Immunology

Teacher

Dr Sitaram Swain

Category

Core Courses

Course Attendees

Still no participant

Course Reviews

Still no reviews

Course Name : Immunology

Code(6) :CUTM1705(3-1-2)

Course Objectives

  • The primary objective of this course is to help students develop knowledge and skills related to health and disease and role of immune system.students are taught immunology so as to develop understanding of the subject, such as functioning the immune system, the molecular and cellular components and pathways that protect an organism from infectious agents

Learning Outcomes

  • •Understand the immunomodulatory strategies essential for generating or suppressing immune responses as required in hypersensitivity reactions, transplantation, autoimmune diseases and cancer.
    • Students will know the principle and application of various immune techniques and they can Will be able to make a strategy for immunological research and execute it.

Course Syllabus

Module:I Immunity: Historical Perspective, Cellular and humoral immunity. Role of immunologically important organs and cells, bone marrow, thymus, spleen and lymphocytes. Formation and functions of T and B Lymphocytes. Helper T-cells and killer T-cells. Macrophages.
Module:II Complement activation by classical, alternate and MB lectin pathway, biological consequences of complement activation, regulation and complementdeficiencies.
Module: III Antigens: Definition, epitopes, haptens, carriers, adjuvants, antigenicity. Antibodies: Definition, types and structure of immunoglobulins (IgG – light chain, heavy chain, hyper variable region, constant domains, Fab and Fc). Effector functions of antibody, antigenic determinants on Ig, Ig super family. Monoclonal antibodies production and applications.
Module: IV Major histocompatibility complex (MHC) genes and products – Polymorphism of MHC genes, role of MHC antigens in immune responses, MHC antigens in transplantation.
Module V: Antigen–antibody reaction in vitro: Formation of antigen-antibody complex. Application of immunodiffusion, RIA, ELISA (Basic principles only).
Module:VI Immunization: Vaccination – vaccines and their preparations, primary and secondary immune response, active and passive immunization, types of vaccines, immunosuppressive therapy.
Module:VII Autoimmunity and hypersensitivity: Self-tolerance and possible mechanisms of induction of autoimmunity, Organ specific and systemic autoimmune diseases, Types of hypersensitivity.Immunological disorders: Allergy and AIDS. Structure of HIV, mode of transmission.
Practical content
1. Isolation of lymphocytes from blood / spleen.
2. Ouchterlony double immunodiffusion (DID)
3. Purification ofimmunoglobulins
4. Demonstration of Western Blotting
5. Assays based on agglutination reactions - Blood typing (active) and passive agglutination.
6. Enzyme linked immunosorbent assay (ELISA) & DOTELISA
Suggested Readings:
• I.M. Riott, J. Brostoff, D. Male “Immunology” 3rd edn. W.H. Freeman and Pub. Company, USA.
• J. Kuby “Immunology” 3rd edn., Mosby Year Book Co., England
• Introduction to Immunology – NandiniShetty (2003)
• Immunology – Janis Kuby – W. H. Freeman and Co. 7th edition (2019)
• Janeway’sImmunobiology 2012 8th ed., Murphy, K., Mowat, A., and Weaver, C.T.,
• Garland Science (London & New York), ISBN:978-0-8153-4243-4

Session Plan

Session 1

Immunity: Historical Perspective.

Basic immunology

Session 7

Formation and functions of  B Lymphocytes

Function of B lymphocyte

Session 6

Formation and functions of T  Lymphocytes

Function of T lymphocytes

Session 5

Bone marrow, thymus, spleen and lymphocytes

Lymphoid Organs

Session 4

Role of immunologically important cells

Important cells

Session 3

Role of immunologically important organs.

Important organs

Session 2

Cellular and humoral immunity

Cellular and humoral immunity

Session 8

Practice:Isolation of lymphocytes from blood

Practice:

Session 9

Helper T-cells and killer T-cells. Macrophages.

helper T cells 

Session 10

Flipped class: Cells of the immunesystem

https://www.youtube.com/watch?v=k9QAyP3bYmc

Session 11

Complement activation system

 

https://www.youtube.com/watch?v=DPNnZE4OtCM

Session 12

classical, alternate and MB lectin pathway

https://www.youtube.com/watch?v=d6qFPegEYV0

Session 13

biological consequences of complement activation.

https://www.youtube.com/watch?v=IUDSWPvfHgU

Session 14

Regulation of complement system and its function

 

https://www.youtube.com/watch?v=N3L4kQqsGPQ

Session 15

Regulation and complementdeficiencies

 

https://www.youtube.com/watch?v=Toddr4AUX6I

Session 16

Practice: Ouchterlony double immunodiffusion (DID)

https://www.youtube.com/watch?v=hmK7yYr2T54&t=74s

Session 17

Session 18

Epitopes, haptens, carriers

https://www.youtube.com/watch?v=YIG3BPfG-0Y

Session 19

Session 20

Antibodies: Definition, types and structure of immunoglobulins

https://www.youtube.com/watch?v=C_GRI3fxUWw

Session 21

Flipped class: Antibodies: structure of immunoglobulins

https://www.youtube.com/watch?v=C_GRI3fxUWw

Session 22

Practice:Purification ofimmunoglobulins

https://www.youtube.com/watch?v=Rxzwb6DRdZU

Session 23

IgG – light chain, heavy chain, hyper variable region, constant domains, Fab and Fc

https://www.youtube.com/watch?v=NTFvzwdIv4k

Session 24

Session 25

Effector functions of antibody.

https://www.youtube.com/watch?v=N3L4kQqsGPQ&t=41s

Session 26

Antigenic determinants on Ig

https://www.youtube.com/watch?v=HjiJZo1NQno

Session 27

Session 28

Monoclonal antibodies production and applications.

https://www.youtube.com/watch?v=8iyrbv1JauY

Session 29

Flipped class:Production of Monoclonal antibodies production.

https://www.youtube.com/watch?v=U76Ll3OuBsU

Session 30

Practice:Purification of immunoglobulins

https://www.youtube.com/watch?v=IpN0JzMylYM&t=181s

Session 31

Major histocompatibility complex (MHC) genes

 

https://www.youtube.com/watch?v=t9TvTmddanE

Session 32

Session 33

Polymorphism of MHC genes and its products

https://www.youtube.com/watch?v=zkx2TvYCAR8

Session 34

Role of MHC antigens in immune responses

https://www.youtube.com/watch?v=t81EjpkCiss

Session 35

MHC antigens in transplantation.

https://www.youtube.com/watch?v=CirqxHmRbjA

Session 36

Flipped class: Transplantation.

https://www.youtube.com/watch?v=CirqxHmRbjA&t=35s

Session 37

Pactice: Demonstration of western blotting.

https://www.youtube.com/watch?v=htOskgNW5UE

Session 38

Antigen–antibody reaction in vitro

https://www.youtube.com/watch?v=3ztaL2NDIhM&t=51s

Session 39

flipped class: Different antigen-antibody reaction

https://www.youtube.com/watch?v=3ztaL2NDIhM

Session 44

Immunization: Vaccination – vaccines and their preparations

 

https://www.youtube.com/watch?v=qpjh3qMm2Gs

Session 42

Session 41

Session 40

Application of immunodiffusion

https://www.youtube.com/watch?v=4vgyrT0U7S8

Session 45

primary and secondary immune response

https://www.youtube.com/watch?v=VPoffYhtOXY

Session 46

primary and secondary immune response,active and passive immunization

https://www.youtube.com/watch?v=LYZzvrg_qIM

Session 47

Session 52

Organ specific and systemic autoimmune diseases, Types of hypersensitivity.Immunological disorders: Allergy and AIDS

https://www.youtube.com/watch?v=jXTW4F-8jd4

Session 51

Structure of HIV, mode of transmission.

https://www.youtube.com/watch?v=GyofqO1TRjU

Session 50

Mechanism of  HIV transmission.

https://www.youtube.com/watch?v=dKG-IZLrwlU

Session 49

Autoimmunity and hypersensitivity:Self-tolerance and possible mechanisms of induction of autoimmunity

https://www.youtube.com/watch?v=vDwNpDT-8L0&t=96s

https://www.youtube.com/watch?v=CjRAkawBRaI

Session 48

Case Studies

Case Studies

Our Main Teachers

Dr Sitaram Swain

Assistant Professor
VIEW PROFILE

Dr Sitaram Swain has received PhD degree from Utkal University in the year 2017. He is also trained and experienced from TATA Memorial Hospital, Mumbai, India. He has experienced with cell line culture, clinical biochemistry, immuno-fixation, immunoassay, cancer cytogenetic, serum protein electrophoresis and tumour marker analysis. He has qualified NET(ICAR-ASRB) in Animal biotechnology. He has […]