Medical Instrumentation & Techniques

Teacher

Susmita Chakrabarty

Category

Core Courses

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Course Name : Medical Instrumentation & Techniques

Code(Credit) : CUTM1730 (2-2-0)

Course Objectives

  • To learn the principle, instrumentation & application of Microscopy
  • Principle, instrumentation & application of Centrifugation
  • Principle of Spectroscopy

Learning Outcomes

  • After completion of the course the student will be efficient in handling the microscopy equipment’s.
  • They will also be able to have idea about handling instruments like centrifuge, spectrophotometer, chromatography, flow cytometer, Automated and semi- automated Biochemistry analyzer.
  • The conceptual understanding of the subject provides opportunities for skill enhancement and scopes for higher education.

Course Syllabus

Module -I (12 hrs)

 

Microscopic techniques: Principle, Instrumentation, Specimen preparation and Application: Phase–contrast microscopy, fluorescene microscopy, polarization microscopy, electron microscopy (Scanning and Transmission);

Bacterial Colony Counter (Principle and working). Laminar Air Flow (Principle and working technique).

 

Practice: Demonstration of different Microscopes with their operation and maintain technique.

 

Module- II (14 hrs)

 

Colorimeter: Principle and Instrumentation;

Spectrophotometry: Ultraviolet, Mass spectrophotometry; Flame photometry.

Centrifugation: Principle; Preparative, Analytical, Density gradient centrifugation.

Cytometry: Types, Flow cytometry and its applications.

 

Practice: Operation, Demonstration and Quality control of Centrifuge, UV-Vis spectrometer, Colorimeter.

 

 

Module- III ( 14 hrs)

 

 

Microtomy: Sectioning, Staining. Application.

Principle and Application of: Fully Automated Biochemistry Analyser, Semi- automated Biochemistry Analyser, Coagulometer.

Principle, working and uses of: Incubator, Hot air oven, Autoclave.

 

Practice: Demonstration of Auto/ Semi auto Analyzer; Working procedure of microtome, Incubator, Hot air oven, autoclave and others

 

Suggested Readings:

  1. Wilson K and Walker J. (2010). Principles and Techniques of Biochemistry and Molecular Biology. 7th Ed., Cambridge University Press.

(e-Book link: https://www.pdfdrive.com/principles-and-techniques-of-biochemistry-and-molecular-biology-e174866056.html )

  1. Nelson DL and Cox MM. (2008). Lehninger Principles of Biochemistry, 5th Ed., W.H. Freeman and Company.

(e-Book link: https://www.pdfdrive.com/lehninger-principles-of-biochemistry-5th-edition-d164892141.html )

  1. Willey MJ, Sherwood LM & Woolverton C J. (2013). Prescott, Harley and Klein’s Microbiology. 7thEd., McGraw Hill.

(e-Book link: https://www.pdfdrive.com/prescott-harley-and-kleins-microbiology-7th-ed-e188166539.html )

  1. Labs for Life

(e-source link: http://labsforlife.in/InstructionalVideo.aspx )

5. e-Book link- https://books.google.co.in/books? id=z9SzvsSCHv4C&printsec=frontcover&dq=instrumentation&hl=en&sa=X&ved=2ahUKEwjipqrO347qAhUjwzgGHRomCNUQ6wEwAHoECAIQAQ#v=onepage&q=instrumentation&f=false

 

All the practice components can be performed by simulation as well as virtual labs.

Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).

Online Source: Labs for life, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia, Khan Academy, Labs for life.

Session 4

Topic: Polarization microscopy

Study Materials:

Description- https://www.microscopyu.com/techniques/polarized-light/polarized-light-microscopy

Session 8

Session 12

Topic: Cytometry- Types, Flow cytometry and its applications

Study Materials:

Video- http://labsforlife.in/InstructionalVideo.aspx

Session 14

Practice session:

Topic: Demontration of Microscope

Study Materials:

Notes-https://microbenotes.com/parts-of-a-microscope/

https://www.microscopeworld.com/t-parts.aspx

Session 15

Topic: Centrifugation Principle

Study Materials:

Video- https://www.youtube.com/watch?v=IhJNFGfsUus

https://www.youtube.com/watch?v=nX0NNfV8nWw

Session 16

Topic: Preparative centrifuge

Study Materials:

Video- https://www.youtube.com/watch?v=nX0NNfV8nWw

Session 17

Topic: Density gradient centrifuge

Study Materials:

Video- https://www.youtube.com/watch?v=_-YT44KP3do

Session 18

Topic: Analytical centrifuge

Study Materials:

Video- https://www.youtube.com/watch?v=A9wmCsMiy70

Session 19

Topic: Semi- automated Biochemistry Analyser

Study Materials:

Video- http://labsforlife.in/InstructionalVideo.aspx

https://www.youtube.com/watch?v=J9yoeVLxEvA

Session 20

Practice session:

Topic: Demonstrate Semi- automated Biochemistry Analyser

Study Materials:

Video- http://labsforlife.in/InstructionalVideo.aspx

https://www.youtube.com/watch?v=J9yoeVLxEvA

Session 22

Topic: Microtomy- Sectioning, Staining, Application

Study Materials:

Video- http://labsforlife.in/InstructionalVideo.aspx

Session 23

Practice session:

Topic: Procedure of Microtomy

Study Materials:

Video- http://labsforlife.in/InstructionalVideo.aspx

Session 26

Practice session:

Topic: Incubator

Study Materials:

Video- https://www.youtube.com/watch?v=ax_YGKmnt-M

https://www.youtube.com/watch?v=i074VMswTBk

Session 28

Session 30

Practice session:

Topic: Demonstration of Autoclave

Study Materials:

Video- https://www.youtube.com/watch?v=6YVqwnWnGdo

https://www.youtube.com/watch?v=j23s_cxwxSE

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      Miss Susmita Chakrabarty is currently working as a Lecturer in School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes field in Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique & Clinical Biochemistry etc. In her research, […]