Microbiology
Teacher
Dr. Monali Priyadarsini Mishra
Category
Core Courses
Course Attendees
Still no participant
Course Reviews
Still no reviews
Course Name : CUTM1733-Microbiology
Code(Credit) : (3-2-0)
Course Objectives
- To know various Culture media and their applications and also understand various physical and chemical means of sterilization
- To know General bacteriology and microbial techniques for isolation of pure cultures of bacteria, fungi and virus
- To master aseptic techniques and be able to perform routine culture handling tasks safely and effectively
Learning Outcomes
- This study demonstrates the theory and practical skills in microscopy and their handling techniques and staining procedures.
- Understanding the details of microbial cell organelles.
- Provides knowledge on growth of microorganism.
- Provides knowledge Culturing microorganism.
Course Syllabus
Module -1
Microbiology: Definition, history, host- microbe relationship, and safety measures in a microbiology laboratory. Morphology of bacterial cell wall, Bacterial anatomy (Bacterial cell structure: including spores, flagella, pili and capsules). Sporulation. Classification of bacteria according to cell wall and shape (arrangement), Classification of micro-organisms. Growth and Nutrition of Microbes: General nutritional requirements of bacteria, Bacterial growth curve
Practice:
- Handling of Microscope
- To learn techniques for Inoculation of bacteria on culture media.
- To isolate specific bacteria from a mixture of organisms.
Module-2
Sterilization: Definition, sterilization by dry heat, moist heat (below, at & above 100⁰ C), Autoclave, Hot air oven, Radiation and Filtration, preventive measures, controls and sterilization indicators. Use of laminar flow in sterilization.
Antiseptics and Disinfectants: Definition, types, properties, mode of action and use of disinfectants and antiseptics, efficiency testing of disinfectants.
Practice:
- To demonstrate simple staining (Methylene blue)
- Bacterial identification: To demonstrate reagent preparation and procedure for Gram stain, Z-N staining, Capsule staining, Demonstration of flagella by staining methods, Spore staining, To demonstrate spirochetes by Fontana staining procedure
Module-3
Staining techniques: Methods of smear preparation, Gram stain, AFB stain, Albert’s stain and special staining for spore, capsule and flagella, Culture Media, Liquid and solid media, defined and synthetic media, routine laboratory media (basal, enriched, selective, enrichment, indicator, and transport media). Different Culture, media their preparation and uses in microbial growth.
Practice:
6. Biochemical tests for identification of bacteria
7. Preservation of stock cultures of bacteria
8. Antibiotic susceptibility test
Suggested References
- Textbook of Microbiology- Ananthanarayan & Paniker (10th Ed)
- Medical Microbiology-by Fritz H. Kayser et al
- Medical Laboratory Technology by Kanai Lal Mukherjee, Publisher Tata McGrawHill
- Microbiology (7th Ed)- by Prescott
- Practical Book of Medical Microbiology by Satish Gupta, Publisher JaypeeBrothers
- Medical Laboratory Manual for Tropical Countries Vol. I and II by Monica Cheesbrough
- Essential Medical Microbiology- by Rajesh Bhatia (4th Ed)
- Clinical laboratory methods and diagnosis by Gradwohls, 2000, Publisher Mosby
- Medical laboratory science theory and practice, J Ochei and Kolhatkar, 2002, publisher TBS
Session Plan
Session 1
Microbiology: Definition, history
Video 1: https://www.youtube.com/watch?v=hvvchbfw8-k
Video 2: https://www.youtube.com/watch?v=0vr2fcJZtdA
Presentation: https://www.slideshare.net/SalmanAli83/historyintroduction-scope-of-microbiology-siws
Session 2
host- microbe relationship
Video: https://www.youtube.com/watch?v=38nKkKH5WGo
Presentation: https://www.slideshare.net/Shilpak23/host-microbe-interaction
Session 3
safety measures in a microbiology laboratory
Video: https://www.youtube.com/watch?v=U8mLe8xh5yM
Presentation: https://www.slideshare.net/HiwrHastear/laboratory-safety-54228792
Session 4
Morphology of bacterial cell wall
Structure and function of Cell wall of bacteria
Video : https://www.youtube.com/watch?v=VkVLFbg0z5k
Presentation: https://www.slideshare.net/rajpalchoudharyjat/cell-wall-structure-and-function
Session 5
Bacterial anatomy (Bacterial cell structure: including spores, flagella, pili and capsules)
Video 1: https://www.youtube.com/watch?v=2xcdYlCjieI
Video 2: https://www.youtube.com/watch?v=r7CL0NwP0qM
Video 3: https://www.youtube.com/watch?v=VzqC7nag6KM
Presentation: https://www.slideshare.net/gohilsanjay3/structure-of-bacterial-cell-108802472
https://www.slideshare.net/ReenaKulshrestha/bacterial-anatomy-81692907
Session 6
Sporulation
Video: https://www.youtube.com/watch?v=NAcowliknPs
Presentation: https://www.slideshare.net/drKarthikvet/bacterial-spore
Session 7
Classification of bacteria according to cell wall and shape (arrangement)
Video 1: https://www.youtube.com/watch?v=wEvW1ZF7if4
Video 2:https://www.youtube.com/watch?v=Didrc3wJ3E8
Presentation: https://www.slideshare.net/shyrosh/classification-of-bacteria-49373508
Session 8
Classification of micro-organisms
Video 1 : https://www.youtube.com/watch?v=SgEkbDoPc5Y
Video 2 : https://www.youtube.com/watch?v=8IJRzcPC9wg
Presentation: https://www.slideshare.net/AhmadFaridAzizi/classification-of-microorganisms-lecture-2
Session 9
Growth and Nutrition of Microbes: General nutritional requirements of bacteria
Video: https://www.youtube.com/watch?v=jia7avWv4s4
Presentation: https://www.slideshare.net/RIZWANABBAS3/bacterial-nutrition-and-growth
Session 10
Bacterial growth curve
Video: https://www.youtube.com/watch?v=BsPKcHShGYw
Presentation: https://www.slideshare.net/9426401633/bacterial-growth-curves
Practice:
To learn techniques for Inoculation of bacteria on culture media.
Practice:
To isolate specific bacteria from a mixture of organisms.
Session 11
Sterilization: Definition, sterilization by dry heat
Video: https://www.youtube.com/watch?v=lmbvY-z1zt0
Presentation:
https://www.slideshare.net/saivivekkosaraju/sterilization-methods-32401134
Session 12
moist heat (below, at & above 100⁰ C), Autoclave
Video: https://www.youtube.com/watch?v=j23s_cxwxSE
Presentation: https://www.slideshare.net/zionpattres/moist-heat-sterilization
Session 13
Hot air oven
Video: https://www.youtube.com/watch?v=vvFDypILkTA
Presentation: https://www.slideshare.net/doctortvrao/sterilization-physical-methods
Session 14
Radiation and Filtration
Video: https://www.youtube.com/watch?v=t82WNvintDI
Presentation: http://large.stanford.edu/courses/2018/ph241/goronzy2/
Session 15
preventive measures
Session 16
controls and sterilization indicators
Video: https://www.youtube.com/watch?v=wcr21Uep-pc
Video: https://www.youtube.com/watch?v=HyBBj5m723Q
Presentation: https://www.slideshare.net/drsudeesh/sterilisation-30703657
Session 17
Use of laminar flow in sterilization
Video: https://www.youtube.com/watch?v=yhNBZ234GiY
Presentation: https://www.slideshare.net/bhavyasree528/laminar-air-flow-83734890
Session 18
Antiseptics and Disinfectants: Definition, types, properties
Video: https://www.youtube.com/watch?v=U08CFAJXKW0
Video:https://www.youtube.com/watch?v=ZBiw7wOM348
Presentation: https://www.slideshare.net/nasertadvi/antiseptics-and-disinfectants-15555738
Session 19
mode of action and use of disinfectants and antiseptics
Video: https://www.youtube.com/watch?v=_8MouGg-sr0
Presentation: https://www.slideshare.net/arkam741/antiseptic-and-disinfectant-88221452
Session 20
efficiency testing of disinfectants.
Video: https://www.youtube.com/watch?v=3q0uSc-Wa-4
Video: https://www.youtube.com/watch?v=T-EtvHUNTNg
Presentation: https://www.slideshare.net/kmv/testing-of-disinfectants
Practice:
To demonstrate simple staining (Methylene blue)
Practice:
Bacterial identification: To demonstrate reagent preparation and procedure for Gram stain, Z-N staining, Capsule staining, Demonstration of flagella by staining methods, Spore staining, To demonstrate spirochetes by Fontana staining procedure
Video 1: https://www.youtube.com/watch?v=c2BPE7wIK20
Video 2:https://www.youtube.com/watch?v=sxa46xKfIOY
Video 3:https://www.youtube.com/watch?v=NmfygqRQdeA
Video 4:https://www.youtube.com/watch?v=0yttbfBsSgk
Session 21
Staining techniques: Methods of smear preparation
Video: https://www.youtube.com/watch?v=c2BPE7wIK20
Presentation: https://www.slideshare.net/amjadkhanafridi4all/preparing-smears-for-staining
Session 22
Gram stain
Presentation:https://www.slideshare.net/doctortvrao/gram-staining-i-n-clinical-microbiology-by-drtvrao-md
Session 23
AFB stain
Presentation: https://www.slideshare.net/doctorrao/acid-fast-staining-in-tuberculosis
Session 24
Albert’s stain
Source: https://microbenotes.com/albert-staining/
Presentation: https://www.slideshare.net/RESHMASOMAN3/staining-techniques
Session 25
special staining for spore, capsule and flagella
Presentation: https://www.slideshare.net/RESHMASOMAN3/staining-techniques
Session 26
Session 27
Session 28
defined and synthetic media
Session 29
routine laboratory media (basal, enriched, selective, enrichment, indicator, and transport media)
Video: https://www.youtube.com/watch?v=suNU9xEHoDs
Presentation: https://www.slideshare.net/sasiprasad/culture-methods-9213006
Video: https://www.youtube.com/watch?v=EYHWgWiR6mU
Presentation: https://www.slideshare.net/musakhan9216/culture-media-used-in-microbiology
Session 30
Different Culture, media their preparation and uses in microbial growth.
Source: https://www.aladdin-e.com/up_files/docs/Types%20of%20culture%20media%20used%20in%20microbiology.pdf
Source: https://www.microrao.com/micronotes/culture_media.pdf
Practice:
Biochemical tests for identification of bacteria
Practice:
Preservation of stock cultures of bacteria
Case Studies
Case Studies
Our Main Teachers
Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]