Medical Instrumentation & Techniques
Teacher
Susmita Chakrabarty
Category
Core Courses
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Course Name : Medical Instrumentation & Techniques
Code(Credit) : CUTM1730 (2-2-0)
Course Objectives
- To learn the principle, instrumentation & application of Microscopy
- Principle, instrumentation & application of Centrifugation
- Principle of Spectroscopy
Learning Outcomes
- After completion of the course the student will be efficient in handling the microscopy equipment’s.
- They will also be able to have idea about handling instruments like centrifuge, spectrophotometer, chromatography, flow cytometer, Automated and semi- automated Biochemistry analyzer.
- The conceptual understanding of the subject provides opportunities for skill enhancement and scopes for higher education.
Course Syllabus
Module -I (12 hrs)
Microscopic techniques: Principle, Instrumentation, Specimen preparation and Application: Phase–contrast microscopy, fluorescene microscopy, polarization microscopy, electron microscopy (Scanning and Transmission);
Bacterial Colony Counter (Principle and working). Laminar Air Flow (Principle and working technique).
Practice: Demonstration of different Microscopes with their operation and maintain technique.
Module- II (14 hrs)
Colorimeter: Principle and Instrumentation;
Spectrophotometry: Ultraviolet, Mass spectrophotometry; Flame photometry.
Centrifugation: Principle; Preparative, Analytical, Density gradient centrifugation.
Cytometry: Types, Flow cytometry and its applications.
Practice: Operation, Demonstration and Quality control of Centrifuge, UV-Vis spectrometer, Colorimeter.
Module- III ( 14 hrs)
Microtomy: Sectioning, Staining. Application.
Principle and Application of: Fully Automated Biochemistry Analyser, Semi- automated Biochemistry Analyser, Coagulometer.
Principle, working and uses of: Incubator, Hot air oven, Autoclave.
Practice: Demonstration of Auto/ Semi auto Analyzer; Working procedure of microtome, Incubator, Hot air oven, autoclave and others
Suggested Readings:
- Wilson K and Walker J. (2010). Principles and Techniques of Biochemistry and Molecular Biology. 7th Ed., Cambridge University Press.
(e-Book link: https://www.pdfdrive.com/principles-and-techniques-of-biochemistry-and-molecular-biology-e174866056.html )
- Nelson DL and Cox MM. (2008). Lehninger Principles of Biochemistry, 5th Ed., W.H. Freeman and Company.
(e-Book link: https://www.pdfdrive.com/lehninger-principles-of-biochemistry-5th-edition-d164892141.html )
- Willey MJ, Sherwood LM & Woolverton C J. (2013). Prescott, Harley and Klein’s Microbiology. 7thEd., McGraw Hill.
(e-Book link: https://www.pdfdrive.com/prescott-harley-and-kleins-microbiology-7th-ed-e188166539.html )
- Labs for Life
(e-source link: http://labsforlife.in/InstructionalVideo.aspx )
All the practice components can be performed by simulation as well as virtual labs.
Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).
Online Source: Labs for life, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia, Khan Academy, Labs for life.
Session Plan
Session 1
Topic: Microscopy
Study Materials:
Video- https://www.youtube.com/watch?v=SUo2fHZaZCU
Description- https://www.khanacademy.org/science/high-school-biology/hs-cells/hs-introduction-to-cells/a/microscopy
https://assetstore.unity.com/packages/templates/packs/cky-microscope-162720
Session 2
Topic: Phase–contrast microscopy
Study Materials:
Video- https://www.youtube.com/watch?v=fCZw4X7V5Pw
https://www.youtube.com/watch?v=HBP6SWZrilw
Slideshare-https://www.slideshare.net/rajpalchoudharyjat/phase-microscopy
Session 3
Topic: Fluorescence microscopy
Study Materials:
Video- https://www.youtube.com/watch?v=PCJ13LjncMc
https://www.youtube.com/watch?v=TtBja54wwXo
https://www.youtube.com/watch?v=dhAPQ_hdeP4
Slide share-https://www.slideshare.net/jyotsnaverma1232/fluorescence-microscopy-92806925
Session 4
Topic: Polarization microscopy
Study Materials:
Description- https://www.microscopyu.com/techniques/polarized-light/polarized-light-microscopy
Session 5
Topic: Scanning Electron microscopy (SEM)
Study Materials:
Video- https://app.lecturio.com/#/lecture/s/8432/37354
Session 6
Topic: Transmission Electron microscopy (TEM)
Study Materials:
Video- https://www.youtube.com/watch?v=D7vVg77tm_4
https://www.youtube.com/watch?v=t4hhdgJADE8
https://www.youtube.com/watch?v=j2A6KeWrqeM
https://www.youtube.com/watch?v=_kpN2jPjRYs&pbjreload=10
Slideshare-https://www.slideshare.net/gangahuvin/electron-microscopy-16995175
Session 7
Topic: Bacterial Colony Counter
Study Materials:
Video- https://www.youtube.com/watch?v=wUIZefiMXxI
Session 8
Session 9
Topic: Colorimeter- Principle and Instrumentation
Study Materials:
Video- https://www.youtube.com/watch?v=z78PBaAldRQ
Session 10
Topic: UV Spectrophotometry
Study Materials:
Video- https://www.youtube.com/watch?v=pxC6F7bK8CU
Session 11
Topic: Mass spectrophotometry
Study Materials:
Video- https://www.youtube.com/watch?v=J-wao0O0_qM
Session 12
Topic: Cytometry- Types, Flow cytometry and its applications
Study Materials:
Session 13
Topic: Flame photometry
Study Materials:
Video- https://www.youtube.com/watch?v=BBhuXOh9vOM
https://www.youtube.com/watch?v=1F6CxVF5I9g&t=23s
Session 14
Practice session:
Topic: Demontration of Microscope
Study Materials:
Session 15
Session 16
Session 17
Topic: Density gradient centrifuge
Study Materials:
Session 18
Session 19
Topic: Semi- automated Biochemistry Analyser
Study Materials:
Session 20
Practice session:
Topic: Demonstrate Semi- automated Biochemistry Analyser
Study Materials:
Session 21
Topic: Principle and Application of Fully Automated Biochemistry Analyser
Study Materials:
Video- http://labsforlife.in/InstructionalVideo.aspx
https://www.youtube.com/watch?v=LHweSWdoQ78
Session 22
Topic: Microtomy- Sectioning, Staining, Application
Study Materials:
Session 23
Practice session:
Topic: Procedure of Microtomy
Study Materials:
Session 24
Session 26
Practice session:
Topic: Incubator
Study Materials:
Session 27
Topic: Hot Air Oven
Study Materials:
Video- https://www.youtube.com/watch?v=yEsDVJQf4io
Session 28
Practice session:
Topic: Demonstration of Hot Air Oven
Study Materials:
Video- https://www.youtube.com/watch?v=yEsDVJQf4io
Session 30
Practice session:
Topic: Demonstration of Autoclave
Study Materials:
Case Studies
Case Studies
Our Main Teachers
Susmita Chakrabarty is currently working as an Assistant Professor in the School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes the field of Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique, Analytical Biochemistry & Clinical Biochemistry, etc. […]