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Module -I (12 hrs)
Microscopic techniques: Principle, Instrumentation, Specimen preparation and Application: Phase–contrast microscopy, fluorescene microscopy, polarization microscopy, electron microscopy (Scanning and Transmission);
Bacterial Colony Counter (Principle and working). Laminar Air Flow (Principle and working technique).
Practice: Demonstration of different Microscopes with their operation and maintain technique.
Module- II (14 hrs)
Colorimeter: Principle and Instrumentation;
Spectrophotometry: Ultraviolet, Mass spectrophotometry; Flame photometry.
Centrifugation: Principle; Preparative, Analytical, Density gradient centrifugation.
Cytometry: Types, Flow cytometry and its applications.
Practice: Operation, Demonstration and Quality control of Centrifuge, UV-Vis spectrometer, Colorimeter.
Module- III ( 14 hrs)
Microtomy: Sectioning, Staining. Application.
Principle and Application of: Fully Automated Biochemistry Analyser, Semi- automated Biochemistry Analyser, Coagulometer.
Principle, working and uses of: Incubator, Hot air oven, Autoclave.
Practice: Demonstration of Auto/ Semi auto Analyzer; Working procedure of microtome, Incubator, Hot air oven, autoclave and others
(e-source link: http://labsforlife.in/InstructionalVideo.aspx )
5. e-Book link- https://books.google.co.in/books? id=z9SzvsSCHv4C&printsec=frontcover&dq=instrumentation&hl=en&sa=X&ved=2ahUKEwjipqrO347qAhUjwzgGHRomCNUQ6wEwAHoECAIQAQ#v=onepage&q=instrumentation&f=false
All the practice components can be performed by simulation as well as virtual labs.
Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).
Online Source: Labs for life, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia, Khan Academy, Labs for life.
Topic: Phase–contrast microscopy
Topic: Fluorescence microscopy
Topic: Scanning Electron microscopy (SEM)
Topic: Transmission Electron microscopy (TEM)
Topic: Bacterial Colony Counter
Susmita Chakrabarty is currently working as an Assistant Professor in the School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes the field of Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique, Analytical Biochemistry & Clinical Biochemistry, etc. […]