PHYCOLOGY AND MICROBIOLOGY

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Dr. Pratibha Rani Deep

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Core Courses

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Course Name :

Phycology and  Microbiology

Code(Credit) : CUTM1455 (3-2-1)

 

Course Objectives

  • The course has been developed to provide the students basic knowledge about viruses, viroids, Prions, bacteria and algae.

 

  • The students will explore the living world which is not visible to naked eye.

 

  • The students will learn about how molecular entities like viruses have been used or can be exploited in future in the production of vaccines and medicines, disease diagnosis and in research

Learning Outcomes

Upon successful completion of this course, students will be able to:  

  • Acquire, articulate, retain and apply specialized language and knowledge relevant to microbiology.

 

  • Acquire and demonstrate competency in laboratory safety and in routine and specialized microbiological laboratory skills applicable to microbiological research or clinical methods, including accurately reporting observations and analysis.

Course Syllabus

Module-I

Introduction to microbial world:

Microbial nutrition, growth and metabolism.

Economic importance of viruses with reference to vaccine production, role in research, medicine and diagnostics, as causal organisms of plant diseases. Economic importance of bacteria with reference to their role in agriculture and industry (fermentation and medicine).

 

Module-II

Viruses:

Discovery, physiochemical and biological characteristics; classification (Baltimore), general structurewith special reference to viroids and prions; replication (general account), DNA virus (T-phage), lytic and lysogenic cycle; RNA virus (TMV).

Practice 1: Electron micrographs/Models of viruses – T-Phage and TMV,

Practice 2:Line drawings/ Photographs of Lytic and Lysogenic Cycle

 

Module-III

 Bacteria:

Discovery, general characteristics; Types-archaebacteria, eubacteria, wall-less forms (mycoplasma and spheroplasts); Cell structure; Nutritional types; Reproduction-vegetative, asexual and recombination (conjugation, transformation and transduction).

Practice 3: Electron micrographs of bacteria, binary fission, endospore, conjugation, root Nodule

Practice 4: Gram staining.

Practice 5: Endospore staining with malachite green using the (endospores taken from soil bacteria).

Practice 6: Study of Root Nodule bacteria

 

Module-IV

 Algae:

General characteristics; Ecology and distribution; range of thallus organization; Cell structure and components; cell wall, pigment system, reserve food (of only groups represented in the syllabus), flagella; methods of reproduction;

Classification; criteria, system of Fritsch, and evolutionary classification of Lee (only upto groups);

Significant contributions of important phycologists (F.E. Fritsch, G.M. Smith, H.D. Kumar, M.O.P.Iyengar).Role of algae in the environment, agriculture, biotechnology and industry.

 

Module-V

Cyanophyta and Xanthophyta:

Ecology and occurrence; Range of thallus organization; Cell structure; Reproduction, Morphology and life-cycle of Nostoc and Vaucheria  

Practice 7:Study of vegetative and reproductive structures of Nostoc, Vaucheria

 

Module-VI

Chlorophyta and Charophyta:

General characteristics; Occurrence; Range of thallus organization;

Cell structure; Reproduction, Morphology and life-cycles of Chlamydomonas, Volvox, Oedogonium, Coleochaete, Chara.

Practice 8:Study of vegetative and reproductive structures of Chlamydomonas (electron micrographs), Volvox,

Practice 9: Study of vegetative and reproductive structures of Oedogonium, Coleochaete,

Practice 10:Study of vegetative and reproductive structures of Chara .

 

Module-VII

Phaeophyta and Rhodophyta:

Characteristics; Occurrence; Range of thallus organization; Cell structure; Reproduction. Morphology and life-cycles of Ectocarpus, Fucus and Polysiphonia.

 

Practice 11:Study of vegetative and reproductive structures of Ectocarpus, Fucus 

Practice 12:Study of vegetative and reproductive structures of Polysiphonia

Text Book:

Lee, R.E. (2008). Phycology, Cambridge University Press, Cambridge. 4th edition.

 

Wiley JM, Sherwood LM and Woolverton CJ. (2013) Prescott’s Microbiology. 9th Edition. McGraw Hill International.

Reference Book:

Kumar, H.D. (1999). Introductory Phycology. Affiliated East-West Press, Delhi. 2. Sahoo, D. (2000). Farming the ocean: seaweeds cultivation and utilization. Aravali International, New Delhi.

 

Campbell, N.A., Reece J.B., Urry L.A., Cain M.L., Wasserman S.A. Minorsky P.V., Jackson R.B. (2008). Biology, Pearson Benjamin Cummings, USA. 8th edition.

 

Pelczar, M.J. (2001) Microbiology, 5th edition, Tata McGraw-Hill Co, New Delhi.

Session 3 (Assignment 1)

Session 11 (Practice 1)

Line drawings/ Photographs of Lytic and Lysogenic Cycle

https://www.youtube.com/watch?v=hFwA0aBX5bE

Session 13 (Practice 2)

Electron micrographs/Models of viruses – T-Phage and TMV,

https://www.youtube.com/watch?v=H1B-h7LwrZY

Session 18 (Practice 4)

Session 20 (Practice 5)

Endospore staining with malachite green (endospores taken from soil bacteria).

https://www.youtube.com/watch?v=FqbHsWd-sOo

Session 22 (Practice 6)

Study of Root Nodule bacteria

https://www.youtube.com/watch?v=T4kzn6KmXX8

Session 34 (Practice 7)

Session 36

General characteristics; Occurrence; Range of thallus organization; Cell structure of Charophyta

https://www.slideshare.net/AfraFathima5/charophyta-125998140

http://www.uobabylon.edu.iq/eprints/publication_12_10833_614.pdf

Session 39 (Practice 8)

Session 42 (Practice 9)

Session 45 (Practice 10)

Study of vegetative and reproductive structures of  Chara, Vaucheria (Temporary Slide)

https://www.youtube.com/watch?v=0gzCGjhisXY&t=93s

https://www.youtube.com/watch?v=oEzHKdAu0-E&t=51s

Session 48

Session 50 (Practice 11)

Session 52 (Practice 12)

Study of vegetative and reproductive structures of Polysiphonia

https://www.youtube.com/watch?v=tOrVG_m2ej0

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Our Main Teachers

Dr. Pratibha Rani Deep

Assisstant Professor in Botany, School of Applied Science, ( Bolangir Campus)
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Dr. Pratibha R. Deep works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha India. She has received her M.Sc. (Botany) from Gangadhar Meher College (presently University), Sambalpur, India. She qualified GATE twice 2007 and 2012. She did her MTech in Applied Botany from IIT Kharagpur, […]