PHYCOLOGY AND MICROBIOLOGY
Teacher
Dr. Pratibha Rani Deep
Category
Core Courses
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Phycology and Microbiology
Code(Credit) : CUTM1455 (3-2-1)
Course Objectives
- The course has been developed to provide the students basic knowledge about viruses, viroids, Prions, bacteria and algae.
- The students will explore the living world which is not visible to naked eye.
- The students will learn about how molecular entities like viruses have been used or can be exploited in future in the production of vaccines and medicines, disease diagnosis and in research
Learning Outcomes
Upon successful completion of this course, students will be able to:
- Acquire, articulate, retain and apply specialized language and knowledge relevant to microbiology.
- Acquire and demonstrate competency in laboratory safety and in routine and specialized microbiological laboratory skills applicable to microbiological research or clinical methods, including accurately reporting observations and analysis.
Course Syllabus
Module-I
Introduction to microbial world:
Microbial nutrition, growth and metabolism.
Economic importance of viruses with reference to vaccine production, role in research, medicine and diagnostics, as causal organisms of plant diseases. Economic importance of bacteria with reference to their role in agriculture and industry (fermentation and medicine).
Module-II
Viruses:
Discovery, physiochemical and biological characteristics; classification (Baltimore), general structurewith special reference to viroids and prions; replication (general account), DNA virus (T-phage), lytic and lysogenic cycle; RNA virus (TMV).
Practice 1: Electron micrographs/Models of viruses – T-Phage and TMV,
Practice 2:Line drawings/ Photographs of Lytic and Lysogenic Cycle
Module-III
Bacteria:
Discovery, general characteristics; Types-archaebacteria, eubacteria, wall-less forms (mycoplasma and spheroplasts); Cell structure; Nutritional types; Reproduction-vegetative, asexual and recombination (conjugation, transformation and transduction).
Practice 3: Electron micrographs of bacteria, binary fission, endospore, conjugation, root Nodule
Practice 4: Gram staining.
Practice 5: Endospore staining with malachite green using the (endospores taken from soil bacteria).
Practice 6: Study of Root Nodule bacteria
Module-IV
Algae:
General characteristics; Ecology and distribution; range of thallus organization; Cell structure and components; cell wall, pigment system, reserve food (of only groups represented in the syllabus), flagella; methods of reproduction;
Classification; criteria, system of Fritsch, and evolutionary classification of Lee (only upto groups);
Significant contributions of important phycologists (F.E. Fritsch, G.M. Smith, H.D. Kumar, M.O.P.Iyengar).Role of algae in the environment, agriculture, biotechnology and industry.
Module-V
Cyanophyta and Xanthophyta:
Ecology and occurrence; Range of thallus organization; Cell structure; Reproduction, Morphology and life-cycle of Nostoc and Vaucheria
Practice 7:Study of vegetative and reproductive structures of Nostoc, Vaucheria
Module-VI
Chlorophyta and Charophyta:
General characteristics; Occurrence; Range of thallus organization;
Cell structure; Reproduction, Morphology and life-cycles of Chlamydomonas, Volvox, Oedogonium, Coleochaete, Chara.
Practice 8:Study of vegetative and reproductive structures of Chlamydomonas (electron micrographs), Volvox,
Practice 9: Study of vegetative and reproductive structures of Oedogonium, Coleochaete,
Practice 10:Study of vegetative and reproductive structures of Chara .
Module-VII
Phaeophyta and Rhodophyta:
Characteristics; Occurrence; Range of thallus organization; Cell structure; Reproduction. Morphology and life-cycles of Ectocarpus, Fucus and Polysiphonia.
Practice 11:Study of vegetative and reproductive structures of Ectocarpus, Fucus
Practice 12:Study of vegetative and reproductive structures of Polysiphonia
Text Book:
Lee, R.E. (2008). Phycology, Cambridge University Press, Cambridge. 4th edition.
Wiley JM, Sherwood LM and Woolverton CJ. (2013) Prescott’s Microbiology. 9th Edition. McGraw Hill International.
Reference Book:
Kumar, H.D. (1999). Introductory Phycology. Affiliated East-West Press, Delhi. 2. Sahoo, D. (2000). Farming the ocean: seaweeds cultivation and utilization. Aravali International, New Delhi.
Campbell, N.A., Reece J.B., Urry L.A., Cain M.L., Wasserman S.A. Minorsky P.V., Jackson R.B. (2008). Biology, Pearson Benjamin Cummings, USA. 8th edition.
Pelczar, M.J. (2001) Microbiology, 5th edition, Tata McGraw-Hill Co, New Delhi.
Session Plan
Session 1
Session 2
Microbial nutrition, growth and metabolism.
https://www.youtube.com/watch?v=DfA-MHgi2ws
http://people.uleth.ca/~selibl/Biol3200/CourseNotes/GrowthCh6.pdf
Session 3 (Assignment 1)
Economic importance of viruses with reference to vaccine production, role in research, medicine and diagnostics
Session 4
Virus as causal organisms of plant diseases.
https://www.slideshare.net/zohaibhassan581187/plant-viruses-diseases-and-symptoms
https://www.slideshare.net/MohitMeena21/virus-and-virod-disease
Session 5 (Assignment 2)
Economic importance of bacteria with reference to their role in agriculture and industry (fermentation and medicine).
https://www.slideshare.net/sajigeorge64/economic-importance-of-bacteria-smg-205433689
Session 6
Module 2
Viruses: Discovery, physiochemical and biological characteristics;
https://www.bellarmine.edu/faculty/dobbins/Secret%20Readings/Lecture%20Notes%20313/Ch06_.pdf
https://www.slideshare.net/doctorrao/medical-virology-introduction
Session 8 (Assignment 3)
General structure with special reference to viroids and prions;
https://www.coakota.com/wp-content/uploads/2020/04/PPATH-622-Viroids-prions.pdf
http://eagri.org/eagri50/AMBE101/pdf/lec09.pdf
https://www.slideshare.net/dev9105/viroids
Session 9
Virus replication (general account),
https://www.slideshare.net/doctorrao/virus-structure-and-replication
https://samples.jblearning.com/0763729329/29329_CH03_046_069.pdf
https://www.youtube.com/watch?v=fH1zS7hlW54
DNA virus (T-phage)
https://www.slideshare.net/suganyakunju/bacteriophages-71259201
https://www.microrao.com/micronotes/bacteriophage.pdf
Session 10
Session 11 (Practice 1)
Line drawings/ Photographs of Lytic and Lysogenic Cycle
Session 13 (Practice 2)
Electron micrographs/Models of viruses – T-Phage and TMV,
Session 14 (Assignment 5)
Module 3
Bacteria: Discovery, general characteristics;
https://www.slideshare.net/Drzulcaifahmad/bacteria-16391639
http://samples.jbpub.com/9780763762582/62582_CH04_097_130.pdf
https://www.youtube.com/watch?v=S-JMug8vCFY
Session 15 (Practice 3)
Electron micrographs of bacteria, binary fission, conjugation, endospore, root Nodule
https://www.youtube.com/watch?v=oSd7Dlx56Xg
https://www.youtube.com/watch?v=WYKJYF7buE4
https://www.youtube.com/watch?v=j8_xoM8Wwgs
Session 16
Types-archaebacteria, eubacteria, wall-less forms (mycoplasma and spheroplasts);
http://www2.centralcatholichs.com/Bio1site/bacteria/Bacteria%20notes.PDF
https://www.slideshare.net/amjadkhanafridi4all/l-forms-protoplast-and-spheroplasts
https://www.slideshare.net/doctorrao/mycoplasma
Session 17 (Assignment 6)
Session 19
Reproduction-vegetative, asexual
https://patnawomenscollege.in/upload/e-content/Nutrition-and-Reproduction-in-Bacteria.pdf
Session 20 (Practice 5)
Endospore staining with malachite green (endospores taken from soil bacteria).
Session 21
Recombination (conjugation, transformation and transduction).
Session 23 (Assignment 7)
Module 4
Algae: General characteristics; Ecology and distribution;
https://www.slideshare.net/sardar1109/algae-notes-1
http://site.iugaza.edu.ps/elnabris/files/2011/02/6_Algae_-1_-General-Overview.pdf
https://biology4isc.weebly.com/uploads/9/0/8/0/9080078/plant_kingdom_notes.pdf
https://www.slideshare.net/jishashajik/algae-seminar-69517609
https://www.slideshare.net/NoeMendez9/distribution-and-abundance-of-algae
Session 24 (Assignment 8)
Role of algae in the environment, agriculture, biotechnology and industry.
https://www.slideshare.net/EasyBiologyClassEBC/algae-general-characters-by-easybiologyclasscom
https://www.slideshare.net/AbeerElhakem/biological-and-economic-importance-of-algae
https://pdfs.semanticscholar.org/8400/e9bae08896bad5f5dffb4b9fea8170a8d05e.pdf
https://www.youtube.com/watch?v=LNwUVET3CBQ
https://www.youtube.com/watch?v=N1dsN4npmCM
https://www.youtube.com/watch?v=KOVpp4CmgE4
Session 25
Range of thallus organization;flagella;methods of reproduction;
https://www.kcesmjcollege.in/ICT/Botany/Range%20of%20thallus%20in%20Algae.pdf
https://www.slideshare.net/16sam444/reproduction-in-algae
http://egyankosh.ac.in/bitstream/123456789/16678/1/Unit-4.pdf
Session 26
Cell structure and components;cell wall,pigment system, reserve food of Cyanophyta, xanthophyta, chlorophyta and charophyta
http://egyankosh.ac.in/bitstream/123456789/16675/1/Unit-3.pdf
http://oregonstate.edu/instruction/bot516/pdf/fall_05/Intro_Eukaryotic_algae.pdf
https://www.slideshare.net/EasyBiologyClassEBC/pigmentation-in-algae-ppt-by-easybiologyclass
Session 27
Classification; criteria, system of Fritsch,evolutionary classification of Lee (only upto groups);
https://www.plantscience4u.com/2014/04/fritsch-classification-of-algae.html#.Xte5F9SF6t8
http://site.iugaza.edu.ps/elnabris/files/2015/03/Algae-Introduction.pdf
Session 28
Significant contributions of important phycologists (F.E. Fritsch, G.M. Smith,).
https://www.slideshare.net/priyankakhare1/algae-classification-accorsing
https://www.amu.ac.in/emp/studym/99993037.pdf
Session 29 (Assignment 9)
Significant contributions of important phycologists- H.D. Kumar, M.O.P.Iyengar
https://www.ias.ac.in/article/fulltext/jbsc/029/03/0225-0230
Session 30
Module 5
Ecology and occurrence, Range of thallus organization; Cell structure of Cyanophyta.
https://www.slideshare.net/ShraddhaChavan1/cyanobacteria?next_slideshow=1
https://www.slideshare.net/gargskplantscience/cyanophyceaeblue-green-algae
https://www.slideshare.net/mksateesh/mks-cyanobacteria
http://oregonstate.edu/instruction/bot516/pdf/Cyanophyta.pdf
http://www.uobabylon.edu.iq/eprints/publication_11_26270_754.pdf
Session 31
Ecology and occurrence, Range of thallus organization; Cell structure of Xanthophyta.
https://www.fordham.edu/download/downloads/id/2989/45_xanthophyta_and_phaeophyta.pdf
https://www.slideshare.net/sohailriaz16/xanthophytaby-sohail-riaz-hashmi
Session 32
Cell structure, Morphology and life-cycle of Nostoc
Session 33
Cell structure, Morphology and life-cycle of Vaucheria
http://cms.gcg11.ac.in/attachments/article/115/Vaucheria.pdf
http://www.uobabylon.edu.iq/eprints/publication_12_28628_754.pdf
https://www.youtube.com/watch?v=-XbfXdUVDFI
https://www.youtube.com/watch?v=JPpF_xaMLds
https://www.youtube.com/watch?v=oEzHKdAu0-Ehttps://www.youtube.com/watch?v=oEzHKdAu0-E
Session 34 (Practice 7)
Study of vegetative and reproductive structures of Nostoc, Vaucheria,
https://www.youtube.com/watch?v=8a2YKnIKUIo&t=5s
Session 35
Module 6
General characteristics; Occurrence; Range of thallus organization; Cell structure of Chlorophyta:
https://courses.pbsci.ucsc.edu/eeb/bioe120/lecturenotes_2014/6_ChlorophytaI_14_2.pdf
http://www.uobabylon.edu.iq/eprints/publication_12_3410_754.pdf
Session 36
General characteristics; Occurrence; Range of thallus organization; Cell structure of Charophyta
https://www.slideshare.net/AfraFathima5/charophyta-125998140
http://www.uobabylon.edu.iq/eprints/publication_12_10833_614.pdf
Session 37
Reproduction, Morphology and life-cycles of Chlamydomonas,
https://www.slideshare.net/RabiaAziz6/chlamydomonas-assignment
Session 38
Reproduction, Morphology and life-cycles of Volvox
https://www.slideshare.net/MuhammedHaneef/volvox-154752242?
https://www.slideshare.net/saman91/volvox-19136939
https://www.slideshare.net/sumitachoudhary/volvox-ppt
Session 39 (Practice 8)
Study of vegetative and reproductive structures of Chlamydomonas (electron micrographs), Volvox
https://www.youtube.com/watch?v=EMNFZnDt75c
https://www.youtube.com/watch?v=xEBFFcwrBTU&t=12s
Session 40
Session 41
Reproduction; Morphology and life-cycles of Coleochaete
Session 42 (Practice 9)
Study of vegetative and reproductive structures of Oedogonium, Coleochaete
https://www.youtube.com/watch?v=mz_aj5QUqLA&t=23s
Session 45 (Practice 10)
Study of vegetative and reproductive structures of Chara, Vaucheria (Temporary Slide)
Session 46
Module 7
Characteristics; Occurrence; Range of thallus organization; Cell structure of Phaeophyta:
https://comenius.susqu.edu/biol/202/chromalveolata/heterokontae/phaeophyta/default.htm
https://www.slideshare.net/maratussolikhah96199/phaeophyta-35252984
Session 47
Characteristics; Occurrence; Range of thallus organization; Cell structure of Rhodophyta
http://oregonstate.edu/instruction/bot516/pdf/Rhodophyta.pdf
https://www.slideshare.net/priyankakhare1/rhodophyta-26946047
Session 48
Reproduction. Morphology and life-cycles of Ectocarpus
https://www.slideshare.net/NaveenAN4/type-study-of-ectocarpus
Session 49
Reproduction. Morphology and life-cycles of Fucus
Session 50 (Practice 11)
Study of vegetative and reproductive structures of Fucus, Ectocarpus
https://www.youtube.com/watch?v=SBVhdTCpFgk&t=19s
Session 51
Reproduction. Morphology and life-cycles of Polysiphonia
Session 52 (Practice 12)
Study of vegetative and reproductive structures of Polysiphonia
Case Studies
Case Studies
Our Main Teachers
Dr. Pratibha Rani Deep
Assisstant Professor in Botany, School of Applied Science, ( Bolangir Campus)
VIEW PROFILEDr. Pratibha R. Deep works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha India. She has received her M.Sc. (Botany) from Gangadhar Meher College (presently University), Sambalpur, India. She qualified GATE twice 2007 and 2012. She did her MTech in Applied Botany from IIT Kharagpur, […]