Immunology
Teacher
Dr Sitaram Swain
Category
Core Courses
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Course Name : Immunology
Code(6) :CUTM1705(3-1-2)
Course Objectives
- The primary objective of this course is to help students develop knowledge and skills related to health and disease and role of immune system.students are taught immunology so as to develop understanding of the subject, such as functioning the immune system, the molecular and cellular components and pathways that protect an organism from infectious agents
Learning Outcomes
- •Understand the immunomodulatory strategies essential for generating or suppressing immune responses as required in hypersensitivity reactions, transplantation, autoimmune diseases and cancer.
• Students will know the principle and application of various immune techniques and they can Will be able to make a strategy for immunological research and execute it.
Course Syllabus
Module:I Immunity: Historical Perspective, Cellular and humoral immunity. Role of immunologically important organs and cells, bone marrow, thymus, spleen and lymphocytes. Formation and functions of T and B Lymphocytes. Helper T-cells and killer T-cells. Macrophages.
Module:II Complement activation by classical, alternate and MB lectin pathway, biological consequences of complement activation, regulation and complementdeficiencies.
Module: III Antigens: Definition, epitopes, haptens, carriers, adjuvants, antigenicity. Antibodies: Definition, types and structure of immunoglobulins (IgG – light chain, heavy chain, hyper variable region, constant domains, Fab and Fc). Effector functions of antibody, antigenic determinants on Ig, Ig super family. Monoclonal antibodies production and applications.
Module: IV Major histocompatibility complex (MHC) genes and products – Polymorphism of MHC genes, role of MHC antigens in immune responses, MHC antigens in transplantation.
Module V: Antigen–antibody reaction in vitro: Formation of antigen-antibody complex. Application of immunodiffusion, RIA, ELISA (Basic principles only).
Module:VI Immunization: Vaccination – vaccines and their preparations, primary and secondary immune response, active and passive immunization, types of vaccines, immunosuppressive therapy.
Module:VII Autoimmunity and hypersensitivity: Self-tolerance and possible mechanisms of induction of autoimmunity, Organ specific and systemic autoimmune diseases, Types of hypersensitivity.Immunological disorders: Allergy and AIDS. Structure of HIV, mode of transmission.
Practical content
1. Isolation of lymphocytes from blood / spleen.
2. Ouchterlony double immunodiffusion (DID)
3. Purification ofimmunoglobulins
4. Demonstration of Western Blotting
5. Assays based on agglutination reactions - Blood typing (active) and passive agglutination.
6. Enzyme linked immunosorbent assay (ELISA) & DOTELISA
Suggested Readings:
• I.M. Riott, J. Brostoff, D. Male “Immunology” 3rd edn. W.H. Freeman and Pub. Company, USA.
• J. Kuby “Immunology” 3rd edn., Mosby Year Book Co., England
• Introduction to Immunology – NandiniShetty (2003)
• Immunology – Janis Kuby – W. H. Freeman and Co. 7th edition (2019)
• Janeway’sImmunobiology 2012 8th ed., Murphy, K., Mowat, A., and Weaver, C.T.,
• Garland Science (London & New York), ISBN:978-0-8153-4243-4
Session 13
biological consequences of complement activation.
Session 14
Session 16
Practice: Ouchterlony double immunodiffusion (DID)
Session 20
Antibodies: Definition, types and structure of immunoglobulins
Session 21
Flipped class: Antibodies: structure of immunoglobulins
Session 23
IgG – light chain, heavy chain, hyper variable region, constant domains, Fab and Fc
Session 28
Monoclonal antibodies production and applications.
Session 29
Flipped class:Production of Monoclonal antibodies production.
Session 30
Practice:Purification of immunoglobulins
Session 39
flipped class: Different antigen-antibody reaction
Session 44
Immunization: Vaccination – vaccines and their preparations
Session 46
primary and secondary immune response,active and passive immunization
Session 52
Organ specific and systemic autoimmune diseases, Types of hypersensitivity.Immunological disorders: Allergy and AIDS
Session 49
Autoimmunity and hypersensitivity:Self-tolerance and possible mechanisms of induction of autoimmunity
Case Studies
Case Studies
Our Main Teachers
Dr Sitaram Swain has received PhD degree from Utkal University in the year 2017. He is also trained and experienced from TATA Memorial Hospital, Mumbai, India. He has experienced with cell line culture, clinical biochemistry, immuno-fixation, immunoassay, cancer cytogenetic, serum protein electrophoresis and tumour marker analysis. He has qualified NET(ICAR-ASRB) in Animal biotechnology. He has […]