQ#v=onepage&q=hematology&f=false
  10. Ebook link-https://books.google.co.in/books?id=QQcYAAAAYAAJ&printsec=frontcover&dq=hematology&hl=en&sa=X&ved=2ahUKEwja9-ve3I7qAhUwzTgGHSMUDekQ6wEwAnoECAIQAQ#v=onepage&q=hematology&f=false



Simulation links for labs: 

All the practice components can be performed by simulation as well as virtual labs. Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg), labsforlife (http://labsforlife.in/)

Online Source: Udemy, Coursera, article library, NCBI,Study.com, You tube, Slide share, Frontier, Febs, unacademy, academia,

Session 3

Topic: A various test performed in the Hematology laboratory

Study Materials:



Slide share- https://www.slideshare.net/hillainemarie/hematology-laboratory-tests

Notes- https://www.hopkinsmedicine.org/health/treatment-tests-and-therapies/hematology

Session 4

Practice Session:

Topic: Focusing Slide under Microscope

Study Materials:

Video- https://www.youtube.com/watch?v=SUo2fHZaZCU&t=103s


Topic: different blood cells through microscope.

Video- https://www.youtube.com/watch?v=_Hs-kUBbakU


Session 8

Session 10

Session 11

Session 12

Topic: Anticoagulants: definition, Types & Uses

Study Materials:

Video- https://www.youtube.com/watch?v=jrWD83b8hkA


Session 14

Topic: Anticoagulants: merits and demerits

Study Materials:

Slide share- https://www.slideshare.net/peddanasunilkumar/anticoagulants-used-in-haematology

Session 15

Topic: Morphology of normal Red blood cells and abnormal morphology with diseases

Study Materials:

Video- https://www.youtube.com/watch?v=6PITMN4lil4



Session 17

Topic: Classification of Anemia: Morphological & etiological

Study Materials:

Video- https://www.youtube.com/watch?v=t6Kh0dSWamo


Session 18

Topic: Distribution & Absorption of  Iron in body

Study Materials:

Video- https://www.youtube.com/watch?v=y1HGudIjkVg

Slide share- https://www.slideshare.net/nikhilgupta129/iron-physiology

Session 20

Practice Session:

Topic: Collection of venous blood

Study Materials:

Video- https://www.youtube.com/watch?v=r8n2O1GIqQg


Session 21

Practice Session:

Topic: Collection of capillary blood

Study Materials:

Video- https://www.youtube.com/watch?v=8-NVVSOBUu8


Session 22

Topic: Cleaning and drying of glass and plastic ware

Study Materials:

Video- https://www.youtube.com/watch?v=W0oacysFTko

Session 23

Topic: Cleaning of glass-syringes and its sterilization

Study Materials:

Video- https://www.youtube.com/watch?v=9pfjcsIeG74

Session 24

Topic: Preparation of buffers

Study Materials:

Video- https://www.youtube.com/watch?v=kdhmryQACz8

Session 25

Topic: Preparation of the stains and other reagents

Study Materials:

Slide share- https://www.slideshare.net/DrSHAHIDRaza/special-and-routine-stains-in-haematology-1

Session 27

Practice Session:

Topic: Maleria Thick smear preparation

Study Materials:

Video- https://www.youtube.com/watch?v=WPP7AjmStBg

Session 28

Practice Session:

Topic: To stain a peripheral blood Film by Leishman- stain

Study Materials:

Video- https://www.youtube.com/watch?v=Aa0XvwGXsK8

Session 29

Practice Session:

Topic: Haemoglobin estimation (Sahali's method)

Study Materials:

Video- https://www.youtube.com/watch?v=qMTJ05l855Q


Session 30

Practice Session:

Topic: Haemoglobin estimation (cyanmethaemoglobin method)

Study Materials:

Video- https://www.youtube.com/watch?v=deydnUQdhzA&t=66s


Session 32

Practice Session:

Topic: Hemocytometer calculation Method

Study Materials:

Video- https://www.youtube.com/watch?v=wF-VVyisbGw&t=106s


Session 33

Session 34

Practice Session:

Topic: TLC by Haemocytometer

Study Materials:

Video- https://www.youtube.com/watch?v=MKS0KM3lr90


Session 35

Practice Session:

Topic: Differential Leukocyte count

Study Materials:

Video- https://www.youtube.com/watch?v=u-pykuHqQmU


Session 36

Practice Session:

Topic: Determination of Platelet Count

Study Materials:

Video- https://www.youtube.com/watch?v=Qf5nXANq6o0


Session 39

Practice Session:

Topic: Determination of PCV by Wintrobes

Study Materials:

Video- https://www.youtube.com/watch?v=DfG6C0gKjhU


Session 41

Practice Session:

Topic: Reticulocyte Count

Study Materials:

Video- https://www.youtube.com/watch?v=5ipo6IPhKR8


Session 42

Practice  Session:

Topic: Absolute Eosinophil Count

Study Materials: 

Video- https://www.youtube.com/watch?v=g5x2rflR1WM


Session 44

Practice Session:

Topic: Blood Grouping and Rh typing

Study Materials:

Blood grouping Notes: https://spark.adobe.com/page/LELbPicU9N1yH/

Video- https://www.youtube.com/watch?v=cKnEdvrmHK4


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    Susmita Chakrabarty is currently working as an Assistant Professor in the School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes the field of Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique, Analytical Biochemistry & Clinical Biochemistry, etc. […]