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Module- I ( 8 Hrs)
Scope & importance of Hematology, important equipment and chemicals, various test performed in Hematology laboratory,Focusing different blood cells through microscope.
Practice: Demonstration of instruments used in hematology- Microscope, Blood Cell counter, Sahali’s Apparatus,
Identify and/or confirm the composition & function of various red blood cell inclusions.Function of normal cellular components. Formation of blood, Synthesis of blood in Bone marrow- Erythropoiesis, leucopoiesis, thrombopoiesis. Anticoagulants: definition, Uses, Different types of Anticoagulants., mode of action, their merits and demerits.
Morphology of normal blood cells, abnormal morphology & diseases.
Practice: Demonstration of different blood cell, their synthesis from slide presentation or chart.
Demonstration the normal and abnormal morphology of different blood cells.
Module- III (10 Hrs)
Bone Marrow: Cell composition of normal adult Bone marrow, Aspiration,Indication,
Preparation & Staining, Special Stain for Bone Marrow -Periodic Acid Schiff, Sudan Black
Leukemia: Classification, Blood Picture, Differentiation of BlastCells.
Practice: Collection of blood by different methods
Different normal and abnormal morphology of RBCs, WBCs, Platelet.
Module- IV (10 Hrs)
Collection of blood, Methods & Preparation of Stains and Smears
leaning and drying of glass and plastic ware, Collection of venous and capillary blood, cleaning of glass-syringes and its sterilization. Preparation of buffers, Preparation of the stains and other reagents, Preparation of peripheral blood film (PBF), To stain a peripheral blood Film by Leishman- stain, Haemoglobin estimation (Sahali's method and cyanmethaemoglobin method).
Module- V (10 Hrs)
Routine Hematological Tests:
Complete blood cell count, ESR, Differential Leukocyte count, Total leukocyte count, Bleeding time and Clotting time, Blood Grouping and Rh Typing.
Complete Blood Counts, Determination of Haemoglobin, TRBC Count by Haemocytometers, TLC by Haemocytometer, Differential Leukocyte count, Determination of Platelet Count. Determination of ESR by wintrobes, Determination of ESR by Westergeren's method, Determination of PCV by Wintrobes, Erythrocyte Indices- MCV, MCH, MCHC. Reticulocyte Count, Absolute Eosinophil Count, Bleeding time and Clotting time, Blood Grouping and Rh Typing
Simulation links for labs:
All the practice components can be performed by simulation as well as virtual labs. Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg), labsforlife (http://labsforlife.in/)
Online Source: Udemy, Coursera, article library, NCBI,Study.com, You tube, Slide share, Frontier, Febs, unacademy, academia,
Topic: Scope of Hematology
Topic: important equipment and chemicals
Topic: various test performed in Hematology laboratory
Topic: Focusing Slide under Microscope
Topic: different blood cells through microscope.
Topic: Composition and function of Red blood cell
Topic: Function of normal cellular components
Topic: Formation of blood
Topic: Synthesis of blood in Bone marrow
Topic: Morphology of normal Red blood cells and abnormal morphology with diseases
Topic: Hematological Disorders
Topic: Iron Deficiency Anemia: Causes, Symptoms & Lab Diagnosis
Topic: Preparation of peripheral blood film (PBF)
Topic: Erythrocyte Indices- MCV, MCH, MCHC
Miss Susmita Chakrabarty is currently working as a Lecturer in School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes field in Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique & Clinical Biochemistry etc. In her research, […]