Microbiology

Teacher

Dr. Monali Priyadarsini Mishra

Category

Core Courses

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Course Name : Microbiology

Code(Credit) : (3-2-0)

Course Objectives

  • To know various Culture media and their applications and also understand various physical and chemical means of sterilization
  • To know General bacteriology and microbial techniques for isolation of pure cultures of bacteria, fungi and virus
  • To master aseptic techniques and be able to perform routine culture handling tasks safely and effectively

Learning Outcomes

  • This study demonstrates the theory and practical skills in microscopy and their handling techniques and staining procedures.
  • Understanding the details of microbial cell organelles.
  • Provides knowledge on growth of microorganism.
  • Provides knowledge Culturing microorganism.

Course Syllabus

Module -1

Microbiology: Definition, history, host- microbe relationship, and safety measures in a microbiology laboratory. Morphology of bacterial cell wall, Bacterial anatomy (Bacterial cell structure: including spores, flagella, pili and capsules). Sporulation. Classification of bacteria according to cell wall and shape (arrangement), Classification of micro-organisms. Growth and Nutrition of Microbes: General nutritional requirements of bacteria, Bacterial growth curve

Practice:

  1. Handling of Microscope
  2. To learn techniques for Inoculation of bacteria on culture media.
  3. To isolate specific bacteria from a mixture of organisms. 

Module-2 

Sterilization: Definition, sterilization by dry heat, moist heat (below, at & above 100⁰ C), Autoclave, Hot air oven, Radiation and Filtration, preventive measures, controls and sterilization indicators. Use of laminar flow in sterilization.

Antiseptics and Disinfectants: Definition, types, properties, mode of action and use of disinfectants and antiseptics, efficiency testing of disinfectants.

Practice:

  1. To demonstrate simple staining (Methylene blue)
  2. Bacterial identification: To demonstrate reagent preparation and procedure for Gram stain, Z-N staining, Capsule staining, Demonstration of flagella by staining methods, Spore staining, To demonstrate spirochetes by Fontana staining procedure

Module-3                                      

Staining techniques: Methods of smear preparation, Gram stain, AFB stain, Albert’s stain and special staining for spore, capsule and flagella, Culture Media, Liquid and solid media, defined and synthetic media, routine laboratory media (basal, enriched, selective, enrichment, indicator, and transport media). Different Culture, media their preparation and uses in microbial growth.

Practice:

6. Biochemical tests for identification of bacteria

7. Preservation of stock cultures of bacteria

8. Antibiotic susceptibility test

Suggested Readings

  1. Textbook of Microbiology- Ananthanarayan & Paniker (10th Ed)
  2. Medical Microbiology-by Fritz H. Kayser et al
  3. Medical Laboratory Technology by Kanai Lal Mukherjee, Publisher Tata McGrawHill
  4. Microbiology (7th Ed)- by Prescott
  5. Practical Book of Medical Microbiology by Satish Gupta, Publisher JaypeeBrothers
  6. Medical Laboratory Manual for Tropical Countries Vol. I and II by Monica Cheesbrough
  7. Essential Medical Microbiology- by Rajesh Bhatia (4th Ed)
  8. Clinical laboratory methods and diagnosis by Gradwohls, 2000, Publisher Mosby
  9. Medical laboratory science theory and practice, J Ochei and Kolhatkar, 2002, publisher TBS

Session Plan

Session 1

Session 2

Session 3

Session 4

Morphology of bacterial cell wall

Structure and function of Cell wall of bacteria

Video : https://www.youtube.com/watch?v=VkVLFbg0z5k

Presentation: https://www.slideshare.net/rajpalchoudharyjat/cell-wall-structure-and-function

Session 5

Session 6

Session 7

Classification of bacteria according to cell wall and shape (arrangement)

Video 1: https://www.youtube.com/watch?v=wEvW1ZF7if4

Video 2:https://www.youtube.com/watch?v=Didrc3wJ3E8

Presentation: https://www.slideshare.net/shyrosh/classification-of-bacteria-49373508

Session 8

Session 9

Growth and Nutrition of Microbes: General nutritional requirements of bacteria

Video: https://www.youtube.com/watch?v=jia7avWv4s4

Presentation: https://www.slideshare.net/RIZWANABBAS3/bacterial-nutrition-and-growth

Session 10

Practice:

Handling of Microscope

Video: https://www.youtube.com/watch?v=pujis3QsA_I

.

Practice:

To learn techniques for Inoculation of bacteria on culture media.

Video: https://www.youtube.com/watch?v=370xxVcujyg

Practice:

To isolate specific bacteria from a mixture of organisms.

Video: https://www.youtube.com/watch?v=uPveNOnmQxI&t=4s

Session 11

Sterilization: Definition, sterilization by dry heat

Video: https://www.youtube.com/watch?v=lmbvY-z1zt0

Presentation:

https://www.slideshare.net/saivivekkosaraju/sterilization-methods-32401134

Session 12

moist heat (below, at & above 100⁰ C), Autoclave

Video: https://www.youtube.com/watch?v=j23s_cxwxSE

Presentation: https://www.slideshare.net/zionpattres/moist-heat-sterilization

Session 13

Session 14

Session 15

preventive measures

Video: https://www.youtube.com/watch?v=84CydmuHXD8

Session 16

Session 17

Session 18

Session 19

mode of action and use of disinfectants and antiseptics

Video: https://www.youtube.com/watch?v=_8MouGg-sr0

Presentation: https://www.slideshare.net/arkam741/antiseptic-and-disinfectant-88221452

Session 20

Practice:

To demonstrate simple staining (Methylene blue)

Video:  https://www.youtube.com/watch?v=8ODeT9DLHKI

Practice:

Bacterial identification: To demonstrate reagent preparation and procedure for Gram stain, Z-N staining, Capsule staining, Demonstration of flagella by staining methods, Spore staining, To demonstrate spirochetes by Fontana staining procedure

Video 1: https://www.youtube.com/watch?v=c2BPE7wIK20

Video 2:https://www.youtube.com/watch?v=sxa46xKfIOY

Video 3:https://www.youtube.com/watch?v=NmfygqRQdeA

Video 4:https://www.youtube.com/watch?v=0yttbfBsSgk

Video 5:https://www.youtube.com/watch?v=mZGEhql02_I

Video 6: https://www.youtube.com/watch?v=cXYfT5hSLoQ

Session 21

Session 22

Session 23

Session 24

Session 25

special staining for spore, capsule and flagella

Presentation: https://www.slideshare.net/RESHMASOMAN3/staining-techniques

Session 26

Session 27

Session 28

defined and synthetic media

Video: https://www.youtube.com/watch?v=_HDPGs4HLgQ

Session 29

routine laboratory media (basal, enriched, selective, enrichment, indicator, and transport media)

Video: https://www.youtube.com/watch?v=suNU9xEHoDs

Presentation: https://www.slideshare.net/sasiprasad/culture-methods-9213006

Video: https://www.youtube.com/watch?v=EYHWgWiR6mU

Presentation: https://www.slideshare.net/musakhan9216/culture-media-used-in-microbiology

Session 30

Practice:

Biochemical tests for identification of bacteria

Video: https://www.youtube.com/watch?v=Eabt1kgy3ZQ

https://www.youtube.com/watch?v=fz_xksoQOuo&t=36s

Practice:

Preservation of stock cultures of bacteria

Video: https://www.youtube.com/watch?v=kXxmE5p-wF8&t=139s

Practice:

Antibiotic susceptibility test

Video: https://www.youtube.com/watch?v=XhyMUHVbrlI&t=322s

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    Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]