Course Objectives

• Describe basic morphology, life cycle, pathogenesis, lab diagnosis and treatment of parasites and fungi.
• Perform appropriate laboratory techniques used in the processing of specimens and identification of parasites and fungi.
• Describe basic principle and procedures of isolation of fungus and parasies from clinical samples like stool, vaginal swab etc.
• Perform appropriate laboratory techniques used in the processing of specimens and identification of parasites and fungi.

Learning Outcomes

• Identification  of pathogenic parasite and fungus in disease diagnosis and treatment.
• The conceptual understanding of the subject provides opportunities for employability and scopes for higher education.
• To serve as a resource for the clinical laboratories professionals in the different region.

Course Syllabus

Module I

General Parasitology- Classification of medically important parasites, epidemiology of parasitic infections, immunology of human parasitic infections. Diagnostic parasitology- Systematic study of following parasites (Geographical distribution, habitat, morphology and life cycle, risk of infection, pathogenesis, laboratory diagnosis prophylaxis and serological diagnosis)

Practice

Collection & transport of specimens for examination of stool for parasites   identification. Examination of other body fluids for parasites identification. Concentration techniques for demonstration of Ova (Principles and applications). Routine Stool examination for detection of intestinal parasites.

Module-II

Protozoa – Intestinal amoeba, free living pathologic amoeba, giardia, trichomonas, balantidium, isospora, cryptosporidium, microspora,cyclospora Plasmodia, leishmania, trypanasoma, toxoplasma, babesia.

Helminthes– Cestodes – Taenia, Echinococus, Diphylobothrium, Hymenolepsis, Multiceps, Trematodes- Schistosoma, Fasciola, Fasciolepsis, Paragonimus, Clonorchis, Opisthorchis. Nematodes- Ascaris, Hookworm (Ancylostoma), Trichuris, Enterobius, Strongyloides, Filaria, Trichinella, Toxocara, Dracunculus, Biological vectors.

Practice

Identification of adult worms from model’s or slide’s. Identification of different parasites their morphology from slide’s. Culture techniques for parasites. Serological diagnostic methods.

Module-III

General Mycology – Fungus – Classification Fungal Structure & Morphology, Reproduction in fungi, Immunity to Fungal Infections. Culture Media in Mycology, Stains in Mycology. Normal fungal flora of human beings. Diagnostic Mycology - Epidemiology, Pathogenesis, Laboratory Diagnosis of Fungal Infections. Specimen collection, preservation, Transportation & Identification of Mycological Agent. Biochemical tests for fungal identification, Anti-fungal agents, invitro tests. Serological tests for mycotic infections. Use of laboratory animals in Mycology. Typing of fungi Preparation of fungal antigens & their standardization.

Practice

To prepare culture media used routinely in mycology. Diagnostic Methods in Mycotic Infections. Isolation and identification of fungus from clinical specimen. To perform all the staining techniques for identification of fungi.

Suggested Readings

1.Parasitology book by K.D. Chatterjee. 13th Ed.

2. Textbook of medical Parasitology.

Ebook Link

https://www.pdfdrive.com/textbook-of-medical-parasitology-textbook-of-medical-parasitology-e128716897.html

Online Tutorial Links

1. Diagnostic Mycology.

Elink-https://www.coursera.org/lecture/dermatology/fungal-infections-ZMX7J

2. Pathogenesis, Laboratory Diagnosis of Fungal Infections.

Elink- https://www.coursera.org/learn/epidemics

3.Identification of Mycological Agent.

https://vlab.amrita.edu/?sub=3&brch=76

4.Immunity to Fungal Infections.

https://www.coursera.org/lecture/bacterial-infections/2-2-chronic-infections-host-response-part-1-by-m-sc-ph-d-peter-ostrup-jensen-jzSKv

Simulation Links for Lab

1.Culture Media in Mycology.

https://vlab.amrita.edu/?sub=3&brch=76&sim=693&cnt=2

All the practice components can be performedby simulation as well as virtual labs.

Online Source: Udemy, Coursera, article library, NCBI,Study.com, You tube, Frontier, Febs,       unacademy, academia etc.

Session Plan

Session 1

Topic- General Parasitology, Classification of medically important parasite

Readings-http://whqlibdoc.who.int/publications/9241544104_(part1).pdf?ua=1

Ref. Book & Page- Parasitology by K D Chatterjee(13^th Ed), pg.15-chapter I, Parasitology by K D Chatterjee(13^th Ed), pg.143-chapter VI

Session 2

Topic- Epidemiology of parasitic infections

Readings- https://www.who.int/intestinal_worms/resources/who_trs_749/en/

https://www.who.int/intestinal_worms/epidemiology/en/

Session 3

Topic- Immunology of human parasitic infections.

Reference- https://www.coursera.org/lecture/epidemics/micro-and-macro-parasites-AiJ5S

Session 4

Topic- Intestinal amoeba, free living pathologic amoeba

Video link- https://www.youtube.com/watch?v=Klx0uDfCIRA

Ref. readings-

Parasitology by K D Chatterjee(13^th Ed), pg.18-41-chapter II

Session 5

Topic- Giardia

Video link- https://www.youtube.com/watch?v=cpu9PRIEYWY

Readings-

Parasitology by K D Chatterjee(13^th Ed), pg.47-48-chapter II

Session 6

Topic- Trichomonas

Video link- https://www.youtube.com/watch?v=5t6sHgRqavw

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.49-51-chapter II

Session 7

Topic- Balantidium

Video link- https://www.youtube.com/watch?v=xldAVfMfCmY

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.138-chapter IV

Session 8

Topic- Isospora

Video Link-https://www.youtube.com/watch?v=CyMvK1dumb8

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.128-chapter III

Session 9

Topic- Cryptosporidium

Video link-https://www.youtube.com/watch?v=RIFVlIrd8L0

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.134-chapter III

Session 10

Topic- Microspora

Video- https://www.youtube.com/watch?v=dRv2f9PdSgc

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.141-chapter V

Session 11

Topic- Cyclospora

Video link- https://www.youtube.com/watch?v=dVFRgmZpTfQ

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.135-chapter III

Session 12

Topic- Plasmodia

Video link- https://www.youtube.com/watch?v=1v55yg0RfoY

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.90-102-chapter III

Session 13

Topic- Leishmania

Video link- https://www.youtube.com/watch?v=wzjSmxThgJ0

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.64-68chapter II

Session 14

Topic- Trypanasoma

Video link-https://www.youtube.com/watch?v=fE3W1p7tMAk

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.51-chapter II

Session 15

Topic- Toxoplasma and Babesia

Video link- https://www.youtube.com/watch?v=0YLgo9GK5tE

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.129-chapter III

Parasitology by K D Chatterjee(13^th Ed), pg.136-chapter IV

Session 16

Topic- Taenia

Video link- https://www.youtube.com/watch?v=kNEYwXi-5_4

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.151-chapter VI

Session 17

Topic- Echinococus

Video link- https://www.youtube.com/watch?v=_rdb5j7jDhQ

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.165-chapter VI

Session 18

Topic- Diphylobothrium

Video link- https://www.youtube.com/watch?v=J9KVX__Hp10

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.147-chapter V

Session 19

Topic- Hymenolepsis

Video link- https://www.youtube.com/watch?v=ozO1oAbor-w

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.168-chapter VI

Session 20

Topic- Schistosoma

Video link- https://www.youtube.com/watch?v=Qoo9T3emHIQ

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.176-chapter VII

Session 21

Topic- Fasciola

Video link- https://www.youtube.com/watch?v=ZnJEgmWoOpw

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.186-chapter VII

Session 22

Topic- Paragonimus

Video link- https://www.youtube.com/watch?v=en1bKReS0bA

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.196-chapter VII

Session 23

Topic- Clonorchis

Video link- https://www.youtube.com/watch?v=BPXGNB0E_W4

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.192-chapter VII

Session 24

Topic- Ascaris

Video link- https://www.youtube.com/watch?v=4U3bIXaosh0

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.229-232-chapter VIII

Session 25

Topic- Plasmodia

Video link- https://www.youtube.com/watch?v=1v55yg0RfoY

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.90-102-chapter III

Session 26

Topic- Hookworm (Ancylostoma)

Video link- https://www.youtube.com/watch?v=QIWc-55IQzk

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.212-220-chapter VII

Session 27

Topic- Strongyloides

Video link- https://www.youtube.com/watch?v=5MuXsdIZSEc

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.208-212chapter VII

Session 28

Topic- Filaria

Video link- https://www.youtube.com/watch?v=9zMdg9PFCW0

Readings- Parasitology by K D Chatterjee(13^th Ed), pg.238-247chapter VIII

Session 29

Topic- General Mycology – Fungus – Classification Fungal Structure & Morphology

Video link- https://jamanetwork.com/journals/jamadermatology/fullarticle/542184

https://courses.lumenlearning.com/biology2xmaster/chapter/classification-of-fungi/

Session 30

Topic- Reproduction in fungi

Video link- https://www.youtube.com/watch?v=f3hFHFyzGjY

Session 31

Topic- Culture Media in Mycology  & Stains in Mycology

Video link- 

https://vlab.amrita.edu/?sub=3&brch=76&sim=693&cnt=2

https://www.youtube.com/watch?v=OMIF1Elr9i4

Session 32

Topic- Specimen collection, preservation, Transportation. Identification of Mycological Agent

Readings- https://www.cdc.gov/antibiotic-use/core-elements/collecting-cultures.html

Video Link-https://vlab.amrita.edu/?sub=3&brch=76

Session 33

Topic- Biochemical tests for fungal identification

Readings- https://www.slideshare.net/nasertadvi/antifungal-drugs-14709424

Case Studies

Case Studies