Immunology and Parasitology

Teacher

Dr. Soumya Jal

Category

Core Courses

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Course Name : Immunology and Parasitology

Code(Credit) : CUTM1728 (3-2-0)

Course Objectives

  • Understanding the concept of Innate & adaptive immune system; complement system; Hypersensitivity.
  • Clinically relevant serological analysis for deeper understanding of antigen-antibody interaction.
  • To understand the concept of cells of immune system and organs of immune system.
  • To understand the characteristics of parasites and their examination.

Learning Outcomes

  • The student will learn the application of Immunology in disease diagnosis.
  • Complement system followed by the body on encountering an Antigen.
  • Immune Response produced on encounter with foreign body.
  • The students will learn the role of immunity in fighting disease, along with consequence of undesirable expression of immune system such as, hypersensitivity and auto immune disease.
  • They will able to learn the techniques of isolating parasites.
  • They will also be able to diagnose the parasites.

Course Syllabus

Module– 1

Immunity: Concept of Innate and Adaptive immunity.

Immune Cells and Organs: Structure, Functions and Properties of: Immune Cells – Stem cell, T cell, B cell, NK cell, Macrophage, Neutrophil, Eosinophil, Basophil, Mast cell, Dendritic cell; and Immune Organs – Bone Marrow, Thymus, Lymph Node, Spleen, GALT, MALT, CALT

Antigens: Characteristics, Hapten, Epitopes, Adjuvants;

Antibody: Structure & its classes.

Antigen-Antibody interaction, avidity & affinity.

Serological Reactions: Principles of Precipitation, Agglutination, Immunodiffusion, Immunoelectrophoresis, ELISA, ELISPOT, Western blotting, Immunofluoresence, Immunoelectron microscopy.

Practice:

Performing Serological tests:

Widal test

VDRL test

ASO test

C-Reactive Protein test

Rheumatoid factor (RF) test

Precipitation in agarose gel

Performing Ouchterlony Double diffusion test

Demonstration of SDS-PAGE

Demonstration of ELISA

Demonstration of Western blotting

 

Module – 2

Complement System: Role of complement system in immune response, Complement components and Activation pathways.

Immune Response: Cell mediated and humoral Immunity.

Monoclonal antibodies: Production, characterization and applications.

Types of Autoimmunity and Hypersensitivity with examples; Immunodeficiencies, SCID, DiGeorge syndrome, Chediak- Higashi syndrome, Leukocyte adhesion deficiency, CGD

 

Module-3

Medical parasitology: Introduction to medical Parasitology, Morphology, General   Characteristics, Classification, Laboratorial diagnosis of different human pathogenic parasites. Examination of faeces for ova and cysts, Concentration methods. Blood smear examination for Parasites. Cultivation of Protozoan Parasites.

Practice:

Concentration techniques for demonstration of Ova

Routine Stool examination for detection of intestinal parasites

Identification of adult worms from models or slide’s

Identification of different parasites, their morphology from slide’s

 

Suggested Readings:

  1. Kuby's Immunology (7th Ed) - by J. Owen, J. Punt, S. Strandford. Macmillan Higher Education, England.

(e-book link: https://www.pdfdrive.com/kuby-immunology-7th-edition-2013-e44842271.html )

  1. Roitt's Essential Immunology (13th Ed)- by Seamus J. Martin, Dennis R. Burton, Ivan M. Roitt. Wiley Blackwell.

(e-book link: http://dl.mehrsys.ir/pdf-books/Roitt_s%20Essential%20Immunology%20Thirteenth%20Edition(www.myuptodate.com).pdf )

  1. Prescott, Harley, and Klein’s Microbiology (Seventh Edition)- by Joanne M. Willey, Linda M. Sherwood, Christopher J. Woolverton. McGrawHill.
  2. Microbiology An Introduction (10th Edition)- by Gerard J. Tortora, Berdell R. Funke, Christine L. Case. Pearson.
  3. Text book of Microbiology (7th Edition)- by Ananthanereyan & Paniker, Publisher Universities press.

(e-book link: https://www.pdfdrive.com/textbook-of-microbiology-e177143667.html )

  1. Paniker’s Text book of Parasitology (8th Edition)- C. K. Jayaram Paniker. The Health Sciences Publisher.

(e-Book link: https://www.pdfdrive.com/panikers-textbook-of-medical-parasitology-e183895440.html )

 

Online Tutorial links:

  1. Fundamentals of Immunology: Innate Immunity and B-Cell Function

(Coursera link: https://www.coursera.org/learn/immunologyfundamentalsimmunitybcells )

  1. Fundamentals of Immunology: T Cells and Signaling

(Coursera link: https://www.coursera.org/learn/immunologyfundamentalstcellssignaling )

  1. Fundamentals of Immunology: Death by Friendly Fire

(Coursera link: https://www.coursera.org/learn/immunology-friendlyfire )

  1. Viruses & How to Beat Them: Cells, Immunity, Vaccines

(edX link: https://www.edx.org/course/viruses-how-to-beat-them-cells-immunity-vaccines)

  1. The Immune System: New Developments in Research

(edX link: https://www.edx.org/course/the-immune-system-new-developments-in-research-par)

 

 Simulation links for labs:

Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).

Online Source: Lab for life, Lecturio, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia

Session Plan

Session 1

Topic: Immunity: Concept of Innate and Adaptive immunity

Video on Classification of Immune System

https://www.youtube.com/watch?v=08PtttY6SRg

https://www.youtube.com/watch?v=m4QZ01wxDc4

https://www.youtube.com/watch?v=PzunOgYHeyg

Presentation on Innate and Adaptive Immunity

Innate & Adaptive Immunity

Session 2

Topic: Structure, Functions and Properties of Stem cell

Video on Properties of Stem Cells

https://www.youtube.com/watch?v=i2pyDBMglfM

TED Talk on future with Stem cells

https://www.youtube.com/watch?v=MvMUM-huMaI

Presentation on properties of Stem cells

Stem cells properties

Session 3

Topic: T cell, B cell, NK cell, Macrophage, Neutrophil, Eosinophil, Basophil, Mast cell, Dendritic cell

Video on Immune Cells

https://www.youtube.com/watch?v=9r0xzlpNjTw

Presentation on cells of Immune system

Cells of Immune System

Session 4

Topic: Bone Marrow, Thymus, Lymph Node, Spleen, GALT, MALT, CALT

Video on Organs and Tissues of Immune System

https://www.youtube.com/watch?v=2qeyGPCXBmM

Presentation on Organs of Immune System

Cells of Immune System

Session 5

Topic: Antigens: Characteristic

Video on APC

https://www.youtube.com/watch?v=LwLYGTS_3EI

Presentation on Antigens

Antigens

Session 6

Topic: Epitope; Haptens; Adjuvants

Video on Antigenic determinants

https://www.youtube.com/watch?v=x9-XdWO6x84

Presentation on Epitopes

Epitopes

Adjuvants: https://spark.adobe.com/page/xhF81F2UaVYzH/

Session 7

Topic: Antibody structure

Video on Antibody structure

https://www.youtube.com/watch?v=C_GRI3fxUWw

Presentation on Antibody structure

https://spark.adobe.com/page/NIWZhFAXAAqxt/

Session 8

Topic: Immunoglobulin classes

Video on Antibody classes

https://www.youtube.com/watch?v=v9jl9QpVz10

Presentation on Antibody classes

Antibody Structure

Session 9

Topic: Antigen-Antibody interaction, avidity & affinity

Video on Ag-Ab inetraction

https://www.youtube.com/watch?v=0oCqNUZlc0c

Presentation on Antigen- Antibody interaction

Ag- Ab interaction

Session 10

Topic: Serological Reactions

Video on Serological reactions

https://app.lecturio.com/#/lecture/s/8226/36272

Presentation on Serological reactions

Serological Reactions

Session 11

Session 12

Practice session

Topic: Performing Serological tests i.e.- VDRL (including Antigen Preparation)

Video on VDRL test

https://www.youtube.com/watch?v=cFRk6CoupDs

Procedure to perform VDRL test

https://microbenotes.com/venereal-disease-research-laboratory-vdrl-test/

.

Session 13

Practice session

Topic: Performing Serological tests i.e.- ASO (Antistreptolysin ‘O’)

Video on ASO test

https://www.youtube.com/watch?v=ahP-xBauddE

Procedure to perform ASO test

https://laboratoryinfo.com/aso/

Session 14

Practice session

Topic: Performing Serological tests i.e.- CRP Protein test

Video on CRP test

http://labsforlife.in/InstructionalVideo.aspx

Procedure to perform CRP test

https://microbiologyinfo.com/c-reactive-protein-crp-test-principle-uses-procedure-and-result-interpretation/

Session 15

Practice session

Topic: Performing Serological tests i.e.- CRP Protein test

Video on CRP test

http://labsforlife.in/InstructionalVideo.aspx

Procedure to perform CRP test

https://microbiologyinfo.com/c-reactive-protein-crp-test-principle-uses-procedure-and-result-interpretation/

Session 16

Practice session

Topic: Performing Serological tests i.e.- Rheumatoid Factor (RF)

Video on RF test

https://www.youtube.com/watch?v=X8vwC26f74M

Procedure to perform RF test

https://laboratoryinfo.com/rheumatoid-factor-rf-test/

Session 17

Practice session

Topic: Performing Serological tests i.e.- HBsAg

Video on HBsAg test

http://labsforlife.in/InstructionalVideo.aspx

Procedure to perform HBsAg test

http://premiermedcorp.com/wp-content/uploads/2013/08/product_documents/Infectious_Diseases/I10FRC30-1.pdf

Session 18

Topic: Principles of Precipitation

Video on Precipitation reaction

https://www.youtube.com/watch?v=Ew15yHuqzmY

Session 19

Topic: Agglutination reaction

Video on Agglutination reaction

https://www.youtube.com/watch?v=3W67OH3v2lU

Presentation on Agglutination reaction

Agglutination reaction

Session 20

Topic: Immunodiffusion

Video on Immunodiffusion

https://www.youtube.com/watch?v=hmK7yYr2T54

Slideshare link to Immunodiffusion

https://www.slideshare.net/suniu/immunodiffusion-principles-and-application

Session 21

Practice session

Topic: Performing Ouchterlony Double diffusion test

Video on Ouchterlony Double diffusion test

https://www.youtube.com/watch?v=hmK7yYr2T54&t=1s

Procedure to perform Ouchterlony Double diffusion test

http://vlab.amrita.edu/?sub=3&brch=70&sim=689&cnt=1

Session 22

Topic: Immunoelectrophoresis

Video on Immunoelectrophoresis

https://www.youtube.com/watch?v=zUGikX9ZB9U

Slideshare link to Immunoelectrophoresis

https://www.slideshare.net/SaajidaSultaana/rocket-immunoelectrophoresis

Session 23

Practice session

Topic: Precipitation in agarose gel

Video on Agarose gel preparation

https://www.youtube.com/watch?v=vq759wKCCUQ

https://www.youtube.com/watch?v=6mQGNDnOyH8

Procedure to perform Agarose gel preparation

http://premiermedcorp.com/wp-content/uploads/2013/08/product_documents/Infectious_Diseases/I10FRC30-1.pdf

Session 24

Session 25

Session 26

Session 27

Topic: ELISPOT

Video on ELISPOT

https://www.youtube.com/watch?v=opBf_5gsIMQ

Session 28

Topic: Western blotting

Video on Western Blotting

https://www.youtube.com/watch?v=VgAuZ6dBOfs&t=6s

Session 29

Practice session

Topic: Demonstration of Western blotting

Video on Western Blotting

https://www.youtube.com/watch?v=uFu8aie4QFI&t=11s

Procedure to perform Western Blotting

https://microbenotes.com/western-blotting-introduction-principle-and-applications/

Session 30

Practice session

Topic: Demonstration of Western blotting

Video on Western Blotting

https://www.youtube.com/watch?v=uFu8aie4QFI&t=11s

Procedure to perform Western Blotting

https://microbenotes.com/western-blotting-introduction-principle-and-applications/

Session 31

Topic: Role of complement system in immune response, Complements components

Video on Complement system

https://www.youtube.com/watch?v=DPNnZE4OtCM&t=541s

Presentation on Complement system

https://www.slideshare.net/SantoshYadav225/complement-system-65834753

Session 32

Topic: Complement Activation pathways

Video on Complement Activation pathways

https://www.youtube.com/watch?v=DPNnZE4OtCM&t=541s

Presentation on Complement Activation pathways

https://www.slideshare.net/SantoshYadav225/complement-system-65834753

Session 33

Topic: Cell mediated Immune response

Video on Cell mediated Immune response

https://www.youtube.com/watch?v=uIA5f4ah-Jg

Slideshare link to Cell mediated Immune response

https://www.slideshare.net/doctorrao/cell-mediated-immunity

Session 34

Topic: Humoral Immunity

Video on Humoral Immunity

https://www.youtube.com/watch?v=4s6IzVbyq-4

Presentation on Humoral Immunity

https://www.slideshare.net/doctorrao/humoral-immunity

Session 35

Topic: Monoclonal antibodies: Production, MAb characterization and applications

Video on Monoclonal Antibodies

https://www.youtube.com/watch?v=5AXApBbj1ps

Slideshare link to Monoclonal Antibodies

https://www.slideshare.net/LAKSHMIDEEPTHIGEDELA/monoclonal-antibodies-198366965

Session 36

Topic: Autoimmunity

Video on Autoimmunity

https://www.youtube.com/watch?v=JkgtKknaBXM

TEDx Talks on RA

https://www.youtube.com/watch?v=BtqUBp5WooI

Presentation on Autoimmunity

Autoimmunity

Session 37

Topic: Types of Hypersensitivity with examples

Video on Hypersensitivity types

https://www.youtube.com/watch?v=nQYATo46FfY&t=3s

Presentation on Hypersensitivity

Hypersensitivity

Session 38

Topic: DiGeorge syndrome

Video on DiGeorge syndrome

https://www.youtube.com/watch?v=YdDs92gaWl8

Session 39

Topic: Chediak- Higashi syndrome

Video on Chediak- Higashi syndrome

https://www.youtube.com/watch?v=ZY3tXJmnoro

Session 40

Topic: Leukocyte adhesion deficiency

Video on Leukocyte adhesion deficiency

https://www.youtube.com/watch?v=GkmxGIQe9Yw

Session 41

Topic: Congenital disorders of Glycosylation

Video on CGD

https://www.youtube.com/watch?v=xMBC-OoKjwM

Session 42

Topic: Introduction to medical Parasitology

Video on medical Parasitology

https://www.youtube.com/watch?v=7cSRZEl3GbI

Presentation on Parasitology

Parasitology

Session 43

Topic: Morphology of Parasites

Video on Morphology of Protozoa

https://www.youtube.com/watch?v=aDxVFoTrMwg

Presentation on Morphology of Protozoa

Morphology of Protozoa

Session 44

Topic: General Characteristics of Parasites

Video on Characteristics of Parasites

https://www.youtube.com/watch?v=zZAQPwsG3tM

Session 45

Topic: Classification of Parasites

Video on Classification of Parasites

https://www.youtube.com/watch?v=7cSRZEl3GbI

Slideshare link on Classification of Parasites

https://www.slideshare.net/shahiraahmed/classification-of-medical-parasites

Session 46

Topic: Laboratory diagnosis of different human pathogenic parasites

Video on Laboratory diagnosis of different human pathogenic parasites

https://app.lecturio.com/#/course/s/8286

Session 47

Practice session

Topic: Identification of adult worms from models or slide’s

Video on Identification of adult worms from models or slide’s

https://www.youtube.com/watch?v=39BDI_lqq9I

https://www.youtube.com/watch?v=T13cIxaG4CU

Session 48

Practice session

Topic: Identification of different parasites, their morphology from slide’s

Video on Identification of different parasites, their morphology from slide’s

https://www.youtube.com/watch?v=39BDI_lqq9I

https://www.youtube.com/watch?v=WPP7AjmStBg&t=4s

https://www.youtube.com/watch?v=ihSnTKLUazQ

Procedure for Identification of different parasites, their morphology from slide’s

https://www.slideshare.net/Prbn/lab-dia-of-parasite

Session 49

Topic: Examination of faeces for ova and cysts

Video on Examination of faeces for ova and cysts

https://www.youtube.com/watch?v=aVe2oSE_da8

https://www.youtube.com/watch?v=_ePqcdDKCe0

https://www.youtube.com/watch?v=-iI2PxmHxuo

Session 50

Practice session

Topic: Routine Stool examination for detection of intestinal parasites

Video on Routine Stool examination for detection of intestinal parasites

https://www.youtube.com/watch?v=MRzUXg8kFiY

https://www.youtube.com/watch?v=_ePqcdDKCe0&t=36s

Session 51

Topic: Concentration methods

Video on Concentration methods

https://www.youtube.com/watch?v=-iI2PxmHxuo

https://www.youtube.com/watch?v=oEKBYyTE2Xg

Session 52

Practice session

Topic: Concentration techniques for demonstration of Ova

Video on Concentration techniques for demonstration of Ova

https://www.youtube.com/watch?v=qLnpo5dveV4

https://www.youtube.com/watch?v=GTJzxZRo49A

Session 53

Topic: Blood smear examination for Parasites

Video on Blood smear examination for Parasites

https://www.youtube.com/watch?v=acoALifVvb8

https://www.youtube.com/watch?v=WPP7AjmStBg

Case Studies

Case Studies

Our Main Teachers

Dr. Soumya Jal

Associate Professor
VIEW PROFILE

Dr. Soumya Jal is working at Centurion University of Technology and Management since 2018, prior to which she was associated with Rama Devi Women’s University as a visiting faculty.  She has completed her Ph. D. in Medical Biotechnology from VIT University, Vellore (2016). Her research area includes fields such as, Applied and Clinical Microbiology, Molecular […]