Immunology and Virology
Teacher
Dr. Soumya Jal
Category
Core Courses
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Course Name : Immunology and Virology
Code(Credit): CUTM1719 (3-2-0)
Course Objectives
- Understanding the concept of Innate & adaptive immune system; complement system; Hypersensitivity
- Clinically relevant serological analysis for deeper understanding of antigen-antibody interaction
- To understand the concept of cells of immune system and organs of immune system
- To understand the properties of virus, diagnosis of important viruses and vaccination
Learning Outcomes
- The student will learn the application of Immunology in disease diagnosis.
- Complement system followed by the body on encountering an Antigen.
- Immune Response produced on encounter with foreign body.
- The students will learn the role of immunity in fighting disease, along with consequence of undesirable expression of immune system such as, hypersensitivity and auto immune disease.
- They will gather knowledge regarding the properties, diagnosis of virus and vaccination against them.
Course Syllabus
Module – 1
Immunity: Concept of Innate and Adaptive immunity.
Immune Cells and Organs: Structure, Functions and Properties of: Immune Cells – Stem cell, T cell, B cell, NK cell, Macrophage, Neutrophil, Eosinophil, Basophil, Mast cell, Dendritic cell; and Immune Organs – Bone Marrow, Thymus, Lymph Node, Spleen, GALT, MALT, CALT
Antigens: Characteristics, Hapten, Epitopes, Adjuvants;
Antibody: Structure & its classes.
Antigen-Antibody interaction, avidity & affinity.
Serological Reactions: Principles of Precipitation, Agglutination, Immunodiffusion, Immunoelectrophoresis, ELISA, ELISPOT, Western blotting, Immunofluoresence, Immunoelectron microscopy.
Practice:
Performing Serological tests:
Widal test
VDRL test
ASO test
C-Reactive Protein test
Rheumatoid factor (RF) test
Precipitation in agarose gel
Performing Ouchterlony Double diffusion test
Demonstration of SDS-PAGE
Demonstration of ELISA
Demonstration of Western blotting
Module – 2
Complement System: Role of complement system in immune response, Complement components and Activation pathways.
Immune Response: Cell mediated and humoral Immunity.
Monoclonal antibodies: Production, characterization and applications.
Types of Autoimmunity and Hypersensitivity with examples; Immunodeficiencies, SCID, DiGeorge syndrome, Chediak- Higashi syndrome, Leukocyte adhesion deficiency, CGD
Module-3
Virus: General properties, concept of viroids, virusoids, satellite viruses and Prions.
Structure of Viruses: Capsid symmetry, enveloped and non-enveloped viruses.
Isolation, purification and cultivation of viruses.
Serological diagnosis of virus infections.
Arthropod borne and rodent borne virus diseases, Picorna viruses and diseases, Hepatitis viruses, Rabies and other neuro viruses, Orthomyxo and paramyxo viruses, Coronaviridae, Pox, Adeno, Herpes, Reo, Rota virus, HIV Viruses, Oncogenic viruses.
Vaccines: their Preparation and their immunization schedules.
Practice:
Serological diagnosis of virus borne diseases:
Hepatitis
HIV
Suggested Readings:
- Kuby's Immunology (7th Ed) - by J. Owen, J. Punt, S. Strandford. Macmillan Higher Education, England.
(e-book link: https://www.pdfdrive.com/kuby-immunology-7th-edition-2013-e44842271.html )
- Roitt's Essential Immunology (13th Ed)- by Seamus J. Martin, Dennis R. Burton, Ivan M. Roitt. Wiley Blackwell.
(e-book link: http://dl.mehrsys.ir/pdf-books/Roitt_s%20Essential%20Immunology%20Thirteenth%20Edition(www.myuptodate.com).pdf )
- Prescott, Harley, and Klein’s Microbiology (Seventh Edition)- by Joanne M. Willey, Linda M. Sherwood, Christopher J. Woolverton. McGrawHill.
- Microbiology An Introduction (10th Edition)- by Gerard J. Tortora, Berdell R. Funke, Christine L. Case. Pearson.
- Text book of Microbiology (7th Edition)- by Ananthanereyan & Paniker, Publisher Universities press.
(e-book link: https://www.pdfdrive.com/textbook-of-microbiology-e177143667.html )
- Fundamentals of Molecular Virology (2nd Edition)- by Nicholas H. Acheson, Wiley Publishers.
(e-Book link: https://www.pdfdrive.com/fundamentals-of-molecular-virology-d157673335.html )
- Principles of Virology (4th Edition)- by Jane Flint, Glenn F. Rall, Vincent R. Racaniello, Anna Marie Skalka, Lynn W. Enquist. ASM Press, Washington, DC.
(e-Book link: https://www.pdfdrive.com/principles-of-virology-d158020773.html )
Online Tutorial links:
- Fundamentals of Immunology: Innate Immunity and B-Cell Function
(Coursera link: https://www.coursera.org/learn/immunologyfundamentalsimmunitybcells )
- Fundamentals of Immunology: T Cells and Signaling
(Coursera link: https://www.coursera.org/learn/immunologyfundamentalstcellssignaling )
- Fundamentals of Immunology: Death by Friendly Fire
(Coursera link: https://www.coursera.org/learn/immunology-friendlyfire )
- Viruses & How to Beat Them: Cells, Immunity, Vaccines
(edX link: https://www.edx.org/course/viruses-how-to-beat-them-cells-immunity-vaccines)
- The Immune System: New Developments in Research
(edX link: https://www.edx.org/course/the-immune-system-new-developments-in-research-par)
Simulation links for labs:
Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).
labsforlife (http://labsforlife.in/)
Online Source: Lecturio, Udemy, Coursera, article library, NCBI, Study.com, You tube, unacademy, academia
Session Plan
Session 1
Topic: Immunity: Concept of Innate and Adaptive immunity
Video on Classification of Immune System
https://www.youtube.com/watch?v=08PtttY6SRg
https://www.youtube.com/watch?v=m4QZ01wxDc4
https://www.youtube.com/watch?v=PzunOgYHeyg
Presentation on Innate and Adaptive Immunity
Session 2
Topic: Structure, Functions and Properties of Stem cell
Video on Properties of Stem Cells
https://www.youtube.com/watch?v=i2pyDBMglfM
TED Talk on future with Stem cells
https://www.youtube.com/watch?v=MvMUM-huMaI
Presentation on properties of Stem cells
Session 3
Topic: T cell, B cell, NK cell, Macrophage, Neutrophil, Eosinophil, Basophil, Mast cell, Dendritic cell
Video on Immune Cells
https://www.youtube.com/watch?v=9r0xzlpNjTw
Presentation on cells of Immune system
Session 4
Topic: Bone Marrow, Thymus, Lymph Node, Spleen, GALT, MALT, CALT
Video on Organs and Tissues of Immune System
https://www.youtube.com/watch?v=2qeyGPCXBmM
Presentation on Organs of Immune System
Session 5
Topic: Antigens: Characteristic
Video on APC
https://www.youtube.com/watch?v=LwLYGTS_3EI
Presentation on Antigens
Session 6
Topic: Epitope; Haptens; Adjuvants
Video on Antigenic determinants
https://www.youtube.com/watch?v=x9-XdWO6x84
Presentation on Epitopes
Session 7
Topic: Antibody structure
Video on Antibody structure
https://www.youtube.com/watch?v=C_GRI3fxUWw
Presentation on Antibody structure
Session 8
Topic: Immunoglobulin classes
Video on Antibody classes
https://www.youtube.com/watch?v=v9jl9QpVz10
Presentation on Antibody classes
Session 9
Topic: Antigen-Antibody interaction, avidity & affinity
Video on Ag-Ab inetraction
https://www.youtube.com/watch?v=0oCqNUZlc0c
Presentation on Antigen- Antibody interaction
Session 10
Topic: Serological Reactions
Video on Serological reactions
https://app.lecturio.com/#/lecture/s/8226/36272
Presentation on Serological reactions
Session 11
Practice session
Topic: Performing Serological tests i.e.- Widal test
Video on Widal test
http://labsforlife.in/InstructionalVideo.aspx
Procedure to perform Widal test
Session 12
Practice session
Topic: Performing Serological tests i.e.- VDRL (including Antigen Preparation)
Video on VDRL test
https://www.youtube.com/watch?v=cFRk6CoupDs
Procedure to perform VDRL test
https://microbenotes.com/venereal-disease-research-laboratory-vdrl-test/
Session 13
Practice session
Topic: Performing Serological tests i.e.- ASO (Antistreptolysin ‘O’)
Video on ASO test
https://www.youtube.com/watch?v=ahP-xBauddE
Procedure to perform ASO test
Session 14
Practice session
Topic: Performing Serological tests i.e.- CRP Protein test
Video on CRP test
http://labsforlife.in/InstructionalVideo.aspx
Procedure to perform CRP test
Session 15
Practice session
Topic: Performing Serological tests i.e.- CRP Protein test
Video on CRP test
http://labsforlife.in/InstructionalVideo.aspx
Procedure to perform CRP test
Session 16
Practice session
Topic: Performing Serological tests i.e.- Rheumatoid Factor (RF)
Video on RF test
https://www.youtube.com/watch?v=X8vwC26f74M
Procedure to perform RF test
Session 17
Practice session
Topic: Performing Serological tests i.e.- HBsAg
Video on HBsAg test
http://labsforlife.in/InstructionalVideo.aspx
Procedure to perform HBsAg test
Session 18
Topic: Principles of Precipitation
Video on Precipitation reaction
Session 19
Topic: Agglutination reaction
Video on Agglutination reaction
https://www.youtube.com/watch?v=3W67OH3v2lU
Presentation on Agglutination reaction
Session 20
Topic: Immunodiffusion
Video on Immunodiffusion
https://www.youtube.com/watch?v=hmK7yYr2T54
Slideshare link to Immunodiffusion
https://www.slideshare.net/suniu/immunodiffusion-principles-and-application
Session 21
Practice session
Topic: Performing Ouchterlony Double diffusion test
Video on Ouchterlony Double diffusion test
https://www.youtube.com/watch?v=hmK7yYr2T54&t=1s
Procedure to perform Ouchterlony Double diffusion test
Session 22
Topic: Immunoelectrophoresis
Video on Immunoelectrophoresis
https://www.youtube.com/watch?v=zUGikX9ZB9U
Slideshare link to Immunoelectrophoresis
https://www.slideshare.net/SaajidaSultaana/rocket-immunoelectrophoresis
Session 23
Practice session
Topic: Precipitation in agarose gel
Video on Agarose gel preparation
https://www.youtube.com/watch?v=vq759wKCCUQ
https://www.youtube.com/watch?v=6mQGNDnOyH8
Procedure to perform Agarose gel preparation
Session 24
Practice session
Topic: Demonstration of SDS-PAGE
Video on SDS-PAGE
https://www.youtube.com/watch?v=pnBZeL8nFEo&t=2s
Procedure to perform SDS-PAGE
Session 25
Topic: ELISA
Video on ELISA Technique
https://www.youtube.com/watch?v=CaEwcf5ntWw
Slideshare link to ELISA
Session 26
Practice session
Topic: Demonstration of ELISA
Video on ELISA
https://www.youtube.com/watch?v=qvsBoEvJxK0&t=1s
https://www.youtube.com/watch?v=ECjGF1qMx6w&t=5s
Procedure to perform ELISA
https://microbiologynotes.com/elisa-principle-types-and-applications/
Session 27
Session 28
Session 29
Practice session
Topic: Demonstration of Western blotting
Video on Western Blotting
https://www.youtube.com/watch?v=uFu8aie4QFI&t=11s
Procedure to perform Western Blotting
https://microbenotes.com/western-blotting-introduction-principle-and-applications/
Session 30
Topic: Immunofluoresence, Immunoelectron microscopy
Video on Immunofluorescence
https://www.youtube.com/watch?v=pteO6FRWo3g
Slideshare link to Immunoelectron microscopy
https://www.slideshare.net/meghnathiruveedi/immunoelectron-microscopy
Session 31
Topic: Role of complement system in immune response, Complements components
Video on Complement system
https://www.youtube.com/watch?v=DPNnZE4OtCM&t=541s
Slideshare link to Complement system
https://www.slideshare.net/SantoshYadav225/complement-system-65834753
Session 32
Topic: Complement Activation pathways
Video on Complement Activation pathways
https://www.youtube.com/watch?v=DPNnZE4OtCM&t=541s
Slideshare link to Complement Activation pathways
https://www.slideshare.net/SantoshYadav225/complement-system-65834753
Session 33
Topic: Cell mediated Immune response
Video on Cell mediated Immune response
https://www.youtube.com/watch?v=uIA5f4ah-Jg
Slideshare link to Cell mediated Immune response
Session 34
Topic: Humoral Immunity
Video on Humoral Immunity
https://www.youtube.com/watch?v=4s6IzVbyq-4
Presentation on Humoral Immunity
Session 35
Topic: Monoclonal antibodies: Production, MAb characterization and applications
Video on Monoclonal Antibodies
https://www.youtube.com/watch?v=5AXApBbj1ps
Slideshare link to Monoclonal Antibodies
https://www.slideshare.net/LAKSHMIDEEPTHIGEDELA/monoclonal-antibodies-198366965
Session 36
Topic: Autoimmunity
Video on Autoimmunity
https://www.youtube.com/watch?v=JkgtKknaBXM
TEDx Talks on RA
https://www.youtube.com/watch?v=BtqUBp5WooI
Presentation on Autoimmunity
Session 37
Topic: Types of Hypersensitivity with examples
Video on Hypersensitivity types
https://www.youtube.com/watch?v=nQYATo46FfY&t=3s
Presentation on Hypersensitivity
Session 38
Session 39
Topic: Chediak- Higashi syndrome
Video on Chediak- Higashi syndrome
Session 40
Topic: Leukocyte adhesion deficiency
Video on Leukocyte adhesion deficiency
Session 41
Topic: Congenital disorders of Glycosylation
Video on CGD
Session 42
Topic: Virus: General properties, concept of viroids, virusoids, satellite viruses and Prions
Video on Virus: Properties
https://www.youtube.com/watch?v=8FqlTslU22s
Slideshare link to Virus: Properties
https://www.slideshare.net/raghunathp/general-properties-of-viruses
Session 43
Topic: Structure of Viruses: Capsid symmetry, enveloped and non-enveloped viruses, Isolation, purification and cultivation of viruses
Video on Structure of Virus
https://www.youtube.com/watch?v=XNs7VSnoskw
Slideshare link to Structure of Virus
https://www.slideshare.net/doctorrao/virus-structure-and-replication
Session 44
Topic: Serological diagnosis of virus infections
Video on Serological diagnosis of virus infections
https://www.youtube.com/watch?v=K5C0D3A6FGM
Slideshare link to Serological diagnosis of virus infections
https://www.slideshare.net/doctorrao/diagnosis-of-viral-infections-basics
Session 45
Topic: Arthropod borne and rodent borne virus diseases
Video on Arthropod borne and rodent borne virus diseases
https://www.youtube.com/watch?v=e7ulJEuu-Zo
Slideshare link to Arthropod borne Viral infection
https://www.slideshare.net/doctortvrao/arthropodborne-viral-infections-by-drtvrao-md
Session 46
Topic: Picorna viruses and diseases
Video on Picorna Virus
https://www.youtube.com/watch?v=_uDKQSELyno
Slideshare link on Picorna Virus
Session 47
Topic: Hepatitis viruses
Video on Hepatitis
https://www.youtube.com/watch?v=Fpeg0joWaEk
Slideshare link to Hepatitis
https://www.slideshare.net/d.santiago/hepatitis-presentation-7424129
WHO statistical analysis on Hepatitis
https://www.slideshare.net/who_europe/slides-hepatitis-enfinal
Session 48
Practice session
Topic: Performing Serological test on HbSAg
Video on HbSAg
http://labsforlife.in/InstructionalVideo.aspx
Procedure to perform HbSAg test
https://www.cdc.gov/nchs/data/nhanes/nhanes_11_12/HEPBD_G_met_Hepatitis%20B%20surface%20antigen.pdf
Session 49
Topic: Rabies and other neuro viruses
Video on Rabies virus
Session 50
Topic: Orthomyxo and paramyxo viruses
Video on Orthomyxo viruses
https://www.youtube.com/watch?v=m9D5uO4TFB4
Video on Paramyxo viruses
Session 51
Topic: Coronaviridae
Video on Coronaviridae
https://www.youtube.com/watch?v=jWMUBouaqb0
https://www.youtube.com/watch?v=I-Yd-_XIWJg
https://www.youtube.com/watch?v=DD_wdvwN9rg
WHO information on COVID-19
MoHFW information on COVID-19
https://www.mohfw.gov.in/pdf/2COVID19PPT_25MarchPPTWithAnimation.pdf
Session 52
Session 53
Session 54
Session 55
Topic: Reo virus, Rota Virus
Video on Reo virus, Rota Virus
Session 56
Topic: HIV Viruses
Videos on HIV virus
https://www.youtube.com/watch?v=r8CBFEyODL8
https://www.youtube.com/watch?v=5g1ijpBI6Dk
https://www.youtube.com/watch?v=ng22Ucr33aw
WHO information on HIV virus
https://www.who.int/news-room/fact-sheets/detail/hiv-aids
CDC information on HIV virus
Session 57
Practice session
Topic: Performing HIV test
Video on HIV test procedure
http://labsforlife.in/InstructionalVideo.aspx
Procedure to perform HIV test
Session 58
Session 59
Topic: Vaccines: their Preparation and their immunization schedules
Videos on Vaccination and its schedule
https://www.youtube.com/watch?v=Atrx1P2EkiQ
https://www.youtube.com/watch?v=TmXsK0hON6s
https://www.youtube.com/watch?v=J0SvutsCHrU
WHO information on Vaccination
https://www.who.int/immunization/policy/immunization_tables/en/
CDC information on Vaccination
https://www.cdc.gov/vaccines/schedules/hcp/imz/child-adolescent.html
Case Studies
Case Studies
Our Main Teachers
Dr. Soumya Jal is working at Centurion University of Technology and Management since 2018, prior to which she was associated with Rama Devi Women’s University as a visiting faculty. She has completed her Ph. D. in Medical Biotechnology from VIT University, Vellore (2016). Her research area includes fields such as, Applied and Clinical Microbiology, Molecular […]