| COs | Course outcomes | Mapping COs with POs (High-3, Medium-2, Low-1) |
| CO1 | Explain the definition, classification, biological function, structure and interactions of Biomolecules. | PO1 ( 3) |
| CO2 | Analyse the functioning of life at cellular level. | PO2(2) |
| CO3 | Discuss and differentiate the basic structure and function of cell components in prokaryotes and eukaryotes cells. | Po3(2) |
Introduction to Biotechnology (CUTM 2367) (3-0-0)
Teacher
Dr. Ranjan Kumar Sahoo
Category
Core Courses
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Course Objective
- To introduce student’s basic knowledge about Biotechnology
Course Outcome
To impart a sound knowledge on the principles of Biotechnology involving the different application oriented topics required for all engineering branches
Module I: Biochemistry
Component of the cell, structure and biochemical functions
Module II: Biomolecules
Carbohydrates, Lipids, Proteins, Nucleic acids, Structure and classification of enzymes.
Module III: Cell Biology
Eukaryotic and Prokaryotic cells, Cell cycle-Mitosis and Meiosis, Cell fractionation and flow cytometry
Module IV: Introduction to nucleic acids
Nucleic acids as genetic material, structure and physiochemical properties of elements in DNA and RNA, Biological significance of differences in DNA and RNA
Module V: Immunology
Cells of immune system, Development, maturation, activation and differentiation of T cells and B cells, Phagocytosis process
Module VI: Introduction to cloning
Overview of cloning, Purification and separation of Nucleic acids-cutting and joining DNA and vectors, analysis of gene expression, applications.
ModuleVII: Biotechnology Application
Industrial production, Drug Discovery and development, GMO, Bioremediation.
List of Experiments:
- pH measurements and preparation of buffers.
- Microscopic study of cell and cell organelles
- Blood grouping
- DNA and RNA isolation
- Restriction enzyme digestion of plasmid DNA
Reference:
Text books:
- Lehninger A.L., Nelson D.L. and M.M. Principles of Biochemistry.CBS publishers and distributers.
- Murray R.K., Granner D.K., Mayes P.A and Rodwell V.W.Harpers Biochemistry. Appleton and Lange, Stanford, Conneticut.
Reference Books:
- Lodish,Harvey et al., “Molecular Cell Biology”, 6th W.H Freeman,2008.
- Alberts,Bruce, “Molecular Biology of Cell”,5th Edition, Garland Science,2008.
- Satyanarayana,U. “Biotechnology” Books & Allied(P) Ltd.,2019.
- Friefelder, David. “Molecular Biology”.Narosa Publications, 2016.
Session 7
Structure and classification of enzymes
Session 10
Differences between Eukaryotic and Prokaryotic cells
Session 16
Structure and physiochemical properties of elements in DNA and RNA
http://https://www.youtube.com/watch?v=SZ0vT27sStc
Session 17
Biological significance of differences in DNA and RNA
Session 19
Development, maturation, activation and differentiation of T cells and B cells
Session 24
http://https://www.youtube.com/watch?v=Hv5flUOsE0s
Analysis of gene expression, applications
Session 30
Practice-2
Session 29
Practice-1
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